Effects of 3，4－Dihydroxyacetophenone on Hypoxic Vasoconstriction in Isolated Pulmonary and Basilar Arterial Rings

Ｅｆｆｅｃｔｓｏｆ３，４－ＤｉｈｙｄｒｏｘｙａｃｅｔｏｐｈｅｎｏｎｅｏｎＨｙｐｏｘｉｃＶａｓｏｃｏｎｓｔｒｉｃｔｉｏｎｉｎＩｓｏｌａｔｅｄＰｕｌｍｏｎａｒｙａｎｄＢａｓｉｌａｒＡｒｔｅｒｉａｌＲｉｎｇｓＦａｒｍａｎＵｌｌａｈ，ＷａｎｇＤｉ－ｘｕｎ（王...     

Experimental Studyon Anti－tumor Effect of SplenocytesInduced by Anti－CD3McAb，PHA and IL－2

（沈张悦）（邵静芳）（王晓林）（朱慧芬）ＥｘｐｅｒｉｍｅｎｔａｌＳｔｕｄｙｏｎＡｎｔｉ－ｔｕｍｏｒＥｆｆｅｃｔｏｆＳｐｌｅｎｏｃｙｔｅｓＩｎｄｕｃｅｄｂｙＡｎｔｉ－ＣＤ３ＭｃＡｂ，ＰＨＡａｎｄＩＬ－２￥ＳＨＥＮＧｕａｎ－ｘｉｎ，ＷＡＮＧＸｉａｏ－ｌｉ...     

The Screening of Colorectal Cancerby Enzymatic Detection of Sialic Acid－free β－D－Gal（1→3）－D－GalNAc in Rectal Mucus

ＴｈｅＳｃｒｅｅｎｉｎｇｏｆＣｏｌｏｒｅｃｔａｌＣａｎｃｅｒｂｙＥｎｚｙｍａｔｉｃＤｅｔｅｃｔｉｏｎｏｆＳｉａｌｉｃＡｃｉｄ－ｆｒｅｅβ－Ｄ－Ｇａｌ（１→３）－Ｄ－ＧａｌＮＡｃｉｎＲｅｃｔａｌＭｕｃｕｓＭｕＣｈｕａｎｊｉｅ（穆传杰）；ＬｉＳｈｉｓｈｅ...     

7－N，N－二甲基－1，2，3，4，5－五硫环辛烷经巴比妥预处理大鼠肝微粒体体外代谢的研究

７－Ｎ，Ｎ－二甲基－１，２，３，４，５－五硫环辛烷经巴比妥预处理大鼠肝微粒体体外代谢的研究［ＸｉａＳｈｉｊｕｎ（夏世钧），ＺｈａｏＳｈｕｓｈｅｎ（赵书申），ＺｈｏｕＰｉｑｕａｎ（周丕泉）等。ＰｅｓｔｉｃｉｄｅＢｉｏｃｈｅｍｉｓｔｒｙａｎｄＰｈｙｓｉｏ...     

3H－1，2－二氢－1－吡咯里嗪酮衍生物的合成

实验证实３Ｈ－１，２二氢－１－吡咯里嗪酮（Ａ）及５－（４－氯苯甲酰基）３Ｈ－１，２－二氢－１－吡咯里嗪酮（Ｂ）具有明显的抗炎镇痛作用。在此基础上，设计合成了Ａ的１２个Ｍａｎｎｉｃｈ碱衍生物Ｃ（１－１２）及Ｂ的缩氨脲Ｄ１和肟Ｄ２，这１４个化合物均未见文献报道。药理实验证明，这些化合物大多具有抗炎镇痛活性，其中化合物Ｃ１、Ｃ２、Ｃ３、Ｃ９活性最为显著。     

Effects of Dopamine on the Maximal Mouth Respiratory Pressure－A Random，Double－blind，Placebo－controlled，Crossover Study

ＥｆｆｅｃｔｓｏｆＤｏｐａｍｉｎｅｏｎｔｈｅＭａｘｉｍａｌＭｏｕｔｈＲｅｓｐｉｒａｔｏｒｙＰｒｅｓｓｕｒｅ─ＡＲａｎｄｏｍ，Ｄｏｕｂｌｅ－ｂｌｉｎｄ，Ｐｌａｃｅｂｏ－ｃｏｎｔｒｏｌｌｅｄ，ＣｒｏｓｓｏｖｅｒＳｔｕｄｙＺｈａｏＹｉｊｕ（赵一举），Ｃａｉ...     

中国南京地区汉族人HLA－DR4DNA的快速分型

应用ＡＲＭＳ（ａｍｐｌｉｆｉｃａｔｉｏｎｒｅｆｒａｃｔｏｒｙｍｕｔａｔｉｏｎｓｙｓｔｅｍ）方法对南京地区１００例汉族健康人ＨＬＡ－ＤＲ４进行基因分型，结果表明，本地区汉族人ＨＬＡ－ＤＲ４基因纯合子占６％，杂合子占１５％，非ＤＲ４占７９％。本法较ＰＣＲ／ＳＳＯ法简单、快速，结果可靠，可用于ＨＬＡ配型。检测结果为ＨＬＡ相关联疾病及人类学的研究提供了参考依据     

Preparation and Characterization for Bispecific Antibodies of Anti-CD3×Anti-Idiotype to BCellLym phocytic Leukem ia

Ａｎｔｉ－ＣＤ３×ａｎｔｉ－ｉｄｉｏｔｙｐｅ（Ｉｄ）ｂｉｓｐｅｃｉｆ－ｉｃａｎｔｉｂｏｄｙ（ＢｓＡｂ）ｃａｎｓｉｍｕｌｔａｎｅｏｕｓｌｙｒｅｃ－ｏｇｎｉｚｅＣＤ３ｍｏｌｅｃｕｌｅｏｎＴｃｅｌｌｓａｎｄＩｄａｎｔｉ－ｇｅｎｉｃｄｅｔｅｒｍｉｎａｎｔｏｎＢｃｅｌｌｍｅｍｂｒａｎｅｏｆＢｃｅｌｌｌｙｍｐｈｏｃｙｔｉｃｌｅｕｋｅｍｉａ（Ｂ－ＣＬＬ）．ＴｈｅＢｓＡｂｃａｎｂｏｔｈａｃｔｉｖａｔｅＴｃｅｌｌａｎｄｅｎｈａｎｃｅｉｔｓｃｙｔｏｔｏｘｉｃａｃｔｉｖｉｔｙ，ａｎｄｓｐｅｃｉｆｉｃａｌｌｙｌｅａｄａ…     

Costimulation signals of LFA－1，LFA－2，CD44 and CD45 molecules in T cell activation

ＣｏｓｔｉｍｕｌａｔｉｏｎｓｉｇｎａｌｓｏｆＬＦＡ－１，ＬＦＡ－２，ＣＤ４４ａｎｄＣＤ４５ｍｏｌｅｃｕｌｅｓｉｎＴｃｅｌｌａｃｔｉｖａｔｉｏｎＳｈｅｎＦｅｎｇ；（沈锋）；ＷｕＭｅｎｇｃｈａｏ；（吴孟超）；ＧｕｏＹａｊｕｎ；（郭亚军）；ＣｈｅｎＨａｎ；...     

Amplification of three oncogenes c－myc，N－ras and c－erbB of ovarian tumor tissue

Ａｍｐｌｉｆｉｃａｔｉｏｎｏｆｔｈｒｅｅｏｎｃｏｇｅｎｅｓｃ－ｍｙｃ，Ｎ－ｒａｓａｎｄｃ－ｅｒｂＢｏｆｏｖａｒｉａｎｔｕｍｏｒｔｉｓｓｕｅ￥ＸｉｎＸｉａｏｙａｎ（辛晓燕）；ＬｉＧｕｉｙｏｎｇ（李贵容）；ＺｈｅｎｇＷｅｉｇｕｏ（郑维国）（Ｄｅｐａｒｔｍ...     

Sympathetic innervation of pulmonary circulation and its role in hypoxic pulmonary vasoconstriction

The purposes of this study were to localize the position of spinal center of sympathetic nerve which controls the pulmonary circulation, and to evaluate the role of pulmonary sympathetic nerve in hypoxic pulmonary vasoconstriction (HPV) in pithed rat model. The sympathetic preganglionic fibers arising from C₇–T₁₀ segments were stimulated electrically in succession. During stimulation the pulmonary vascular resistance (PVR) was increased in all segments tested, most significantly in C₇-T₄ (about 28 % above control value), obviously higher than that in T₅-T₁₀. The higher the stimulated spinal segments, the larger the ratio of ΔPVR/ΔSVR. Alveolar hypoxia (12%O₂–88% N₂) could induce HPV in pithed rat. In the presence of hypoxia, stimulation of T₁–T₃ segments caused a double increment in PVR and -P_p, as compared with those during normoxia (P<0.05). The data show that 1) the spinal center of sympathetic nerve regulating the vasomotion of pulmonary circulation is located in the C₇-T₄ segments; 2) the excitation of sympathetic nervous system during hypoxia could enhance HPV.     

急性等容血液稀释时体循环和肺循环变化的实验研究

目的 :研究急性等容血液稀释 (ANHD)对体循环及肺循环的影响 ,指导临床ANHD的应用。方法 :10只健康成年杂种犬 ,麻醉后气管插管 ,控制呼吸 ,正常体温下 ,每只犬行三个水平的ANHD ,即中度 (HD1Hct约为 19% )、深度 (HD2 Hct约为 15 % )、极深度 (HD3 Hct约为 9% ) ,然后回输自家血 ,在血液稀释前、稀释后 30min、回输血后 15min分别测血液流变学、体循环和肺循环各指标。结果 :HD时血粘度 η渐下降。HD1、HD2 时 ,心输出量 (CO)和心脏指数 (CI)分别升高 41%和 48% ;心率 (HR)无明显变化 ,总外周阻力 (SVR)渐下降 ,平均动脉压 (MAP)渐下降 ;肺小动脉阻力 (PVR)渐下降 ,而平均肺动脉压力 (MPA)、肺动脉楔入压 (PCWP)、中心静脉压 (CVP)无明显变化。HD3 时 ,CO(CI)不再升高反而降低 ,HR仍无明显变化 ,SVR和MAP继续下降 ;PVR继续下降 ,MPA无明显变化 ,PCWP、CVP有所升高 ,回输血后各指标均有所回升。整个稀释过程中 ,心搏血量SV和心搏指数SVI,左室每搏作功指数LVSWI、右室每搏作功指数RVSWI的变化与CO(CI)的变化相似。结论 :中度、深度ANHD时 ,机体代偿 ,CO(CI)及SV(SVI)升高 ,SVR、PVR降低 ,有利于循环 ;极深度ANHD时 ,CO(CI)及SV(SVI)不再升高反而降低 ,MAP下降明显 ,MPA无明显变化 ,对体循环影响大 ,对肺?     

一氧化氮对急、慢性缺氧大鼠血流动力学及缺氧性肺血管收缩反应的影响

观察了吸入０．００４％的一氧化氮（ＮＯ）对急、慢性缺氧大鼠血流动力学、缺氧性肺血管收缩反应（ＨＰＶ）、血气及高铁血红蛋白（ＭｅｔＨｂ）的影响。结果表明：（１）常氧吸入ＮＯ时能明显降低慢性缺氧大鼠肺动脉平均压（Ｐｐａ）和肺血管阻力（ＰＶＲ），但对正常大鼠的Ｐｐａ和ＰＶＲ无明显影响；（２）慢性缺氧大鼠急性缺氧时ＨＰＶ较正常大鼠弱，吸入ＮＯ不但降低两者的急性缺氧肺动脉高压，且完全逆转两者的ＨＰＶ；（３）吸入ＮＯ对急、慢性缺氧大鼠体循环血流动力学、血气及ＭｅｔＨｂ含量无明显影响。提示吸入ＮＯ能选择性降低，急、慢性缺氧性肺动脉高血压，且逆转ＨＰＶ。     

超声检测腺苷急性血管扩张试验在先天性室间隔缺损合并重度肺动脉高压中的应用

目的应用彩色多普勒超声心动图评价腺苷急性血管扩张试验在先天性室间隔缺损合并重度肺动脉高压中的应用价值。方法选取先天性室间隔缺损并重度肺动脉高压患者68例,肘静脉注射腺苷75μg.kg-1.min-1,应用彩色多普勒超声心动图检测主动脉平均压、肺动脉平均压、肺动脉收缩压、肺动脉舒张压、肺血管阻力、体循环阻力、肺循环阻力/体循环阻力比值、肺动脉/主动脉收缩压比值、肺循环/体循环血流量比值,注射5min后,与用药前比较,肺血管阻力下降>30%和肺动脉平均压下降>10%为急性血管扩张试验阳性。结果肺动脉平均压、肺动脉压、肺血管阻力、肺循环阻力/体循环阻力比值、肺动脉/主动脉收缩压比值用药后较用药前均明显下降(P<0.05),肺循环/体循环血流量比值明显增加(P<0.05),体循环阻力下降不明显(P>0.05),其中39例急性血管扩张试验阳性者,通过临床药物降压治疗有效后成功手术;29例急性血管扩张试验阴性者继续降压治疗无效并非手术适应证而行保守和对症治疗。结论腺苷选择性作用于肺血管,在先天性室间隔缺损合并重度肺动脉高压中,是急性血管扩张试验中较好的药物。     

Effects of 3, 4-Dihydroxyacetophenone on Cytosolic Calcium in Pulmonary Artery Endothelial and Smooth Muscle Cells during Acute Hypoxia

Summary: The effects of 3, 4-Dihydroxyacetophenone (3. 4-DHAP) on cytosolic free calcium[Ca^2 ], in pulmonary artery endothelia (PAECs) and smooth muscle cells (PASMCs) during acute hypoxia were studied. Porcine pulmonary artery endothelial and smooth muscle cells (PASMCs)were cultured primarily, and they were divided into 4 groups: groups incubated under normoxia or hypoxia and those with or without treatment with 3. 4-DHAP. The [Ca^2 ]i of both PAECs and PASMCs was measured by determining the fluorescence of fura 2 AM on spetrofluorometer. Our results showed that hypoxia caused significant elevation of [Ca^2 ]i. in both PAECs and PASMCs,3, 4-DHAP could attenuate the hypoxic elevation of [Ca^2 ]i only in PASMCs but not in PAECs. It is concluded that 3, 4-DHAP decreases the hypoxic elevation of [Ca^2 ]i in PASMCs. This might contribute to its inhibitory effect on hypoxic pulmonary vasoconstriction.     

Inhibition of Expression of Hypoxia-inducible Factor-la mRNA by Nitric Oxide in Hypoxic Pulmonary Hypertension Rats

Summary In order to study the effect of nitric oxide (NO) on the expression of hypoxia-inducible factor-1 alpha (HIF-1α) mRNA in hypoxic pulmonary hypertension (HPH) rats, 30 healthy male Wistar rats were randomly divided into normoxic control group, chronic hypoxic group and hypoxia plus L-argine (L-Arg) group. The animal model of HPH was developed. The mean pulmonary arterial pressure (mPAP) was measured by inserting a microcatheter into the pulmonary artery. The HIF-1α mRNA expression levels were detected by in situ hybridization (ISH) and semiquantitative RT-PCR. It was found that after 14 days hypoxia, the mPAP in normoxic control group (17.6±2.7 mmHg,l mmHg=0.133 kPa) was significantly lower than that in chronic hypoxic group (35.8 ±6.1 mmHg,t=0.2918,P<0.05) and mPAP in chronic hypoxic group was higher than that in hypoxia plus L-argine group (24.4±3.8 mmHg,t=0.2563,P<0.05). ISH showed that the expression of HIF-1α mRNA in the intraacinar pulmonary arteriolae (IAPA) in normoxic control group (0.1076±0.0205) was markedly weaker than that in chronic hypoxic group (0.3317±0.0683,t=3.125,P<0.05) and that in chronic hypoxic group was stronger than that in hypoxia plus L-argine group (0.1928±0.0381,t=2.844,P<0.05). RT-PCR showed that the content of HIF-1α mRNA in chronic hypoxic group (2.5395±0.6449) was 2.16 times and 1.75 times higher than that in normoxic control group (1.1781±0.3628) and hypoxia plus L-argine group (1.4511±0.3981), respectively. It is concluded that NO can reduce the mPAP by the inhibition of the expression of HIF-1α mRNA, which may be one of the mechanisms through which NO affects the pathogenesis of HPH. AO Qilin, male, born in 1971, Lecturer, M. D., Ph. D. This project was supported by a grant from National Natural Sciences Foundation of China (No. 39730190).     

Role of sympathetic nerve and adrenal gland in the potentiation of hypoxic pulmonary vasoconstriction during cigarette smoking

Summary The aim of this study was to investigate the role of sympathetic nerve and adrenal gland in the changes in hemodynamics and hypoxic pulmonary vasoconstriction (HPV) induced by cigarette smoking in Wistar rats. Data showed that an increment of pulmonary vascular resistance (PVR) induced by alveolar hypoxia was partially prevented by prazosin (α₁-receptor blocker) and prazosin combined with propranolol (β-receptor blocker), but not significantly affected by propranolol or adrenalectomy. Cigarette smoking tould increase PVR and potentiate HPV, whereas the potentiation of HPV was partially inhibited by prazosin and strengthened by propranolol, but not affected by adrenalectomy. It is suggested that the sympathetic nerve excitation caused by cigarette smoking may play a role in the strengthening of HPV.     

Effect of tetrandrine on expression of bFGF in lung tissue of rat with chronic hypoxic pulmonary hypertension]

OBJECTIVE: To evaluate effect of tetrandrine (Tet) on chronic hypoxic pulmonary by observing expression of bFGF in lung tissue of rat with chronic hypoxic pulmonary hypertension. METHODS: 30 rats were divided into 3 groups: hypoxia group, hypoxia + Tet group and control group. On the 21st day of hypoxia, the hemodynamic parameters were measured and the intra acinar pulmonary arteries (IAPA) changes were studied under light microscopes. Immunohistochemical staining with monoclonal antibody against human recombinant bFGF was performed in the paraffin section of rat lung. RESULTS: Tetrandrine could reduce pulmonary artery mean pressure (mPAP) and pulmonary vascular resistance (PVR), (P < 0.01), decrease the expression of bFGF in wall of IAPA, and inhibit hypoxic structural remodeling of IAPA. CONCLUSION: Tetrandrine could decrease the expression of bFGF in wall of IAPA and in that connection to inhibit remodeling of IAPA and pulmonary hypertension.     

汉防已甲素对慢性低氧性肺动脉高压大鼠肺内bFGF表达的影响

目的　观察汉防已甲素 ( tetrandrine,Tet)对慢性低氧大鼠肺组织碱性成纤维细胞生长因子( b FGF)表达的影响 ,探讨 Tet降低低氧性肺动脉高压的机制。方法　建立低氧性肺动脉高压大鼠模型 ,检测血流动力学 ,观察肺腺泡内动脉 ( IAPA)结构的变化 ,用免疫组化法检测肺组织 b FGF表达。结果　 Tet能明显降低肺动脉压 ,抑制肺腺泡内动脉壁 b FGF表达 ,阻抑肺腺泡内动脉重建。结论　 Tet通过抑制 b FGF表达 ,从而阻抑肺腺泡内动脉重建是其降低肺动脉压的机制之一