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Objective:To investigate whether blood-brain barrier(BBB)served a key role in the edema-relief effect of bloodletting puncture at hand twelve Jing-well points(HTWP)in traumatic brain injury(TBI)and the potential molecular signaling pathways.Methods:Adult male Sprague-Dawley rats were assigned to the shamoperated(sham),TBI,and bloodletting puncture(bloodletting)groups(n=24 per group)using a randomized number table.The TBI model rats were induced by cortical contusion and then bloodletting puncture were performed at HTWP twice a day for 2 days.The neurological function and cerebral edema were evaluated by modified neurological severity score(mNSS),cerebral water content,magnetic resonance imaging and hematoxylin and eosin staining.Cerebral blood flow was measured by laser speckles.The protein levels of aquaporin 4(AQP4),matrix metalloproteinases 9(MMP9)and mitogen-activated protein kinase pathway(MAPK)signaling were detected by immunofluorescence staining and Western blot.Results:Compared with TBI group,bloodletting puncture improved neurological function at 24 and 48 h,alleviated cerebral edema at 48 h,and reduced the permeability of BBB induced by TBI(all P<0.05).The AQP4 and MMP9 which would disrupt the integrity of BBB were downregulated by bloodletting puncture(P<0.05 or P<0.01).In addition,the extracellular signal-regulated kinase(ERK)and p38 signaling pathways were inhibited by bloodletting puncture(P<0.05).Conclusions:Bloodletting puncture at HTWP might play a significant role in protecting BBB through regulating the expressions of MMP9 and AQP4 as well as corresponding regulatory upstream ERK and p38 signaling pathways.Therefore,bloodletting puncture at HTWP may be a promising therapeutic strategy for TBI-induced cerebral edema. 相似文献
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抗冻蛋白(antifreeze protein,AFP)是一类结构多样的蛋白质,具有热滞效应(thermal hysteresis,TH,降低冰点而不改变熔点)和重结晶抑制效应(recrystalization inhabition,RI).通过非共价吸附抑制机制吸附到冰核表面,限制冰晶生长和抑制冰晶重结晶,从而保护有机体免受结冰引起的伤害.由于抗冻蛋白具有阻止冰晶生长而不破坏细胞的特点,因而利用抗冻蛋白在低温中长期保存各种细胞、组织和器官,特别在器官移植中可能具有很好的应用前景. 相似文献
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Song Wu Feng Wan Yong-shun Gao Zhe Zhang Hong Zhao Zhong-qi Cui Ji-yan Xie 《中国医学科学杂志(英文版)》2014,29(4):208-213
Objective To assess clinical effectiveness of using bilateral pectoralis major or plus rectus abdominis muscle flaps in treating deep sternal wound infection (DSWI) following median sternotomy. Methods Between January 2009 and December 2013, 19 patients with DSWI after median sternotomy for cardiac surgery were admitted to our hospital, including 14 males (73.7%) and 5 females (26.3%), aged 55±13 (18-78) years. According to the Pairolero classification of infected median sternotomies, 3 (15.8%) patients were type II, and the other 16 (84.2%) were type III. Surgical procedure consisted of adequate debridement of infected sternum, costal cartilage, granulation, steel wires, suture residues and other foreign substances. Sternal reconstruction used the bilateral pectoralis major or plus rectus abdominis muscle flaps to obliterate dead space. The drainage tubes were placed and connected to a negative pressure generator for adequate drainage. Results There were no intraoperative deaths. In 15 patients (78.9%), bilateral pectoral muscle flaps were mobilized sufficiently to cover and stabilize the defect created by wound debridement. 4 patients (21.0%) needed bilateral pectoral muscle flaps plus rectus abdominis muscle flaps because their pectoralis major muscle flaps could not reach the lowest portion of the wound. 2 patients (10.5%) presented with subcutaneous infection, and 3 patients (15.8%) had hematoma. They recovered following local debridement and medication. 17 patients (89.5%) were examined at follow-up 12 months later, all healed and having stable sternum. No patients showed infection recurrence during the follow-up period over 12 months. Conclusion DSWI following median sternotomy may be effectively managed with adequate debridement of infected tissues and reconstruction with bilateral pectoralis major muscle or plus rectus abdominis muscle flap transposition. 相似文献
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病人,女,81岁.40年前在工作时双上肢不慎被火烧伤,当年在大连化工厂医院烧伤科给予取自体腹部皮肤行前臂植皮术,术后植皮区域皮肤成活修复良好.4年前无明显诱因双侧前臂植皮区域肤色逐渐变深,无意中发现皮肤明显松弛,无不适感.近3个月自觉植皮区域疼痛不适,局部无红肿等,轻微牵拉皮肤时疼痛明显.自发病起4年皮损未见扩大.患者既往体健,否认家族有类似病患者. 相似文献
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1 临床资料 患者,男,23岁,因左下肢疼痛20 d来医院就诊.询问病史:患者2 d前在上级医院诊断为坐骨神经痛.既往健康,无药物过敏史.门诊当日给予维生素B1 100 mg,维生素B12 250靏肌注,用药约5 min时患者感到头晕、胸闷、呼吸困难、寒战,面色苍白,脉搏105次·min-1,血压80/50 mmHg,考虑为药物所致过敏性休克,立即给予平卧,氧气吸入,肾上腺素1 mg肌注,地塞米松10 mg静注,5 min后患者恢复正常. 相似文献
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多媒体教学手段在英语教学中运用的魅力 总被引:1,自引:0,他引:1
怎样运用多媒体优化英语课堂教学,已成为英语教学研究中的一个热点。多媒体可对学生的感官形成多种刺激,使教学内容活泼、生动,能够集中学生的注意力,激发学生的兴趣,使学生身心愉悦地掌握知识,避免让学生在紧张的心理状态下被动地学习,从而提高了教学效率。 相似文献
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图示教学法,是用一些线段或带箭头的线段把生化概念、规律联系起来的图形、图像或表格,从而揭示事物现象或本质的特征,激发学生思维,从而更好地掌握知识,加快教学进程的一种教学方法。其实质使科学知识形象化,抽象理论具体化,零碎知识系统化。现就图示的编制、教学步骤、应用和意义作一详尽说明,以飨同仁。 相似文献
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目的比较模拟教学与传统教学在诊断学问诊教学中的效果。方法在诊断学问诊教学中,选取具有可比性的2组教学班,进行模拟问诊教学与传统教学方法的对比研究。结果模拟问诊教学组的学生问诊技能考试成绩优于传统教学组,问诊内容得分两者无显著差异,该教学法深获学生的好评。结论模拟教学法有利于提高医学生的问诊技能及医患沟通能力,在医学教育领域中值得完善、推广。 相似文献
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方克伟 《中华医学教育探索杂志》2011,10(9):1084-1086
临床课双语教学是培养现代化高级医学人才的有效措施。建立“双语教学、目标导航、学用结合”的教学模式,结合现代化教学手段,培养了一批临床能力强、专业英语水平高的复合型高质量医学人才,值得深入探讨。 相似文献
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PBL教学模式在外科护理学实习教学中的应用 总被引:1,自引:0,他引:1
以问题为基础的学习教学是近年来国内外医学教育改革的热点.本文对该院2003级五年制护理系学生在外科学护理的临床实习教学中进行PBL教学改革的成效与经验进行总结,并就开展PBL教学改革存在的问题提出相应的对策. 相似文献
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生物化学教学中运用问题式教学法的几点体会 总被引:1,自引:1,他引:1
提问是教学中教师常用的一种教学方法,也是启发式教学常用的手段,是搞好教学、提高教学效率的重要手段。问题提得好可培养学生的学习兴趣。有了学习兴趣。学生就会积极、主动地学习,认真听课,积极恩考和回答教师提出的各种问题。在愉快、兴奋、积极、主动的状态下接受的知识也就牢固,学习效率自然会得到提高。笔者从事生物化学教学多年,深切感受到问题式教学法在生物化学教学中的重要作用。现将其体会总结如下。 相似文献
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模拟教学和培训已经越来越广泛地应用于医学教育中.各种逼真的模拟仪器为医学生技能操作练习提供了重要保证,并提高了教学的安全性,成为教学中不可缺少的工具之一.近年来,模拟教学在麻醉学教学中的应用日益广泛.本文介绍了麻醉学教学中常用的模型、模拟系统、模拟场所,总结了模拟教学在麻醉学临床培训、技能考核以及教学研究等方面的应用情况,并指出模拟系统的应用将成为麻醉学教学的必然趋势. 相似文献
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浅谈解剖生理学基础的插图教学 总被引:1,自引:1,他引:0
中专解剖生理学基础是国家规划教材(彭波主编,人卫出版社第一版),是在传统的人体解剖学和生理学2门必修主干课程基础上融合而成的重要医学基础教材。课本插图多,内容枯燥、抽象,增加了学习难度,而且学生对插图往往不够重视,有时习惯于只看文字内容,而忽视对插图的认识理解,造成识图能力较差,影响了对相关内容的理解记忆。插图作为教材内容的形象补充和延伸,也是教材的有机组成部分,与文字内容同样重要。所以插图的学习对学生学好这门课,在理解基础上掌握知识,使学生的综合素质得到提高都起到极大的促进作用。笔者结合自己的教学实践,对插图… 相似文献