紫外可见分光光度法测定维生素B12含量

目的:探讨维生素B12片剂中维生素B12的含量测定方法.方法:紫外可见分光光度法.样品以标准曲线法为测定含量依据,在361 nm的波长处测定吸光度.结果:维生素B12在5 μg/mL～100 μg/mL浓度范围线性关系良好.回归方程Y=0.0193X 0.048,r=0.9937.结论:测定的5个不同批号样品其含量均在标示范围之内,该方法简便、准确、灵敏度高.     

维生素B12片含量测定样品处理方法的改进

目的:改进维生素B12片含量测定样品处理方法。方法:取维生素B12片55片,制成1 ml中含其约12.5μg溶液,采用紫外分光光度法测定吸光度A,检测波长为361 nm。结果:维生素B12在1.25～25.00μg/ml,具有良好的线性关系(r=0.999 9);最低检测浓度为1.25μg/ml;溶液稳定性良好,相对标准偏差为0.5%;标准方法测得含量结果分别为102.06%、102.35%和102.00%,改进方法测得含量结果分别为101.59%、101.86%和101.42%。结论:改进方法较标准方法减少了剥片量,降低了样品浓度,提高了检测准确性,缩短了分析时间,可用于维生素B12片含量测定。     

单纯形—紫外分光光度法测定复方维生素B注射液的含量

采用紫外分光光度法测定复方维生素B注射液四组分的含量。在222～290nm区间内,间隔2nm测定吸光度,实验数据用单纯形法进行计算。维生素B_1、B_2、B_6和烟酰胺的平均回收率分别为100.0%,99.95%,100.1%和100.0%。样品的成分含量计算值与药品标准测定值的平均相对误差为1.28%。该法可用于多组分复方制剂的含量测定计算。     

快速分离液相色谱法测定庆大霉素普鲁卡因维B12颗粒中维生素B12的含量

目的 建立快速分离液相色谱（RRLC）法测定庆大霉素普鲁卡因维B12颗粒中维生素B12含量的方法.方法 选用Agilent SB-C18柱（3.0 mm×150 mm,1.8 μm）;以0.005 mol·L-1磷酸二氢钾溶液（每1 000 mL加磷酸0.5 mL）-甲醇（75：25）为流动相;流速为0.5 mL·min-1;柱温40 ℃;检测波长361 nm;进样体积200 μL.结果 VB12在10～1 400 ng· mL-1浓度范围内与峰面积呈良好的线性关系（r=0.999 8,n=9）.平均加样回收率为100.2%（RSD为1.0%,n=6）.方法 的最低检出限为8.0 ng· mL-1.供试品溶液色谱图中VB12峰与相邻峰的分离度良好,样品中其他组分不干扰VB12的测定.结论 该方法快速,简便,重复性好,结果准确可靠,适用于庆大霉素普鲁卡因维B12颗粒中微量维生素B12的含量测定.     

紫外分光光度法测定辛伐他汀渗透泵型控释片的体外释放度

目的:建立用紫外分光光度法测定辛伐他汀渗透泵型控释片体外释放度的方法。方法:以5.0 g/L十二烷基硫酸钠水溶液为释放递质,转速100 r/min;递质温度37℃。根据《中华人民共和国药典》2005版二部附录XC转篮法操作,分别于0.5,1.0,1.5,2.0,3.0,4.0,6.0,8.0,12.0,24.0 h取样10 ml,及时补加同温度、同体积的相应递质。取续滤液稀释适当倍数,按照《中华人民共和国药典》2005版二部附录ⅣA分光光度法,在238 nm波长处测定吸光度,根据标准曲线计算不同时间样品液浓度,计算药物的累积释放百分率Q。结果:浓度在2.048~16.384μg/ml时,辛伐他汀-5.0 g/L十二烷基硫酸钠溶液的浓度与吸收度线性关系良好:c=16.591 0A-0.038 3(r=0.999 8),回收率与精密度良好。结论:紫外分光光度法可准确地测定辛伐他汀渗透泵型控释片的体外释放度。     

不同浸出条件对决明子紫外吸收光谱的影响

目的：探讨不同的浸出溶媒、保存温度、煎煮时间及配伍对决明子浸出液紫外吸收光谱的影响。方法：对不同浸出条件下的决明子浸液,用紫外可见分光光度计在190－400nm进行紫外扫描。结果：决明子的水浸液、醇水浸液在278nm处有一吸收峰;而乙醇浸液在此扫描范围无吸收峰,且在237－400nm的吸光度为0。决明子水浸液的室温保存比低温保存的吸光度小。决明子水浸液的吸光度,随着煎煮时间的延长而增加。与夏枯草、菊花和钩藤配伍后,决明子水浸液的紫外吸收光谱形状未发生变化,菊花使其吸光度有所降低,夏枯草和钩藤使其吸光度增加。与黄芩配伍后,决明子水浸液的紫外吸收光谱的形状发生了变化。在207,278,311nm处各有一吸收峰。结论：若决明子水浸液,醇水浸液,乙醇浸液中起降压作用的为同种物质,该物质在237－400nm的吸光度为0。决明子水浸液宜置于冰箱低温保存。从浸出速度和节省能源来考虑,决明子的煎煮时间不宜超过1h。菊花对决明子中化学成分的浸出有抑制作用,夏枯草,钩藤则对其有助溶作用。黄芩不仅影响决明子中化学成分的浸出浓度,而且影响某些化学成分的浸出形态。     

金银花中木犀草苷含量测定方法的耐用性考察

按照中国药典方法,利用反相高效液相色谱法,对分离条件的耐用性进行了研究。在不同的色谱条件下,研究了金银花中木犀草苷含量测定的色谱分离情况,结果当采用YMC-Pack ODS-A色谱柱,乙腈为流动相A,0.5%冰醋酸溶液为流动相B进行梯度洗脱,检测波长为350 nm,流速为1 mL/min,柱温为40 ℃时,分离效果较好,精密度和重复性良好,能达到中国药典相关要求。     

标准曲线法测定盐酸麻黄碱滴鼻液的含量

盐酸麻黄碱滴鼻液为医院制剂 ,2 0 0 0版中国药典[1] 收载的含量测定方法为紫外分光光度法中的标准对比法 ,即在同等条件下配制对照品溶液和样品溶液 ,在 5 70nm处测定吸光度后计算样品含量。此法虽较容量法、旋光法的灵敏度和精密度高 ,但操作较烦琐 ,消耗大。盐酸麻黄碱滴鼻液的生产特点为批次多批量小 ,为优化医院药检工作 ,我们对其进行了改进。本实验将中国药典中的对比法改为标准曲线法 ,既省去了每次配制麻黄碱对照液及茚三酮溶液之繁 ,又简化了检验操作 ,提高了工效 ,节约了试药 ,不失为医院药检工作的良方。1　仪器与试药UV - 2 1…     

双氢青蒿素的制备及其含量测定

青蒿素用硼氢化钾作还原剂 ,在一定条件下反应 ,生成双氢青蒿素。以双氢青蒿素对照品为指标 ,用紫外分光光度法测定样品中双氢青蒿素含量。经检测mP14 6～ 14 9℃ ,含量为 99.3 3 % ,平均收率为 5 7.93 %。认为标准溶液和样品溶液配制后 ,以放置 2h后摇匀再加减进行反应为宜 ;而从反应速度、反应稳定性和获得较大的吸光度来看 ,碱浓度以 2 %为佳。     

维生素B12注射液含量测定的改进

目的 建立维生素B12注射液的含量测定方法 .方法 采用高效液相色谱法,紫外检测器,C18柱,流动相分别为:甲醇-水(30-70)检测波长361nm,流速:1.0mL·min-1:进样体积:10μL,柱温:30℃.结果 维生素B12在21.2μg/mL～212μg/mL范围内与相应峰面积呈良好的线性关系r=0.9998(n=5)平均回收率99.7%(RSD=0.53%,n=6).方法 可靠、简便、专属性强、重现性好.可作为维生素B12注射液的含量测定方法.     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

Development on Adjustable Calibration Marker for Shock Wave Focus

Shock wave lithotripsy （SWL） is a treatment of choice for upper urinary stones. However, this procedure is inappropriate for obese patients because the focus is often unable to reach the target owing to the limited focal distance in shock wave source. Although treating such patients in a blast path may increase the application length of shock wave source, it＇s difficult to find this path on the lithotripter monitor. For this reason, we invented an adjustable calibration marker in order to set an effective focus in the shock wave hath.     

Study on the TCM Pathogenesis of Angiopathic Complications of Type II Diabetes

Excess production of reactive oxygen species(ROS)of mitochondrion mediated by hyperglycemia is the common pathogenesis of angiopathic complications of diabetes.TCM holds that the damp from the dysfunction of spleen.kidney and liver is the causative factor of complications of diabetes.This is similar to the mechanism of Ros resulting in angiopathic complications of diabetes.When the angiopathic complications of type II diabetes mellitus(T2DM)are difierentiated as caused by turbid damp in TCM can be explained as ROS.Since the obstruction of pathogenic damp in channels and collaterals is said to be the main pathogenesis,the treating principle should be dissolving the damp to remove the obstruction.     

Leptin, Ghrelin and TNF-α before and after Hypo-caloric Traditional Chinese Diet and Auricular Acupuncture

INTRODUCTION Obesity is a complex emergent problem, which can be possibly solved not only by the diet but also by the life style and promotion of a constant physical exercise. 1, 2 No doubt careful attentions must be given to the nutritional condition of obese people, the dietary habits, the somatic build (i.e. distribution of fat mass) and the organic functions linked to formation of the fat mass. All the parameters should be constantly monitored before, during and after a diet treatment. 3, 4, 5     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

Relationship between Dysglycemia and Carotid Atherosclerosis in Tibetan Population

People with dysglycemia are at high risk for atherosclerotic diseases. This study aims at investigating the atherosclerotic vascular damage in dysglycemia and its metabolic origin in Tibetan population.     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

CLINICAL SIGNIFICANCE OF SERUM CYTOKINES IL-1β,sIL-2R,IL-6,TNF-α,AND IFN-v IN ACUTE CORONARY SYNDROME

Objectives To explore serum cytokines levels (including IL-1 β, sIL-2R, IL-6, TNF-α, and IFN-v) and their significance in patients with acute coronary syndrome (ACS) and the subsequent follow-ups, with attempt to estimate the role of various serum inflammatory markers in the diagnosis and assessment of ACS.Methods The study population include 40 patients with acute myocardial infarction (AMI), 40 patients with unstable angina pectoris (UAP), and 40 controls. Among the 80 patients, 60 patients attended a follow up 4 months later. Serum inflammatory markers including IL-1 β, sIL-2R, IL-6, TNF-α, and IFN-v were measured by enzyme linked immunosorbent assay.Results Serum IL- 1 β, sIL-2R, IL-6, TNF-α were significantly higher in AMI group or UAP group compared to the control group and became significantly lower 4 months later in the follow-up patients. Serum levels of IFN-v shows no significant difference between AMI group or UAP group and controls, also showing no significant change when measured in follow up patients. There was no correlation between serum creatine kinase-MB isoenzyme levels and serum inflammatory markers either in UAP or AMI group. Furthermore, when divided into two subgroups using Wagner's QRS scoring system in the AMI group, there is no difference of each serum inflammatory marker between ≤ 6 scores group and ＞ 6 scores group.Conclusion Serum levels of certain inflammatory markers may have some diagnostic value for ACS, and can be a useful marker reflecting disease stability.     

Possible Spread of adenovirus type 3 from poultry to humans ：indirect evidence from an outbreak in China

Objective:To explore the epidemiology and etiology for an outbreak of acute respiratory tract infection that occurred in one county of Jiangsu Province, China 2004. Methods: Only cases meeting the case definition were included in the study. We reviewed the medical records of the cases who were admitted to the local hospitals, interviewed cases by a standard questionnaire, and then described the epidemiotogic features and analyzed risk factors by means of a case-control study. We collected pharyngeal swab specimens and sent them to different laboratories for isolation and culture. The laboratory used different detection methods such as DIP, PCR, electron microscope examination and microneutralization assay, to identify and then type the positive specimens. Results:A total of 871 cases were reported during the period from April 18 to July 4,2004. The distribution of onset times presented two peaks, one in late May and another in middle June. The epidemic occurred mainly in the elementary and junior high schools in ten townships of one county, and the mean age of the cases was 12 years (range 7 months to 18 years). The course of the disease was acute, and was characterized by fever accompanied with sore throat and tonsillitis. The WBC count of cases was normal or elevated. The mean duration of illness was 5 days (range 2 to 12 days). No fatalities from illness were reported. A case-control study indicated that the possible risk factors were close contact with a case and/or poultry before onset and sharing of towels among members of the family. The typical CPE was observed through inoculating pharyngeal swab specimens into the HEP-2 cell cultures in different laboratories. An infection of adenovirus type 3 was verified by detecting positive specimens in different methods. Conclusion:This investigation demonstrated that the acute respiratory infection in cases was caused by adenovirus type 3. Cases occurred in over 70 schools in ten townships in 2004, and the route of transmission was possibly close contact with cases or droplet transmission.