甘肃省临夏回族自治州回族冠心病患者E-选择素基因多态性与冠心病相关性研究

目的 研究甘肃省临夏回族自治州回族E-选择素基因第2外显子G98T和第4外显子S128R多态性与冠心痛的相关性以及对血浆E-选择素水平的影响.方法 采用聚合酶链反应-限制性片断长度多态性技术检测甘肃省临夏回族自治州回族42例冠心病患者(冠心病组)及50例健康对照者(对照组)的E-选择素基因多态性,同时采用酶联免疫吸附法检测冠心病组和对照组血浆E-选择素水平.结果 冠心病组E-选择素基因S128R基因频率和等住基因频率高于对照组(P＜0.05).SR基因型携带者的血清E-选择素水平高于SS基因型.结论 甘肃省临夏回族自治州回族冠心病患者E-选择素S128R基因多态性与冠心病的发病有关,并可影响血浆E-选择素水平,R等位基因可能是冠心痛的候选基因,E-选择素基因G98T多态性在冠心病的发病中可能不起直接作用.     

E-选择素基因多态性与广西壮族人缺血性脑卒中的遗传易感性的关系

目的探讨E-选择素(E-selectin)基因多态性与广西地区壮族人缺血性脑卒中(ischemic stroke, IS)的关系.方法采用聚合酶链反应-限制性片段长度多态性(polymerase chain reaction-restriction fragment length polymorphism, PCR-RFLP)和DNA序列测定法检测205例IS及210 例对照组E-selectin基因第2外显子G98T、第4外显子A561C(S128R)多态性,同时采用酶联免疫吸附试验(enzyme linked immunosorbent assay, ELISA)检测IS和对照者血清E-selectin水平.结果 IS组E-selectin水平显著高于对照组(P<0.01),E-selectin基因S128R基因型频率和等位基因频率在IS组和对照组比较差异有显著性(P<0.05),基因型频率的相对风险分析发现,SR基因型携带者患IS的风险是SS基因型的2.008倍(OR=2.008,95%CI:1.144～3.524),SR基因型携带者的E-selectin血清水平显著高于SS基因型[(58.72±16.23) ng/ml vs (50.35±13.76) ng/ml, P<0.01].E-selectin 基因G98T基因型频率和等位基因频率在IS组和对照组之间比较差异无显著性(P>0.05).结论 E-selectin 基因S128R多态性与IS的发病具有相关性,R等位基因可能是广西地区壮族人IS发病的遗传易感基因,携带SR基因型的个体可能通过促进E-selectin的高度表达进而增加IS的发病风险.     

E-选择素S128R多态性与冠心病的关系

目的　探讨 E-选择素 S12 8R多态性与冠心病的关系。　方法　用聚合酶链反应 -限制性片段长度多态性 (PCR- RFL P)方法探查 E-选择素 S12 8R多态性在对照组和冠心病组中的基因频率分布。　结果　冠心病组 R等位基因频率高于对照组 (11.96 % vs 4 .71% ,P<0 .0 5 ) ,SR+RR基因型者空腹血浆 E-选择素水平高于 SS基因型者 (33.2 3± 7.2 0 vs 2 2 .5 8± 8.2 8ng/ m l,P<0 .0 5 )。　结论　 E-选择素 S12 8R多态性与冠心病的发生有关 ,R等位基因可能是冠心病的危险因素     

E-选择素S128R基因多态性与脑血栓形成遗传易感性的关系

目的 研究E-选择素(E-selectin)基因多态性与北方汉族人群脑血栓形成的关系.方法 应用聚合酶链反应限制性片段长度多态性(PCR-RFLP)技术检测120例脑血栓形成患者和102例健康体检者的E-选择素基因型;生化技术测定血脂、血糖水平;酶联免疫吸附试验(ELISA)检测血清E-选择素水平.结果 E-选择素S128R基因型在2组中的分布差异有显著性(X2=6.39,P<0.05),基因型频率的相对风险分析,SR基因型患脑血栓形成的风险是SS基因型的3.05倍,(OR=3.05,95%CI:1.29～7.20);等住基因频率在2组中差异也有显著性(X2=5.94,P<0.05,OR=2.84,95%CI:1.23～6.55).结论 E-selectin基因S128R多态性与脑血栓形成的发病具有相关性,R等位基因可能是北方汉族人脑血栓形成发病的遗传易感基因,携带SR基因型的个体可能通过促进E-selectin的高度表达进而增加脑血栓形成的发病风险.     

冠心病痰瘀证候与E选择素G98T及S128R基因多态性的关系研究

[目的]从基因多态性角度探讨冠心病痰瘀证的实质.[方法]入选107例研究对象(其中正常组20例,冠心病组87例),应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)核苷酸分型技术检测血清E选择素(E-selectin)G98T及S128R基因型,同时采用酶联免疫吸附法测定血清E-selectin浓度.[结果]冠心病痰瘀证组E-selectin G98T的GC基因型、G等位基因,E-selectin S128R的SR基因型、R等位基因频率高于正常组,但经过统计学分析,两者之间差异无显著性意义(P>0.05).冠心痛痰瘀证型组患者血清E-selectin水平高于正常组(P<0.01).[结论]冠心病痰瘀证可能与E选择素G98T的G等位基因、E选择素S128R的R等位基因有一定的相关性.     

E-选择素Leu554Phe基因多态性与冠心病发病的相关性研究

目的 研究辽宁西部地区汉族人群E-选择素基因Leu554Phe多态性,探讨该多态性与冠心病(CHD)发病的相关性.方法 应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术检测118例冠心病患者和125例正常对照者E-选择素基因型,生化技术测定血脂水平.结果 E-选择素基因型LL、LF、FF频率在病例组和对照组分别为84.75％,12.71％,2.54％和93.60％,6.40％,等位基因L、F频率在病例组和对照组分别为91.10％,8.90％ and 96.80％,3.20％.其基因型频率和等位基因频率在冠心病组和对照组比较均有显著性差异(P＜0.05).基因型频率的相对风险分析,LL+LF基因型患冠心病的风险是LL基因型的4.980倍(OR=2.633,95％CI:(1.098～6.312).结论 辽宁西部地区汉族人群E-选择素存在基因Leu554Phe多态性；E-选择素Leu554Phe基因多态性与冠心病的发病有相关性,F等位基因可能是冠心病发病的危险因素之一.     

E-选择素基因A561C(S128R)多态性与原发性高血压的关系研究

目的 探讨在中国人群中E-选择素基因A561C(S128R)多态性与原发性高血压的关系.方法 95例≤70岁的原发性高血压患者和101例年龄性别匹配的正常对照者,聚合酶链反应-限制性片段长度多态性方法检测E-选择素基因型,同时检测血脂、血糖、尿素、肌酐水平,ELISA测定血浆可溶性E-选择素水平.结果 我们发现3个基因型(AA,AC,CC),E-选择素基因型AA,AC,CC频率在原发性高血压组和对照组分别为78.9%、19%、2.1%和91.1%、7.9%、1.0%;等位基因A、C频率在原发性高血压组和对照组分别为88.4%、11.6%和95%、5%.其基因型频率和等位基因频率在原发性高血压组和对照组比较差异均有显著性(P＜0.05).基因型频率的相对风险分析表明AC基因型患原发性高血压的风险是AA基因型2.73倍(OR=2.73,95%CI=1.10～6.91).结论 E-选择素基因A561C(S128R)多态性明显影响血压,C等位基因可能是原发性高血压的危险因素之一.     

三磷酸腺苷结合盒转运子A1基因R219K多态性与血脂水平关系的研究

目的:探讨三磷酸腺苷结合盒转运子A1基因R219K多态性与血脂水平的关系,寻找冠心病的易感因素.方法:采用聚合酶链式反应—限制性片段长度多态性(PCR-RFLP)技术,对227例冠心病患者和162例非冠心病患者的ABCA1基因R219K位点G→A (Arg219Lys)进行检测,分析基因型频率和等位基因频率分布特点,以及与血脂水平的相关性.结果:与对照组比较,冠心病组RR基因型频率(42.3％)明显高于对照组(31.5％),差异有统计学意义(P＜0.05);而KK型频率(10.6％)显著低于对照组(20.7％),差异有统计学意义(P＜0.05).冠心病组中KK基因型携带者的三酰甘油(TG)水平(1.43±0.597) mmol/L低于RR基因型携带者(1.87±1.34) mmol/L(P＜0.05),KK型基因携带者高密度脂蛋白(HDL-C)水平为(1.34±0.35) mmol/L高于RR基因型携带者(1.09±0.18)mmol/L,差异有统计学意义(P＜0.05).结论:三磷酸腺苷结合盒转运子A1基因R219K多态性与血脂水平有关,是冠心病发病的易感因素之一.     

冠心病与三磷酸腺苷结合盒转运子A1基因R219K多态性关联的研究

目的探讨三磷酸腺苷结合盒转运子A1(ABCA1)基因R219K多态性与冠心病发病的相关性。方法采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术,对227例冠心病患者(冠心病组)和162例非冠心病患者(对照组)的ABCA1基因R219K位点G→A(Arg219Lys)进行检测,分析基因型和等位基因频率分布特点以及与血脂、冠心病发病的相关性。结果冠心病组RR基因型频率(42.3%)明显高于对照组(31.5%),差异有统计学意义(P<0.05);而KK型频率(10.6%)显著低于对照组(20.4%),差异有统计学意义(P<0.05)。突变纯合基因(KK)携带率在急性冠状动脉综合征(ACS)组为10.1%,稳定型心绞痛(SAP)组为11.6%,对照组为20.4%,其中ACS组与对照组间差异有统计学意义(P<0.05),而SAP组与ACS组间差异无统计学意义(P>0.05)。冠心病组中KK基因型携带者的三酰甘油(TG)水平为(1.43±0.59)mmol/L,低于RR基因型携带者的(1.87±1.34)mmol/L,差异有统计学意义(P<0.05),KK型基因携带者高密度脂蛋白胆固醇(HDL-C)水平为(...     

对氧磷酶基因1 Glnl92Arg多态性与青年缺血性脑卒中的关联研究

目的 探讨青年缺血性脑卒中与对氧磷酶基因1(PONI)Gln192Arg(Q192R)多态性的关系.方法 用聚合酶链反应技术检测2004年10月至2008年10月广西医科大学第一附属医院神经内科收治入院的131例青年缺血性脑卒中患者和135名健康对照者PONI基因Q192R多态性,并测定两组的血脂水平,同时应用颈动脉B超检测了131例患者颈动脉内膜中膜厚度.结果 青年缺血性脑卒中患者RR基因型分布显著高于对照组(P<0.05),RR基因型与青年缺血性脑卒中呈正相关[(OR=1.743,95%CI(1.032-2.943)].青年缺血性脑卒中患者和对照组192RR基因型携带者和非携带者比较,其血脂水平差异无统计学意义(P>0.05).青年缺血性脑卒中患者192RR基因型携带者和非携带者中吸烟、高血压、糖尿病的比例及体质指数差异无统计学意义;而携带者的颈动脉内膜中膜厚度较非携带者明显增加,差异有统计学意义(P<0.05).结论 PON1基因Q192R多态性与青年缺血性脑卒中的遗传易感性相关,其中RR基因型可能是青年缺血性脑卒中的危险因素.其机制有可能是通过影响颈动脉内膜中膜的厚度加速动脉粥样硬化而起作用.     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.