L—精氨酸对高胆固醇血症鹌鹑的降脂和抗脂质过氧化作用

本研究旨在观察Ｌ－精氨酸对高胆固醇血症鹌鹑主动脉弱杰硬化形成的抑制作用，取雄性日本鹌鹑３０只，分为３组，每组１０只。正常对照组喂基础饲料，高脂组喂１％胆固醇＋３％猪油；Ｌ－精氨酸组喂高脂饲料＋饮水补充２．２５％Ｌ－精氨酸。８周后处死动物，取出主动脉，形态学观察动脉粥样硬化病变和计算斑块面积百分比；获取血清作生化分析。     

L-精氨酸对高胆固醇血症鹌鹑主动脉粥样斑块面积的影响

为探讨Ｌ－精氨酸对高胆固醇血症鹌鹑主动脉粥样病变的消退作用。选择健康雄性日本鹌鹑８０只，分为正常对照组、高脂组、Ｌ－Ａｒｇ组和低脂组。前８周高脂饮食，复制动脉粥样硬化模型。在此基础上，Ｌ－Ａｒｇ组停喂胆固醇和油脂，饮水补充２．２５％Ｌ－精氨酸；低脂组恢复基础饲料，继续饲养８周。在８周末、１６周末，取出主动脉，形态学观察动脉粥样硬化病变；计算斑块面积百分比；收集血清作生化分析。（１）高脂组斑块面积从８周末的３７％上升到１６周末５０％，内膜从４０μｍ增厚到５６μｍ；（２）Ｌ－Ａｒｇ组主动脉斑块面积和内膜厚度明显减少；（３）补充２．２５％Ｌ－精氨酸８周，血清总胆固醇（ＴＣ）和丙二醛（ＭＤＡ）水平恢复正常，超氧化物歧化酶活性比高脂组上升８０％，比低脂组上升６０％。Ｌ－Ａｒｇ可能通过降血脂，减少脂质过氧化损伤而具有明显缩小实验性鹌鹑主动脉粥样硬化斑块面积的作用。     

L—精氨酸对高胆固醇血症鹌鹑主动脉粥样斑块面积的影响

为探讨Ｌ－精氨酸对高胆固醇血症鹌鹑主动脉粥样师变的消退作用。选择健康雄性日本鹌鹑８０分，分为正常对照组、高脂组、Ｌ－Ａｒｇ组和低脂组。前８周高脂饮食，复制动脉粥样硬化膜型。在此基础上，Ｌ－Ａｒｇ组停喂胆固醇和油脂，饮水补充２．２５％Ｌ－精氨酸；低脂组恢复基础饲料，继续饲养８周。在８周末、１６周末，取出主动脉，形态学观察动脉继样硬化病变；计算斑块面积百分比：收集血清作生化分析。（１）高脂组斑块面积从     

花椒挥发油对实验性动脉粥样硬化的影响

目的 观察中药花椒挥发油对豚鼠实验性主动脉粥样硬化形成的影响并探讨其可能的作用机制。方法 将36只豚鼠随机分为正常对照组、模型组、花椒挥发油组;分别给予普通饲料（正常对照组）和高脂饲料（模型组和花椒挥发油组）喂养,花椒挥发油组动物在喂高脂饲料的同时,加喂花椒挥发油10mg／kg。喂养第14周末取血样后,处死所有动物,检测血清脂质、载脂蛋白水平、血清丙二醛含量、肝脏和主动脉组织中总胆固醇和甘油三酯含量以及主动脉粥样硬化斑块面积。结果 高脂饲料喂养14周后,模型组和花椒挥发油组动物血脂水平均升高,且伴有主动脉粥样硬化斑块的形成;与模型组相比,花椒挥发油组动物血中低密度脂蛋白、低密度脂蛋白／高密度脂蛋白的比值、载脂蛋白B以及丙二醛水平降低,差异有统计学意义（P〈0．05）,而且肝脏和主动脉组织中总胆固醇和甘油三酯含量以及主动脉粥样斑块面积比均小于模型组,差异有统计学意义（P〈0．05）。结论 花椒挥发油具有抗豚鼠实验性动脉粥样硬化形成的作用;这种作用与它降低血清过氧化脂质水平、抗脂质过氧化损伤有关。     

L－精氨酸抗家兔动脉粥样硬化研究

在家兔高胆固醇血症模型中，观察升主动脉和主肺动脉粥样硬化病变及Ｌ，精氨酸抗粥样埂化作用。结果：（１）高胆固醇血症不仅诱发主动脉也诱发主肺动脉粥样硬化改变，其严重性二者相仿；（２）口服Ｌ－精氨酸使血浆游离精氨酸水平升高一倍而不影响血清胆固醇；（３）口服Ｌ－精氨酸有显著抗动脉粥样硬化作用，机理可能与其在代谢过程中使血管壁合成释放血管舒张因子（ＥＤＲＦ）、抑制某些促动脉粥样硬化发生因素有关。     

磁处理水对家兔实验性高LDL—C血症及主动脉内膜病变的影响

目的 探讨磁处理水对家兔实验性高低密度脂蛋白胆固醇（LDL－C）血症及主动脉内膜病变的影响。方法 将27只新西兰雄性大白兔随机平均分为对照（N）组、模型（AS）组和预防（MW）组,以高脂饲料喂养复制家兔动脉粥样硬化AS,MW组家兔喂高脂饲料同步软磁处理水。分阶段检测各组家兔血浆LDL－C含量,并观察其主动脉内膜病理学改变。结果 MW组血浆LDL－C水平明显较AS组较低（P＜0．01）,MW组主动脉内膜脂质沉积及粥样斑块形成面积较AS组减少（P＜0．01）,内膜增生程度较AS组减轻（P＜0．01）。结论 提示磁处理水在家兔实验性高LDL－C血症及AS的预防中起一定作用。     

L—精氨酸抗家兔动脉粥样硬化研究

在家兔高胆固醇血症模型中，观察升主动脉和主肺动脉粥样硬化病变及Ｌ－精氨酸抗粥样硬化作用。结果：１高胆固醇血症不仅诱发主动脉也诱发生肺动脉粥样硬化改变，其严重性二者相仿；２口服Ｌ－精氨酸使血浆游离精氨酸水平升高一倍而不影响血清胆固醇；３口服Ｌ－精氨酸有显著抗动脉粥样硬化作用，机理可能与其在代谢过程中使血管壁合成释放血管舒张因子，抑制某些促动脉粥样硬化发生因素有关。     

脑脉舒宁对大鼠血脂血浆粘度的影响

采用脑脉舒宁提取液（1ml相当于生药0．5g）以50％和25％浓度分别对两组食高脂饲料的大鼠进行灌胃，对照组为生理盐水。每次10．0ml／kg，每日1次，连用10天，于实验第11天对3组大鼠进行断头取血。测定其血胆固醇、甘油三酯和血浆粘度。结果显示：高浓度（50％）给药组大鼠的血胆固醇、甘油三酯和血浆粘度均下降，与对照组比较有显著性差异（P＜0．05和P＜0．01）；而低浓度（25％）给药组大鼠仅血浆粘度显著下降（P＜0．01）。给药前后各级大鼠的体重、饮水和饮食量无明显差异。每组大鼠胸主动脉和冠状动脉病理切片无粥样硬化病变产生。     

水飞蓟油对兔实验主动脉粥样硬化病变影响的观察

水飞蓟种籽含油量在３０％以上含亚油酸为５３．６５％。水飞蓟油有降低血浆胆固醇和治疗高血压病作用。本实验证明水飞蓟油防治实验性主动脉粥样硬化有显著作用；胆固醇组主动脉粥样硬化斑块面积总和为５７．０７ｃｍ＾２，水飞蓟油组为４５．７ｃｍ＾２。硬化斑块占主动脉内膜总面积：胆固醇组为３４．２０％，水飞蓟油组为３０．５２％，二组间存在差显著差异（Ｐ＜０．０５）。实验性主动脉粥样硬化斑块分级的积分值分别是对照组     

家兔动脉粥样硬化模型稳定性的观察

目的观察用饲喂法制造的家兔动脉粥样硬化模型，停喂高脂饲料后是否可以恢复正常。方法试验分3组，分别饲喂3种含不同量胆固醇、猪油及蛋黄的饲料，数周后测其血清、检查主动脉，待主动脉形成动脉粥样硬化后，停喂高脂饲料，观察主动脉变化情况。结果饲喂11周的家兔主动脉壁内膜布满脂质斑块，形成动脉粥样硬化。停喂高脂饲料15周仍呈现主动脉粥样硬化。结论饲喂法制造的家兔动脉粥样硬化模型，停喂高脂饲料15周内未恢复正常，说明家兔动脉粥样硬化模型在一定时间内是稳定的。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

Development on Adjustable Calibration Marker for Shock Wave Focus

Shock wave lithotripsy （SWL） is a treatment of choice for upper urinary stones. However, this procedure is inappropriate for obese patients because the focus is often unable to reach the target owing to the limited focal distance in shock wave source. Although treating such patients in a blast path may increase the application length of shock wave source, it＇s difficult to find this path on the lithotripter monitor. For this reason, we invented an adjustable calibration marker in order to set an effective focus in the shock wave hath.     

Study on the TCM Pathogenesis of Angiopathic Complications of Type II Diabetes

Excess production of reactive oxygen species(ROS)of mitochondrion mediated by hyperglycemia is the common pathogenesis of angiopathic complications of diabetes.TCM holds that the damp from the dysfunction of spleen.kidney and liver is the causative factor of complications of diabetes.This is similar to the mechanism of Ros resulting in angiopathic complications of diabetes.When the angiopathic complications of type II diabetes mellitus(T2DM)are difierentiated as caused by turbid damp in TCM can be explained as ROS.Since the obstruction of pathogenic damp in channels and collaterals is said to be the main pathogenesis,the treating principle should be dissolving the damp to remove the obstruction.     

Leptin, Ghrelin and TNF-α before and after Hypo-caloric Traditional Chinese Diet and Auricular Acupuncture

INTRODUCTION Obesity is a complex emergent problem, which can be possibly solved not only by the diet but also by the life style and promotion of a constant physical exercise. 1, 2 No doubt careful attentions must be given to the nutritional condition of obese people, the dietary habits, the somatic build (i.e. distribution of fat mass) and the organic functions linked to formation of the fat mass. All the parameters should be constantly monitored before, during and after a diet treatment. 3, 4, 5     

Relationship between Dysglycemia and Carotid Atherosclerosis in Tibetan Population

People with dysglycemia are at high risk for atherosclerotic diseases. This study aims at investigating the atherosclerotic vascular damage in dysglycemia and its metabolic origin in Tibetan population.     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.