基于活血、降脂活性丹参当归配比优化实验研究

目的:优化基于体外抗血小板聚集及降脂作用的丹参、当归最佳配伍剂量。方法:采用比浊法测定丹参、当归及不同剂量配比提取物体外抗ADP诱导血小板聚集率,计算IC50;采用高脂血症大鼠模型测定丹参、当归及不同剂量配比提取物对血脂的影响。结果:体外抗血小板聚集作用由强及弱依次为:丹参、当归剂量配比1∶31∶21∶1当归丹参。降低TC依次为:丹参、当归剂量配比1∶31∶2当归丹参1∶1。降低TG依次为:丹参、当归剂量配比1∶21∶31∶1丹参当归。降低LDL-C作用依次为:丹参、当归剂量比1∶3当归1∶2丹参1∶1。结论:丹参与当归配伍剂量比为1∶3时,抗血小板聚集能力和降脂作用活性最佳。     

丹参对人尿道狭窄成纤维细胞增殖的影响

目的:观察丹参对人尿道狭窄成纤维细胞(human urethral stricture fibroblasts,HUSF)增殖的影响,为预防及治疗尿道狭窄探寻新的思路及方法。方法:组织块法原代培养人尿道狭窄成纤维细胞,取第4-6代细胞冻存用于实验;将不同剂量的丹参含药血清加入HUSF分别培养24 h、48 h和72 h后,使用四唑盐比色法检测丹参对体外培养HUSF增殖的影响,利用增殖标志基因增生细胞核抗原(proliferating cell nuclear antigen,PCNA)免疫组织化学染色检测丹参对HUSF生长活性的影响,利用流式细胞术检测丹参对HUSF细胞周期的影响。结果:随丹参剂量增加和时间延长,体外培养HUSF的吸光度(OD值)逐步降低(P0.05)。HUSF的PCNA免疫组织化学染色的阳性率随丹参剂量增加而降低(P0.05)。流式细胞仪检测可见,丹参剂量越大,体外培养HUSF被阻断于G1期的细胞数量越多(P0.05)。结论:丹参可显著抑制HUSF的增殖,且抑制效果随丹参剂量及作用时间的增加而增强。     

丹参注射液对细胞基底样乳腺癌MDA-MB-231细胞的影响

目的:探讨丹参注射液对细胞基底样乳腺癌(basal-like)MDA-MB-231细胞增殖凋亡的影响。方法:实验分为对照组和5个不同剂量的丹参注射液组。MTT法检测增殖,流式细胞仪分析细胞周期,PI染色检测凋亡。结果:丹参注射液作用于MDA-MB-231细胞24 h,随着药物剂量的下降,其作用由促进增殖过渡为对增殖无影响再到抑制增殖,48 h、72 h分别表现出抑制增殖和促进增殖的作用。高、中剂量组诱导细胞凋亡;中低剂量组降低MDA-MB-231细胞G0/G1期DNA含量,增加S、G2-M期细胞DNA含量,可能促进了有丝分裂。结论:丹参注射液对basal-like型乳腺癌MDA-MB-231细胞增殖的影响与药物剂量相关,有时间依赖性。丹参诱导了MDA-MB-231细胞的凋亡,可能是P53基因调节的。     

镇眩饮治疗大鼠脑缺血的实验研究

比较镇眩饮、丹参、硝苯地平3种中,西药对大鼠脑缺血的治疗结果。采用Pulsinelli 4vo大鼠脑缺血模型,用~(122)Ⅰ-IMP示踪脑血流镇眩饮的有效剂量为0.3g/kg。最佳剂量为0.3g/kg。丹参的有效剂量为1g/kg,最佳剂量为3g/kg。硝苯地平的有效剂量为12mg/kg。镇眩饮在剂量上及对缺血脑的学习及记忆能力的影响方面,优于丹参;在改善脑缺血的范围及程度上优于硝苯地平。     

亚硒酸钠与丹参注射液抗血栓作用的正交设计研究

目的:研究亚硒酸钠与丹参注射液联合应用的抗血栓作用,寻求两药配伍使用的最佳剂量。方法:采用L9(34)的正交设计方法,考察药物对下腔静脉结扎法形成血栓的影响。结果:亚硒酸钠与丹参注射液最佳剂量组合为亚硒酸钠高剂量与丹参注射液中剂量。结论:亚硒酸钠高剂量(1×10-3 g/kg)与丹参注射液中剂量(3.75g/kg)伍用抗血栓效果最佳。     

丹参多酚酸盐对兔心肌再灌注损伤的保护作用

目的观察丹参多酚酸盐对离体家兔缺血再灌注损伤的保护作用。方法制备全心缺血-再灌注模型,观察丹参多酚酸盐三个剂量对血液动力学、心肌酶学和心肌细胞结构的影响。结果丹参多酚酸盐呈剂量依赖改善血液动力学;降低流出液中CK-MB、LDH、MDA含量;与缺血组相比,细胞超微结构损伤轻微。结论丹参多酚酸对家兔心肌缺血再灌注损伤具有保护作用。     

高剂量丹参、川芎对Lewis肺癌小鼠肿瘤的影响及机制研究

目的探究高剂量活血化瘀药丹参、川芎对Lewis肺癌小鼠肿瘤侵袭转移的影响及其可能作用的机制。方法采用腋下接种Lewis肺癌细胞悬液的方法制备Lewis肺癌小鼠模型30只,随机分为模型组、顺铂组、高剂量丹参组、高剂量川芎组、丹参联合顺铂组和川芎联合顺铂组,每组5只。顺铂组、丹参联合顺铂组和川芎联合顺铂组均给予顺铂4 mg/kg腹腔注射,高剂量丹参组、丹参联合顺铂组以2. 16 g/kg剂量丹参灌胃,高剂量川芎组、川芎联合顺铂组以3. 6 g/kg剂量川芎灌胃,模型组给予等体积生理盐水腹腔注射和灌胃。灌胃1次/d,腹腔注射于造模后第1、3、5天进行。每2 d记录1次小鼠体重,并于治疗后第14天将小鼠断颈处死取材,称取肿瘤质量,计算抑瘤率;采用Western-Blot法检测肿瘤组织内Twist和血管内皮生长因子(VEGF)的表达。结果各组小鼠体重,模型组、高剂量丹参组和高剂量川芎组顺铂组、丹参联合顺铂组和川芎联合顺铂组(P 0. 05),高剂量丹参组、高剂量川芎组和模型组比较差异无统计学意义。计算抑瘤率,发现中药对肿瘤生长并没有抑制作用,反而具有促进作用。肿瘤组织免疫印迹结果显示高剂量川芎组肿瘤组织Twist蛋白表达高于模型组(P 0. 05),高剂量丹参组肿瘤组织VEGF-A蛋白表达高于模型组(P 0. 05)。结论高剂量活血化瘀中药川芎、丹参对Lewis肺癌小鼠肿瘤侵袭转移具有促进作用,其机制可能与调控Twist和VEGF-A蛋白的表达有关。     

参芎葡萄糖注射液及其丹参组分对小鼠核受体表达的调控作用

目的 考察参芎葡萄糖注射液(SGI)及其丹参组分(SM)对小鼠肝细胞中芳香烃受体(AhR)、肝细胞核因子4α(HNF-4α)、孕烷X受体(PXR)和过氧化物酶体增殖物激活受体α(PPARα)mRNA表达的影响.方法 采用随机数表法将40只雄性小鼠分为8组,每组5只,分别为空白组、阳性组(苯巴比妥)、SGI低剂量组(以丹参素计2.6 mg·kg-1·d-1)、SGI中剂量组(以丹参素计3.9 mg·kg-1·d-1)、SGI高剂量组(以丹参素计5.2 mg·kg-1·d-1)、SM低剂量组(以丹参素计2.6 mg·kg-1·d-1)、SM中剂量组(以丹参素计3.9 mg·kg-1·d-1)、SM高剂量组(以丹参素计5.2 mg·kg-1·d-1),连续给药7 d.提取各组小鼠肝脏组织中的RNA,检测相关核受体mRNA的变化.结果 与空白组相比,SGI高剂量组可以上调AhR mRNA的水平(P<0.05),上调倍数为1.7倍,但是SGI对PPARα、PXR、HNF-4α的mRNA水平没有显著性影响(P>0.05);SM对AhR、PPARα、PXR和HNF-4α的mRNA表达均无显著性影响(P>0.05).结论 高剂量的参芎葡萄糖注射液能够影响AhR mRNA的表达,但这种影响并不是由其丹参组分导致的.     

丹参素增强肝细胞生长及其在药物性肝细胞损伤中的保护作用

以体外培养的小鼠肝细胞为模型，采用四甲基偶氮唑盐（ＭＴＴ）比色法，观察了不同剂量丹参素对肝细胞增殖的影响及其对Ｄ－半乳糖胺（ＧａｌＮ）致肝细胞损伤的保护作用。结果表明：丹参素能直接刺激肝细胞增殖，而且二者之间具有一定的剂量依赖关系；丹参素对ＧａｌＮ引起的肝细胞损伤和坏死也具有保护作用。     

丹参水提取物对小鼠耐缺氧作用的影响

目的观察丹参水提取物对小鼠耐缺氧作用的影响。方法使用水提醇沉法制备丹参水提取物。将小鼠分为空白对照组、红景天对照组（阳性对照组）和丹参水提取物低、中、高剂量组,通过常压密闭缺氧实验、KCN中毒缺氧实验和急性脑缺血性缺氧实验观察丹参水提取物对缺氧小鼠的保护作用及其对小鼠外周血象的影响。结果与空白对照组比较,丹参水提取物组和红景天对照组可以明显延长小鼠常压密闭缺氧情况下的存活时间,并且可以改善小鼠KCN中毒状况,避免小鼠死亡。丹参水提取物还可以明显增加小鼠断头后的呼吸次数（P〈0.05）。丹参水提取物的中、高剂量组在3种缺氧情况下的抗缺氧效果与红景天对照组相当（P〉0.05）。和空白对照组比较,丹参水提取物组和红景天对照组还可以显著提高小鼠外周血中红细胞和血红蛋白的含量（P〈0.05或P〈0.01）,丹参水提取物高剂量组和红景天对照组还显著提高了小鼠外周血中白细胞的含量（P〈0.01）。结论丹参水提物可以有效地提高小鼠耐缺氧的能力。     

Effect of radix Salviae miltiorrhizae extract injection on survival of allogeneic heart transplantation

The effect of Radix Salviae Miltiorrhizae (RSM), a commonly used herbal blood circulation invigorator for the treatment of blood stasis in traditional Chinese medicine, on the duration of allograft survival following heterotopic heart transplantation in experimental animals was observed. The results in three heart transplantation models--auricular free graft in mice, abdominal graft in rats and cervical graft in rabbits--suggested that RSM injection in an appropriate dosage prolonged the survival time of cardiac allograft. The herb showed no significant toxicity. It was also found that RSM injection had a synergic effect with corticosteroids against graft rejection.     

The Inhibitory Effect of Radix Salviae Miltiorrhizae on Hypoxic Structural Remodeling of Intra－acinarPulmonary Arteries

(席思川)(车东媛)(张婉蓉)ＴｈｅＩｎｈｉｂｉｔｏｒｙＥｆｆｅｃｔｏｆＲａｄｉｘＳａｌｖｉａｅＭｉｌｔｉｏｒｒｈｉｚａｅｏｎＨｙｐｏｘｉｃＳｔｒｕｃｔｕｒａｌＲｅｍｏｄｅｌｉｎｇｏｆＩｎｔｒａ－ａｃｉｎａｒＰｕｌｍｏｎａｒｙＡｒｔｅｒｉｅｓ￥ＸＩＳｉ－ｃ...     

丹参对大鼠急性胃粘膜损伤后LPO、SOD及GSH－Px含量或活性的影响

本文观察了丹参对实验性急性胃粘膜损伤大鼠胃粘膜组织过氧化脂质（ＬＰＯ）含量及超氧化物歧化酶（ＳＯＤ）和谷胱甘肽过氧化物酶（ＧＳＨ－Ｐｘ）活性的影响。结果为丹参组胃粘膜ＬＰＯ含量显著低于乙醇组（Ｐ＜０．０１），而ＳＯＤ和ＧＳＨ－Ｐｘ活性高于乙醇组（Ｐ＜０．０１，Ｐ＜０．０５）。经积差相关分析，丹参组和乙醇组胃粘膜ＬＰＯ含量与胃粘膜损伤率呈正相关。提示丹参的胃粘膜保护作用与清除氧自由基、抑制脂质过氧化反应和提高组织氧化能力有关。     

The preventive effect of Radix Salciae Miltiorrhizae on monocrotaline-induced pulmonary hypertension in rats

Summary The preventive effect of Radix Salciae Miltiorrhizae (RSM) on pulmonary hypertension (PHT) and right ventricular hypertrophy (RVH) induced by monocrotaline (MCT) in rats was observed with the methods of measuring the right ventricular systolic pressure (PVSP), the ratio of the right ventricle to the left ventricle plus interventricular septum RV/(LV+ S) and the pulmonary small artery morphological analysis. The results show that RSM can reduce the PHT, and prevent the RVH and the increase of the medial thickness of pulmonary small arteries. It can also prevent the endothelial cell injury produced by MCT. The pharmacological effects of RSM on pulmonary circulation were also discussed.     

Radix Salviae miltiorrhizae protects rat hippocampal neuron in culture from anoxic damage.

Radix Salviae Miltiorrhizae (RSM), a well-known traditional Chinese medicinal herb, has been used to improve blood circulation and resolve blood stasis. We have previously found that RSM has neuroprotective effect on ischemia and/or ischemia-reperfusion rats. The purpose of this study was to obtain further information on the mechanism of the RSM-induced neuroprotection and to examine the neuroprotective effect on neurons exposed to anoxia. The effect of RSM on anoxic damage in cultured hippocampal neurons of neonatal rat was investigated by using morphological changes and heat shock protein 70 kD (HSP70) expression as indicators. RSM given 0.5 h before 2 h-anoxia followed by 48 hours reoxygenation could significantly increase survival rate of hippocampal neurons and number of HSP70 positive cells. The results suggest that RSM has a direct neuroprotective effects on anoxic damage in hippocampal neurons.     

Effect of Radix Salviae Miltiorrhizae on Production of Lung Oxygen Free Radical Products during Cardiopulmonary Bypass Operation

Ｅｆｆｅｃｔ　ｏｆ　Ｒａｄｉｘ　Ｓａｌｖｉａｅ　Ｍｉｌｔｉｏｒｒｈｉｚａｅ　ｏｎ　Ｐｒｏｄｕｃｔｉｏｎ　ｏｆ　Ｌｕｎｇ　Ｏｘｙｇｅｎ　Ｆｒｅｅ　Ｒａｄｉｃａｌ　Ｐｒｏｄｕｃｔｓ　ｄｕｒｉｎｇ　Ｃａｒｄｉｏｐｕｌｍｏｎａｒｙ　Ｂｙｐａｓｓ　Ｏｐｅｒａｔ...     

肢体缺血再灌注致肠损伤及丹参的保护作用

目的：通过动物实验观察丹参作肢体缺血再灌注所致肠损伤的保护作用。方法：８０只Ｗｉｓｔａｒ大鼠随机分成正常对照组，缺血３ｈ和缺血３ｈ用丹参３个组。实验组于缺血再灌注１，２，３和２４ｈ取材光镜对比观察和组织学评估。结果：肢体缺血和再灌注后，实验组肠粘膜固有层毛细血管扩张、充血、大量多核白细胞浸润。大肠和小肠粘膜厚度和隐窝深度明显减少（Ｐ〈０．０１），小肠绒毛高度相对增加（Ｐ〈０．０１）。隐窝细胞有变性坏死，用丹参组的病变明显轻于未用丹参组。结论：丹参能有效地减轻肢体缺血再灌注所致的肠损伤。     

Effect of radix Salviae miltiorrhizae on the gene expression of nitric oxide synthase in ischemic rat brains.

The effect of Radix Salviae Miltiorrhizae (RSM) on the gene expression of nitric oxide synthase (NOS) in rat brains during ischemia was studied with in situ hybridization and the results were analyzed with IBAS 2000 Image Analysis System. It was found that NOS gene expression of cerebral cortex and caudate-putamen was markedly increased in 24 hours in ischemia group (P < 0.01). In RSM-treated rats, although the NOS gene expression of ischemic side was also increased as compared with contralateral cortex and caudate-putamen, it was significantly lower in RSM-treated rats than those of the controls (P < 0.05, P < 0.01). The present study indicates that RSM can partly inhibit NOS gene expression of cerebral cortex and caudate-putamen during ischemia. This may be one of the protective mechanisms of RSM on cerebral ischemia.     

丹参在体外循环中对肺的防护作用

目的探讨体外循环前应用丹参预防体外循环时肺的损伤。方法３０例心脏瓣膜置换手术患者，随机分为对照组和丹参组，各１５例。于体外循环前及心脏复跳后１０ｍｉｎ、４５ｍｉｎ、２ｈ、６ｈ检测左、右心房血白细胞数和过氧化脂质浓度，并于体外循环前、后取肺组织作活检。结果丹参能明显抑制肺再灌注早期白细胞肺内聚集，减少肺内氧自由基的产生，体外循环后肺组织病理检查显示用药组未见明显白细胞聚集、渗出，肺组织损伤较对照组减轻。结论丹参对体外循环肺损伤有防护作用。     

ET-1 gene expression of rat brain during ischemia and reperfusion and the protective effect of radix Salviae miltiorrhizae.

Endothelin-1 (ET-1) gene expression of rat brain during ischemia and reperfusion as well as the effect of Radix Salviae Miltiorrhizae (RSM) were studied with in situ hybridization. It was found that ET-1 gene expression of cerebral cortex and caudate-putamen was markedly increased both in 24 hours of ischemia and 24 hours of reperfusion groups (P < 0.01, P < 0.05). In RSM-treated rats, although the ET-1 gene expressions of ischemia and reperfusion sides were also increased as compared with contralateral cortex and caudate-putamen, they were significantly lower in RSM-treated rats than those of controls (P < 0.05, P < 0.01 respectively). The present study indicated that RSM can partly inhibit ET-1 gene expression of cerebral cortex and caudate-putamen during ischemia and reperfusion. This may be one of the protective mechanisms of RSM on cerebral ischemia and reperfusion.