六种冰冻混合血清酶学的短期同步稳定性研究

目的:观察冰冻混合血清六种酶(ALP、ALT、AST、GGT、CK、LDH)在室温(22～25℃)与冷藏(2～8℃)条件下的短期稳定性.方法:收集不同浓度的混合血清后过滤分装,采用同步设计稳定性研究方法,在Dimension RxL Max全自动生化分析仪上检测ALP、ALT、AST、GGT、CK、LDH六种酶的活性.采用双因素方差分析进行稳定性检验统计.结累:22～25℃贮存条件下,混合血清酶值在按照时间点分组时,ALP、GGT、AST、LDH水平(A)差异无统计学意义(P0.05);CK、ALT差异有统计学意义(P<0.05),LDH水平(8)随着贮存时间的延长,LDH活性有衰减的趋势.2～8℃贮存条件下,ALP、ALT差异有统计学意义(P<0.05);GGT、CK、AST差异无统计学意义(P0.05),LDH P=0.053.结论:同步设计稳定性方法是研究短期稳定性较好的方法;GGT、AST酶较稳定;ALT较不稳定;ALP酶在22～25℃贮存条件下较稳定;CK在2～8℃贮存条件下较稳定;LDH酶在22～25℃贮存条件下稳定性有待于进一步观察.     

混合血清中酶的短期同步稳定性研究

目的:观察混合血清中常见的六种酶(AST、ALT、CK、ALP、GGT、LDH)在冷冻(-20'C)、冷藏(2～89C)及室温(22～25℃)三种湿度条件下的短期稳定性.方法:收集混合体检血清高、中、低三个水平,过滤分装,每个水平分为63杯,其中11杯用于均匀性检测,52杯用于稳定性检测,各种酶的分析均在BECKMAN全自动生化仪Synchron LX20上完成.结果:在观察时间内,AST在三种温度条件下均有变化,在室温下变化最为显著;ALT在三种温度条件下均有明显变化,而且-20℃时变化反而大于2～8℃; CK室温储存变化显著,2～8℃条件下变化不明显,-20℃无变化;LDH在2～8℃与22～25℃下都有轻微变化,在-20℃无变化;ALP与GGT短期内均无变化.结论:不同的酶在不同的温度条件下稳定性不同,有些酶在-20℃保存仍不太稳定,如AST、ALT.实验室在对保存的标本进行复查时,应注意到酶的种类以及标本的保存条件,此外还要考虑标本是否已经被细菌污染.有些常温下贮存的标本,会有细菌生长,反而会使酶的水平升高.     

硫酸庆大霉素合剂的稳定性实验

目的 :预测硫酸庆大霉素合剂的贮存期。方法 :采用比色法 ,在 42 0nm波长处测定庆大霉素的含量 ,用恒温加速法预测其稳定性。结果 :在室温下 (2 5℃ )本品的有效贮存期为 6 .5 4个月。结论 :本制剂基本符合医院制剂的贮存要求。     

甲磺酸酚妥拉明片稳定性考察

目的　考察甲磺酸酚妥拉明片的稳定性。方法　甲磺酸酚妥拉明片在高温、高湿及光照条件下放置１０ ｄ ;４０ ℃,７５ ％ 湿度放置３ 个月;室温留样考察６ 个月,对其外观进行检查,对有关物质、溶出度和含量进行测定。结果　本品对光、热４０ ℃、高湿及空气稳定,对热６０ ℃和８０ ℃不稳定。结论　本品的稳定性较好,贮存时应密封置阴凉处,预计室温放置两年稳定。     

25%抗坏血酸注射液稳定性与稳定剂作用性质的关系

本文报告25％抗坏血酸注射液在贮存过程中(90℃加热实验模拟)颜色变化及稳定剂盐酸-L-半胱氨酸和焦亚硫酸钠的浓度变化。实验表明:注射液变色速度与稳定剂消耗量有关;后者稳定注射液颜色的能力优于前者,两者的稳定作用具独立性,稳定效果具加和性;加热实验的前1.6h,两种稳定剂共同产生稳定作用,1.6h后以焦亚硫酸钠的稳定作用为主;贮存过程中稳定剂的主要稳定作用不是与安瓿空间的氧作用。     

伊曲康唑胶囊稳定性考察

目的 考察伊曲康唑胶囊的稳定性。方法 伊曲康唑胶囊在高温、高湿及光照条件下放置１０ｄ;４０℃,７５℃湿度放置３个月。对其外观和内容物性状进行检查,对溶出度、有关物质及含量进行测定。结果 本品对光、热４０℃、湿９２．５％及空气稳定,对热６０℃、８０℃不稳定。结论 本品的稳定性较好,贮存时应密封置阴凉处,预计室温放置二年稳定。     

微球制剂条件下胸腺肽α1的稳定性评价

目的:研究微球制剂工艺条件下胸腺肽α1的稳定性.方法:采用高效液相色谱法(HPLC),研究胸腺肽α1在不同温度(-20℃、6～8℃、60℃)、不同超声时间(10～60 s)及不同 pH值(4.0、7.0、10.0)条件下的稳定性.结果:胸腺肽α1在-20℃冷冻保存7 d时仍很稳定,冷藏(6～8℃)时30 d内可保持稳定,60℃水浴中12 h内不降解;400 W超声60 s内不受影响; 37℃时置于pH 4.0的磷酸盐中12 h内比较稳定.结论:胸腺肽α1在本制备工艺条件下具有良好的稳定性.     

99mTc-PDGFR-β AODN的制备及其生物分布

目的:寻求一种理想的99mTc标记大鼠血小板衍化生长因子受体-β(PDGFR-β)反义脱氧寡核苷酸(AODN)的方法,并观察标记产物的生物学分布以探讨其用于防治冠状动脉再狭窄的可能性. 方法: 先使长度为18个碱基的单链PDGFR-β AODN与双功能螯合剂NHS-MAG3耦联,然后进行99mTc标记,测定不同条件下标记率,分析确定最佳标记条件.常规测定放化纯度和比活度,并对标记物进行稳定性和正常小鼠体内分布实验. 结果: ①最佳标记条件下平均标记率达70%(最高为73.2%),经P4层析柱纯化后放化纯度>95%,比活度7.4～11.1MBq(200～300μCi)/ μg;② 99mTc-MAG3-AODN在室温下生理盐水中及37℃下新鲜人血清中稳定性良好,与血清蛋白结合率为6%～8%;③ 99mTc-MAG3-AODN在正常小鼠体内稳定性良好,在肾、肝中摄取较高. 结论: 以NHS-MAG3为鳌合剂标记得到的99mTc-MAG3-AODN具有良好的稳定性,为下一步进行细胞和动物实验提供了基础.     

头孢曲松钠输液稳定性影响因素研究

目的 :了解影响头孢曲松钠 (CS)在静脉输液中稳定性的因素 ,以提高药效。方法 :观察CS在不同液体、保存时间、温度下的药物稳定性。结果 :CS在 2 0℃时 ,在 0 9%氯化钠注射液中稳定性最好 ;常用输液液体中 ,5℃条件下 ,2 4h内稳定性 >97% ,5～35℃条件下 ,6h内稳定性也 >90 %。结论 :室温 (2 0℃ )下 ,CS应即配即滴 ,并在 6h内滴完 ,输液配伍以选择 0 9%氯化钠注射液为最佳     

替硝唑葡萄糖注射液中替硝唑的稳定性预测

目的 :预测医院制剂替硝唑葡萄糖注射液的稳定性 ,为临床提供贮存参考。方法 :采用经典恒温加速法 ,分别于 65℃、 70℃、75℃、80℃恒温条件下 ,在不同间隔时间内用紫外分光光度法测定替硝唑的含量。结果 :其稳定性与温度相关 ,符合Arrhenius公式。结论 :该制剂在室温 2 5℃ ,避光条件下贮存期为 2 3 4Y。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

Acupuncture Combined with Spinal Tui Na for Treatment of Primary Dysmenorrhea in 30 Cases

Objective: To observe the therapeutic effects in acupunture treatment of primary dysmenorrhea combined with spinal Tui Na, and study its mechanism. Methods: Thirty cases of the treatment group were treated by acupuncture combined with spinal Tui Na, and thirty cases in the control group were treated by routine acupuncture. Results: The total effective rate was 93.3% in the treatment group, and 73.3% in the control group, with a significant difference between the two groups (P<0.05). Conclusions: Acupuncture combined with spinal Tui Na has good prospects for treatment of primary dysmenorrhea.     

Luo Lingjie's Experience in Treating Chronic Nephropathy

In treating chronic nephropathy,Luo Lingjie,a chief physician,pays attention to regulating the balance between yin and yang,treating infection if present,and removing pathogenic factors.He prescribes gentle drugs and uses carefully strongly warming-tonifying ones,emphasizes the importance of persuading the patient to persist in treatment with medication and nurse one's health for recuperation,and is good at combined use of TCM and western medicine therapy and brings the merits of various therapies into full play,with obvious theraoeutic effects.     

Dr.Zhang Ren's Experience in the Acupuncture Treatment of Different Diseases with the Same Therapeutic Principle

Dr.Zhang Ren,the chief physician,is the chairman of Shanghai Acupuncture and Moxibustion Association.Having been engaged in medicine for about 40 years,he is experienced in treating various intractable diseases.In his long years of clinical practice,he advocates taking the TCM differentiation as the basis to seek for the acupuncture method for treatment of modern intractable diseases.The author of this essay had the fortune to follow Dr.Zhang in study.The following is a summary of Dr.Zhang's experience in the acupuncture treatment for different intractable diseases with the same therapeutic principle.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.