哮喘患儿免疫球蛋白E和白细胞介素-17检测的意义

引言 支气管哮喘（简称哮喘）是儿童常见的慢性呼吸道疾病之一,是一种以气道高反应性和气道慢性炎症为特征的变态反应性疾病,它是由多种炎性细胞和细胞因子参与的炎症过程．我们检测30例哮喘患儿急性发作期血清白细胞介素．17（IL-17）和免疫球蛋白E（IgE）水平变化,并作相关性分析,探讨IL-17及Ig—E在哮喘发病机制中的作用．     

小儿支气管哮喘的发病因素及预防

支气管哮喘（简称哮喘）是一种全球控的呼吸道变态反应性疾病，严重影响小儿身心健康。其本质是由多种细胞特别是嗜酸细胞和肥大细胞、嗜碱细胞、浆细胞等参与的气道慢性炎症（AAI）致气造高反应性（AHR）。神经源性炎症参与AAI过程，与哮喘发病有关D’。1发病因素哮喘的形成与发作有许多复杂的综合因素。目前认为，本病是一种与多因素相关的遗传性疾病，由遗传与环境因素协同作用形成。发病因素有：互．l遗传因素“特定生”是哮喘形成最易确认的危险因素，是由遗传所决定的对普通环境过敏原能过度产生IgE的一种倾向。特应性家庭小儿患…     

支气管炎哮喘患儿血25-（OH）-D3、尿LTE4的临床研究

目的探讨、验证维生素D是否参与支气管哮喘的发病及探寻支气管哮喘病情监测和辅助治疗新方法。方法收集2011年12月—2014年1月就诊于包头医学院第一附属医院的支气管哮喘患儿（60例）、喘息性支气管肺炎患儿（44例）、门诊健康体检儿童（30例）,分别取血及尿液,进行血25-（OH）-D3、总Ig E、尿LTE4含量的测定。结果血25-（OH）-D3水平支气管哮喘组较喘息性支气管肺炎组、健康对照组低,差异具有统计学意义（P〈0.05）;尿白三烯E4水平支气管哮喘组较喘息性支气管肺炎组、健康对照组高,喘息性支气管肺炎组较健康对照组高,差异均具有统计学意义（P〈0.05）;总Ig E水平支气管哮喘组较喘息性支气管肺炎组、健康对照组高,差异均具有统计学意义（P〈0.05）;喘息性支气管肺炎组较健康对照组血总Ig E高,差异有统计学意义（P〈0.05）;哮喘组血总Ig E、尿LTE4呈正相关,血25-（OH）-D3与血总Ig E、尿LTE4均无相关。结论 25-（OH）-D3参与了哮喘的发病,尿LTE4可做为监测儿童哮喘病情的又一指标。     

病毒性呼吸道感染与支气管哮喘

病毒性呼吸道感染与支气管哮喘孙凤春综述胡华震审校（山东苍山县医院哮喘研究室２７７７００）（附属弋矶山医院肺科）２０年前，临床上已注意到呼吸道急性感染与支气管哮喘（简称哮喘）密切相关。其后的大量流行病学调查和研究均表明，病症性呼吸道感染（Ｖｉｒａｌｒｅ...     

肺泡巨噬细胞与支气管哮喘的研究进展

支气管哮喘是由多种炎性细胞参与的呼吸道慢性炎症。有关嗜酸性粒细胞（EOS）、肥大细胞、嗜碱性粒细胞（EC）、淋巴细胞及其细胞组分在哮喘发病中的作用已有了大量研究，对哮喘的发病机制做了颇多有益探索。但对肺泡巨噬细胞（AM）在哮喘发病中的作用研究较少，现将近年对AM的研究进展综述如下。     

肺炎支原体感染与支气管哮喘相关性研究

目的探讨肺炎支原体感染与支气管哮喘的相关性。方法检测84例哮喘组和66例对照组下呼吸道感染患者的外周血肺炎支原体抗体（MP—IgM）、血清总免疫球蛋白E（IgE）及外周血嗜酸性粒细胞（EOS）;对比两组MP—IgM阳性率,对AMP培M阳性与阴性患者的幢E和EOS。结果哮喘组MP—IgM阳性率（53.57％）显著高于对照组（33.34％）．差异有统计学意义（“P〈0.05”）;MP—IgM阳性患者的IgE和EOS显著高于MP—IgM阴性患者,差异有统计学意义（“p〈0.05”）。结论肺炎支原体感染与支气管哮喘在发病机制方面存在密切的相关性。     

支气管哮喘病人的心理特征及护理

支气管哮喘病人的心理特征及护理宋力，王金红，欧玉秋支气管哮喘是常见的心身疾病之一，其发病不单纯是下呼吸道炎性介质的产生、释放及炎性细胞的浸润，而且涉及到病人的心理活动。病人的情绪和个性因素的变化对哮喘发病有着直接的影响。所以，对支气管哮喘病人的治疗除...     

CHL1在支气管哮喘中的表达及意义

目的：探讨神经细胞黏附分子L1样蛋白（cell adhesion molecule L1?like protein，CHL1）在支气管哮喘中的表达及潜在机制。方法：收集55例哮喘患者及同期18例健康对照的临床资料及血清标本，酶联免疫吸附法检测血清CHL1水平，分析其与肺功能指标、血清总免疫球蛋白E（immunoglobulin E，IgE）的相关性。构建卵清蛋白（ovalbumin，OVA）诱导的慢性过敏性哮喘小鼠模型，免疫组化观察小鼠肺组织CHL1的表达定位及分布。采用不同细胞因子刺激人支气管上皮细胞（16HBE），分别用实时定量PCR法和蛋白质免疫印迹法检测细胞CHL1 mRNA和蛋白表达情况。结果：与对照组（4.174±2.122）ng/mL相比，哮喘患者血清CHL1水平明显增高［（7.497±3.274）ng/mL，P < 0.000 1］，与血清总IgE水平呈正相关（r=0.287，P=0.048）。CHL1主要表达于小鼠肺组织支气管上皮细胞，哮喘小鼠表达量较对照组升高。TGF?β可上调支气管上皮细胞CHL1 mRNA及蛋白表达水平。结论：哮喘中支气管上皮细胞CHL1表达增加，该过程可能与TGF?β相关信号通路有关。     

支气管哮喘患者五种细胞因子水平测定及其临床意义

支气管哮喘是一种严重危害人群健康的疾病，由于其病情迁延、反复发作，严重影响了人类的身心健康。支气管哮喘（以下简称哮喘）是由多种细胞（如嗜酸性细胞、肥大细胞、T淋巴细胞、中性粒细胞、气道上皮细胞等）和细胞组分（cellular elements）参与的气道慢性炎症性疾患。白介素（IL）和肿瘤坏死因子（TNF—α）在这种炎症反应过程中对增强、维持和减轻炎症反应起了重要的调节作用。我们2003年10月-2005年12月选择来自我院呼吸内科门诊或住院的3、4级支气管哮喘患者65例，在治疗前后行5种细胞因子水平检测，结果报告如下。     

支气管哮喘的诊治

支气管哮喘（简称哮喘）是一种严重危害人类健康的慢性呼吸道疾病,目前全球约3亿哮喘患者,每年有25万人死于哮喘。哮喘是多种细胞包括气道的炎症细胞和结构细胞（如嗜酸粒细胞、肥大细胞、淋巴细胞、中性粒细胞和气道上皮细胞致气道高反应性,并引起反复发作性的喘息、气急、胸闷或咳嗽等症状。     

Up-regulated expression of Tim-3/Gal-9 at maternal-fetal interface in pregnant woman with recurrent spontaneous abortion

The relationship between T cell immunoglobulin domain and mucin domain protein 3（Tim-3）/Galectin（Gal）-9 pathway and recurrent spontaneous abortion（RSA） was studied. Thirty-one pregnant women with RSA and 27 normal early gravidas were investigated to detect the levels of Tim-3 and Gal-9 in villi and deciduas by Western blotting. Meanwhile, the concentration of interleukin（IL）-4 and IL-12 in peripheral blood plasma was determined by ELISA in 25 healthy fertile non-pregnant controls, the normal early gravidas and pregnant women with RSA mentioned above, respectively. It was found that the relative expression levels of Tim-3 and Gal-9 in villi and deciduas were significantly increased in pregnant women with RSA as compared with those in the normal early gravidas. The concentration of IL-4 in peripheral blood plasma of pregnant women with RSA was lower than that of the normal early gravidas（P〈0.05） and healthy fertile non-pregnant controls（P〈0.05）, but that of IL-2 in pregnant women with RSA was significantly higher than that of the normal early gravidas（P〈0.05） and healthy fertile non-pregnant controls（P〈0.05）. It was suggested that the overexpression of Tim-3/Gal-9 pathway may be related to the pathogenesis of RSA.     

Comparative study of mutation spectrums of MT-RNR1 m.1555A>G, GJB2, and SLC26A4 between familial and sporadic patients with nonsyndromic sensorineural hearing loss in Chinese Han

Background The mutation frequencies of three common deafness genes （MT-RNR1 m.1555A〉G,GJB2,and SLC26A4） among patients with nonsyndromic sensorineural hearing loss （NSHL） were different in previous studies.Inconsistent selection criteria for recruiting patients could have led to differences in estimating the frequencies of genetic mutations thus resulting in different mutation frequencies among these studies.The aim of this study was to reveal the differences in the mutation spectrums of the three common genes between familial and sporadic Chinese Han patients.Methods Totally,301 familial probands and 703 sporadic patients with NSHL were enrolled in this study.Three genes,MT-RNR1 m.1555A〉G,GJB2,and SLC26A4,were screened for mutation in our study cohort.A X2 test was performed to compare the mutation frequencies between the two groups.Results The study showed that the disease-causing mutation frequencies of MT-RNR1 m.1555A〉G,GJB2,and SLC26A4 were 12.29%,14.62%,and 18.27% in familial probands and 3.56%,18.63%,and 18.92% in sporadic patients,respectively.The mutation frequency of MT-RNR1 m.1555A〉G in familial probands was significantly higher than in sporadic patients （X2 test,P=0.000）,while there were no significant differences in the mutation frequencies of GJB2 and SLC26A4 between the familial and sporadic groups （X2 test,P 〉0.05）.Conclusions It is necessary to reveal the differences in gene mutation frequencies between patients of different sources or characteristics by comparative studies in order to avoid selection bias.The mutations of GJB2,SLC26A4,and MTRNR1 m.1555A〉G are the most important etiological factors in Chinese Han patients,among which SLC26A4 might be the most frequent.     

Sphingosine Kinase-1/sphingosine 1-phosphate pathway in diabetic nephropathy

Objective Diabetic nephropathy （DN） is the major cause of end-stage renal disease worldwide and its prevalence continues to increase.Currently,therapies for DN provide only partial renoprotection; hence new targets for therapeutic intervention need to be identified.In this review,we summarized the new target,sphingosine kinase-1/sphingosine 1-phosphate （SphK1/S1P） pathway,explored its potential therapeutic role in the prevention and treatment of DN.Data sources Most relevant articles were mainly identified by searching PubMed in English.Study selection Mainly original articles and critical review articles by major pioneer investigators in this field were selected to be reviewed.Results SphK1/S1P pathway can be activated by hyperglycemia,advanced glycation end products,and many proinflammatory cytokines,which leads to fibronectin,transforming growth factor-31 up-regulation and AP-1 activation.And then it could promote glomerular mesangial cells proliferation and extracellular matrix accumulation,mediating the initiation and progression of diabetic renal fibrosis.Conclusions SphK1/S1P pathway is closely correlated with the pathogenesis of DN.The results suggest that SphK1/ S1P pathway as a new target for clinically improving DN in future is of great prospect.     

MiR-503 regulates cisplatin resistance of human gastric cancer cell lines by targeting IGF1R and BCL2

Background Studies have shown that the drug resistance of gastric cancer cells can be modulated by abnormal expression of microRNAs （miRNAs）.We investigated the role of miR-503 in the development of cisplatin resistance in human gastric cancer cell lines.Methods MiR-503 expression was measured by quantitative real-time PCR.MTT （3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bromide） and clonogenic assays were used to examine changes in cell viability and the drug resistance phenotype of cancer cells associated with upregulation or downregulation of the miRNA.A dual-luciferase activity assay was used to verify target genes of miR-503.Immunohistochemistry,Western blotting analysis,and a flow cytometric apoptosis assay were used to elucidate the mechanism by which miR-503 modulates drug resistance in cancer cells.Results MiR-503 was significantly downregulated in gastric cancer tissues and several gastric cancer cell lines.Additionally,downregulation of miR-503 in the cisplatin （DDP）-resistant gastric cancer cell line SGC7901/DDP was concurrent with the upregulation of insulin-like growth factor-1 receptor （IGF1R） and B-cell lymphoma 2 （BCL2） expression compared with the parental SGC7901 cell line.An in vitro drug sensitivity assay showed that overexpression of miR-503 sensitized SGC7901/DDP cells to cisplatin.The luciferase activity of reporters driven by IGF1R and BCL2 3＇-untranslated regions in SGC7901/DDP cells suggested that IGF1R and BCL2 were both direct target genes of miR-503.Enforced miR-503 expression in SGC7901/DDP cells reduced expression of the target proteins,inhibited proliferation,and sensitized the cells to DDP-induced apoptosis.Conclusion Our findings suggest that hsa-miR-503 modulates cisplatin resistance of human gastric cancer cells at least in part by targeting IGF1R and BCL2.     

Effects of IL-17 on expression of GRO-α and IL-8 in fibroblasts from nasal polyps

Recent studies indicated that interleukin（IL）-17, growth-related oncogene（GRO）-α and IL-8 play an important role in the pathogenesis of nasal polyps. However, the effects of the increased amount of IL-17 and the production of GRO-α and IL-8 in human nasal polyp fibroblasts are not completely understood. This study aimed to determine the effects of the increased IL-17 on the changes of GRO-α and IL-8 expression in human nasal polyp fibroblasts and further investigate the mechanism of neutrophil infiltration in nasal polyps. Nasal polyp fibroblasts were isolated from six cases of human nasal polyps, and the cells were stimulated with five different concentrations of IL-17. Real-time fluorescence quantitative polymerase chain reaction（RT-PCR） was used to detect the mRNA expression of GRO-α and IL-8. The mRNA of GRO-α and IL-8 was expressed in unstimulated controls and remarkably increased by stimulation with IL-17. Moreover, the levels of GRO-α and IL-8 produced by fibroblasts were increased gradually with the increases in IL-17 concentrations. The present study showed that nasal fibroblasts can produce GRO-α and IL-8, and their production is remarkably enhanced by IL-17 stimulation, thereby clarifying the mechanism of the IL-17 mediated neutrophil infiltration in nasal polyps. These findings might provide a rationale for using IL-17 inhibitors as a treatment for nasal inflammatory diseases such as nasal polyps.     

Effect of behavior training on learning,memory and the expression of NR2B,GluR1 in hippocampus of rats offspring with fetal growth restriction

Objective： To study effects of behavior training on learning, memory and the expression of NR2B, GluR1 in hippocampus of rat＇ s offspring with fetal growth restriction（FGR）. Methods： The rat model of FGR was established by passive smoking method. The rats offspring were divided into the FGR group and the control group, then randomly divided into the trained and untrained group, respectively. Morris water maze test was proceeded on postnatal month（PM2/4） as a behavior training method, then the learning-memory of rats was detected through dark-avoidance and step-down tests. The expressions of NR2B and GluR1 subunits in hippocampal CA1 and CA3 areas were detected by immunohistochemical method. Results： In the dark-avoidance and step-down tests, the performance record of rats with FGR was worse than that of control rats, and the behavior-trained rats was better than the untrained rats, when the FGR model and training factors were analyzed singly. The model factor and training factor had significant interaction（P 〈 0.05）. The expressions of NR2B and GluR1 subunits in hippocampal CA1 and CA3 areas of rats with FGR reduced. In contrast, the expressions of GluR1 and NR2B subunits in CA1 area of behavior-trained rats increased, when the FGR model and training factors were analyzed singly. Conclusion： These findings suggested that the effect of behavior training on the expressions of NR2B and GluR1 subunits in CA1 area should be the mechanistic basis for the training-induced improvement in learning-memory abilities.     

Comparison of Fresh Blood Cell Nature Immune Reaction of Children Patients with SLE or RA

To research the erythrocytes of condition of innate immunity in the children patients with RA and SLE, comparing with changes of activity of CD35 （CR1） on the erythrocyte between these two kinds of diseases, and further discuss the pathogenesis of these two kinds of diseases in children. The reaction of innate immunity of erythrocytes, lymphocytes and neutrophils which isolated from fresh blood of patients with RA and SLE to tumor cells was measured, and then the levels of expression of CD35 by using flow cytometry （FCM） were measured. The rosette rate of erythrocyte, lymphocytes and granulocytes adhering to cancer cell in the children patients with RA were higher than counterparts of normal controls （P〈0.001） respectively; the rosette rate of erythrocyte, lymphocytes and granulocytes adhering to tumor cells in the children patients with SLE were lower than counterparts of normal controls （P〈0.001） respectively. The rosette rate of these to tumor cells of children patients with RA, showed the highest figure change during different phases, including progression phase〉stationary phase 〉 extinction phase. And the rosette rates in these three groups were significantly higher than ones of that in normal controls. The expression of CD35 on the erythrocyte of children patients with RA was significantly higher than that of normal controls （P〈0.001）, while that of patients with SLE was significantly lower than that of normal controls （P〈0.001）. The CD35 on the red cells showed significantly positive correlation （γ=0.804, P〈0.01） with adhering erythrocytes and positively correlation with the rate of lymphocytes （P〈0.05）. The native immune function of children patients with SLE or RA was out of balance, and perhaps its changes have a relation with activity of diseases. It was suggested that the erythrocyte immunity function may be a new indicator to the condition of children patients with RA or SLE to judge the development and prognosis.