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目的 调查河南、山东部分地区家猫汉赛巴尔通体血清抗体及菌血症情况.方法 间接酶联免疫吸附试验(ELISA)方法调查314份家猫血清抗体情况,通过聚合酶链反应(PCR)和测序对血培养产物进行病原学鉴定.结果 用ELISA法共检测314份标本,总的抗体阳性率为29.6%,<6月龄的幼猫阳性率较低(19.3%),>2岁的老猫抗体阳性率最高(37.5%),雌性和雄性之间没有明显差别,血培养共培养出42份可疑阳性,PCR及序列分析证实为汉赛巴尔通体.结论 河南、山东部分地区家猫均存在汉赛巴尔通体感染情况,汉赛巴尔通体是家猫感染的主要菌种. 相似文献
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目的建立巴尔通体的基质辅助激光解吸电离飞行时间质谱(MALDI-TOF MS)数据库和质谱鉴定方法,利用野生菌株进行验证、评价。方法应用MALDI-TOF MS采集巴尔通体ATCC标准菌株和国内流行菌株的质谱数据,每株菌采集24张的质谱图集,获得特定的蛋白指纹图谱,汇总成不同种巴尔通体的标准质谱图,建立巴尔通体MALDI-TOF MS标准数据库。比较乙醇-甲酸提取法和直接涂抹法对质谱鉴定结果的影响,并进行重复性实验评估方法的稳定性。进一步应用173株野生巴尔通体菌株评估该标准数据库和相应的质谱鉴定方法。结果应用汉赛巴尔通体、五日热巴尔通体和杆菌样巴尔通体等21种/亚种巴尔通体,共计200株多地区来源的地理菌株,建立了巴尔通体MALDI-TOF MS蛋白指纹图谱标准数据库。2种样品制备方法均可以达到正确鉴定标准,提取法质谱得分略高于直接涂抹法。应用8种、16株巴尔通体菌株进行的重复性验证,得分为(9.078±0.031)~(9.776±0.006)分,变异系数(CV)为0.06%~1.12%。14种、173株巴尔通体测试菌株的质谱鉴定得分为9.014~9.796分,平均(9.462±0.195)分,在种水平上能被正确识别。结论基于该研究自主建立的巴尔通体质谱数据库,MALDI-TOF MS技术能够快速、准确鉴定巴尔通体种类,该课题组所建立的巴尔通体质谱标准数据库具有重要应用价值,对临床上巴尔通体病的实验室检验与诊断具有重要意义。 相似文献
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目的 调查云南省德宏傣族景颇族自治州(德宏州)居住区啮齿动物的巴尔通体感染和分布特点,为相关疾病防制提供参考依据。方法 采用笼夜法捕鼠,采集鼠类脾脏标本,用胰酶大豆琼脂培养基分离巴尔通体,用聚合酶链式反应法扩增巴尔通体gltA基因片段,并进行核苷酸序列测定和系统发育分析。结果 2011年在德宏州居住区共捕获啮齿动物5属9种340只和食虫动物2种18只,黄胸鼠为优势种(77.09%,276/358)。这些动物的脾脏标本中巴尔通体分离率为24.86%(89/358),其中黄胸鼠分离率为28.99%(80/276),其他阳性鼠种为黑缘齿鼠(5株)、社鼠(1株)、大绒鼠(1株)、小泡灰鼠(1株)和臭鼩鼱(1株)。共获得85株巴尔通体gltA基因核苷酸序列,进化分析表明,40株为特利波契巴尔通体(Bartonella tribocorum),28株为伊丽莎白巴尔通体(B.elizabethae),11株为昆州巴尔通体(B.queenslandensis),3株为森林巴尔通体(B.silvatica),3株未定种。黄胸鼠携带上述所有种类的巴尔通体。结论 德宏州啮齿动物中至少存在4种巴尔通体的流行,巴尔通体具有遗传多样性特点。以特利波契巴尔通体、伊丽莎白巴尔通体和昆州巴尔通体为主要流行菌株,黄胸鼠为当地居住区巴尔通体的主要宿主。今后应加强巴尔通体相关疾病的监测和防制工作。 相似文献
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目的优化巴尔通体体外药敏试验方法,检测多种抗生素对巴尔通体的最低抑菌浓度(MICs),并分析耐药性。方法优化Etest法试验条件;将该方法与琼脂稀释法进行比较,评价该方法的可靠性。采用Etest法,检测了7株ATCC参考株对22种抗生素的MICs,数据分析使用SPSS 13.0软件。结果确定含5%羊血的胰酶大豆琼脂培养基为最佳培养基、2.0 MCF(麦氏浊度)度为最佳细菌接种浓度,7天为最佳孵育时间。测试的7株巴尔通体在体外对强力霉素等13种抗生素敏感(MIC0.016),对克林霉素等4种抗生素MIC偏高。结论 Etest法简便易行、定值准确、重复性好、结果可靠,可以用于巴尔通体体外药敏试验,并获得了巴尔通体的一些体外药敏数据,为将来制订巴尔通体药敏标准提供一定的依据。 相似文献
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目的 检测巴尔通体菌株对10类19种抗生素最低抑菌浓度(MICs),分析药物敏感性及耐药性,为指导临床用药和耐药监测提供实验和数据参考。方法 采用E试验法,检测巴尔通体属11种、35株菌株对强力霉素、阿奇霉素、利福平等19种抗生素的MICs。将培养的巴尔通体菌制成McFarland(MCF)2.0浊度的菌悬液,均匀涂布于含5%去纤维羊血的胰酶大豆琼脂培养基上,置5% CO2的37℃培养箱培养。培养5~7 d后判读MICs。结果 35株巴尔通体菌在体外对强力霉素、阿奇霉素、红霉素、克拉仙霉素等13种抗生素敏感,MICs 0.016 mg/L;34株对利福平敏感,MICs 0.002 mg/L;对克林霉素、丁胺卡那霉素、万古霉素、多粘菌素和磺胺类5种抗生素不敏感,MICs较高。结论 绝大多数巴尔通体菌对强力霉素等14种抗生素敏感,但也对克林霉素等5种抗生素不敏感,在对巴尔通体病进行治疗时要注意选择敏感药物。 相似文献
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产气荚膜梭菌是自然界常见的致病菌,广泛分布于人类和动物的胃肠道、食品和环境中,已被美国、欧盟部分国家及日本列为食源性疾病暴发的主要原因之一。 近年来产气荚膜梭菌感染在全球呈上升趋势。 目前该菌的分子流行病学方面研究较多,常用的分子分型方法包括脉冲场凝胶电泳、多位点序列分型、核心基因多位点序列分型、单核苷酸多态性分析、毒素分型等。 分子分型技术对研究产气荚膜梭菌的暴发溯源、流行情况、遗传演化规律与种群特征具有重要意义,同时分子分型方法的不断创新与改进能够提高产气荚膜梭菌感染检测与诊断的有效性、准确性。 相似文献
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Maggi RG Chomel B Hegarty BC Henn J Breitschwerdt EB 《Molecular and cellular probes》2006,20(2):128-134
Since the isolation of Bartonella vinsonii subspecies berkhoffii from a dog with endocarditis in 1993, this organism has emerged as an important pathogen in dogs and as an emerging pathogen in people. Current evidence indicates that coyotes, dogs and gray foxes potentially serve as reservoir hosts. Based upon sequence differences within the 16S-23S ITS region and Pap31 gene, we propose a classification scheme that divides B. vinsonii subsp. berkhoffii isolates into four distinct types. Two conserved sequences, of 37 and 18 bp, respectively, are differentially present within the ITS region of each of the four B. vinsonii subsp. berkhoffii types. To date, B. vinsonii berkhoffii types I, II, and III have been identified in the US, type III in Europe and type IV in Canada. Based upon the proposed genotyping scheme, the geographic distribution of B. vinsonii berkhoffii types needs to be more thoroughly delineated in future molecular epidemiological studies involving Bartonella infection in coyotes, dogs, gray foxes, human beings and potentially other animals or in arthropod vectors. Strain typing may help to better define the reservoir potential, carriership patterns, modes of transmission, and geographic distribution for each B. vinsonii berkhoffii type. 相似文献
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A new in situ hybridization technique was developed for identification of Bartonella henselae cells in cell suspension or in tissue sections. Use of highly specific probes labeled with either fluorescein or digoxigenin allows discrimination between B. henselae and closely related B. quintana cells. No cross-hybridization with other Bartonella or non-Bartonella species was observed. Besides its specificity it showed higher sensitivity as compared to PCR based detection methods. Moreover, its application allows direct observation of B. henselae in infected tissues. 相似文献
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McCool TL Hoey JG Montileone F Goldenberg HB Mordechai E Adelson ME 《Diagnostic microbiology and infectious disease》2008,60(1):17-23
The antibody response to Bartonella henselae has been studied in a number of mammals; however, the human response needs to be further studied. After natural infection, humans have antibody reactivity to a large number of B. henselae proteins. We used a proteomic approach to identify antigenic proteins of B. henselae to determine their capacity to elicit a human antibody response. Comparing patient sera by Western blot analysis demonstrated significant amounts of reactivity to B. henselae. The immunofluorescence assay (IFA)-positive sera identified several protein spots of interest. However, a consistent reactivity to a single spot by all sera was not observed. Three of these spots demonstrated reactivity in 71%, 64%, and 64% of positive sera tested with negligible reactivity to the negative sera. These proteins were identified as GroES, BepA, and GroEL. Most IFA-positive sera demonstrated reactivity to GroES, GroEL, and BepA. The usefulness of these proteins for a clinical serologic assay is discussed. 相似文献