UF-100全自动尿液流式细胞分析仪检测唾液有形成分的初步研究

目的：探索全自动尿液流式细胞分析仪（UF－100）用于唾液分析的可行性。方法：对稀释唾液检测进行了初步的评价实验。包括批内重复性试验、线性范围测定以及红细胞（RBC）回收实验。结果：重复性实验的变异系数（CV）值大多在15％以下，RBC浓度范围50－2500个/μl，白细胞（WBC）30－1800个／μl，上皮细胞（EC）10－400个／μl，细菌（BACT）400－28000个／μl，平均相关系数（r）分别是RBC0．99，WBC0．99，EC0．99，BACT0．98。不同标准浓度的RBC平均回收率为101．6％，98．8％。不同时间、不同温度下的RBC回收率有 明显差异。结论：初步实验说明UF－100是一种高精度、高效率的全自动分析仪，可用于唾液中有形成分的检测。     

间质巨噬细胞释放的细胞因子在肺纤维化形成中的动态变化

目的：动态观察博莱霉素致肺纤维化大鼠间质巨噬细胞（IM）释放IL－1和TNFα。方法：酶消化法分离间质巨噬细胞，生物活性法测定IL－1和TNFα。结果：博A来霉素注入后肺泡巨噬细胞（AM）分泌IL－1未发生变化，而M则在第1天出现短暂的分泌高峰（280．24U／10^IM),AM和IM释放的TNFα都在第3天明显升高（AM33．33％，IM22．01％），第7天达高峰，AM的分泌峰值明显高于IM（AM69．13％，IM46．72％），结论：AM和IM释放细胞因子的功能存在一定差异，它们在肺纤维化中可能发挥不同的作用。α     

全自动干化学法测定血清胆碱酯酶的方法学探讨

目的：对全自动干化学法测定血清胆碱酯酶进行应用与评价。方法：在VITROS250干式生化分析仪上测定血清胆碱酯酶，并同时在日立7170全自动生化分析仪上检测。结果：血清胆碱酯酶的检测限为200-11500U／L；高、中、低浓度的批内CV分别为2．2％、3．1％和4．2％，，批间CV分别为2．8％、3．6％和4．8％；回收率为98．8％-103．2％；与日立7170全自动生化分析仪（X）相关系数r=0．992．相关方程Y=0．986X＋12；血红蛋白〈2．0g／L，胆红素浓度〈356μmol／L，甘油三酯浓度〈7．8mmol／L，对本法测定基本无干扰。结论：此法测定胆碱酯酶快速准确可靠。     

罗氏cobas e601测定SF的方法学性能评价

目的：对过保修期的老旧罗氏Cobas e601全自动电化学发光免疫分析仪检测血清铁蛋白（SF）的性能进行验证。方法：参照CLSI EP15－A2、EP9－A2文件及ISO15189实验室认可的要求，对SF的批内精密度、批间精密度、准确度、线性范围、参考区间进行验证实验。结果：批内精密度高低两个浓度的（CV）分别为1．41％和1．32％，日间精密度高低两种浓度SF分别为3．70％和2．98％；当月卫生部室间质评SF与靶值偏倚均合格；验证了厂商提供的测量线性范围；验证了厂商提供的参考区间。结论：本罗氏CobasE601检测SF结果准确可靠，仍然能够满足临床检测的要求。     

大肠癌患者泵注5－氟尿嘧啶持续化疗的临床观察及护理

目的：探讨和评价对大肠癌患者治疗中，采用泵注5－氟尿嘧啶（5－Fu）行持续化疗的临床效果和护理体会。方法：选择2013年2月～2014年6月在我院接受治疗的110例大患者为研究对象，采用泵注5－氟尿嘧啶进行持续化疗，护理过程采用优质护理方式。结果：患者化疗护理前癌胚抗原（CEA）、血清 CA199、CA195含量分别为（35．6±10．7）ng／ml、（569．6±217．5）ku／L、（667．5±128．4）ku／L，化疗护理后为（21．8±6．5）ng／ml、（246．5±93．8）ku／L、（365．7±169．2）ku／L，前后比较差异具有统计学意义（P＜0．05）；患者化疗护理后出现并发症10例，占总数9．09％；患者及其家属对护理工作满意度为98．18％。结论：大肠癌患者使用泵注5－氟尿嘧啶化疗效果显著，采用优质护理临床效果佳，值得进一步推广和借鉴。     

加替沙星治疗急性下呼吸道感染的临床评价

目的：采用病例对照研究的方法评价加替沙星的治疗急性下呼吸道感染的临床疗效和安全性。方法：经临床确诊为急性下呼吸道感染的患者108例，随机分为治疗组和对照组，其中治疗组56例，口服加替沙星0．4g，每日1次；对照组口服环丙沙星0．2g，每日2次，疗程均为7－14d，所有病例治疗结束后随访7d，结果：治疗组和对照组的痊愈率分别为89．29％（50／56）和73．08％（38／52），有效率分别为98．21％（55／56）和80．77％（42／52），两组细菌清除率分别为100％（57／57）和82．61％（38／46），两组间临床和细菌学疗效有显著性差异（均P＜0．05），治疗组和对照组不良事件发生率分别为10．71％（6／56）和7．69％（4／52）（P＞0．05），且均为消化道反应。结合：加替沙星抗菌谱广，抗菌活性强，能安全有效地治疗急性下呼吸道感染。     

产前诊断取材手术及并发症分析(附381例报告)

目的：探讨改进产前诊断取材手术方法，并对手术并发症进行分析。方法：绒毛活检（CVS）采用改良法。羊膜穿刺采用经腹徒手法及B超下穿刺法。结果：（1）CVS213例，一次手术成功率为92．96％（198／213）；二次手术成功率为97．65％（208／213）；（2）羊膜穿刺术168例，一次手术成功率为90．48％（152／168）；二次手术成功率为98．80％（166／168）。流产率：（1）CVS为2．35％（5／213）；（2）羊膜穿刺术为0。出血率。（1）CVS为3．28％（7／213）；（2）羊膜穿刺术为12．50％（21／168）。感染率均为0。结论：CVS及羊膜腔穿刺术，只要在正确把握手术时机的基础上，掌握娴熟的技术和技术技巧。是提高手术成功率，降低手术并发症的关键。     

RP—HPLC法测定双黄连注射液中黄芩苷的含量

目的建立高效液相色谱法测定双黄连注射液中黄芩苷外标定量的方法。方法采用RP—HPLC法，色谱柱为Kromasil C18（150×4．6mm，5μm）；流动相：甲醇-1％冰醋酸（50：50），流速为1ml／min，紫外检测波长为280nm。结果黄芩苷的线性范围为3．772～94．30μg／ml；相关系数为0．9993；加样回收率为95．74％；检测限为25．7ng；精密度实验RSD为1．7％；重现性实验RSD为1．94％；稳定性实验RSD为1．87％；三批样品中黄芩苷的含量为11．94mg／ml，13．47mg／ml，16．43mg／ml。结论该方法简便、快速、准确、灵敏度高、重现性好，可用于该制剂的含量测定。     

潮汕地区献血者和血液透析病人丙型、乙型肝炎病毒的感染状况

采用EIA法对318名潮汕地区职业献血者及37名血液透析（血透）病人进行血清抗－HCV及乙型肝炎病毒标记物检测；结果抗－HCV献血者阳性率5．7％（18／318）；血透病人为51．4％（19／37），正常人为0．7％（10／1414），乙型肝炎病毒感染标记物阳性，献血员阳性率13．5％（43／318），血透病人为35．1％（13／37），正常人为26．8％（379／1414）。     

WIN 55,212-2对培养的大鼠三叉神经节神经元胞内游离钙离子浓度的影响

目的检测WIN55，212—2对培养的大鼠三叉神经节神经元细胞内钙离子浓度（[Ca^2＋]i）的影响并探讨其机制。方法以Fura一2／AM为钙探针，用细胞内双波长钙荧光系统检测细胞[Ca^2＋]i的变化。结果WIN55，2122（0．01～10μmol／L）可浓度依赖性地升高三叉神经节神经元细胞内[Ca^2＋]i，EC50为0．47μmol／L；用大麻素Ⅰ型受体（CBI受体）阻断剂AM251孵育后，发现10μmol／L的AM251可明显抑制10μmol／L WIN55。212—2对三叉神经节神经元细胞内[Ca^2＋]i的作用（P〈0．01）。结论WIN55，212—2可浓度依赖性地升高三叉神经节神经元细胞内[Ca^2＋]i，该作用可能是由CBI受体所介导。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.