单克隆抗体ELISA测定血浆富组氨酸糖蛋白的方法

建立用单克隆抗体（ＭｃＡｂ）酶联免疫吸附测定法（ＥＬＩＳＡ）测定血浆富组氨酸糖蛋白（ＨＲＧ）的方法。结果显示：ＭｃＡｂ３Ｃ６Ｆ３包被浓度为１０μｇ·ｍｌ－１，单抗酶标结合物（ＭｃＡｂ９Ｅ７Ｃ９ＨＲＰ）作１∶２００稀释时标准曲线最理想；最低检出限为１ｎｇ·ｍｌ－１，批内ＣＶ８２％，批间ＣＶ１１５％，回收率为８９１％，测定范围为２５～８０ｎｇ·ｍｌ－１；用该法测定４０例正常人血浆ＨＲＧ为（１１０３±９７）ｍｇ·Ｌ－１，２４例肝硬化患者血浆ＨＲＧ为（７９５±１２６）ｍｇ·Ｌ－１。该法的特异性强，且方便而准确。     

单克隆抗体ELISA测定血浆富组氨酸糖蛋白的方法

建立用单克隆抗体酶联免疫吸附测定法（ＥＬＩＳＡ）测定血浆富组氨酸糖蛋白（ＨＲＧ）的方法。结果显示：ＭｃＡｂ３Ｃ６Ｆ３包被浓度为１０μｇ·ｍｌ＾－１,单抗酶标结合物作１：２００稀释时标准曲线最理想;最低检出限为１ｎｇ·ｍｌ＾－１,批内ＣＶ８．２％,批间ＣＶ１１．５％,回收率为８９．１％,测定范围为２．５－８０ｎｇ·ｍｌ＾－１;用该法测定４０例正常人血浆ＨＲＧ为（１１０．３±９．７）ｍｇ·Ｌ＾－１,２     

高效液相色谱法同时测定盐酸维拉帕米及其主要代谢产物

建立了反相高效液相色谱法同时测定人血浆中维拉帕米及其主要代谢产物去甲维拉帕米血药浓度。以甲醇－水－三乙胺（６７∶３３∶０．４，ｐＨ６．７）为流动相，乙吗噻嗪（ｅｔｈｍｏｓｉｎｅ）为内标，样品用正己烷－正丁醇混合液提取浓缩后进样，紫外检测器检测（２７９ｎｍ）。此法操作简便，精密度好，日内、日间误差：维拉帕米＜８．６％，去甲维拉帕米＜７．６％；方法回收率高，维拉帕米、去甲维拉帕米回收率均＞９２％。两者血药浓度在２５～１０００ｎｇ·ｍｌ－１范围内呈线性关系，最小检测浓度维拉帕米：２．５ｎｇ·ｍｌ－１，去甲维拉帕米：５．０ｎｇ·ｍｌ－１。应用该法测定了６名志愿者口服盐酸维拉帕米片剂后的血药浓度     

高效液相色谱法测定人血浆中尼卡地平及药代动力学参数

建立了反相高效液相色谱法检测正常人口服尼卡地平片剂后的血药浓度。样品经硼酸缓冲液（ｐＨ９０）碱化后，用正己烷正丁醇混合溶剂（１２∶１，Ｖ／Ｖ）提取，以Ｃ１８化学键合硅胶为固定相，乙腈ＫＨ２ＰＯ４缓冲液（００１５ｍｏｌ／Ｌ，ｐＨ＝５５）三乙胺为流动相，尼莫地平为内标，在２３６ｎｍ波长定量检测。血药浓度在５～２００ｎｇ／ｍｌ范围内线性良好，最低检测浓度为２５ｎｇ／ｍｌ。日内（ｎ＝７）及日间（ｎ＝５）测定相对标准偏差分别小于５４％和６４％，血样测定回收率为９２８％１００８％。用本法监测了１０名健康人口服盐酸尼卡地平片剂后不同时间的血药浓度变化，测得血浆中尼卡地平浓度在１６±０３ｈ达到峰值１０９８±３８７ｎｇ／ｍｌ，Ｔ１／２＝５３５±２２８ｈ，血药浓度曲线下面积为３２２１±６９６ｎｇ·ｈ／ｍｌ。     

高效液相色谱法测定全血环孢霉素A

采用Ｃ１８反相色谱柱法测定定值全血（Ｂ１０－ＲＡＤ）,３个浓度的水平。结果均在预期范围内,其最低检出浓度为２５ｍｇ．ｍｌ＾－１,到嫠在５０～１００ｍｇ．ｍｌ＾－１范围内呈线性,绝对回收率为９９．６％～１０８．８％;批内和批间是系数分别为７．２％和９．３％。     

白细胞介素－1α诱导牛脑微血管平滑肌细胞增殖及药物的拮抗作用

作者研究了白细胞介素－１α（Ｉｎｔｅｒｌｅｕｋｉｎ－１α，ＩＬ－１α）对培养的牛脑微血管平滑肌细胞增殖的影响。在５～５００ｐｇ·ｍｌ－１范围内，ＩＬ－１α可诱导培养的牛脑微血管平滑肌细胞增殖，当用５０ｐｇ·ｍｌ－１ＩＬ－１α与该平滑肌细胞共孵育２４ｈ时，刺激作用最强，增殖率达２９．１％。欧芹素乙、异欧芹素乙，６－（α，α－苯基乙酰哌嗪基苯基）－４，５－二氢－５－甲基－３（２Ｈ）哒嗪酮在浓度为１０－６～１０－４ｍｏｌ·Ｌ－１时，可剂量依赖性地拮抗ＩＬ－１α诱导的该平滑肌细胞增殖，药物浓度最高（１０－４ｍｏｌ·Ｌ－１）时，抑制率值最大；６－（α，α－二苯基乙酰哌嗪基苯基）－４，５－二氢－５－甲基－３（２Ｈ）哒嗪酮，在低浓度时（１０－６和１０－５ｍｏｌ·Ｌ－１）可抑制ＩＬ－１α诱导的该平滑肌细胞增殖，药物浓度升高，抑制率也加大，而在高浓度时（１０－４ｍｏｌ·Ｌ－１）抑制率下降。     

反相高效液相色谱法测定饮料中的维生素E

用反相高效液相色谱法（ＨＰＬＣ）测定饮料中的维生素Ｅ（ＶＥ）含量。用正己烷提取,在Ｃ１８柱上进行分析,流动相选用甲醇,流速为１０ｍｌ·ｍｉｎ－１,用荧光检测仪在λｅｘｃ＝２９５ｎｍ,λｅｍ＝３３０ｎｍ处检测。ＶＥ浓度在０２～２２μｇ·ｍｌ－１范围内线性关系良好（ｒ＝０．９９９５,ｎ＝５）,回收率在９６８％以上。此法亦可用于其他食品中ＶＥ的分析。     

白细胞介素—1α诱导牛脑微血管平滑肌细胞增殖及药物的拮抗作用

作研究了白细胞介素－１α对培养的牛脑微血管平滑肌细胞增殖的影响。在５～５００ｐｇ～ｍｌ＾－１范围内，ＩＬ－１α可诱导培养的牛脑微血管平滑肌细胞增殖，当用５０ｐｇ·ｍｌ＾－１ＩＬ－１α与该平滑肌细胞共孵育２４ｈ时，刺激作用最强，增殖率达２９．１％。欧芹素乙、异欧芹素乙，６－（α，α－苯基乙酰哌嗪基苯基）－４，５－二氢－５－甲基－３（２Ｈ）哒嗪酮在浓度为１０＾－６～１０＾－４ｍｏｌ·Ｌ＾－１时，可剂     

高效液相色谱法测定奥美拉唑血浆药物浓度

用反相高效液相色谱法测定人体血浆中奥美拉唑的浓度。采用国产填料（ＹＷＧ－Ｃ１８，１０ｕｍ）反相色谱柱，１．０％三乙胺甲醇：水（６０：４０，Ｖ：Ｖ）为流动相，心律平为内标。紫外检测波长为３０２ｎｍ。方法回收率分别为９８．０３％（５０ｎｇ／ｍｌ）、１０１．５５％（４００ｎｇ／ｍｌ）和１０３．１３％（１０００ｎｇ／ｍｌ），相应变异系数（ＣＶ）分别为２．９６％（ｎ＝３）、１．９２％（ｎ＝３）和１．０４％（ｎ＝３）。本方法最小检测量为３ｎｇ，最低检测血药浓度为１０ｎｇ／ｍｌ。至少在５０ｎｇ／ｍｌ至１０００ｎｇ／ｍｌ范围内呈良好线性关系。本方法专一性强、灵敏度高、准确、简便、快速。     

神经酰胺诱导小鼠皮层神经元凋亡

本文采用改进的体外生物测定法分析恒河猴月经周期中促卵泡刺激素（ＦＳＨ）、促黄体生成素（ＬＨ）的变化情况。结果表明：雌猴的ＦＳＨ峰出现在月经周期的第１０２±１０天，峰值：５７２±４８ｎｇ／ｍｌ；ＬＨ峰出现在月经周期的第１２０±１６天，峰值：９６３±９７ｎｇ／ｍｌ，且为滤泡期、黄体期平均值的７～８倍。证实了在雌性恒河猴的月经周期中，其ＬＨ分泌高峰与ＦＳＨ分泌高峰不在同一时间出现的现象。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

Acupuncture Combined with Spinal Tui Na for Treatment of Primary Dysmenorrhea in 30 Cases

Objective: To observe the therapeutic effects in acupunture treatment of primary dysmenorrhea combined with spinal Tui Na, and study its mechanism. Methods: Thirty cases of the treatment group were treated by acupuncture combined with spinal Tui Na, and thirty cases in the control group were treated by routine acupuncture. Results: The total effective rate was 93.3% in the treatment group, and 73.3% in the control group, with a significant difference between the two groups (P<0.05). Conclusions: Acupuncture combined with spinal Tui Na has good prospects for treatment of primary dysmenorrhea.     

Luo Lingjie's Experience in Treating Chronic Nephropathy

In treating chronic nephropathy,Luo Lingjie,a chief physician,pays attention to regulating the balance between yin and yang,treating infection if present,and removing pathogenic factors.He prescribes gentle drugs and uses carefully strongly warming-tonifying ones,emphasizes the importance of persuading the patient to persist in treatment with medication and nurse one's health for recuperation,and is good at combined use of TCM and western medicine therapy and brings the merits of various therapies into full play,with obvious theraoeutic effects.     

Dr.Zhang Ren's Experience in the Acupuncture Treatment of Different Diseases with the Same Therapeutic Principle

Dr.Zhang Ren,the chief physician,is the chairman of Shanghai Acupuncture and Moxibustion Association.Having been engaged in medicine for about 40 years,he is experienced in treating various intractable diseases.In his long years of clinical practice,he advocates taking the TCM differentiation as the basis to seek for the acupuncture method for treatment of modern intractable diseases.The author of this essay had the fortune to follow Dr.Zhang in study.The following is a summary of Dr.Zhang's experience in the acupuncture treatment for different intractable diseases with the same therapeutic principle.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

猪肺磷脂注射液联合经鼻持续气道正压通气对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性的影响

目的 探讨猪肺磷脂注射液联合经鼻持续气道正压通气(NCPAP)对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性(CK-MB)的影响.方法 选取呼吸衰竭早产儿80例,分为观察组和对照组各40例.对照组采用NCPAP给氧治疗,观察组给予NCPAP给氧联合猪肺磷脂气管内给药.观察两组患儿治疗前及治疗12h、24 h后PaO2、PaCO2、血氧饱和度(SaO2)、pH的变化情况,检测治疗前及治疗5d后血清CK-MB水平;评估两组患儿的临床治疗效果.结果 两组患儿PaO2、PaCO2、SaO2、pH比较,差异均有统计学意义(P＜0.05),其中观察组治疗后的PaO2、SaO2、pH均高于对照组,PaCO2则低于对照组.两组的PaO2、SaO2、pH均随观察时间延长而升高(P＜0.05),PaCO2均随观察时间的延长而降低(P＜0.05).观察组治疗有效率为87.5％,显著高于对照组的70.0％ (P ＜0.05).治疗5d后两组患儿血清CK-MB水平均较前降低(P＜0.05),且观察组明显低于对照组(P＜0.05).结论 猪肺磷脂注射液气管内给药联合NCPAP可以显著降低呼吸衰竭早产儿CK-MB的含量,提高治疗有效率,起到很好的呼吸循环支持作用.