他莫昔芬对结肠癌细胞株生长的抑制作用

目的　研究他莫昔芬 (tamoxifen ,TMX)对结肠癌细胞株生长的抑制作用。方法　选用Caco 2和LoVo两种结肠癌细胞株 ,采用酸性磷酸酶法测定 1× 10 -4～ 1× 10 -9mol/LTMX并联合应用 5 氟尿嘧啶 (5 FU)时对细胞生长的抑制作用。以流式细胞仪测定细胞周期的改变及凋亡。结果　TMX对两种细胞株均有一定的抑制作用 ,且TMX对细胞生长抑制作用呈剂量依赖性。TMX +5 FU有协同作用 ,其作用较单药强。流式细胞仪检测表明与对照组相比 ,TMX组、5 FU组及联合用药组对细胞株均有不同程度的生长抑制作用。尤其是联合用药组 ,TMX可加强 5 FU的作用 ,强烈地诱导细胞凋亡 ,凋亡细胞比例为 33.2 6 % (LoVo细胞株 )和 4 5 .87% (Caco 2细胞株 )。结论　TMX可抑制结肠癌细胞株生长 ,并可加强 5 FU的作用。其机制可能是通过雌激素受体 (ER) β来诱导肿瘤细胞凋亡     

ERβ在结肠癌细胞株的表达及他莫昔芬对结肠癌细胞株体外抑制实验

目的：探讨ERβ在结肠癌细胞株Lovo、HT-29的表达和他莫昔（tamoxifen,TAM）对结肠癌细胞株抑制作用.方法：体外培养人结肠癌细胞株Lovo、HT-29,用细胞爬片免疫组化、RT-PCR、Western blot方法检测ER α、ERβ表达,用MTT法检测不同浓度的17β-E2、TAM、5-Fu、TAM＋5-Fu、TAM对结肠癌细胞株的生长影响作用.流式细胞仪（FCM）测定细胞凋亡率.结果：①在结肠癌细胞株Lovo、HT29中稳定表达ERβ而不表达ER α ②TAM、5-Fu能抑制Lovo、HT29细胞株的增殖 ③TAM协同5-FU对Lovo、HT29细胞株有促使细胞凋亡的作用.结论：①结肠癌细胞株Lovo表达ERβ而不表达ER α ②TAM以ERβ为靶点抑制Lovo细胞株的增殖 ③TAM协同5-FU抑制Lovo细胞株的增殖,TAM协同5-FU对Lovo、HT29细胞株促细胞凋亡的作用.     

西咪替丁对人结肠癌细胞株LoVo生长及凋亡的影响

目的:探讨西咪替丁对体外培养的人结肠癌细胞株LoVo的抑制和诱导细胞凋亡的作用.方法:采用MTT比色法、流式细胞术、形态学观察和TUNEL法检测不同浓度的西咪替丁溶液处理LoVo细胞后的生长状况、形态学、分子生物学改变.结果:西咪替丁可抑制LoVo细胞的增殖,且有剂量依赖性的趋势:1×10-2mol/L西咪替丁处理LoVo细胞48h后,在光镜、电镜检测中,可见典型的凋亡细胞;流式细胞术检测凋亡率为32.7%,bcl-2相关蛋白表达率为36.74%;TUNEL法进一步从分子生物学角度证实凋亡的发生.结论:西咪替丁对结肠癌细胞LoVo有抑制作用,主要机制为诱导其发生凋亡,凋亡发生的原因与西咪替丁可下调bcl-2蛋白的表达有关.     

蛇床子素对小鼠胚胎长骨雌激素受体α、β蛋白表达的影响

【目的】研究蛇床子素对小鼠胚胎长骨雌激素受体α（estrogen receptorα,ERα）和骨雌激素受体β（estrogen receptorβ,ERβ）蛋白表达的影响,探讨其作用于骨组织的调控机制。【方法】取16 d胎龄雌性小鼠前肢尺骨,置BGJb培养基中孵育,经不同浓度蛇床子素（1×10^-4、1×10^-5、1×10^-6、1×10^-7mol/L）和雌酚酮（1×10^-6mol/L）作用48 h后,采用免疫组织化学染色方法观察ERα、ERβ在小鼠尺骨骺板静止区、增殖区和肥大区中的定位和表达情况,并通过图像分析系统测定骺板各区免疫反应阳性细胞数和阳性细胞面积。【结果】与对照组相比较,各浓度蛇床子素和雌酚酮组均能显著上调软骨骺板静止区、增殖区和肥大区ERα、ERβ蛋白的表达水平（P〈0.01）。在1×10^-7-1×10^-5mol/L区间内,随着蛇床子素浓度的升高,各区ERα、ERβ蛋白的表达水平显著升高（P〈0.01）。除1×10^-5mol/L组外,其它各浓度蛇床子素对ERα、ERβ蛋白的调控作用明显弱于雌酚酮组（P〈0.05）,1×10^-5mol/L蛇床子素组作用与雌酚酮组无统计学差异。【结论】蛇床子素对骨组织的作用可能与其上调长骨骺板中ERα及ERβ表达有关。     

三苯氧胺联合5-氟尿嘧啶对人结肠癌细胞HT-29的抑制作用

目的 探讨三苯氧胺(tamoxifen,TAM)联合5-氟尿嘧啶(5-FU)对人结肠癌细胞株HT-29的增殖、凋亡及细胞迁移的影响.方法 采用MTT法、流式细胞仪分别检测不同浓度(12.5、25、50 μmol/L)5-FU及其联合TAM对HT-29细胞增殖的抑制作用、细胞凋亡的诱导作用,应用细胞划痕实验检测TAM(1×10-7、1×10-6、1×10-5、1×10-4mol/L)、5-FU及其联合TAM对细胞迁移的抑制作用.结果 5-FU及其联合TAM均可提高HT-29细胞增殖抑制率,单用5-FU的抑制率分别为(50.8±8.9)%和(62.8±8.8)%和(69.7±9)%,12.5 μmol/L 5-FU联合低浓度TAM(1×10-7、1×10-6 mol/L)的抑制率分别为(82.9±2.9)%和(84.0±1.6)%,其作用呈剂量依赖关系;高浓度(1×10-4mol/L)TAM联合12.5 μmol/L 5-FU表现出明显的促细胞凋亡作用[凋亡率为(95.7±4.2)%];划痕实验证实经1×10-6和1×10-5mol/L TAM及单用5-FU处理HT-29细胞72 h后均可明显地抑制细胞的迁移,但联合用药未显示出较TAM单药更明显的抑制作用.结论 TAM和5-FU均具有抑制细胞增殖、迁移和促进细胞凋亡的作用,TAM亦可增加结肠癌细胞株HT-29对5-FU的敏感性.     

抑癌基因PTEN对人结肠癌LoVo细胞生长的影响

目的探讨PTEN基因转染对结肠癌LoVo细胞的抑制作用.方法将人野生型及突变型PTEN cDNA通过脂质体导入结肠癌细胞LoVo中,并用RT-PCR、流式细胞术检测细胞的PTEN mRNA和蛋白质表达.用流式细胞仪和MTT法分析5-Fu对转染前后LoVo细胞抑制周期和诱导凋亡的能力.结果转染野生型PTEN基因可明显增强结肠癌LoVo细胞的PTEN表达,5-Fu对转染后的LoVo细胞抑制细胞周期和诱导凋亡的作用明显增强.突变型在结肠癌细胞LoVo中有表达但无明显抑制作用.结论转染的PTEN基因可显著上调结肠癌LoVo细胞的PTEN表达,促进细胞凋亡;联合应用PTEN基因转染和5-Fu可获得协同的细胞毒作用.     

抑癌基因PTEN对人结肠癌LoVo细胞生长的影响

目的:探讨PTEN基因转染对结肠癌LoVo细胞的抑制作用。方法:将人野生型及突变型PTENcDNA通过脂质体导入结肠癌细胞LoVo中,并用RT-PCR、流式细胞术检测细胞的PTENmRNA和蛋白质表达。用流式细胞仪和MTT法分析5-Fu对转染前后LoVo细胞抑制周期和诱导凋亡的能力。结果:转染野生型PTEN基因可明显增强结肠癌LoVo细胞的PTEN表达,5-Fu对转染后的LoVo细胞抑制细胞周期和诱导凋亡的作用明显增强。突变型在结肠癌细胞LoVo中有表达但无明显抑制作用。结论:转染的PTEN基因可显著上调结肠癌LoVo细胞的PTEN表达,促进细胞凋亡;联合应用PTEN基因转染和5-Fu可获得协同的细胞毒作用。     

4-羟基他莫昔芬对体外培养前列腺平滑肌细胞的作用

目的 研究4-羟基他莫昔芬(OHT)对原代培养的前列腺平滑肌细胞增殖与凋亡以及雌、雄激素受体表达的影响.方法 以不同浓度的雌二醇(E2)、乙烯雌酚(DES)和OHT分别作用于原代培养的前列腺平滑肌细胞,采用流式细胞术检测增殖和凋亡,免疫细胞化学检测雌、雄激素受体表达.结果 DES和E2在一定浓度范围内起促增殖作用(均P<0.05),但未发现明显的浓度相关性(DES:r=-0.101,P=0.328;E2:r=-0.115,P=0.188).OHT浓度在1×10-8moL/L时,前列腺平滑肌细胞增殖率为5.04%±0.65%,随着浓度由1×10-6mol/L升高为1×10-5mol/L,细胞增殖率由2 79%±0 54%下降为0.93%±0.40%.在更高浓度下显示与浓度相关的促凋亡作用(5.83%±0.78%、13.70%±0.71%、19.53%±0.90%),以上作用不能被相同甚至更高浓度的E2所逆转.OHT对雌、雄激素受体表达的影响与雌激素相近.结论 OHT在一定浓度范围内对原代培养的前列腺平滑肌细胞具有明显的抑制增殖和促凋亡作用,且该作用可能不依赖于雌激素受体途径.口服他莫昔芬后OHT血药浓度较低和OHT上调雄激素受体表达可能是他莫昔芬治疗前列腺增生效果不佳的原因.     

3,6-二羟基黄酮对多种肿瘤细胞的增殖抑制及凋亡诱导作用

目的 观察3,6-二羟基黄酮对乳腺癌细胞株MCF-7(雌激素受体阳性ER+)、MDA-MB-231(雌激素受体阴性ER-)、直肠癌细胞株LoVo、DLD-1和前列腺癌细胞株PC3增殖和凋亡的影响.方法 MTT法检测3,6-二羟基黄酮对不同肿瘤细胞生长存活的影响,计算半数抑制浓度(IC50);DAPI染色观察3,6-二羟基黄酮对肿瘤细胞生长状态及形态的影响,Annexin V/PI双染流式细胞仪检测细胞凋亡变化.结果 增殖检测结果表明,3,6-二羟基黄酮对于MCF-7、MDA-MB-231、LoVo、DLD-1和PC3细胞增殖均表现出显著抑制作用,48 h IC50值分别为16.1、15.7、29.8、12.8和17.8μmol/L;细胞形态观察和凋亡检测结果表明,10 μmol/L的3,6-二羟基黄酮作用24 h,5种肿瘤细胞的生长状态和形态均发生显著变化,大量细胞表现出凋亡或死亡细胞的形态特征,细胞凋亡率均显著升高.结论 3,6-二羟基黄酮具有显著的抗肿瘤效应,对于多种肿瘤细胞具有很强的增殖抑制和凋亡诱导作用.     

三氧化二砷通过诱导结肠癌LoVo细胞凋亡和坏死抑制其生长

目的 探讨三氧化二砷（As2O3)对结肠癌细胞系LoVo细胞生长的影响及可能机制。方法 通过肿瘤细胞记数,观察As2O3对LoVo细胞生长的影响。应用光镜、电镜、流式细胞仪以及生化分析仪,观察细胞形态学、凋亡细胞峰并测定培养上清液中乳酸脱氢酶（LDH）含量。结果 As2O3明显抑制LoVo细胞生长,并呈时间和剂量依赖关系（P＜0．05或P＜0．01）。光镜及电镜下可见典型的细胞凋亡和坏死形态表现。流式细胞仪分析显示：在G1期前出现亚二倍体的凋亡细胞峰。生化分析测定发现：As2O3浓度为2．0μmol/L时,培养上清液中LDH含量增加（P＜0．05或P＜0．01）。结论 As2O3通过诱导LoVo细胞凋亡和坏死抑制其生长。     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

Acupuncture Treatment of Vomiting

CASE HISTORY A female patient, 46 years old, head of the foreign affairs department of a certain university in Beijing, paid her first visit on October 9, 2006, with the chief complaint of vomiting for one month. She got vomiting after meals in early September. Before that, she had discomfortable sensation in the stomach due to angry with others, but she didn't pay much attention. Later, it developed into vomiting after eating. After the vomiting, the discomfort would be relieved, but with slight hypodynamia. She was once diagnosed as having 'neurogenic vomiting'. Having taken some western and Chinese drugs, the above symptoms were a little bit improved, but she would have nausea upon eating and with regurgitation. Because of the fear for vomiting, she did not dare to have food intake, with body weight reduction of 6 kilos in one month.     

Qiu Baoguo''''s Experience in Treating Consumptive Syndromes after Radio-Chemotherapies

Radiotherapy and chemotherapy are the important modern medical therapies for malignant tumors,yet they can also bring about serious local and systemic toxic side reactions so to decrease the patient;'s life quality,manifested by a series of consumptive symptoms.Having engaged in the combined work of Chinese and western medicine for nearly 50 years,the research fellow Qiu Baoguo in Henan Provincial Academy of TCM has developed his unique views on the TCM study of consumptive syndromes.The author of this essay had once the fortune tO follow Dr.Qiu in clinic,and specially would like to introduce in the following Dr.Qiu's experience in treating consumptive syndromes after radio-chemotherapies for patients with malignant tumor.     

Insomnia Due to Deficiency of Both the Heart and Spleen Treated by Acupuncture-Moxibustion and Chinese Tuina

OBJECTIVE: To observe therapeutic effects of the comprehensive therapy of acupuncture-moxibustion and Chinese Tuina for treatment of insomnia due to deficiency of both the heart and spleen. METHODS: 92 cases were divided randomly into the treatment group (treated by acupuncture-moxibustion and Chinese Tuina) and the control group (treated by acupuncture-moxibustion). RESULTS: The therapeutic effect of the treatment group was obviously superior to that of the control group (the CHI2 test showed P < 0.01). CONCLUSIONS: The comprehensive therapy of acupuncture-moxibustion and Chinese Tuina can give marked therapeutic effects for treatment of insomnia due to deficiency of both the heart and spleen.     

Analysis of CK-19,PDX-1,Nestin,Ngn3 in different donor islet purity in rats

Objective: To investigate if there are the CK-19, PDX-1, Nestin, Ngn3 positive cells in the donor islets of different purity in rats. Methods: Thirty male adult SD rats were randomly divided into 3 groups. Islets were isolated using digestion by ductal injection of collagenase. Group Ⅰ (n=10): Separating cell preparations were not purified, Group Ⅱ(n=10): Islet sediment was purified with 25% Ficoll400 ,Group Ⅲ (n=10): Islet sediment was purified with 25% and 11% Ficoll-400. The levels of protein of CK-19, PDX-1, Nestin and Ngn3 were detected by immunohistochemistry and the mRNA of CK-19, PDX-1, Nestin, Ngn3 was amplified by RT-PCR. Results: After two different purification methods applied, three islet preparations of different purities were obtained. The difference of islet purity was significant among various groups (P＜0.05). Compared with group Ⅱ and group Ⅲ,the protein and mRNA of CK-19, PDX-1, Nestin,Ngn3 were both higher in group Ⅰ; group Ⅲ was poorly expressed. Conclusions: The three different islet purity donor islet have different CK-19, PDX-1, Nestin, Ngn3 positive cells within them, indicating that there are some islet stem cells in the purified donor islet.     

Dr.Zhang Ren''''s Experience in the Acupuncture Treatment of Different Diseases with the Same Therapeutic Principle

Dr.Zhang Ren,the chief physician,is the chairman of Shanghai Acupuncture and Moxibustion Association.Having been engaged in medicine for about 40 years,he is experienced in treating various intractable diseases.In his long years of clinical practice,he advocates taking the TCM differentiation as the basis to seek for the acupuncture method for treatment of modern intractable diseases.The author of this essay had the fortune to follow Dr.Zhang in study.The following is a summary of Dr.Zhang's experience in the acupuncture treatment for different intractable diseases with the same therapeutic principle.     

Luo Lingjie''''s Experience in Treating Chronic Nephropathy

In treating chronic nephropathy,Luo Lingjie,a chief physician,pays attention to regulating the balance between yin and yang,treating infection if present,and removing pathogenic factors.He prescribes gentle drugs and uses carefully strongly warming-tonifying ones,emphasizes the importance of persuading the patient to persist in treatment with medication and nurse one's health for recuperation,and is good at combined use of TCM and western medicine therapy and brings the merits of various therapies into full play,with obvious theraoeutic effects.     

Acupuncture Combined with Spinal Tui Na for Treatment of Primary Dysmenorrhea in 30 Cases

Objective: To observe the therapeutic effects in acupunture treatment of primary dysmenorrhea combined with spinal Tui Na, and study its mechanism. Methods: Thirty cases of the treatment group were treated by acupuncture combined with spinal Tui Na, and thirty cases in the control group were treated by routine acupuncture. Results: The total effective rate was 93.3% in the treatment group, and 73.3% in the control group, with a significant difference between the two groups (P<0.05). Conclusions: Acupuncture combined with spinal Tui Na has good prospects for treatment of primary dysmenorrhea.