检测血清VEGF、CEA、TSGF在肿瘤中的临床意义

目的研究血清VEGF、CEA在肿瘤诊断中联合应用价值以及肿瘤患者中血清VEGF与TSGF相关性.方法收集结直肠癌、肺癌患者以及正常人静脉血标本,ELISA法检测血清VEGF含量,放射免疫法检测CEA,化学显色法检测TSGF.结果结直肠癌患者中,血清CEA阳性率为32.4%,VEGF阳性率为54.1%,两者联合应用时阳性率为73.0%.血清VEGF与TSCF在结直肠癌中的相关系数为r=0.355(P＜0.01),肺癌患者中为r=0.612(P＜0.05).结论联合检测CEA和VEGF可以明显提高结直肠癌患者的阳性率,肿瘤患者血清中VEGF与TSGF的含量显著相关.     

胃肠道肿瘤患者血清血管内皮生长因子、癌胚抗原检测的临床意义

目的:研究联合检测血清中血管内皮生长因子(VEGF)和癌胚抗原(CEA)在胃肠道肿瘤诊断中的应用价值。方法:收集胃癌、结直肠癌患者术前以及正常人血清标本,用ELISA法检测血清VEGF含量,用放射免疫法检测CEA含量。结果:胃癌、结直肠癌患者中,血清CEA阳性率分别为36.7%(11/30)、30.3%(10/33);VEGF阳性率分别为40.0%(12/30)、48.5%(16/33),联合检测时两者阳性率分别为60.0%(18/30)、75.8%(25/33)。结论:血清CEA和VEGF的联合检测可以明显提高胃癌和结肠癌患者的检出率。     

384例癌症患者HBV五项检测结果分析

报告 38 4例肝癌、胃癌、鼻咽癌患者乙肝病毒血清标志五项检测结果 ,阳性检出共 2 2 1例 ,阳性率 57.55% ,其中肝癌、胃癌、鼻咽癌的阳性率分别为 87.6%、60 .3 %、36.5%。肝癌、胃癌阳性率与健康人对照组比较差异有显著性 ( P <0 .0 1,P <0 .0 5) ,鼻咽癌阳性率与健康人对照组比较差异无显著性。提示 :肝癌、胃癌与HBV五项有密切关系 ,临床上应注意HBV五项阳性与肝癌、胃癌的临床表现相结合 ,进一步做好肝癌、胃癌的早期诊治工作     

结直肠癌患者手术前后血清CRP、CEA和 VEGF检测的临床意义

目的:探讨结直肠癌患者手术治疗前后血清CRP、CEA和VEGF水平的变化及临床意义。方法:对45例结直肠癌患者进行手术治疗前后血清CRP、CEA和VEGF水平检测,并与35名健康者作比较;分别计算比较15例术后复发患者CRP、CEA和VEGF阳性率和总阳性率。结果:结直肠癌患者治疗前血清CRP、CEA和VEGF水平显著高于健康对照组(P<0.05),术后2周其水平比术前有显著下降(P<0.05);而复发组15例,其水平有回升,与未复发组比较差异有统计学意义(P<0.05);术后复发患者三项指标检测的总阳性率为93%,明显高于单项检测阳性率最高的VEGF(P<0.05)。结论:检测结直肠癌患者血清CRP、CEA和VEGF水平的变化,对判断术后疗效和预测复发有重要的临床意义。     

血清miR-29c和miR-720表达在脑胶质瘤患者检测中的意义

目的观察血清miR-29c和miR-720表达在脑胶质瘤患者检测中的意义及其对预后的影响。方法选择术后病理证实为脑胶质瘤患者95例为脑胶质瘤组,35例健康体检者为对照组。用q RT-PCR的方法检测各组血清miR-29c和miR-720表达,观察脑胶质瘤组和健康对照组的miR-29c和miR-720表达水平变化,并观察脑胶质瘤患者血清miR-29c和miR-720表达水平与年龄,性别,肿瘤直径,病理类型,肿瘤分化程度,KPS评分和肿瘤部位的关系,手术前后miR-29c和miR-720表达水平的变化,及其高表达miR-29c和miR-720水平对1年生存率的影响。结果脑胶质瘤组患者血清miR-29c相对表达明显低于健康对照组(t=8.634,P0.01),手术后脑胶质瘤患者血清miR-29c表达量明显较治疗前升高(t=9.448,P0.01);而miR-720脑胶质瘤的相对表达量明显高于对照组(t=14.035,P0.01),手术后患者的miR-720表达水平明显较治疗前明显降低(t=13.462,P0.01)。脑胶质瘤患者血清miR-29c表达水平随着肿瘤分化程度升高而升高(P0.01),血清miR-720相对表达水平随着肿瘤分化程度升高而降低(P0.01)。血清miR-29c和miR-720相对表达水平与年龄,性别,肿瘤直径,病理类型,KPS评分和肿瘤部位无明显相关性(P0.05)。血清miR-29c高表达为42例,其1年生存率为95.24%(40/42),低表达组53例,其1年生存率为77.36%(41/53),高表达组明显高于低表达组(χ2=4.623,P0.05)。而血清miR-720高表达组51例,其1年生存率为76.47%(39/51),低表达组44例,其1年生存率为95.45%(42/44)例,高表达组明显低于低表达组(χ2=5.348,P0.05)。结论血清联合检测miR-29c和miR-720表达在脑胶质瘤诊断和预后判断具有重要价值,可作为一种诊断和病情评估的重要指标。     

LRG1在结直肠癌患者血清中的表达及临床意义

目的研究富含亮氨酸的α-2糖蛋白1(Leucine rich alpha-2 glycoprotein 1,LRG1)在结直肠癌患者血清的表达,探讨其临床意义。方法共纳入65例结直肠癌患者、55例结直肠良性肿瘤患者、50例健康志愿者;分别采用酶联免疫吸附法(Enzyme linked immunosorbent assay,ELISA法)检测血清LRG1水平,然后对三组患者血清中LRG1和CEA表达水平、结直肠癌血清LRG1与临床肿瘤分期的关系进行统计分析。结果与结直肠良性肿瘤组及健康查体组相比,结直肠癌组血清中LRG1和CEA水平明显偏高(P0.01),结直肠良性肿瘤组和正常对照组比较差异无统计学意义(P0.05);结直肠癌肿瘤分期与患者血清LRG1水平相关,Ⅲ、Ⅳ期恶性肿瘤患者血清LRG1水平明显高于Ⅰ、Ⅱ期恶性肿瘤患者,差异有统计学意义(P0.01);联合检测与单独检测LRG1相比,差异有统计学意义(Z=2.763,P0.01);与单独检测相比,联合检测准确率更高,差异有统计学意义(Z=3.034,P0.01);LRG1与CEA差异有统计学意义(Z=0.752,P0.05)。结论 LRG1可作为结直肠癌诊断潜在的生物学标记。     

EB病毒感染情况结合间接鼻咽镜筛查长沙市健康人群鼻咽癌

目的 检测EB病毒VCA-IgA在健康人群中的抗体水平,了解长沙市人群中的EB病毒感染情况,并结合间接鼻咽镜筛查鼻咽癌.方法 使用酶联免疫吸附法,检测EB病毒VCA-IgA水平.对于EB病毒VCA-IgA阳性个体,应用问接鼻咽镜检查鼻咽部,发现异常即取组织送病理检查.结果 长沙市1 808例健康人群EB病毒VCA-IgA检测:阳性者206例,检出阳性率为11.39%.其中男性阳性率为12.17%,女性阳性率为9.66%.男女无显著性差异(P=0.121).阳性个体EB病毒VCA-IgA抗体滴度在1:10~1:20之间.共对198例阳性个体进行间接鼻咽镜检查,最后病理学检查确诊鼻咽癌3例.结论 长沙市健康人群EB病毒感染率较高,存在相对较高的鼻咽癌患病风险;血清EB病毒VCA-IgA普查结合间接鼻咽镜是鼻咽癌人群筛查的有效手段.     

结直肠癌患者VEGF的检测及意义

目的 探讨结直肠癌患者血清VEGF含量检测及其临床意义.方法 检测复旦大学附属华山医院宝山分院普外二科及新疆医科大学肿瘤医院共64例已确诊的结直肠癌患者及48例健康体检者VEGF含量,比较结直肠癌患者在有无淋巴结、肝脏转移,Dukes不同分期时VEGF水平差异.结果 健康体检者VEGF水平低于结直肠癌患者(t=34.11,P＜0.05);有淋巴结转移的结直肠癌患者血清VEGF水平高于无淋巴结转移,有肝脏转移结直肠癌患者血清VEGF水平高于无肝脏转移的(均P＜0.05);TNMⅠ、Ⅱ、Ⅲ、Ⅳ期VEGF水平分别为(120.14±25.88)、(192.07±26.95)、(277.93±63.18)、(433.40±48.39) pg/ml,随着分期增加VEGF水平逐渐升高(F=83.11,P＜0.05).结论 VEGF的表达与结直肠癌的淋巴结转移、肝转移及TNM分期密切相关,有望成为判断预后的重要参考指标之一.     

抗survivin抗体与survivin mRNA在结直肠癌患者外周血中的表达及其临床意义

目的探讨结直肠癌(CRC)患者外周血中抗survivin抗体、survivin mRNA的表达情况及其临床意义。方法选择结直肠癌患者116例,依据所接受治疗手段,分为根治手术组、姑息手术组、姑息化疗组。对照组为30例健康体检者。分别于患者治疗前后抽取外周血,应用ELISA和半定量RT-PCR检测抗survivin抗体和survivin mRNA的表达水平。结果结直肠癌患者与健康者外周血中抗survivin抗体阳性率分别为40.5%和0,survivin mRNA阳性率分别为76.7%和0,差异均有统计学意义(P=0.000)。抗survivin抗体和survivin mRNA表达水平随肿瘤分期的增高而增高,肿瘤直径大于5cm者较直径小于或等于5cm者高(分别P=0.045、P=0.003),有淋巴结转移者较无淋巴结转移者显著增高(分别P=0.040、P=0.000);根治手术后较术前明显降低(分别P=0.005、P=0.004),根治术后表达水平高者转移或复发率显著高于表达水平低者(分别P=0.001、P=0.004);表达水平在姑息手术前后差异无统计学意义(分别P=0.709、P=0.669)。行姑息性化疗的患者中,有效组表达水平较化疗前明显降低(分别P=0.043、P=0.032);化疗无效组抗survivin抗体水平变化无统计学意义(P=0.135),而survivin mRNA表达较前显著升高(P=0.039)。结论外周血抗survivin抗体和survivin mRNA检测可能对CRC的诊断、预后估计、化疗疗效预测及随访有一定的临床意义。     

ConA识别的高甘露糖型糖蛋白在肿瘤患者血清中表达的研究

目的 研究ConA识别的高甘露糖型糖蛋白在鼻咽癌、肺癌、肝癌和结直肠癌患者血清中的表达规律.方法 应用ConA（刀豆凝集素）亲和层析法从正常人血清中获得ConA识别的高甘露糖型糖蛋白.结果 ConA识别的高甘露糖型糖蛋白明显高于正常人和良性疾病患者,阳性率分别为77.4%,65.2%,70.3%;鼻咽癌患者血清阳性率为21.4%.且在结直肠癌患者有效治疗后该糖蛋白水平显著下降,阳性率也下降.结论 血清中ConA识别的高甘露糖型糖蛋白,可用于肿瘤患者血清糖基化异常和肿瘤发病机理的研究,并可为临床诊断和治疗效果的评价提供参考指标.     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

Development on Adjustable Calibration Marker for Shock Wave Focus

Shock wave lithotripsy （SWL） is a treatment of choice for upper urinary stones. However, this procedure is inappropriate for obese patients because the focus is often unable to reach the target owing to the limited focal distance in shock wave source. Although treating such patients in a blast path may increase the application length of shock wave source, it＇s difficult to find this path on the lithotripter monitor. For this reason, we invented an adjustable calibration marker in order to set an effective focus in the shock wave hath.     

Study on the TCM Pathogenesis of Angiopathic Complications of Type II Diabetes

Excess production of reactive oxygen species(ROS)of mitochondrion mediated by hyperglycemia is the common pathogenesis of angiopathic complications of diabetes.TCM holds that the damp from the dysfunction of spleen.kidney and liver is the causative factor of complications of diabetes.This is similar to the mechanism of Ros resulting in angiopathic complications of diabetes.When the angiopathic complications of type II diabetes mellitus(T2DM)are difierentiated as caused by turbid damp in TCM can be explained as ROS.Since the obstruction of pathogenic damp in channels and collaterals is said to be the main pathogenesis,the treating principle should be dissolving the damp to remove the obstruction.     

Leptin, Ghrelin and TNF-α before and after Hypo-caloric Traditional Chinese Diet and Auricular Acupuncture

INTRODUCTION Obesity is a complex emergent problem, which can be possibly solved not only by the diet but also by the life style and promotion of a constant physical exercise. 1, 2 No doubt careful attentions must be given to the nutritional condition of obese people, the dietary habits, the somatic build (i.e. distribution of fat mass) and the organic functions linked to formation of the fat mass. All the parameters should be constantly monitored before, during and after a diet treatment. 3, 4, 5     

Relationship between Dysglycemia and Carotid Atherosclerosis in Tibetan Population

People with dysglycemia are at high risk for atherosclerotic diseases. This study aims at investigating the atherosclerotic vascular damage in dysglycemia and its metabolic origin in Tibetan population.     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

CLINICAL SIGNIFICANCE OF SERUM CYTOKINES IL-1β,sIL-2R,IL-6,TNF-α,AND IFN-v IN ACUTE CORONARY SYNDROME

Objectives To explore serum cytokines levels (including IL-1 β, sIL-2R, IL-6, TNF-α, and IFN-v) and their significance in patients with acute coronary syndrome (ACS) and the subsequent follow-ups, with attempt to estimate the role of various serum inflammatory markers in the diagnosis and assessment of ACS.Methods The study population include 40 patients with acute myocardial infarction (AMI), 40 patients with unstable angina pectoris (UAP), and 40 controls. Among the 80 patients, 60 patients attended a follow up 4 months later. Serum inflammatory markers including IL-1 β, sIL-2R, IL-6, TNF-α, and IFN-v were measured by enzyme linked immunosorbent assay.Results Serum IL- 1 β, sIL-2R, IL-6, TNF-α were significantly higher in AMI group or UAP group compared to the control group and became significantly lower 4 months later in the follow-up patients. Serum levels of IFN-v shows no significant difference between AMI group or UAP group and controls, also showing no significant change when measured in follow up patients. There was no correlation between serum creatine kinase-MB isoenzyme levels and serum inflammatory markers either in UAP or AMI group. Furthermore, when divided into two subgroups using Wagner's QRS scoring system in the AMI group, there is no difference of each serum inflammatory marker between ≤ 6 scores group and ＞ 6 scores group.Conclusion Serum levels of certain inflammatory markers may have some diagnostic value for ACS, and can be a useful marker reflecting disease stability.