新生儿烧伤创面治疗的临床探讨

新生儿烧伤较为少见 ,我科于 1 981年— 1 996年 1 6年共收治 9例 ,现对其创面的早期切痂植皮和保守治疗的愈合效果进行初步的比较观察。材料与方法1　一般资料　本组男 6例 ,女 3例 ,受伤年龄由出生至 1 0天 ,平均 (3 .45± 4.2 4)天 ;烧伤总面积 3 %～1 5 % ,平均 6.5 6%± 4.2 6% ;Ⅲ 面积 0～ 1 5 %。平均 4.89%±4.46% ;早期切痂植皮 4例 ,保守换药 ,自然脱痂后肉芽创面植皮 5例 ;伤情及治疗情况见表 1。表 1　伤　情　及　治　疗　情　况病例 性别 年龄 受　伤原　因 伤后入院时间 烧　伤部　位 烧伤面积 (% )总/Ⅲ /深Ⅱ 伤后手术时…     

碱烧伤创面切痂与植皮时机的选择(附60例报告)

目的:回顾性分析几种碱烧伤创面的修复方法,探讨手术切痂与植皮时机,提高植皮成活率。方法:对60例碱烧伤患者176处创面(浅Ⅱ°26,深Ⅱ°60,Ⅲ°90)治疗进行分析。26处浅Ⅱ°和60处深Ⅱ°创面应用保痂疗法;90处Ⅲ°创面其中的52处用传统方法(3～7d)切痂植皮;另外38处伤后3～36h切痂,延迟48h植皮。结果:26处浅Ⅱ°创面14～16d自愈;60处深Ⅱ°创面28～56d后行自体皮移植封闭创面,愈合创面皮肤薄,疤痕重,痛痒明显;90处Ⅲ°创面其中52处传统切痂植皮,术后18～25d愈合,植皮早期皮片呈花斑状,粘附差,部分表皮游离,后期需补植自体皮方能封闭创面;另38处Ⅲ°创面行早期切痂后异体皮或生物敷料覆盖,延迟48h植皮,植皮早期皮片颜色红润,粘附好,表皮无游离现象,术后7～14d愈合,愈合皮肤接近正常皮肤,后期疤痕轻,和传统手术方法比较植皮成活率高,创面愈合快,平均愈合时间提前8～10d。结论:碱烧伤创面深Ⅱ°和Ⅲ°同样需要手术治疗;手术治疗应采取切痂延迟植皮的方法,虽然为两次手术,但每次手术时间短,出血少,植皮成活率高。     

早期削痂复合皮混合移植治疗颜面部深度烧伤

 【目的】探讨颜面部深度烧伤创面早期削痂复合皮混合移植的治疗方法及效果。【方法】对19例颜面部深度烧伤患者在伤后1d内行削痂术，同时一期混合移植异体脱细胞真皮基质和大张自体刃厚皮片。术后10～12d打开敷料．观察植皮效果，随访3月～24月。【结果】本组患者复合皮移植成活率90％，少部分移植的表皮出现水疱，经水胶体敷料覆盖3～5d后即可愈合。随访移植后面部皮肤外观丰满，弹性好，表情丰富，远期效果满意。【结论】面部深度烧伤创面早期削痂复合皮混合移植可以取得满意效果。     

早期磨痂加异种脱细胞真皮覆盖治疗面部深Ⅱ度烧伤创面48例

目的:观察颜面部深Ⅱ度烧伤创面早期磨痂术加异种脱细胞真皮覆盖的效果.方法:单纯颜面部烧伤和全身烧伤包括颜面部烧伤48例.颜面部创面于伤后1～3 d内,在静脉复合麻醉或基础麻醉加局部麻醉下,用消毒钢丝球和电动磨削机磨去创面坏死组织,以基底泛红或有针尖状出血为宜,然后用异种脱细胞真皮基质覆盖,观察创面愈合时间及瘢痕增生情况.创面愈合后1年随访观察.结果:本组48例,创面愈合时间为12～19 d,平均(15±2) d.其中45例肤色正常,皮肤弹性好,无瘢痕;2例出现花斑样色素沉着;1例出现轻度瘢痕增生.结论:早期磨痂能有效地清除创面坏死组织,尽量保留有生机组织;脱细胞真皮基质无抗原性,透气不易积液,不需换药;创面愈合快,瘢痕增生少,值得推广引用.     

早期削痂复合皮混合移植治疗颜面部深度烧伤

【目的】探讨颜面部深度烧伤创面早期削痂复合皮混合移植的治疗方法及效果。【方法】对19例颜面部深度烧伤患者在伤后1d内行削痂术，同时一期混合移植异体脱细胞真皮基质和大张自体刃厚皮片。术后10～12d打开敷料．观察植皮效果，随访3月～24月。【结果】本组患者复合皮移植成活率90％，少部分移植的表皮出现水疱，经水胶体敷料覆盖3～5d后即可愈合。随访移植后面部皮肤外观丰满，弹性好，表情丰富，远期效果满意。【结论】面部深度烧伤创面早期削痂复合皮混合移植可以取得满意效果。     

早期切痂植皮术治疗颜面深度烧伤

颜面部器官因其暴露而易被烧伤 ,发生率 5 4 % ,修复较为困难。 1990— 2 0 0 3年我科分别采用早期切痂或中晚期肉芽创面刮除分区大张厚断层植皮二种术式治疗全颜面深度烧伤 32例 ,疗效较好 ,现报告如下。1　临床资料1.1.　一般资料　早期切痂或中晚期肉芽创面刮除分区植皮32例 ,其中早期切痂植皮组 2 1例 ,男 14例 ,女 7例 ,年龄 16～ 4 4岁 ,烧伤总面积 6 %～ 72 % ,三度烧伤面积 1%～ 5 1%。中晚期肉芽创面组 11例 ,男 8例 ,女 3例 ,年龄 2 1～ 6 1岁 ,烧伤总面积 4 %～ 90 % ,三度烧伤面积 1%～ 6 3%。手术时间 :切痂组在伤后 4～ 12d进…     

小儿Ⅲ~。烧伤早期手术治疗

小儿烧伤占整个住院烧伤病人的49％,76年前20年,我院对所有的Ⅱ°烧伤患儿,不论其面积大小,惯用蚕食脱痂植皮,以消灭创面。长期临床观察发现、蚕食脱痂植皮病程长,败血症发生率高,愈合后瘢痕挛缩严重。76年后我们对10例小儿Ⅱ°烧伤,试行早期切痂,取得良好效果。     

23例大面积烧伤治疗的体会

<正> 自1988年至1993年12月,共收治各类烧伤病人196例,面积超过50％者23例,最大面积％,全部治愈。23例中,男性17例,女性6例,儿童例(最小2个月)。烧伤创面含Ⅲ°者18例,入院时有休克者16例,合并吸入性损伤3例.入院清创后创面用中药结痂酊取暴露治疗,浅Ⅱ°7～15天,深Ⅱ°21～30天痂下愈合,5例因感染加深成Ⅲ°经肉芽创面植皮痊愈.含Ⅲ°创面18例(10.1～23.9％),其中12例蚕蚀脱痂植皮,6例分别在伤后6～12天切痂植皮,皮片成活率均在90％以     

碱烧伤932例治疗体会

目的 总结十年来我院932例碱烧伤患者的诊治,探讨碱烧伤的治疗方法.方法 入院后,烧伤面积小、伤情轻者先冲洗半小时以上后清创;烧伤面积较大、病情危重者在平稳抗休克基础上冲洗清创,然后根据情况区别对待.浅Ⅱ度创面采用包扎或暴露疗法并及时观察:如果创面加深尽早削痂植皮,已明确的小面积深Ⅱ度及Ⅲ度创面可即时削痂植皮,大面积重症患者则在休克期或过后及早分次削、切痂植皮,削痂深度以pH试纸不变蓝为止.结果 单纯浅Ⅱ度烧伤407例,经磺胺嘧啶银(锌)包扎换药治疗或保痂治疗痊愈,愈合时间2～3周,平均16 d,其中包扎治疗愈合时间短,优于保痂治疗.总面积<50%体表总面积(TBSA)、深Ⅱ度和Ⅲ度<20%的患者502例,痊愈时间28～65 d,平均42 d;总面积>50%TBSA,其中深Ⅱ度和Ⅲ度以上烧伤>20%的患者23例,痊愈时间68～156 d,平均96 d.结论 碱烧伤后第一时间用清水冲洗半小时以上,可以减轻碱烧伤的程度;即时、休克期或伤后4～7 d削切痂植皮的削切痂深度至pH 7.0左右的正常组织,然后用大量生理盐水冲洗后一期植皮,则植皮成活率可大大提高;加强安全防护教育,采取有效的防护措施可以减少碱烧伤的发生.     

早期磨痂治疗全颜面部深Ⅱ°烧伤临床体会

颜面部因其部位暴露,遭受烧伤机会多。创面早期处理好坏与容貌、功能恢复密切相关。既往报道对颜面部深Ⅱ。烧伤多采用早期削痂植皮,愈后瘢痕重、挛缩畸形明显。自2000 年以来,采用伤后24 h内对颜面部深Ⅱ。烧伤创面进行磨痂,收到良好效果。     

Evaluation of diuretic and laxative activity of hydro-alcoholic extract of Desmostachya bipinnata （L,） Stapf in rats

OBJECTIVE： In continuation to the growing evidence for therapeutical potential of Desmostachya bipinnata （Linn） Stapf, the current pharmacological study was carried out to evaluate the diuretic and laxative activity of its hydro-alcoholic extract in rats. METHODS： The hydro-alcoholic extract of D. bipinnata whole plant was prepared by using Sox- hlet extractor and subjected to analysis by standard preliminary phytochemical tests. Evaluation of both diuretic and laxative activity was carried out using standard methods as reported earlier. Frusemide （20 mg/kg） was served as positive control for diuretic activity and sennosides （10 mg/kg） served as negative control for laxative activity. RESULTS： The hydro-alcoholic extract showed significant diuretic activity and was found to be the most potent in increasing the urinary output at 500 mg/kg when the effect was compared with that of the standard frusemide （P〈0.01）. Moreover, this extract was found to be most effective in increasing urinary electrolyte concentration （Na＋, K＋, and Cl-） at both doses tested. Whereas the results for laxative activity showed minimal increase of feces output at the dose of 500 mg/kg and the increase was negligible when compared with that of the standard drug sennosides. CONCLUSION： Altogether, the above significant findings validate and support its folkloric diuretic use and lend pharmacological credence to the ethno-medical use of this plant in traditional system of medicine, which demands further studies to investigate its active constituents, as well as its use and safety.     

New Mechanism of Herb-herb Interaction between Ginseng and Trogopterus Based on CYPs in Rat Livers

The study was designed to investigate the potential mechanism of herb-herb interaction between ginseng and Trogopterus （Trg） based on Cytochrome P450 isozymes （CYPs） in rat livers. We estimated the influence on CYP1A2, CYP2E1, and CYP3A1/2 activity caused by ginseng and Trg used in combination. The CYP1A2 and CYP2E1 enzyme activity were induced by ginseng and Trg used in combination. And this induction effect was caused via inducing CYP1A2 and CYP2E1 protein expression which was supposed caused by inducing the gene expression of CYP1A2 and CYP2E1.     

UniPron is A Fully Effective Non-hormonal Reversible Contraceptive in Baboon Model （Papio Anubis）

Objective To determine the safety and efficacy of UniPron as a reversible contraceptive.
Methods Vaginal swabs were obtained before and after UniPron administration, cultured onto appropriate culture media and bacteria identification was done based on type of media used, Gram stain reactions, colony morphology and biochemical tests. Vaginal biopsy tissues were processed using paraffin wax method, stained with hematoxylin and eosin and examined under light microscopy to determine the effect of the product on vaginal tissues. The effect of UniPron on sperm was examined by mixing the product with electroejaculated spermatozoa in vitro at different concentrations. For efficacy studies, male baboons of proven fertility were mated with UniPron treated or untreated females of proven fertility during the fertile stages.
Results All the five females （100%） that were treated with UniPron did not conceive and they regained total fertility when the treatment was stopped while all the controls conceived. At a concentration of 40%, UniPron completely immobilized spermatozoa in an in-vitro system. UniPron mechanism of action was by lowering the vaginal pH and on application in baboon, the pH was lowered for at least 3 h after which it went back to normal.
Conclusions As we plan for a study to test UniPron as a microbicide to prevent STIs including HIV, our current study has established that this novel product is effective in contraception and harmless to vaginal tissues and vaginal microbial flora in a baboon model （Papio anubis）.     

表皮生长因子受体酪氨酸激酶抑制剂在非小细胞肺癌应用的瓶颈——获得性耐药

以表皮生长因子受体酪氨酸激酶抑制剂（epidermal growth factor receptor-tyrosine kinase inhibitor,EGFR-TKI）分子靶向治疗研究为肇始,转化性研究使基础实验和临床实践间的鸿沟迅速填平,改变着人们认识治疗肺癌的视角。无可否认,EGFR-TKI上市后极大地延伸了肿瘤学家治疗肺癌的手段,但不管从临床经验、临床研究数据、分子生物学层面还是文献计量学的角度,     

Effect of the disruption of three cytoskeleton components on chondrocyte metabolism in rabbit knee cartilage

Background Chondrocytes＇ phenotype and biosynthesis of matrix are dependent on having an intact cytoskeletal structure.Microfilaments,microtubules,and intermediate filaments are three important components of the cytoskeletal structure of chondrocytes.The aims of this study were to determine and compare the effects of the disruption of these three cytoskeletal elements on the apoptosis and matrix synthesis by rabbit knee chondrocytes in vitro.Methods Chondrocytes were isolated from full-thickness knee cartilage of two-month-old rabbits using enzymatic methods （n=24）.The isolated cells were stabilized for three days and then exposed to low,medium,and high doses of chemical agents that disrupt the three principal cytoskeletal elements of interest：colchicine for microtubules,acrylamide for intermediate filaments,and cytochalasin D for actin microfilaments.A group of control cells were treated with carrier.Early apoptosis was assessed using the Annexin-FITC binding assay by flow cytometry on days 1 and 2 after exposure to the disrupting chemical agents.The components and distribution of the cytoskeleton within the cells were analyzed by laser scanning confocal microscopy （LSCM） with immunofluorescence staining on day 3.The mRNA levels of aggrecan （AGG） and type Ⅱ collagen （Col-2） and their levels in culture medium were analyzed using real-time PCR and enzymelinked immunosorbent serologic assay （ELISA） on days 3,6,and 9.Results In the initial drug-dose-response study,there was no significant difference in the vitality of cells treated with 0.1 μmol/L colchicine,2.5 mmol/L acrylamide,and 10 μg/L cytochalasin D for two days when compared with the control group of cells.The concentrations of colchicine and acrylamide treatment selected above significantly decreased the number of viable cells over the nine-day culture and disrupted significantly more cell nuclei.Real-time PCR and ELISA results showed that the mRNA levels and medium concentrations of AGG and Col-2 were significantly decreased for     

Liuwei Dihuang,a traditional Chinese herbal formula,suppresses chronic inflammation and oxidative stress in obese rats

OBJECTIVE： To investigate the anti-inflammatory, anti-oxidative stress, and adipokine-ameliorating effects of Liuwei Dihuang （LWDH）, a traditional Chinese herbal formula, in obese rats. METHODS： After 2 weeks of acclimation with free access to regular rodent chow and water, obese-prone-caesarean-derived （OP-CD） rats were fed a modified AIN-93G diet containing 60% energy from fat. Treatment was performed twice daily by gavage feeding with 500, 1 500, or 3 500 mg/kg body weight LWDH suspended in water （n=12 rats per group）. Twelve obese-resistant-CD （OR-CD） rats were fed the atherogenic diet and gavaged with water, and served as the normal control. Blood biomarkers of inflammation, oxidative stress and adiponectin were measured post-sacrifice and used to determine the treatment effect of LWDH and assess the suitability of OR/OP-CD rats for studying these parameters. RESULTS： After 9 weeks of treatment, LWDH lowered serum C-reactive protein （CRP） and tumour necrosis factor-α （TNF-α） levels. Serum interleukin-6 （IL-6） levels showed a tendency towards reduction, but were not significantly different from the OP-CD control. Liver superoxide dismutase （SOD） activity was increased in response to all three doses of LWDH, while the levels of reduced （GSH） and oxidized glutathione （GSSG） and thiobarbituric acid reactive substances （TBARS） were unchanged. Serum adiponectin levels were increased in response to oral administration of LWDH at the dose of either 500 or 1 500 mg/kg body weight. In addition, comparisons between OR-CD and OP-CD rats revealed differential, and for some biomarkers, conflicting characteristics of high-fat diet-fed OP-CD rats in reference to obese human subjects in terms of inflammatory and oxidative stress biomarkers and circulating adiponectin levels. CONCLUSION： The results show, for the first time, the anti-inflammatory, anti-oxidative stress and adiponectin-ameliorating effects of LWDH in obese rats. The suitability of the OP-JOP-CD rat model as     

益智健脑颗粒联合针灸对阿尔茨海默病大鼠学习记忆的影响

目的观察益智健脑颗粒联合针灸对阿尔茨海默病（Alzheimers disease,AD）大鼠学习记忆的影响。方法将大鼠随机分为假手术组（A组）、模型组（B组）、针灸组（C组）、益智＋针灸组（D组）各10只,B、C、D 3组分别以海马CA1区注射β淀粉样蛋白25-35（Aβ25-35）造模,A组注射等量的双蒸水,各组分别治疗20 d后行Morris水迷宫试验,观察大鼠学习记忆能力变化。结果B组较A组的平均潜伏期明显延长,差异具有统计学意义（P〈0.05）;与B组比较,C组、D组的平均潜伏期明显缩短,过台次数增多,差异具有统计学意义（P〈0.05,P〈0.01）;与C组比较,D组的潜伏期缩短,过台次数增多,差异具有统计学意义（P〈0.05）。结论益智健脑颗粒联合针灸能够提高Aβ25-35介导的AD模型大鼠的学习记忆能力。     

The Targeted Pro-apoptotic Effect of scFv-mediated Delivery of Caspase-6 on HER2-positive Osteosarcoma Cells

Objective To investigate the pro-apoptotic effect of scFv-mediated delivery of caspase-6 fusion protein on osteosarcoma E 10 cells. Methods The expression of human epidermal growth factor receptor-2 （Her-2） in osteosarcoma cell line E10 was examined by flow cytometry analysis. ScFv-mediated delivery of caspase-6 was generated by sequential fusion of the genes of a signal peptide, a single-chain Her-2 antibody （e23sFv）, a PEA translocation domain （PEA aa253-264）, and an recombinant caspase-6. The pCMV-immunocaspase-6 was produced by cloning above mentioned fusion genes into pCMV plasmid, and then pCMV-immunocaspase-6 plasmid mixed cationic liposome was transfected into E10 cells. The expression of fusion gene on transfected E10 cells was detected by immunocytochemical staining, The pro-apoptotic effects of fusion gene expression on morphology and growth status of E10 cells were observed by trypan blue cell staining, electron microscopy and MTT assay. Results The fusion protein was detected in the cytoplasm of the transfected E10 cells. These cells presented typical characteristics of apoptosis as detected by electron microscopy. MTT assay revealed that the proliferation of transfected E10 cells was suppressed than that of non- or mock-transfected cells （P〈0.01）. Conclusion ScFv-mediated delivery of caspase-6 fusion protein can induce apoptosis of Her-2 positive osteosarcoma E10 cells, suggesting the potential of this strategy for the treatment of human high-grade osteosarcoma.     

WHO西太区与“世界中医药学会联合会”中医名词术语国际标准比较研究：阴阳学说部分

《WHO西太区传统医学国际标准名词术语》中收录有关阴阳学说及其相关概念的术语有16条，“世界中医药学会联合会”（以下简称世中联）标准《中医基本名词术语中英对照国际标准》中收录了39条，其翻译基本与现行译法一致，如将“阴中之阴”译作yin within yin，将“阴阳对立”译作opposition of yin and yang，将“阴阳平衡”译作yin—yang balance等等。但也有一些有别于通行译法的翻译．如将“阴阳互根”译作mutual rooting of yin and yang，将“阴阳转化”译作yin-yang conversion等。这些译法基本上反映了某些西方译者的思路与方法。     

Quercetin suppresses HeLa cells by blocking PI3K/Akt pathway

To explore the effect of quercetin on the proliferation and apoptosis of HeLa cells, HeLa cells were incubated with quercetin at different concentrations. Cell viability was evaluated by MTT assay, cell apoptosis was detected by Annexin- V/PI double labeled cytometry and DNA ladder assay. Cell cycle was flow cytometrically determined and the morphological changes of the cells were ob- served under a fluorescence microscope after Hoechst 33258 staining and the apoptosis-related pro- teins in the HeLa cells were assessed by Western blotting. The results showed that quercetin signifi- cantly inhibited the growth of HeLa cells and induced obvious apoptosis in vitro in a time- and dose-dependent manner. Moreover, quercetin induced apoptosis of HeLa cells in cell cycle-dependent manner because quercetin could induce arrest of HeLa cells at G0/G1 phase. Quercetin treatment down-regulated the expression of the PI3K and p-Akt. In addition, quercetin could down-regulate ex- pression of bcl-2, up-regulate Bax, but exerted no effect on the overall expression of Akt. We are led to conclude that quercetin induces apoptosis via PI3k/Akt pathways, and quercetin has potential to be used as an anti-tumor agent against human cervix oancer.