顺铂腹腔化疗对卵巢癌模型鼠雌激素影响的实验研究

目的观察鼠卵巢癌模型在顺铂化疗前后雌激素水平的变化,评估顺铂腹腔化疗对卵巢功能的影响。方法取8周龄裸鼠36只,随机分为空白对照组(A组,n=8)、肿瘤细胞接种后不化疗组(B组,n=12)、肿瘤细胞接种后静脉化疗组(C组,n=8)、肿瘤细胞接种后腹腔化疗组(D组,n=8)。所有裸鼠均行一侧附件切除术,除A组外,术中向其余3组鼠腹腔内注射卵巢癌细胞悬液。于术后第9天对A组和B组裸鼠腹腔内注入生理盐水,C组裸鼠尾静脉注射顺铂(20μg/g)+腹腔内注射生理盐水,D组裸鼠腹腔内注射顺铂(20μg/g)。第1次化疗后第0、10、20天分别取鼠尾静脉血测量雌激素水平。化疗22d后重复第1次化疗方法1次。于第2次化疗后第10、20及30天分别取鼠尾静脉血测量雌激素水平。结果腹腔内接种肿瘤细胞后B、C及D组裸鼠雌激素水平明显高于A组(P＜0.05),A与B组的雌激素水平相对稳定,C和D组雌激素水平在化疗后显著降低,但C组高于D组(P＜0.05),随着化疗后时间延长,C与D组裸鼠雌激素水平逐渐下降至A组水平(P＞0.05)。结论 顺铂化疗会损伤卵巢功能,腹腔化疗对卵巢皮质的损伤可能大于静脉化疗,随着化疗间隙期的延长卵巢功能可以得到恢复。     

环磷酰胺化疗导致小鼠卵巢功能损伤模型的建立

目的探索建立化疗后导致小鼠卵巢功能损伤模型的方法。方法以C57BL/6雌性小鼠为研究对象,以75 mg/kg的剂量一次性腹腔注射环磷酰胺,观察小鼠一般情况、动情周期、血清雌激素(雌二醇)水平以及卵巢组织的形态学改变、各级卵泡数变化、抗苗勒管激素分泌情况和组织纤维化程度。以未注射环磷酰胺小鼠作为对照。结果与对照组比较,环磷酰胺注射小鼠血清雌二醇水平降低,卵巢组织抗苗勒管激素分泌减少,卵巢组织间质增生、纤维化明显,各级卵泡数目减少。结论 75 mg/kg的剂量一次性腹腔注射环磷酰胺后可引起小鼠明显的卵巢功能损伤,小鼠生殖内分泌和卵巢组织学的变化与临床上化疗导致卵巢功能损伤患者的情况相似。该方法简便易行、造模时间短、成功率高、死亡率低,是建立化疗导致卵巢功能损伤动物模型的较好方法。     

非清髓性预处理联合髓腔内骨髓细胞输注诱导免疫耐受的实验研究

目的本研究通过非清髓性预处理方案联合髓腔内骨髓移植建立异基因小鼠免疫耐受模型,并探讨其诱导耐受的机理.方法受鼠C57BL/6(B6)于第0天接受60Coγ射线全身照射(TBI,550-cGy),4 h内输注雄性BALB/C(H2d)小鼠来源的骨髓细胞,2 d后腹腔注射环磷酰胺(CTX,200 mg·kg-1).通过皮肤移植、混合淋巴细胞反应(mixed lymphocyte reaction, MLR)检测耐受状态,并应用体外过继转移实验、IL 2逆转实验等探讨免疫耐受机制.结果经骨髓移植的B6小鼠对BALB/C小鼠的皮肤移植物平均存活时间超过300d,较空白组明显延长(P＜0.001);MLR结果证明B6小鼠获得供体特异性耐受,该耐受可以被IL 2逆转且可被过继转移;所有受鼠均未出现GVHD表现.结论非清髓预处理联合髓腔内骨髓移植可以有效诱导异基因小鼠的免疫耐受.     

大小鼠之间造血干细胞嵌合体抗异种移植物抗宿主病

目的探讨克服异种骨髓移植间强烈的移植物抗宿主病(GVHD)的措施。方法(1)将12只SD大鼠经5.0 Gy亚致死剂量全身照射后,4 h内每只经尾静脉输入28只正常BALB/c小鼠骨髓细胞8×107个。2 d后从腹腔注射环磷酰胺(Cy)100 mg/kg·b.w.,诱导形成对BALB/c小鼠产生特异性免疫耐受的嵌合体大鼠10只。(2)24只BALB/c小鼠接受9.0 Gy致死剂量全身照射后随机均分为3组,照射后4 h内经尾静脉注射骨髓细胞进行移植。A组输注正常SD大鼠的骨髓细胞4×107个;B组输注正常SD大鼠的骨髓细胞4×107个和脾细胞2×107个;C组输注嵌合体SD大鼠的骨髓细胞4×107个和脾细胞2×107个;观察GVHD的发生情况。结果A组有2只小鼠死于感染和放射损伤,所有对象均未观察到明显GVHD表现。B组平均累积存活时间为10 d[95%可信区间为(8,12)],死前均出现不同程度的典型GVHD表现。而C组除2只小鼠分别于移植后18、31 d死亡外,其余的均存活超过150 d。三组间比较有统计差异(P<0.002)。结论对有受者嵌合体的供者进行异种骨髓移植,有助于克服异种移植物抗宿主病。     

非清髓性骨髓移植的动物实验研究

目的　建立非清髓性骨髓移植的小鼠动物模型 ,探讨非清髓预处理条件下能否实现异基因造血细胞的植活 ,探讨输入的异基因造血细胞数量和移植植活的关系。方法　以雌性C5 7BL/ 6小鼠为异基因骨髓移植的受者 ,雄性BALB/c小鼠为异基因骨髓移植的供者 ,采用非致死剂量的环磷酰胺腹腔注射 (2 0 0mg/kg)加γ射线全身照射(5Gy)为非清髓性预处理方案 ,将移植受鼠分为A、B、C 3组 ,每组 10只 ,分别给予 2× 10 7、8× 10 6和 2× 10 6个供鼠骨髓细胞 ,移植后第 36天用聚合酶链式反应检测受鼠骨髓细胞内Y染色体特异性基因检测移植物植活。结果　在移植A、B和C组中分别有 8、3和 0只小鼠检测到了异基因造血细胞的植活 ,经过统计学检验发现A组小鼠的植活比例高于其余两组。结论　非清髓的预处理条件下可以实现异基因骨髓移植的成功植入 ;加大移植物中造血前体细胞的数量有助于克服移植供、受者间主要组织相容性 (MHC)间的差异和促进移植物植活     

生血增白汤对环磷酰胺致血虚小鼠RBC、Hb、WBC、Plt含量的影响

目的研究生血增白汤对环磷酰胺(CTX)致血虚小鼠RBC、Hb、WBC、Plt含量的影响,旨在探讨生血增白汤对化学性损伤血虚症的补血作用。方法取ICR小鼠60只,随机分为6组,即正常对照组(A组),模型对照组(B组),阳性药四物合剂组(C组),生血增白汤低、中、高剂量组(D1、D2、D3组),每组10只,连续给药8 d。除A组外,其他组小鼠每日均于给药后4 h注射环磷酰胺100 mg/kg,第8天给药后测全血红细胞(RBC)、血红蛋白(Hb)、白细胞(WBC)、血小板(Plt)值,并对数据进行统计学分析。结果 B组小鼠RBC、Hb、WBC、Plt含量较A组显著降低(P0.01)。与B组比较,D3组能使血虚证小鼠Hb升高(P0.05);D1、D2、D3组及C组能使血虚证小鼠WBC升高(P0.01);D3组及C组能升高小鼠Plt(P0.05)。结论生血增白汤对CTX致化学性损伤造成的血虚证模型小鼠具有补血作用。     

环磷酰胺诱导家兔卵巢早衰动物模型的建立和评价

目的比较不同注药方式下建立环磷酰胺诱导家兔卵巢早衰动物模型的方法,寻找最佳造模方式,为进一步研究卵巢早衰提供更为适用的实验模型。方法 21只家兔随机分四组,正常对照组3只,不作任何处理(A组);模型一组6只,50 mg/kg环磷酰胺溶液注射1 d(B组);模型二组6只,50 mg/kg连续注射2 d(C组);模型三组6只,首日注射50 mg/kg,以后按照8 mg/kg连续注射14 d(D组)。称量各组家兔体重和卵巢重量,描述注药14 d内的体重变化,分析卵巢指数;HE染色观察卵泡形态,光镜下计算各级卵泡数;TUNEL法观察分析细胞凋亡情况;ELISA法分析血清雌二醇(E2)变化。结果体重:B、C组体重无明显变化(P0.05);D组家兔生长受限(P0.05),死亡率高。卵巢指数:C组卵巢指数明显小于A组(P0.05)。各级卵泡情况:B、C组原始卵泡和初级卵泡异常率均升高(P0.017),其中C组原始卵泡异常率升高更明显(P0.017),而两组初级卵泡异常率差异无显著性(P0.017)。A、B、C三组次级、窦状卵泡异常率差异无显著性(P0.05)。细胞凋亡率:细胞凋亡主要发生在卵泡中的颗粒细胞上,B、C组凋亡率均明显高于A组(P0.05),且C组凋亡率高于B组(P0.05)。血清E2值:B组第7天E2分泌达高峰,7 d后逐渐下降,E2分泌量第7天明显高于第35天(P0.05);C组E2值持续下降,第0天明显高于第35天(P0.05)。结论以50 mg/kg剂量连续注射2 d是建立环磷酰胺诱导家兔卵巢早衰模型的最佳造模方式。     

七叶灵对 Lewis 肺癌细胞荷瘤小鼠作用的初步观察

为观察七叶灵(由生黄芪、女贞子、天花粉、黄精、石见穿、七叶一枝花、灵芝组成)对 Lewis 肺癌细胞(3LL)荷瘤小鼠的作用,将40只 C57小鼠以 3LL 细胞皮下接种(80×10/只),1周后随机均分为对照组(A)、化疗组(B)、化疗加中药组(C)和中药组(D)4组,并予相应的处理,A 组腹腔注射生理盐水,B、C 组腹腔注射环磷酰胺(CTX,0.2mg*d-1*只-1),连续2d,C、D 组以七叶灵煎剂灌胃(0.4ml*d-1*只-1),连续2个月.每周统计死亡率,测量肿瘤体积并换算成重量.结果第2,3周时,B、C、D 组肿瘤的重量明显低于 A 组,第4,5,6周仅 C组肿瘤重量低于 A组,但至第7周.各组于第48天开始出现死亡,A组于第28天全部死亡,B 组,至第63天全部死亡,C 组于第77天全部死亡,D 组于第132天才全部死亡,组间比较差异明显.提示,七叶灵能延长肿瘤鼠的生存时间,其与化疗药物合用能抑制肿瘤的生长.     

顺铂腹腔注射对小鼠卵巢相关基因表达谱的影响

目的：应用基因芯片技术研究顺铂（Clsplatin）腹腔注射给药对小鼠卵巢相关基因表达的影响,探讨顺铂对卵巢功能损伤的作用机制。方法：建立化疗损伤后卵巢功能低下小鼠模型,按一步法抽提顺铂给药后的小鼠及对照组小鼠卵巢组织的总RNA;经逆转录分别用Cy3、Cy5荧光标记,获得两组小鼠卵巢cDNA的探针,再与cDNA基因表达谱芯片杂交,结果由激光扫描仪扫描,并用计算机软件进行图像分析、标准化处理、ratio值分析和聚类分析。结果：顺铂给药后的小鼠与对照组小鼠卵巢组织基因表达谱分析发现,109个差异表达基因有19个基因低表达,90个基因高表达。结论：本研究从顺铂给药后的小鼠卵巢组织中筛选出大量的差异表达基因,说明这些基因可能参与顺铂对卵巢功能损伤的发生、发展过程,从而为化疗药物损伤性卵巢功能减退的诊治提供依据。     

皮下注射骨髓细胞诱导小鼠皮肤移植后免疫耐受

目的　探讨皮下注射供体骨髓细胞加用环孢素A(CyA)短期免疫抑制的方法 ,诱导小鼠皮肤移植后免疫耐受的可行性。方法　通过尾 尾皮肤移植将 8周龄BABL/c小鼠皮肤移植到同龄C57BL/ 6小鼠上 ,后者分为 5组 ,A组长期应用CyA ,B～E组按不同时间和剂量皮下注射供体骨髓细胞并加用短期CyA腹腔注射 (7～ 1 1d) ,观察C57BL/ 6小鼠移植皮肤综合评分及平均生存时间。结果　B、C两组小鼠 (BM 1× 1 0 5,1× 1 0 6 ,0～ 1 1d隔天注射 ) ,停用CyA后短期出现排斥反应 ,移植皮肤平均存活 2 7d ;D、E两组小鼠 (BM 1× 1 0 7,0、4、8、1 1d注射和BM 1× 1 0 8,0、5、1 0d注射 ) ,停用免疫抑制剂后并未发生早期排斥反应 ,移植皮肤平均存活时间明显延长(48、55d) ,与持续使用CyA的A组 (54d)相近。 结论　在该小鼠皮肤移植模型中 ,供体骨髓细胞皮下注射加短期免疫抑制剂CyA诱导出了受体对同种皮肤移植物暂时性的耐受 ,延长了移植物平均存活时间 ;该耐受的形成与骨髓细胞注射剂量、间隔时间相关 ,大剂量间隙注射更可能诱导免疫耐受。     

肝细胞生长因子对骨髓移植小鼠造血重建的促进作用

目的 观察肝细胞生长因子（HGF）对骨髓移植小鼠造血重建的促进作用。方法 用BALB／c小鼠建立同基因骨髓移植模型，随机将BALB／c小鼠分A、B两组，B组移植后叶7d皮下注射HGF，A组皮下注射PBS，移植后1、7、14、21和28d检测小鼠血红蛋白、白细胞、血小板、骨髓单个核细胞及骨髓造血细胞面积。结果 B组血红蛋白在移植后7、14d与同期A组无差异（P〉0．05），B组血红蛋白在移植后21d高于同期A组（P〈0．05）；B组血小板在移植后7、14及21d高于同期A组（P值分别小于0．01、0．01和0．05）：B组白细胞在移植后7、14、21及28d高于同期A组（P值分别小于0．01、0．01、0．05及0．05）；B组骨髓单个核细胞及骨髓造血细胞面积在移植后7、14、21和28d高于同期A组（P值分别小于0．05、0．01、0．01及0．05）。结论 HGF对骨髓移植小鼠造血重建有一定的促进作用。     

FTY720对小鼠异基因骨髓移植后急性GVHD的预防作用

目的 评估FTY720对小鼠异基因骨髓移植诱发急性移植物抗宿主病（aGVHD）的预防作用。方法36只受体BALB／c小鼠,接受直线加速器一次性全身照射后4～6h内,经尾静脉注射供体小鼠骨髓和脾脏细胞。受体小鼠分为移植方式对照组（A组）和异基因移植治疗组（B组）;同时A组分未移植组（A1）、同基因移植组（A2）和异基因移植对照组（A3）,B组分CsA5mg／kg组（B1）,FTY7200．3mg／kg组（B2）和FTY7201mg／kg组（B3）。移植后受体小鼠,根据生存时间、体重变化和病理组织学检查,评估各组aGVHD严重程度。结果 异基因移植各组在移植后第7d造血开始恢复,第14d流式细胞仪检测显示异基因骨髓细胞已植入,病理组织学检查A3组发生重度aGVHD;与B1和B2组比较,B3组aGVHD严重程度最低。结论小鼠异基因骨髓移植后给予FTY720具有预防aGVHD作用．以剂量为1mg／kg时作用显著。     

Effect of ligustrazine on hematopoiesis in bone marrow transplantation mice

Hematopoietic reconstitution after bone marrowtransplantation is a complicated process,whichconcerns with the quality and quantity ofhematopoietic stem cell (HSC) ,and also with therecovery of hematopoietic inducing microenvironment(HIM) .The contact of HSC and HIM is critical forhematopoietic reconstitution.1　MATERIALSAND METHODS1 .1　Materials1 .1 .1　 Animals　　 BALB/c mice of both sexes(clean class,close copulatory series (H- 2 d,MLSb) )were 8— 1 0 weeks old,and 1 7— 2 …     

Study on blocking the leukemia immune escape after BMT by Fas-Fas ligand pathway

Background To investigate if bone marrow transplantation (BMT) with bone marrow mononuclear cells (BMMCs) transducted with murine soluble Fas gene (sFas) using adenovirus vector could block the immune escape of leukemia cells eliminate the residual leukemia cells and reduce their relapse.Methods The recombinant adenovirus vector with murine sFas, adsFas, and the control vector adEGFP were constructed using homologous recombination between two plasmids in Escherichia coli. BMT was carried out after the BMMCs were infected with Adenoviruses. The mice models of leukemia/lymphoma were constructed by inoculating female C57BL/6 mice (H-2b) with 10（5） EL4 cells/mouse through caudal vein. Donors of bone marrow grafts were syngeneic male mice. BMMCs were infected with AdsFas or AdEGFP 24 hours before (Group D or E). The following three groups were simultaneously used: Group A, no BMMCs transplanted; Group B, transplanted with BMMCs not infected with adenoviruses; Group C, only transfusing EL4 cells, neither irradiation nor BMT. The hematopoietic reconstitution, generation of leukemia/lymphoma and the survival rate were observed in all groups after BMT. Results The adenovirus vectors were successfully constructed. The titre of virus after purification was up to 2.5×10（11）pfu/ml. Spleen indices examined 11 days after BMT were not obviously different among Group B, D and E (P&gt;0.05), but indices in Group A were significantly lower than those in the latter three groups (P&lt;0.01). Counts of leukocytes and platelets on +30 day showed mice were reconstituted satisfactorily in Group B and D, but very low in Group C and E. The Y-chromosomes existed 2 months after BMT and examination of bone marrow cytology showed that Group B and D were almost normal, but Group C and E had plenty of lymphoblast-like tumor cells. Tumors were obviously observed in the mice of Group C and E by histopathological examination, but the mice in Group B and D were normal. The survival rates were 0 (0/4) in Group A, 100% in Group B (6/6) and D (16/16), 12.5% (2/16) in Group C and 6.25% (1/16) in Group E respectively. It is demonstrated that, in contrast with the control (Group EGFP), survival rate was significantly increased in the sFas Group (P&lt;0.01). Conclusions The transfer of sFas gene by adenovirus changed the prognosis state of leukemia/lymphoma mice after auto-BMT. The transduction of sFas might block the effect of the immune escape of EL4 cells through FasL. These results could thus provide a new direction to find a way to treat the leukemia and its recurrence after BMT.     

大小鼠之间造血干细胞嵌合体抗异种移植物抗宿主病

OBJECTIVE: To find a method for preventing graft-versus-host disease (GVHD) in BALB/c mice receiving rat bone marrow transplantation. METHODS: Firstly 12 SD rats were conditioned with 5.0 Gy sublethal total body irradiation(TBI), followed by infusion of 8 x 10(7) bone marrow cells from 28 BALB/c mice within 4 h and intraperitoneal administration of 100 mg/kg cyclophosphamide (Cy) 2 d later, for the purpose of inducing chimeric SD rats with specific immunological tolerance. Secondly, 24 BALB/c mice were exposed to 9.0 Gy lethal TBI and divided randomly into 3 equal groups designated respectively as groups A, B and C. Mice in group A were injected with 4 x 10(7) bone marrow cells from 4 normal rats, and mice in group B were injected with 4 x 10(7) bone marrow cells and 2 x 10(7) spleen cells from 4 normal rats, while those in group C were given 4 x 10(7) bone marrow cells and 2 x 10(7) spleen cells from 6 chimeric rats. The mice were then observed for 150 d for GVHD. RESULTS: In the second procedure, 2 mice in group A failed to survive due to infection and radiation injury respectively, and none of the rest mice presented signs of GVHD. The mice in group B all developed GVHD of varied degrees with symptoms as wasting, diarrhea, fur loss, arched body posture, and even bloody stool, and all died within 5 to 15 d with an average survival of 10.0 d (95% confidence interval 8,12). Pathological examination of the liver and intestinal tissues revealed inflammatory lymphocyte infiltration and necrosis. The majority of the mice in group C, in contrast, survived for more than 150 d with only two died on the postoperative days 18 and 31 respectively. The survival time of group B was significantly shorter than that of the other two groups. CONCLUSION: Donor/recipient chimerism may help prevent GVHD in xenogeneic bone marrow transplantation.     

半相合细胞移植对CT-26结肠癌荷瘤小鼠的抑瘤作用

目的:探讨半相合细胞移植的移植物对CT-26结肠癌荷瘤小鼠的抑瘤作用及潜在机制.方法:取C57BL/6小鼠的脾细胞和骨髓细胞作为供体细胞;取18只CB6 F1小鼠(受体),随机分成对照组、环磷酰胺组和移植组,每组6只,皮下种植小鼠结肠癌CT26细胞建立荷瘤小鼠模型;然后分别于尾静脉注射0.2 mL生理盐水、环磷酰胺、环...     

白细胞介素11联合粒细胞集落刺激因子对小鼠异基因骨髓移植后移植物抗宿主病影响的研究

目的探讨重组白细胞介素11(rhIL-11)联合重组粒细胞集落刺激因子(rhG-CSF)用于供者对小鼠异基因骨髓移植后移植物抗宿主病(GVHD)的作用及其机制。方法建立同种异基因骨髓移植急性GVHD模型,以C57BL/6H-2b为供鼠,rhIL-11、rhG-CSF或二者联合应用作为动员剂,BALB/CH-2d受鼠经致死剂量(8·0Gy)照射后随机分为5组,A组为单纯照射组(10只),B组为GVHD对照组(18只),C组为G-CSF移植组(18只),D组为IL-11移植组(18只),E组为联合移植组(18只),在照射后4h移植供鼠骨髓细胞和脾细胞。观察移植后小鼠的生存期和病死率,应用流式细胞仪测定嵌合体形成情况,用四甲基偶氮唑盐(MTT)法检测混合淋巴细胞反应,酶联免疫吸附实验(ELISA)检测白细胞介素4(IL-4)、γ干扰素(IFN-γ)等细胞因子的分泌情况,并进行组间比较。结果E组小鼠生存时间明显长于其他各组(B、C、D),差异有统计学意义(P<0·01),30d存活率B、C、D、E组分别是:0、27·3%(3/11)、36·4%(4/11)、63·6%(7/11)。B组小鼠均出现GVHD病理改变,其程度与发生GVHD的时间密切相关,GVHD发生越早,病理改变越重;E组有3只小鼠在移植后20d内发生GVHD,病理程度改变较其他各组轻。与B、C、D组相比,E组移植后14d脾细胞培养上清中IFN-γ水平较低(92pg/ml±14pg/mlvs172pg/ml±31pg/ml、132pg/ml±23pg/ml、117pg/ml±28pg/ml,均P<0·01),IL-4的水平较高(36pg/ml±7pg/mlvs16pg/ml±4pg/ml、26pg/ml±4pg/ml、24pg/ml±4pg/ml,均P<0·05),D、E组肿瘤坏死因子α(TNF-α)水平相近,但明显低于B、C组(均P<0·01),并且E组对宿主抗原的淋巴细胞增殖均低于B、C、D组(0·22±0·08vs0·87±0·14、0·54±0·21、0·49±0·18,均P<0·01)。结论rhIL-11联合rhG-CSF用于供者能产生协同作用,减轻GVHD,其机制可能与受者体内T细胞向Th2细胞因子极化、炎症介质如TNF-α降低有关。     

Enhancing Effects of Ligustrazine on Expression of CD31 and Hematopoietic Reconstitution in Syngenic Bone Marrow Transplantation of Mice

Summary： The effect of ligustrazine on the expression of CD31 in syngenic bone marrow transplantation （BMT） mice was studied. Fifty-six Balb/c mice were divided into 3 groups： normal control group. BMT control group, and ligustrazine treated group. Syngenic BMT mouse models were established according to the literatures. In BMT control group and the ligustrazine treated group, the mice were given respecxively orally 0.2 mL saline and 2 mg ligustrazine twice a day. On the 7th, 14th, and 21st day after BMT, the mice were killed. The expression of CD31 on the surface of bone marrow nuclear cells （BMNC） was detected by flow cytometry. Peripheral blood leukocytes, platelets and BMNC were counted. Histological observation of bone marrow was made. The results showed thai in ligustrazine treated group the peripheral blood leukocylcs, platelets and BMNC counts, and the expression of CD31 on the day 7, 14, 21 after BMT were higher than in BMTcontrol group （P〈0.01 or P〈0.05）. In conclusion, ligustrazine could obviously enhance the CD31 expression on the surface of BMNC after syngcnic BMT in mice, which may be one of the mecha- nisms underlying the ligustrazine accelerating hematopoietic reconstitution in syngenic BMT.     

川芎嗪对骨髓移植小鼠造血细胞SDF-1表达水平的影响

为探讨川芎嗪对骨髓移植(BMT)后造血重建过程中基质细胞来源因子(SDF-1)的作用,建立了典型同基因BMT小鼠模型,胃饲川芎嗪注射液每次2 mg,2次/d,分别于BMT后第7、14 d计数外周血细胞骨髓单核细胞(BMNC)并测定骨髓组织切片中SDF-1的表达.结果表明川芎嗪组BMT后第7、14 d外周血细胞计数、BMNC计数及骨髓组织切片中SDF-1表达水平均高于BMT组.提示川芎嗪能在骨髓移植造血重建早期促进骨髓基质细胞表达SDF-1,可能是促进BMT后造血干细胞(HSC)回髓、加速造血重建的机制之一.