HPLC法测定异福胶囊的有关物质

目的 建立高效液相色谱法测定异福胶囊中有关物质的方法.方法 色谱柱为Kromasil C18,柱温为 40 ℃,流动相为甲醇-pH 7.0的磷酸盐缓冲液（0.01 mol·L-1磷酸二氢钾溶液,用0.1 mol·L-1氢氧化钠调pH）（60：40）,检测波长为254 nm,流速为1.0 mL·min-1,进样量为20 μL.结果 有关物质与主药色谱达到有效分离,1009011批样品中杂质I、醌式利福平、各杂质和分别为0、0.68%、3.2%.结论 本方法准确、灵敏度更高,可用于该制剂的有关物质检查.     

愈酚伪麻待因口服液对咳嗽变异性哮喘肺功能改善效果的观察

目的探讨愈酚伪麻待因口服液对咳嗽变异性哮喘（CVA）肺功能改善的效果。方法对36例咳嗽变异性哮喘患者使用愈酚伪麻待因口服液治疗前后的肺功能改善数据进行对比分析（FVC、FEV10。PEF、V75、V50、V25）。结果CVA患者存在不同程度的肺功能受损,治疗前后肺功能指标有显著性差异。结论CVA患者使用愈酚伪麻待因口服液治疗后肺功能得到改善。临床症状缓解。     

愈酚伪麻口服溶液化痰止咳作用的观察

目的 探讨愈酚伪麻口服溶液的临床疗效及安全性.方法 120例12～12周岁急性上呼吸道感染,急性支气管炎,肺炎伴咳嗽、咳痰、鼻塞、流涕等症状的患儿随机分为治疗组(愈酚伪麻口服溶液)和对照组(肺力咳合剂),两组的其它治疗相同.观察两组治疗前及治疗后3d、5d咳嗽、痰液粘稠、排痰困难评分及综合评分变化.结果 两组治疗前后效果比较疗效显著,但治疗组咳嗽、咳痰、鼻塞、流涕各项指标疗效及综合疗效评价与对照组比较差异显著,治疗组治疗5d后呼吸道症状改善的总有效率高于对照组.结论 愈酚伪麻口服溶液是一种安全有效的祛痰止咳药.     

愈酚甲麻那敏分散片的人体药动学研究

目的建立测定人血浆中愈创木酚甘油醚的高效液相法,以研究健康志愿者口服愈酚甲麻那敏分散片后愈创木酚甘油醚的药动学过程。方法色谱条件：Agilent Eclipse XDB—C18为色谱柱,以乙腈-磷酸二氢铵（体积比20：80,浓磷酸调至pH值3．0）为流动相,氧去甲右美沙芬为内标,紫外检测波长为230nm。结果愈创木酚甘油醚质量浓度在25～2000ng·mL^-1范围内线性关系良好（r=0．9977）,定量下限为25ng·mL^-1。20名受试者单剂量口服愈酚甲麻那敏分散片后的主要动力学参数Cmax为（474±142）ng·mL^-1,tmax为（0．275±0．123）h,t1/2为（0．83±0．55）h,AUC0→4为（451±165）ng·h·mL^-1,AUC0→∞为（505±159）ng·h·mL^-1。结论本文所建立的方法简便、准确、可靠,可满足愈创木酚甘油醚临床药动学研究的需要。     

愈酚伪麻口服溶液化痰止咳作用的观察

目的探讨愈酚伪麻口服溶液的临床疗效及安全性。方法 120例2～12周岁急性上呼吸道感染,急性支气管炎,肺炎伴咳嗽、咳痰、鼻塞、流涕等症状的患儿随机分为治疗组（愈酚伪麻口服溶液）和对照组（肺力咳合剂）,两组的其它治疗相同。观察两组治疗前及治疗后3d、5d咳嗽、痰液粘稠、排痰困难评分及综合评分变化。结果两组治疗前后效果比较疗效显著,但治疗组咳嗽、咳痰、鼻塞、流涕各项指标疗效及综合疗效评价与对照组比较差异显著,治疗组治疗5d后呼吸道症状改善的总有效率高于对照组。结论愈酚伪麻口服溶液是一种安全有效的祛痰止咳药。     

HPLC法测定家兔模型肠梗阻后血浆中瓜氨酸含量

目的:利用柱前衍生法测定家兔肠梗阻后血浆中瓜氨酸含量,建立简便、快速的测定方法.方法:以异硫氰酸苯酯(PITC)作为柱前衍生剂,采用高效液相色谱法,流动相为0.072 mol· L-1的乙酸钠水溶液:甲醇:乙腈=900:75:25(再加入10 mmol· L-1的EDTA溶液125 μL),流速为0.8 mL· min-1,检测波长为254 nm,柱温为室温.结果:最低检测限0.57 μmol· L-1,瓜氨酸在5.71～1 141.62 μmol·-1范围内呈较好线性,回归方程为Y=2 490.6x-26 068(r=0.999 1,n=3),平均加样回收率为100.02％.结论:该方法能较好地测定出瓜氨酸的含量,且重复性好、稳定性高、方法简单实用,适用于血浆中瓜氨酸浓度的检测.     

RP-HPLC法测定阿立哌唑片剂中的阿立哌唑及有关物质

目的 :建立测定阿立哌唑片剂中阿立哌唑及有关物质的检测方法。方法 :采用反相高效液相色谱法 ,色谱柱为十八烷基硅烷键合硅胶柱 ,流动相为 0 .0 5mol·L-1 磷酸二氢钠水溶液 (含 1%三乙胺 ,磷酸调 pH至 5 .5 ) 乙腈 (5 0∶5 0 ) ,检测波长为2 17nm ,流速为 1.0ml·min-1 。结果 :阿立哌唑在 4.10 6～ 41.0 60mg·L-1 浓度范围内峰面积有良好线性关系 ,平均回收率为99 .90 %(n =9) ,重复性试验的相对标准偏差为 0 .82 %(n =6) ,最低检出限量为 0 .5 1ng ,有关物质与主药有较好的分离度。结论 :此法简单、专属性强、重现性好、结果准确可靠 ,适用于阿立哌唑片的质量控制。     

盐酸尼非卡兰的有关物质及含量测定方法

目的 分别用HPLC和非水酸碱滴定法建立起盐酸尼非卡兰有关物质检查及含量测定的方法,为原料的质量控制提供有效的分析方法.方法 采用岛津CLC-ODS(6 mm×150 mm,5 μm)柱,流动相:乙腈-0.02 mol·L-1的磷酸二氢钾溶液(NaOH溶液调pH至5.0)=25∶75;检测波长:268 nm;流速:1.0 mL·min-1;柱温:30℃.结果 在选定的色谱条件下,盐酸尼非卡兰与7个有关物质分离完全;盐酸尼非卡兰在5～100 mg·L-1内,峰面积与浓度线性关系良好,r=0.999 9;检测限为0.2 ng.结论 方法简便,准确,专属性强,可用于盐酸尼非卡兰的有关物质检查及含量测定.     

高效液相色谱测定尿中香草扁桃酸含量

目的 :建立测定尿中的香草扁桃酸 (VMA)含量的高效液相色谱法。方法 :以Nova PakC18柱为分析柱 ,流速是 0 9ml·min-1,流动相为 40mmol·L-1NaH2 PO4 液 (内含Na2 EDTA和辛基磺酸钠各为 5 0mg·L-1,pH3.0 ) ,采用电化学检测器和利用内标法测定了 30例正常人 2 4h尿中的VMA含量。结果 :当VMA浓度为 5～ 2 0 0 μmol·L-1时呈良好的线性关系。VMA的平均回收率为 99 1% ,平均批内变异系数为 5 8% ,平均批间变异系数为 7 7% ,最低检出量是 5 3 2μg。标本量为 10 0 μl时的最低检出浓度为 0 2 8μmol·L-1;正常人 2 4h尿中VMA含量为 (18 13± 6 17) μmol。结论 :该方法能准确测定尿中VMA含量 ,适合临床常规应用。     

HPLC法测定注射用甲硫氨酸维B_1有关物质

目的:测定注射用甲硫氨酸维B_1中有关物质的方法。方法:采用高效液相色谱法,色谱柱C_(18)ODS-1,Kromasil,4.6mm×250mm,流动相为甲醇-乙腈-0.020mol/L庚烷磺酸钠溶液(含1%三乙胺溶液)(用磷酸调pH值至5.5)(9:9:82)。检测波长:220nm,流速1mL/min。结果:甲硫氨酸与维B_1及其二者降解产物能有效分离,甲硫氨酸最低检出量为22ng,维生素B_1最低检出量为10ng,注射用甲硫氨酸维B_1成品控制总杂质不超过1.5%。结论:本法准确、简便、专属,适用于注射用甲硫氨酸维B_1的有关物质检查。     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

Dr.Zhang Ren''''s Experience in the Acupuncture Treatment of Different Diseases with the Same Therapeutic Principle

Dr.Zhang Ren,the chief physician,is the chairman of Shanghai Acupuncture and Moxibustion Association.Having been engaged in medicine for about 40 years,he is experienced in treating various intractable diseases.In his long years of clinical practice,he advocates taking the TCM differentiation as the basis to seek for the acupuncture method for treatment of modern intractable diseases.The author of this essay had the fortune to follow Dr.Zhang in study.The following is a summary of Dr.Zhang's experience in the acupuncture treatment for different intractable diseases with the same therapeutic principle.     

Luo Lingjie''''s Experience in Treating Chronic Nephropathy

In treating chronic nephropathy,Luo Lingjie,a chief physician,pays attention to regulating the balance between yin and yang,treating infection if present,and removing pathogenic factors.He prescribes gentle drugs and uses carefully strongly warming-tonifying ones,emphasizes the importance of persuading the patient to persist in treatment with medication and nurse one's health for recuperation,and is good at combined use of TCM and western medicine therapy and brings the merits of various therapies into full play,with obvious theraoeutic effects.     

Acupuncture Combined with Spinal Tui Na for Treatment of Primary Dysmenorrhea in 30 Cases

Objective: To observe the therapeutic effects in acupunture treatment of primary dysmenorrhea combined with spinal Tui Na, and study its mechanism. Methods: Thirty cases of the treatment group were treated by acupuncture combined with spinal Tui Na, and thirty cases in the control group were treated by routine acupuncture. Results: The total effective rate was 93.3% in the treatment group, and 73.3% in the control group, with a significant difference between the two groups (P<0.05). Conclusions: Acupuncture combined with spinal Tui Na has good prospects for treatment of primary dysmenorrhea.     

猪肺磷脂注射液联合经鼻持续气道正压通气对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性的影响

目的 探讨猪肺磷脂注射液联合经鼻持续气道正压通气(NCPAP)对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性(CK-MB)的影响.方法 选取呼吸衰竭早产儿80例,分为观察组和对照组各40例.对照组采用NCPAP给氧治疗,观察组给予NCPAP给氧联合猪肺磷脂气管内给药.观察两组患儿治疗前及治疗12h、24 h后PaO2、PaCO2、血氧饱和度(SaO2)、pH的变化情况,检测治疗前及治疗5d后血清CK-MB水平;评估两组患儿的临床治疗效果.结果 两组患儿PaO2、PaCO2、SaO2、pH比较,差异均有统计学意义(P＜0.05),其中观察组治疗后的PaO2、SaO2、pH均高于对照组,PaCO2则低于对照组.两组的PaO2、SaO2、pH均随观察时间延长而升高(P＜0.05),PaCO2均随观察时间的延长而降低(P＜0.05).观察组治疗有效率为87.5％,显著高于对照组的70.0％ (P ＜0.05).治疗5d后两组患儿血清CK-MB水平均较前降低(P＜0.05),且观察组明显低于对照组(P＜0.05).结论 猪肺磷脂注射液气管内给药联合NCPAP可以显著降低呼吸衰竭早产儿CK-MB的含量,提高治疗有效率,起到很好的呼吸循环支持作用.     

Survey and practice of reporting quality of randomized controlled clinical trials on traditional Chinese medicine

Evidence obtained from randomized controlled trials （RCTs） has been generally accepted as the gold standard in the evaluation of clinical effectiveness. Readers need to understand the trial design, implementation, results, analysis and interpretation, so as to fully Jnderstand the results of RCTs. Thus, the investigators of RCTs have to report these items in a complete, accurate and clear manner. Since 1998, we have conducted several evaluations on the reporting quality of RCTs published in Chinese journals on traditional Chinese medicine （TCM） and results have shown that there is an urgent need for higher quality RCTs on TCM.     

TCM Treatment of Ankylosing Spondylitis by Tonifying the Kidney and Strengthening the Governor Vessel

Ankylosing spondylitis is a chronic and progressive disorder with inflammation mainly involving the central axis joints. It mainly affects the cervical spine and the lumbosacral area, with the pathogenesis closely related to the kidney and the Governor Vessel (GV). TCM holds that the syndrome is deficiency in origin and excess in superficiality, which is due to insufficiency of the kidney, deficiency of GV, and blocking of the channels with the invasion of exogenous evil, leading to poor circulation of qi and blood and malnutrition of the bones, muscles and joints. The TCM method of tonifying the kidney and strengthening GV to regulate circulation of qi and blood and check the arthralgia pain should be adopted, with the Kidney-Tonifying and GV Strengthening Decoction (益肾强督汤) prescribed.     

IMPROVING P-gp EXPRESSION IN HUMAN MONONUCLEAR CELLS IN VITRO TRANSFECTED BY MULTIDRUG RESISTANCE-1 mRNA

CHEMOTHERAPY playsa greatrolein the treat- ment of malignanttumors,especiallyingynecolo- gicalones.But inanticancerchemotherapy,leuko-cytopeniaisfrequentlytheprimarydose-limitingsideeffect factor.Moreover,cancersarefrequentlychemoresistantbe-causeof overexpressionof P-glycoprotein(P-gp), which isencodedby multidrugresistancegene (MDR1 ) and detectableinup to50% ofhuman cancersand renderscellsresistancetoanticancerdrugs.The safetyand potentialtherapeuticbenefitof mdr1 gene transferredto h…