丙烯氨氧化催化剂物化性能研究：Ⅱ.催化剂失活前后的结构表征

采用ＸＲＤ，ＩＲ，Ｍｏｂａｕｅｒ ＥＳＲ，ＸＰＳ，ＳＥＭ和孔结构分析等分析测试手段，对比研究了新鲜，失活和再生三种丙烯氨氧化催化剂的体相结构和表面性质。结果表明，新鲜催化剂中各元素以Ｆｅ２（ＭｏＯ４），α－ＣｏＭｏＯ４，ＮｉＭｏＯ４，γ－Ｂｉ２Ｏ３．ＭｏＯ３，α－Ｂｉ２Ｏ３．３ＭｏＯ３，η－ＭｏＯ３，α－Ｂｉ２Ｏ３和β－Ｂｉ２Ｏ３形式存在２；失活催化剂中，部分Ｆｅ２（ＭｏＯ４）３转变为α－Ｆｅ－Ｍ     

THE PEAK LATENCY OF ORBITAL PRESACCADIC SPIKE POTENTIAL WITH HORIZONTAL EYE MOVEMENTS

ＴＨＥＰＥＡＫＬＡＴＥＮＣＹＯＦＯＲＢＩＴＡＬＰＲＥＳＡＣＣＡＤＩＣＳＰＩＫＥＰＯＴＥＮＴＩＡＬＷＩＴＨＨＯＲＩＺＯＮＴＡＬＥＹＥＭＯＶＥＭＥＮＴＳＳｈａｎＹａｎｇ；（单扬），ＭａｒｋＬ．ＭｏｓｔｅｒａｎｄＲｉｃｈａｒｄＡ．Ｒｏｅｍｅｒ（Ｄｅｐａｒｔ...     

CONSTRUCTION AND APPLICATION OF HUMAN CHROMOSOMAL SPECIFIC DNA PROBE P

ＣＯＮＳＴＲＵＣＴＩＯＮＡＮＤＡＰＰＬＩＣＡＴＩＯＮＯＦＨＵＭＡＮＣＨＲＯＭＯＳＯＭＡＬＳＰＥＣＩＦＩＣＤＮＡＰＲＯＢＥＰＯＯＬＳＬｉＬｕｙｕｎ李麓芸Ｉｎ１９８３－１９８６，ｔｗｅｎｔｙ－ｆｏｕｒｈｕｍａｎｃｈｒｏｍｏｓｏｍａｌｔｙｐｅｓｗｅｒｅｃｏ...     

银杏叶提取物对牛主动脉平滑肌细胞的增殖的影响

目的：观察银杏叶提取物（ＥＧＢ）对小牛主动脉平滑肌细胞（ＳＭＣ）增殖的影响。方法：分别采用体外培养的的ＳＭＣ及经氧化修饰型低密度脂蛋白造成ＳＭＣ增殖,选用３～８代ＳＭＣ,（１）分为对照组和ＥＧＢ组。（２）分为对照组和ｏｘｌｄｌ（０．１ｍｇ／ｍｌ）组,在ｏｘｄｌ组中加入个浓度ＥＧＢ（０,５０,１００,２００,４００,４００μｇ／ｍｌ）,培养２４ｈ后用ＭＴＴ和＾３Ｈ－ＴｄＲ掺入法测定细胞含量及其培养液中ＳＯＤ、ＬＰＯ、ＴＸＢ２、ＰＧＩ２。结果：ＥＧＢ后可使细胞ＭＴＴ含量和＾３Ｈ－ＴｄＲ掺入量减少,其抑制作用与剂量相关。ｏｘｌｄｌ可使ＳＭＣ明显增殖,加用ＥＧＢ后可使ＳＭＣ增殖受到抑制。ＥＧＢ组与ｏｘｌｄｌ组相比,ＳＯＤ、ＰＧＩ２升高,ＬＰＯ、ＴＸＢ２降低。结论：ＥＧＢ可直接抑制ＳＭＣ增值,并呈剂量依赖型。ＥＧＢ抑制ｏ     

95' QINGDAO INTERNATIONAL CONGRESS ON ORTHOPAEDICS

９５＇ＱＩＮＧＤＡＯＩＮＴＥＲＮＡＴＩＯＮＡＬＣＯＮＧＲＥＳＳＯＮＯＲＴＨＯＰＡＥＤＩＣＳＣｏ－ｓｐｏｎｓｏｒｅｄｂｙｔｈｅＣｈｉｎｅｓｅＳｏｃｉｅｔｙｏｆＯｒｔｈｏｐｅｄｉｃＳｕｒｇｅｏｎｓｏｆｔｈｅＣｈｉｎｅｓｅＭｅｄｉｃａｌＡｓｓｏｃｉａｔｉｏ...     

THE ASSOCIATION OF POLYMORPHISMS AT A VNTR LOCUS 3’ TO THE APOLIPOPROTEIN B GENE WITH CORONARY HEART DISEASE IN CHINESE POPUL

ＴＨＥＡＳＳＯＣＩＡＴＩＯＮＯＦＰＯＬＹＭＯＲＰＨＩＳＭＳＡＴＡＮＴＲＬＯＣＵＳ３’ＴＯＴＨＥＡＰＯＬＩＰＯＰＲＯＴＥＩＮＢＧＥＮＥＷＩＴＨＣＯＲＯＮＡＲＹＨＥＡＲＴＤＩＳＥＡＳＥＩＮＣＨＩＮＥＳＥＰＯＰＵＬＡＴＩＯＮＹｅＰｉｎｇ（叶平），ＣｈｅｎＢ...     

成骨生长肽的合成及药效学的实验研究

作者利用固相法合成了成骨生长肽 (ＯＧＰ)。并在体外观察了ＯＧＰ在低剂量范围对原代成骨细胞的促增殖作用 ,以及成骨活性标志蛋白ＡＫＰ的表达水平[1] 。在ＳＤ大鼠体内观察了ＯＧＰ对低钙饮食造成的骨质疏松的药效作用。现报道如下。一、材料和方法1 材料 :(1)试剂 :所有Ｂｏｃ 氨基酸、Ｂｏｃ Ｇｌｙ ＰＡＭ树脂购自美国ＡＢＩ公司 ;Ｅ ＭＥＭ培养基、胰蛋白酶 ＥＤＴＡ(依地酸 ) [0 .5 % (ｗ/ｖ)胰蛋白酶 ,5 .3ｍｍｏｌ/ＬＥＤＴＡ 4Ｎａ]为ＧＩＢＣＯＢＲＬ公司产品 ;Ⅱ型胶原酶为日本和光纯药工业株式会社产品 ;ＭＴＴ为Ｆｌｕｋａ…     

CASE REPORTS Koshevnikov syndrome in apatient with MELAS plus syndrome:electron microscopic and neuroimage studies

ＣＡＳＥＲＥＰＯＲＴＳＫｏｓｈｅｖｎｉｋｏｖｓｙｎｄｒｏｍｅｉｎａｐａｔｉｅｎｔｗｉｔｈＭＥＬＡＳｐｌｕｓｓｙｎｄｒｏｍｅ：ｅｌｅｃｔｒｏｎｍｉｃｒｏｓｃｏｐｉｃａｎｄｎｅｕｒｏｉｍａｇｅｓｔｕｄｉｅｓＴｈａｊｅｂＰｅｔｅｒｕｓ戴道恩，ＨｕａｎｇＫｏ...     

ONCOGENIC TRANSFORMATION OF SYRIAN HAMSTER EMBRYO CELLS BY 5．3－MeV α PARTICLES AND A TUMOR PROMOTER PHORBOL ESTER

ＯＮＣＯＧＥＮＩＣＴＲＡＮＳＦＯＲＭＡＴＩＯＮＯＦＳＹＲＩＡＮＨＡＭＳＴＥＲＥＭＢＲＹＯＣＥＬＬＳＢＹ５．３－ＭｅＶαＰＡＲＴＩＣＬＥＳＡＮＤＡＴＵＭＯＲＰＲＯＭＯＴＥＲＰＨＯＲＢＯＬＥＳＴＥＲＳｈｏｕＪｉａｎｇ（寿江）；ＧｏｎｇＹｉｆｅｎ（龚诒芬）...     

髓系过氧化物酶基因表达对急性白血病分型的意义

目的：比较基因分型和免疫分型在急性白血病（ＡＬ）诊断分型中的意义。方法：用光敏生物素标记髓系过氧化物酶（ＭＰＯ）ｃＤＮＡ探针,以链亲和素－胶体金细胞原位杂交（ＩＳＨ－ＳＡＧ）研究了５４例ＡＬ ＭＰＯｍＲＮＡ基因表达,并同时分析了ＡＬ的免疫表型。结果：１３例急性淋巴细胞白血病（ＡＬＬ）有１例表达ＭＰＯ基因;所有髓系均不同程度的表达ＭＰＯ基因,３例急性髓系白血病（ＡＭＬ）－Ｍｏ均表达ＭＰＯ基因。结论：ＭＰＯｍＲＮＡ敏感性强,对确认ＡＭＬ－Ｍｏ有重要意义,将基因分型和免疫分型结合分析,对ＡＬ更精确的诊断分型有重要意义。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.     

Scientific principles and rigorous processes should be followed in developing clinical guidelines for therapeutic interventions of integrative medicine

A clinical guideline for the therapeutic interventions of integrative medicine may be defined as a written document which states a series of recommendations on therapeutic interventions of integrative medicine for a special disease or condition. The guideline may provide assistance to medical professionals in making clinical decisions aimed at improving the clinical outcome of patients and reducing the costs of medical care（~＇4~. Recommendations issued by a guideline should be based on the best available evidence in both Western and Chinese medicine. For fulfilling this purpose, the development of clinical guidelines for therapeutic interventions in the field of integrative medicine should follow scientific principles and undergo a rigorous processes.