扶正益气中药对脾虚大鼠脑内IL-6Rα水平及其基因表达的影响

目的研究脾虚大鼠脑内白介素-6受体α（interleukin-6receptora,IL-6Rα）水平及其mRNA表达的变化,以及扶正益气中药的干预作用。方法将40只SPF级雄性Wistar大鼠分为正常组、模型组、治疗1组（四君子汤治疗）、治疗2组（玉屏风散治疗）。采用耗气破气、饮食失节、劳倦过度结合的方法,复制脾虚动物模型;采用免疫组织化学法测定大鼠脑内IL-6Rα水平,原位杂交法测定大鼠脑内IL-6RamRNA表达的变化。结果与正常组比较,模型组大鼠海马CAI区和下丘脑腹侧核IL-6Rα水平及IL-6RamRNA表达水平显著升高（P〈0．01）;与模型组比较,治疗1组和治疗2组IL-6Rα水平及IL-6RαmRNA表达水平显著降低（P〈0．01）;治疗1组与治疗2组IL-6Ra水平及IL-6RamRNA表达水平比较,差异无显著性。结论脾虚可导致IL-6Rα水平及其mRNA表达水平异常,扶正益气类中药四君子汤、玉屏风散可以通过调节IL-6Rα水平及其mRNA表达水平,改善机体防御功能。     

隐型孢子虫病患者细胞免疫功能及细胞因子网络的实验研究

目的:为了探讨细胞免疫功能及细胞因子在隐型孢子虫感染中的作用.方法:本文采用免疫分析技术对29例隐型孢子虫病患者外周血T细胞亚群,红细胞免疫功能及血清中IL-2(白细胞介素-2)、IL-6(白细胞介素-6)、sIL-2R(可溶性IL-2受体)、TNF-α(肿瘤坏死因子-α)及sICAM-1(可溶性细胞间粘附分子-1)等细胞因子水平进行测定.结果:患者外周血CD 4T细胞、CD4/CD8比值和红细胞表面C3b受体花环率(C3bRR)显著低于正常对照组(P＜0.01),血清中IL-2、sIL-2R、IL-6、TNF-α及sICAM-1水平则显著高于正常对照(P＜0.01).结论:隐孢子虫感染可致机体细胞免疫功能降低,细胞因子网络失衡.     

慢性丙型肝炎患者的细胞免疫功能

目的探讨慢性丙型肝炎患者的细胞免疫功能及细胞因子生成量变化。方法采用免疫技术对31例慢性丙型肝炎患者外周血T细胞亚群,红细胞免疫功能及血清中转化生长因子-β（TGF-β）、白细胞介素-2（IL-2）、可溶性白细胞介素-2受体（sIL-2R）、肿瘤坏死因子-α（TNF-α）及可溶性细胞间粘附分子-1（sICAM-1）等细胞因子水平进行检测。结果慢性丙型肝炎患者外周血CD4＋T细胞,CD4/CD8比值及红细胞表面C3b受体花环率（RBC-C3bRR）显著低于正常对照（P〈0.01）;CD8＋T细胞及血清中TGF-β、IL-2、sIL-2R、IL-6、TNF-α及sICAM-1则显著高于正常对照组（P〈0.01）。结论丙型肝炎病毒的感染可致患者细胞免疫功能紊乱,细胞因子过量表达。     

益气健脾止泻汤对脾虚型溃疡性结肠炎大鼠TNF-α和 IL-8的影响

目的：探讨益气健脾止泻汤对脾虚型溃疡性结肠炎大鼠模型TNF-α和IL-8的干预调节作用。方法建立脾虚型溃疡性结肠炎大鼠模型,观察益气健脾止泻汤对溃疡性结肠炎大鼠血清TNF-α和IL-8表达水平的影响。结果与模型组比较,益气健脾止泻汤可显著降低模型大鼠血清中TNF-α、IL-8水平（P＜0．01）。结论益气健脾止泻汤治疗脾虚型溃疡性结肠炎的作用机理与下调促炎因子TNF-α、IL-8的表达有关。     

外周血T淋巴细胞激活与哮喘发病的关系

目的：探讨外周血T淋巴细胞激活释放的细胞因子在哮喘发病中的作用。方法：采用酶联免疫法检测30例哮喘患者及20名健康人血清可溶性白细胞介素-2受体（sIL-2R)、白细胞介素（IL）-4、IL-5、肿瘤坏死因子（TNF）-α水平。结果：哮喘患者血清sIL-2R、IL-4、IL-5及TNF-α浓度均明显高于正常对照组。经糖皮质激素治疗后sIL-2R、IL-4、IL-5和TNF-α浓度显著下降。结论：T淋巴细胞激活释放的细胞因子在哮喘发病中起重要作用,糖皮质激素抗气道炎症作用,可能是通过抑制T淋巴细胞活化,减少细胞因子释放来实现的。     

儿童腺病毒肺炎免疫功能变化及免疫治疗的临床研究

目的 探究儿童腺病毒肺炎免疫功能变化及免疫治疗对其影响。方法 回顾性选取2020年12月至2022年3月江西省儿童医院收治的26例腺病毒肺炎患儿作为病例组,选取同期就诊的25例上呼吸道感染患儿作为对照组。观察治疗前后病例组患儿外周血T淋巴细胞亚群（CD16+CD56+、CD4、CD8、CD19、CD4/CD8）、免疫球蛋白（immunoglobulin,Ig）（IgM、IgG、IgA、IgE）、补体（complement,C）(C3、C4)、相关细胞因子[白细胞介素（interleukin,IL）-2、IL-6、IL-10、肿瘤坏死因子-α（tumor necrosis factor-α,TNF-α）]变化,并比较其与对照组的差异。结果 治疗前,病例组的CD16+CD56+、CD4、CD19、CD4/CD8、IgG、IgA、C3、C4显著低于对照组（P<0.05）,CD8、IL-2、IL-6、IL-10、TNF-α显著高于对照组（P<0.05）;治疗后,病例组的CD8、CD4/CD8、IgG、IgA、C3、C4、IL-2、IL-6、IL-10、TNF-α显著高于对照组（P<0.05）。治疗后病例组的CD16+CD56+、CD4、CD19、CD4/CD8、IgG、IgA、C3、C4、IL-2、IL-6、IL-10、TNF-α均显著高于治疗前（P<0.05）,CD8显著低于治疗前（P<0.05）,IgM、IgE与治疗前比较,差异无统计学意义（P>0.05）。结论 腺病毒肺炎可导致患儿免疫功能变化,免疫治疗有助于患儿免疫功能提升,控制患儿病情。     

Ⅲ期与Ⅳ期肺癌患者免疫功能比较及临床意义

目的测定肺癌患者外周血淋巴细胞及Th1、Th类细胞因子水平,探讨Ⅲ期与Ⅳ期肺癌患者免疫功能差异及临床意义。方法采用流式细胞仪测定293例首诊肺癌患者治疗前外周血CD3＋T、CD4＋T、CD8＋T、NK、B细胞以及INF-γ、IL-2、TNF-α、IL-4、IL-6、IL-10水平。结果Ⅲ期与Ⅳ期肺癌患者淋巴细胞与细胞因子水平均无差异,且各指标与病理类型、淋巴结转移无关（均P〉0.05）。结论晚期肺癌患者免疫力低下,机体免疫系统失去自身调节能力,肿瘤的免疫治疗至关重要。     

脾虚大鼠脑内IL-18蛋白及基因表达变化和益气扶正中药的干预研究

目的研究脾虚模型脑内白细胞介素18(IL-18)蛋白及基因表达变化和益气扶正中药的干预作用。方法 40只大鼠随机分成4组,正常组、模型组、治疗1组(四君子汤)、治疗2组(玉屏风散),每组各10只。采用苦降泻下、饮食失节加劳倦过度法建立脾虚大鼠模型,采用称重法检测免疫器官指数,采用免疫组化法与原位杂交法检测下丘脑腹侧核、海马CA1区的IL-18蛋白表达与IL-18mRNA表达变化及四君子汤、玉屏风散的治疗作用。结果模型组胸腺及脾脏指数明显下降,模型组IL-18蛋白与IL-18mRNA表达在下丘脑腹侧核、海马CA1区明显降低;治疗组胸腺及脾脏指数增加,上述脑区的IL-18蛋白与IL-18mRNA表达明显增加。结论益气扶正中药四君子汤、玉屏风散可能通过影响免疫器官,调控细胞因子IL-18蛋白及基因表达而调节机体免疫功能。     

脾虚证与益气健脾治疗对T、B淋巴细胞活性影响的实验观察

为探讨脾虚证机体特异性免疫应答的变化及益气健脾中药的影响,采用利血平注射法复制脾虚证模型,观察分别代表细胞与体液免疫功能的T、B淋巴细胞活性变化、血清IgG,IgM的水平及益气健脾基本方剂四君子汤对它们的影响,结果脾虚证模型大鼠T细胞转化率及B细胞分泌的IgM水平显著降低,通过灌服四君子汤可明显预防两者的降低,因而认为脾虚证细胞与体液免疫均降低,而以细胞免疫的降低为主,益气健脾中药具有明显防治作用。     

温针灸对亚健康肝气虚证职场女性T细胞亚群及细胞因子水平的影响

目的观察并评价温针灸对亚健康肝气虚证职场女性人群T细胞亚群及细胞因子水平的干预效果。方法选择亚健康肝气虚证职场女性60人,随机分为治疗组和对照组。两组均实施个体化健康教育,并辅助心理疏导。治疗组加用太冲、气海穴温针灸,连续干预4个疗程,用流式细胞仪法检测血中CD3、CD4、CD8水平,ELISA法检测IL-2、IL-6、TNF-α水平。结果经太冲、气海穴温针灸治疗4个疗程后,治疗组CD3、CD4、CD4/CD8比值与干预前相比明显提高(P<0.05),CD8、IL-2、IL-6、TNF-α水平与干预前比较明显下降(P<0.05),且均优于对照组(P<0.05)。结论对亚健康肝气虚证职场女性实施太冲、气海穴温针灸,可以调节T淋巴细胞亚群失衡,降低炎性细胞因子水平,改善免疫功能,提高机体免疫力。     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

Dr.Zhang Ren''''s Experience in the Acupuncture Treatment of Different Diseases with the Same Therapeutic Principle

Dr.Zhang Ren,the chief physician,is the chairman of Shanghai Acupuncture and Moxibustion Association.Having been engaged in medicine for about 40 years,he is experienced in treating various intractable diseases.In his long years of clinical practice,he advocates taking the TCM differentiation as the basis to seek for the acupuncture method for treatment of modern intractable diseases.The author of this essay had the fortune to follow Dr.Zhang in study.The following is a summary of Dr.Zhang's experience in the acupuncture treatment for different intractable diseases with the same therapeutic principle.     

Luo Lingjie''''s Experience in Treating Chronic Nephropathy

In treating chronic nephropathy,Luo Lingjie,a chief physician,pays attention to regulating the balance between yin and yang,treating infection if present,and removing pathogenic factors.He prescribes gentle drugs and uses carefully strongly warming-tonifying ones,emphasizes the importance of persuading the patient to persist in treatment with medication and nurse one's health for recuperation,and is good at combined use of TCM and western medicine therapy and brings the merits of various therapies into full play,with obvious theraoeutic effects.     

Acupuncture Combined with Spinal Tui Na for Treatment of Primary Dysmenorrhea in 30 Cases

Objective: To observe the therapeutic effects in acupunture treatment of primary dysmenorrhea combined with spinal Tui Na, and study its mechanism. Methods: Thirty cases of the treatment group were treated by acupuncture combined with spinal Tui Na, and thirty cases in the control group were treated by routine acupuncture. Results: The total effective rate was 93.3% in the treatment group, and 73.3% in the control group, with a significant difference between the two groups (P<0.05). Conclusions: Acupuncture combined with spinal Tui Na has good prospects for treatment of primary dysmenorrhea.     

猪肺磷脂注射液联合经鼻持续气道正压通气对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性的影响

目的 探讨猪肺磷脂注射液联合经鼻持续气道正压通气(NCPAP)对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性(CK-MB)的影响.方法 选取呼吸衰竭早产儿80例,分为观察组和对照组各40例.对照组采用NCPAP给氧治疗,观察组给予NCPAP给氧联合猪肺磷脂气管内给药.观察两组患儿治疗前及治疗12h、24 h后PaO2、PaCO2、血氧饱和度(SaO2)、pH的变化情况,检测治疗前及治疗5d后血清CK-MB水平;评估两组患儿的临床治疗效果.结果 两组患儿PaO2、PaCO2、SaO2、pH比较,差异均有统计学意义(P＜0.05),其中观察组治疗后的PaO2、SaO2、pH均高于对照组,PaCO2则低于对照组.两组的PaO2、SaO2、pH均随观察时间延长而升高(P＜0.05),PaCO2均随观察时间的延长而降低(P＜0.05).观察组治疗有效率为87.5％,显著高于对照组的70.0％ (P ＜0.05).治疗5d后两组患儿血清CK-MB水平均较前降低(P＜0.05),且观察组明显低于对照组(P＜0.05).结论 猪肺磷脂注射液气管内给药联合NCPAP可以显著降低呼吸衰竭早产儿CK-MB的含量,提高治疗有效率,起到很好的呼吸循环支持作用.     

Acupuncture Treatment of Vomiting

CASE HISTORY A female patient, 46 years old, head of the foreign affairs department of a certain university in Beijing, paid her first visit on October 9, 2006, with the chief complaint of vomiting for one month. She got vomiting after meals in early September. Before that, she had discomfortable sensation in the stomach due to angry with others, but she didn't pay much attention. Later, it developed into vomiting after eating. After the vomiting, the discomfort would be relieved, but with slight hypodynamia. She was once diagnosed as having 'neurogenic vomiting'. Having taken some western and Chinese drugs, the above symptoms were a little bit improved, but she would have nausea upon eating and with regurgitation. Because of the fear for vomiting, she did not dare to have food intake, with body weight reduction of 6 kilos in one month.     

Qiu Baoguo''''s Experience in Treating Consumptive Syndromes after Radio-Chemotherapies

Radiotherapy and chemotherapy are the important modern medical therapies for malignant tumors,yet they can also bring about serious local and systemic toxic side reactions so to decrease the patient;'s life quality,manifested by a series of consumptive symptoms.Having engaged in the combined work of Chinese and western medicine for nearly 50 years,the research fellow Qiu Baoguo in Henan Provincial Academy of TCM has developed his unique views on the TCM study of consumptive syndromes.The author of this essay had once the fortune tO follow Dr.Qiu in clinic,and specially would like to introduce in the following Dr.Qiu's experience in treating consumptive syndromes after radio-chemotherapies for patients with malignant tumor.     

Insomnia Due to Deficiency of Both the Heart and Spleen Treated by Acupuncture-Moxibustion and Chinese Tuina

OBJECTIVE: To observe therapeutic effects of the comprehensive therapy of acupuncture-moxibustion and Chinese Tuina for treatment of insomnia due to deficiency of both the heart and spleen. METHODS: 92 cases were divided randomly into the treatment group (treated by acupuncture-moxibustion and Chinese Tuina) and the control group (treated by acupuncture-moxibustion). RESULTS: The therapeutic effect of the treatment group was obviously superior to that of the control group (the CHI2 test showed P < 0.01). CONCLUSIONS: The comprehensive therapy of acupuncture-moxibustion and Chinese Tuina can give marked therapeutic effects for treatment of insomnia due to deficiency of both the heart and spleen.     

Survey and practice of reporting quality of randomized controlled clinical trials on traditional Chinese medicine

Evidence obtained from randomized controlled trials （RCTs） has been generally accepted as the gold standard in the evaluation of clinical effectiveness. Readers need to understand the trial design, implementation, results, analysis and interpretation, so as to fully Jnderstand the results of RCTs. Thus, the investigators of RCTs have to report these items in a complete, accurate and clear manner. Since 1998, we have conducted several evaluations on the reporting quality of RCTs published in Chinese journals on traditional Chinese medicine （TCM） and results have shown that there is an urgent need for higher quality RCTs on TCM.