蒙药玉簪花中山奈酚的HPLC含量测定与薄层色谱鉴别

目的：建立蒙药玉簪花中山奈酚的薄层色谱鉴别与含量测定方法。方法：采用薄层色谱法定性鉴别蒙药玉簪花中黄酮类成分山奈酚;采用HPLC法,ODS-C18分析柱（250mm×4.6？,5μm）;流动相为甲醇-0.4%磷酸（67∶33）;流速1mL/min,检测波长360 nm。结果：薄层鉴别方法分离度良好;HPLC含量测定法中山奈酚的线性范围为0.37～2.2μg（r=1.0000）;平均回收率为96.69%（RSD=1.34%,n=6）。结论：所建立的方法简便、准确、重复性好,可以作为蒙药玉簪花中山奈酚的定性及定量方法。玉簪花中山萘酚的含量依产地、生长期、入药部位不同而不同。     

RP-HPLC法测定六味地黄汤生物制剂中丹皮酚的含量

目的建立测定六味地黄汤生物制剂中丹皮酚含量的方法。方法采用反相高效液相色谱法：色谱柱为Uhimate^TMAQ—C18色谱柱（4．6mm×250mm,5μm）,以甲醇-水（体积比45：55）为流动相,流速1mL／min,柱温30℃,检测波长274nm。结果丹皮酚的质量浓度在1．024～25．60μg／mL范围内与峰面积线性关系良好（r=0．9990,n＝5）,平均回收率103．3％,RSD为1．5％。结论该方法准确、专属性强、重复性好,可用于测定六味地黄汤生物制剂中丹皮酚的含量。     

岩白菜质量标准研究

目的：建立岩白菜中有效成分岩白菜素的HPLC测定方法,测定其含量。方法：采用反相HPLC法,色谱柱为ZORBAX Eclipse×DB—C18柱（250mm×4．6mm,5μm）,流动相为甲醇：水（30：70）,流量1．0mL／min,柱温30℃。结果：岩白菜素在0．1068μg－0．5340μg范围内具有良好的线性关系（r＝0．9998）,平均加样回收率为98．6％,RSD=0．5％。结论：该方法操作简单、准确、重现性好,可用于岩白菜药材的含量测定。     

HPLC同时测定四正颗粒中厚朴酚、和厚朴酚含量

目的：建立HPLC同时测定四正颗粒中厚朴酚、和厚朴酚的含量测定方法。方法：采用Kromasil ODS—C18柱（4．6mm×250mm,5μm）,流动相甲醇－异丙醇－1％磷酸溶液（36：21：40）,流速1．0mL／min,检测波长为294nm,柱温35℃。结果：厚朴酚在0．2616-1．5696μg（r=0．9999）,和厚朴酚在0．1282-0．7692μg（r=0．9999）呈良好的线性关系,平均回收率厚朴酚为99．01％,RSD为1．37％（n=6）,和厚朴酚为99．02％,RSD为1．41％（n=6）。结论：本法准确可靠,重复性好,可用于四正颗粒的质量控制。     

HPLC 法测定太平糖克丸中马钱苷的含量

目的：建立太平糖克丸中马钱苷的含量测定方法。方法：采用高效液相色谱法,Prontosil 120－5－C18柱（250mm ×4.6mm,5μm）,流动相为四氢呋喃－乙腈－甲醇－0．05％磷酸溶液（1∶8∶4∶87）;流速1．0mL／min;检测波长236nm;柱温30℃;进样量10μL。理论板数按马钱苷峰计算应不得低于4000。结果：马钱苷对照品进样量在0．0302 g～3．0177 g范围内,进样量与峰面积呈良好的线性关系（r＝0．9999）,马钱苷平均加样回收率为97．24％,RSD值1．55％（n＝6）。结论：本方法操作简便,准确可靠,专属性强,重现性好。适宜用于测定太平糖克丸中马钱苷的含量。     

HPLC法测定回春散中大黄素和大黄酚的含量

目的：建立测定回春散中大黄素和大黄酚的含量的方法。方法：采用高效液相色谱法。以C18柱为色谱柱，以ψ（甲醇：0．2％磷酸）＝76：24为流动相，检测波长为254nm。结果：大黄素在0．02－0．20μg，大黄酚在0．04－0．40μg 内呈良好线性关系，加样回收率分别为97．42％，99．07％，结论：本法简便，准确，可用于回春散的质量控制。     

超高效液相色谱-质谱联用法测定川楝子中川楝素的含量

目的：建立测定楝科植物川楝子中川楝素的超高效液相色谱质谱（UPLC - MS）方法。方法采用 XDB - C18色谱柱（2.1 mm ×150 mm,3.5μm）,流动相为乙腈—水（含0.1％甲酸）＝35：65（V： V）,流速为0.3 ml/ min,柱温30℃。结果方法的线性范围为0.1~300μg/ ml（R2＝0.9997）,平均加样回收率﹥90％,相对标准偏差（RSD）为﹤1.66％。结论该方法灵敏度高、分析时间短、提取回收率高、重现性好,适用于川楝子中川楝素含量测定。     

桑叶中芦丁含量的动态变化研究

目的：研究不同时期桑叶中芦丁的含量。方法 Kromasil C18柱（4.6 mm ×250 mm,5μm）;流动相：甲醇-0.5％磷酸水溶液（梯度洗脱）,检测波长：358 nm;柱温：30℃;结果芦丁在0.1262~2.02μg范围内有良好的线性关系,r＝0.9999,平均回收率为97.88％,桑叶中芦丁含量最高在霜降前后。结论初步表明了桑叶中芦丁含量随季节变化的规律。     

复方苦参碱注射液中苦参碱和氧化苦参碱的总含量测定

目的：建立复方苦参碱注射液中苦参碱和氧化苦参碱总含量的测定方法。方法：采用高效液相色谱法,用氨基键合硅胶填充柱（150×4．6 mm,5μm）;流动相：乙腈-无水乙醇-（体积分数）3％磷酸溶液（801010）;流速：1．0 mL·min －1;检测波长：220 nm;柱温：30℃;进样量10μL。结果：苦参碱在0．17～2．08μg 范围内呈良好的线性关系（r ＝0．9998）,氧化苦参碱在0．38～4．59μg 范围内呈良好的线性关系（r ＝0．9997）,平均回收率为97．86％（n ＝6）,相对标准偏差 RSD 为1．53％。结论：该方法操作简单,准确度高,重复性好,可用于复方苦参碱注射液中苦参碱和氧化苦参碱总含量的测定。     

HPLC法测定人参皂苷Rg3、Rh1的含量

目的：建立人参发酵品中人参皂苷Rh1、Rg3的含量测定方法。方法采用高效液相色谱法测定,色谱条件：ODS色谱柱（4.6 mm ×250 mm,5μm）;检测波长203 nm;流动相（Rh1）：乙腈-水（26.5：73.5）,流动相（Rg3）：乙腈-0.05％磷酸（37：63）;柱温30℃,流速1.0 mL/min。结果人参皂苷Rh1的含量测定线性范围0.1~1.1μm,相关系数r＝0.9998,平均加样回收率96.90％,RSD 2.67％。人参皂苷Rg3的含量测定线性范围0.4~3.0μm,相关系数为r＝0.9997,平均加样回收率98.56％,RSD 2.51％。结论本方法检测灵敏度高,检测结果可靠。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

Development on Adjustable Calibration Marker for Shock Wave Focus

Shock wave lithotripsy （SWL） is a treatment of choice for upper urinary stones. However, this procedure is inappropriate for obese patients because the focus is often unable to reach the target owing to the limited focal distance in shock wave source. Although treating such patients in a blast path may increase the application length of shock wave source, it＇s difficult to find this path on the lithotripter monitor. For this reason, we invented an adjustable calibration marker in order to set an effective focus in the shock wave hath.     

Study on the TCM Pathogenesis of Angiopathic Complications of Type II Diabetes

Excess production of reactive oxygen species(ROS)of mitochondrion mediated by hyperglycemia is the common pathogenesis of angiopathic complications of diabetes.TCM holds that the damp from the dysfunction of spleen.kidney and liver is the causative factor of complications of diabetes.This is similar to the mechanism of Ros resulting in angiopathic complications of diabetes.When the angiopathic complications of type II diabetes mellitus(T2DM)are difierentiated as caused by turbid damp in TCM can be explained as ROS.Since the obstruction of pathogenic damp in channels and collaterals is said to be the main pathogenesis,the treating principle should be dissolving the damp to remove the obstruction.     

Leptin, Ghrelin and TNF-α before and after Hypo-caloric Traditional Chinese Diet and Auricular Acupuncture

INTRODUCTION Obesity is a complex emergent problem, which can be possibly solved not only by the diet but also by the life style and promotion of a constant physical exercise. 1, 2 No doubt careful attentions must be given to the nutritional condition of obese people, the dietary habits, the somatic build (i.e. distribution of fat mass) and the organic functions linked to formation of the fat mass. All the parameters should be constantly monitored before, during and after a diet treatment. 3, 4, 5     

Relationship between Dysglycemia and Carotid Atherosclerosis in Tibetan Population

People with dysglycemia are at high risk for atherosclerotic diseases. This study aims at investigating the atherosclerotic vascular damage in dysglycemia and its metabolic origin in Tibetan population.     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.