HPV16 E6/E7 Negatively Affect Radiosensitivity of Lung Cancer Cells

Objective Lung cancer cells associated with radioresistance are likely to give rise to local recurrence and distant metastatic relapse,but little is known about its underlying mechanisms.In the present paper,the effects of the HPV16 E6 and HPV16 E7 oncoprotein on the radiosensitivity of lung cancer cell lines were investigated.Methods The HPV16 E6 or HPV16 E7 oncoprotein was expressed by a transient transfection with pcDNA3-HPV16 E6 or pcDNA3-HPV16 E7 expression vector.Human lung cancer H2179 cells and mouse lung cancer Lewis cells were exposed to a γ-ray radiation source,cellular survival was evaluated by using a colony formation assay.The expression of HPV16 oncoproteins E6/E7,extracellular signal-regulated kinases 1/2(ERK1/2) and AKT signaling was determined by Western blot assay.VEGF secretion was determined by ELISA.Results Both HPV16 oncoproteins E6 and E7 significantly decreased radiosensitivity of H2179 cells,associated with a promotion of the ERK1/2 and AKT phosphorylation.A decrease of reactive oxygen species(ROS) and an increase of VEGF levels were observed in the cells expressing the HPV16 oncoproteins E6 and E7.Furthermore,a similar reduction of radiosensitivity mediated by the HPV16 oncoproteins E6 and E7 was also observed in a mouse lung cancer Lewis cells.Conclusion The findings indicate that the HPV16 oncoproteins E6 and E7 negatively affects susceptibility of lung cancer cells to radiotherapy via regulation of the ERK1/2 and Akt signaling pathway and VEGF expression.     

武警浙江总队医院30年帮困奏响和谐强音

本刊讯 武警浙江总队医院在认真做好部队官兵医疗保障的同时,连续30年开展为驻地困难家庭送温暖、送健康、献爱心活动,赢得了群众高度赞誉.今年春节前夕,该院在去年帮扶310户困难家庭的基础上,进一步加大了帮扶力度,出资22万余元,对嘉兴市区410户困难家庭开展献爱心行动.     

Elscint 2400 Elect CT结构改装探讨

球管是CT机最昂贵的消耗部件之一,其使用好坏,直接影响医院的经济效益和社会效益.随着放射科质量控制与质量保证工作的深化,许多单位已意识到质量控制与质量保证工作的开展在延长CT球管寿命及由其带来的巨大经济收益方面所产生的实际意义,同时在如何延长球管使用寿命方面探讨出了一些可行的经验[1,2].     

豚鼠耳石修复再生的超微结构及元素成分动态变化

目的 观察成熟耳石损伤后修复再生的形态学和元素变化特点,为深入认识其修复再生的规律和机理提供依据.方法 给予过载刺激(10 Gy 5 min)造成18只豚鼠耳石的损伤破坏,用扫描电镜(scanning electron microscopy,SEM)分别观察过载刺激后1 h、2 d、8 d时其椭圆囊囊斑,用X射线显微能谱分析技术(electron probe X-ray microanalysis,EPMA)测量不同形态类别的耳石元素构成.结果 过载刺激后1 h正常耳石形态消失,被大量球状物代替,2 d时球状物逐渐矿化,8 d时哑铃状耳石出现并与矿化球状物共存,此外各时期均可见各种耳石的变性.EPMA结果显示,变性耳石Ca元素含量显著增高和P元素含量显著降低.另外,球状物在由初期向成熟矿化期转化过程中,表现出P元素含量逐渐增加和Ca元素含量逐渐减少的趋势,但未达到统计学显著水平.结论 成熟耳石损伤破坏后可以修复再生,该过程中形态学的变化伴随着元素构成的变化.     

婴儿骨皮质增生症一例

例资料 患者,男,9个月.因出生后时常哭闹不安独坐不稳就诊.患儿为孕8个月早产.查体:神清,体温37.6℃,心肺听诊无异常.腹软,双下肢不能伸直,右侧明显,踝阵挛(-),双下肢略肿胀,皮肤颜色无异常,皮温不高.肌力张略高,肌力Ⅳ级.X线示如图1.实验室检查:白细胞10.8×109/l,红细胞4.62×1012/l,血红蛋白90 g/l,C反应蛋白21.70 mg/l(正常参考值0.1～10.0 ng/l),血沉29 mm/1h,青霉素皮试( ).     

双侧先天性桡骨小头脱位一例

病例资料患者,男,8岁。因挫伤右前臂到骨科就诊,发现右前臂肘侧表皮擦伤,轻微红肿及压痛,无轴向扣击痛,无明显活动受限,触及双侧肘窝外侧桡骨小头部前突,骨性硬,与桡骨一致性相连续,关节活动无功能障碍。双肘正侧位X线片示两肘关节桡骨小头呈对称性明显向前移位(图1、2),桡骨中     

闭孔疝1例

患者 女,70岁.以腹胀3 d收住消化内科,多次DR片提示肠梗阻,保守治疗10 d肠梗阻未能解除.既往肝硬化失代偿. CT扫描显示:右侧耻骨肌和闭孔外肌间可见大小约2 cm×3 cm软组织影,内呈低密度,并与腹内肠管相通,近端肠管明显扩张,伴有液平(图1,2).CT诊断为:闭孔疝,肠梗阻.转入外科治疗.     

Learning From Biomarkers in Victims Accidentally Exposed to Ionizing Radiation

Biomarkers,such as chromosome aberration and micronuclei assays,prove to be reliable for facilitating clinical diagnosis in radiation accidents.In a radiation accident in India,chromosomal aberration,γ-H2AX,as well as other blood markers,were detected in accidentally exposed victims.This multi-parametric approach aided in confirming that individuals had been exposed by ionizing radiation.However,doses were impossible to estimate because of a 30-day delay in accident awareness.Exposure dose for victims was estimated using a dose-response curve previously established.Dose estimation,blood cell depletion kinetics,and no appearance of prodromal symptoms suggested that doses of exposure were low.Hematologic investigation,sampling time,and chromosome aberration scoring were all proposed according to data from the victims exposed to 60Co.Finally,knowledge regarding chromosome aberration analysis and the importance of international co-operation and assistance should be shared from this accident.     

手术与MEBO外用治疗感染性嵌甲

目的：探讨足趾感染性嵌甲的治疗方法。方法：178例226个感染性嵌甲，即时手术，大范围楔形切除嵌甲及感染性组织，手术前后不用抗生素，骨外露的切口涂用MEBO治疗；观察指标：病人疼痛反应。化脓感染的控制，伤口愈合，保留甲的美观程度，复发率及医疗费用。结果：病人术后无痛或轻微疼痛，无须用止痛药物，不影响睡眠，可穿宽松鞋自由走动；术后3d可见新生肉芽组织填满切口，1周左右可见新生白色的上皮组织完全覆盖切口，继续应用MEBO1周～2周，全部愈合，愈合后无明显瘢痕，保留甲大而美观；切口无化脓感染，无末节趾骨骨髓炎发生。结论：手术结合MEBO是根治感染性嵌甲的有效方法。     

纳米材料毒理学研究进展

纳米材料作为一种新型材料已被广泛应用于机械、电子、纺织、化工、环保、医药和军事装备等领域,同时其生物安全性问题也引起了人们的关注.有研究表明,纳米材料可以通过呼吸道、皮肤、消化道等途径进入人体,并导致机体不同水平的毒性损伤.本文归纳介绍纳米材料毒理学研究的现状,并分析提出了该研究领域中值得关注的问题,以期为纳米材料的生物安全性研究提供参考.     

成骨不全症临床X线诊断(附8例报告)

成骨不全症以称脆骨症,是一种少见的遗传中胚层发育障碍造成骨骼脆性增加及胶原蛋白代谢紊乱为特征的结缔组织异常性疾病.     

术前介入性栓塞治疗罕见巨大子宫肌瘤一例

患者女,40岁.因"腹痛,B超检出盆腔包块1 d"于2006年8月19日入院.入院体检:腹膨隆,张力大,腹部可扪及一巨大肿块,质较硬,活动差.专科检查:子宫如孕36周,上至剑突下,质较硬,边界清,活动差,轻压痛.     

MRCP在梗阻性黄疸性疾病中的应用价值及进展

梗阻性黄疽是临床常见病症之一,其发生原因复杂多样,对病因的定位、定性诊断是临床治疗的关键.MRCP作为一种胰胆管成像新技术以其准确、无创、成功率高等诸多优点受到临床及影像医师的关注,本文即就MRCP的原理、应用现状及其与相关影像学方法的比较研究做一综述.     

竹笋味美又疗疾

竹笋,即竹的嫩芽茎,古人称之为"竹肉"、"竹胎"、"竹芽"。竹笋可分为冬笋、春笋、鞭笋3类:冬季藏在土中的为冬笋,色泽洁白,肉质细嫩,鲜美无比,有"笋中皇后"之誉;春天     

Dicer蛋白的晶体结构及其功能

RNA干扰(RNA interference,RNAi)是一种进化上保守的抵御转基因或外来病毒侵犯的防御机制,为与靶基因的转录产物mRNA存在同源互补序列的内源性或外源性双链RNA(double stranded RNA,dsRNA).     

阳性MR淋巴造影检测隐匿转移性淋巴结的实验研究

目的 初步探索皮下应用Gd-DTPA-白蛋白的阳性磁共振淋巴造影检测隐匿转移性淋巴结的价值.方法 建立腘窝隐匿转移性淋巴结新西兰兔模型,分析隐匿转移性淋巴结在平扫及皮下注射0.10 mmol/肢Gd-DTPA-白蛋白后24 h时的MRI特征,并与病理检查对照.结果 12只兔的腘窝隐匿转移性淋巴结模型成功建立,其淋巴结大小为(6.7±0.2)mm.平扫时隐匿转移性腘窝淋巴结表现为T1WI上等信号,T2WI上高信号,与正常淋巴结相似.Gd-DTPA-白蛋白增强24 h,正常淋巴结呈明显、均匀的强化,而隐匿转移性淋巴结呈不均匀强化,强化形式有环状(4枚淋巴结)、云絮状(3枚)和不规则偏心"充盈缺损"(3枚),另有2枚淋巴结不强化.在脂肪抑制T1WI像上淋巴管呈明亮高信号,转移性淋巴结的引流淋巴管明显迂曲扩张.结论 皮下应用Gd-DTPA-白蛋白的阳性MR淋巴造影可清晰显示淋巴结和淋巴管,可用于检测隐匿转移性淋巴结.     

补充维生素B2对急性低氧暴露小鼠血浆代谢组的影响

目的 观察补充维生素B2对急性低氧暴露小鼠外周血代谢组的影响.方法 采用随机数字表法将35只雄性昆明小鼠随机分为:正常对照组,低氧对照组,2倍、4倍及8倍维生素B2补充组,每组7只.以相应饲料(维生素B2含量分别为6 mg/kg、12 mg/kg、24 mg/kg和48 mg/kg)喂养2周后,除正常对照组外,其它各组均在模拟6000 m高度停留8 h,采集血浆,以核磁共振的方法分析其代谢组变化.结果 急性低氧暴露后,各组动物血浆代谢组在得分图中呈聚类型分布,且有先分离后同归的代谢模式变化轨迹,显示出补充不同剂量维生素B2后小鼠血浆代谢组逐渐恢复的趋势.代谢模式产生差别的原因是脂类、乳酸、丙氨酸、N-乙酰糖蛋白、谷氨酸、胆碱、牛磺酸、糖、肉碱、甘氨酸和肌酐等物质的水平发生了变化,表明相关的代谢途径发生了变化.结论 补充维生素B2使急性低氧暴露机体碳水化合物、脂肪、蛋白质代谢发生改变,而维生素B2可以改善碳水化合物代谢,并可能通过肉碱间接调节了脂肪代谢;同时还发现一些氨基酸代谢发生显著变化. Abstract： Objective To observe the effects of vitamin B2 supplementation on plasma metabonome of the mice which exposed to acute hypoxia. Methods Thirty-five male Kunming mice were randomly and averagely divided into 5 groups, among which the control and hypoxia control groups were fed the fodder containing 6, 12, 24, 48 mg/kg vitamin B2 respectively. All groups were exposed to simulated hypoxia environment (equivalent to 6000 meters above sea level) for 8 hours except control group. Nuclear magnetic resonance spectrometer was used to test the change of metabonome from collected plasma. Results After acute hypoxia exposure, the metabonome pattern in all groups showed clustering distribution in scores plot and the metabonome changes were along the trail from segregation to regression. These indicated a gradual recover tendency on plasma metabonome when different doses of vitamin B2 supplemented. The changes of lipids, lactic acid,alanine, N-acetyl-glycoprotein (NAC), glutamate, choline, taurine, glucose, carnitine, glycine and creatinine were among the factors contributing significantly to the difference of metabonome pattern and indicated the changes of related metabolic pathway. Conclusions The metabonomes of carbohydrate, lipid and protein of acute hypoxia mice are improved by vitamin B2 supplementation.Vitamin B2 is helpful to improve carbohydrate metabolism and indirectly accommodate lipid metabolism through carnitine. Vitamin B2 supplementation also results in the significant change of amino acid metabonome.     

基于置信区间宽度的样本含量估计

目的:介绍基于置信区间宽度的样本含量估计方法.方法:根据基于置信区间宽度的样本含量估计的原理,在分析基于该原理的样本含量估计值的近似算法的基础上提出新的算法,并对不同算法的计算结果进行比较.结果:指出了在求取基于置信区间宽度的样本含量估计值时,用近似法会低估样本含量,导致对总体均数的估计精度降低,而采用常规迭代法却不能确保样本标准差s与置信区间宽度L的比值s/L很小的情况下前后两次求得的n趋向稳定的数值,从而提出了基于置信区间宽度的样本含量估计值的一种改进算法--基于近似值的定向微调尝试法,并在近似法和基于近似值的定向微调尝试法的基础上提出了求取基于置信区间宽度的样本含量估计值的另一种改进算法--近似值校正法.在置信区间的覆盖率保持不变的情况下,按改进算法计算得到的样本含量抽样对总体均数的估计精度要比按近似法计算得到的样本含量抽样对总体均数的估计精度高.结论:建立了两种满足对总体均数估计精度要求的求取基于置信区间宽度的样本含量估计值的改进算法,即基于近似值的定向微调尝试法和近似值校正法.     

骨骼病变的MR功能成像应用进展

MR灌注成像、MR弥散加权成像和MR波谱作为广义的MR功能成像方法,可以显示人体微观水平的影像或数据,能够从不同角度反映组织的生理、生化和代谢方面的信息.本文对三种MR功能成像方法在骨骼病变诊断及良、恶性鉴别中的应用进行综述.