清肠化湿方对小鼠溃疡性结肠炎模型结肠黏膜上皮细胞Caspase-3、ZO-1的影响

目的以核因子-κB(nuclear facter-κB,NF-κB)p65反义寡核苷酸(ASODN)及西药柳氮磺胺吡啶(SASP)为对照,观察清肠化湿方对三硝基苯磺酸(TNBS)法诱导小鼠溃疡性结肠炎(UC)模型结肠黏膜天冬氨酸特异酶切的半胱氨酸蛋白酶-3(Caspase-3)、闭锁小带蛋白-1(ZO-1)表达的影响。方法采用TNBS诱导小鼠UC模型,并随机分为清肠化湿方组、SASP组、清肠化湿方+SASP组、ASODN组、模型对照组、空白对照组,治疗7 d后处死小鼠,取小鼠结肠标本,采用HE染色法观察各组小鼠结肠病理形态,并采用Western blot法检测各组小鼠结肠上皮细胞Caspase-3及ZO-1蛋白表达水平。结果模型对照组小鼠Caspase-3蛋白表达明显高于空白对照组(P0.05);清肠化湿方组Caspase-3蛋白表达低于模型对照组(P0.05),与空白对照组比较差异无统计学意义(P0.05)。模型对照组小鼠ZO-1蛋白表达明显低于空白对照组(P0.05);清肠化湿方组ZO-1蛋白表达高于模型对照组(P0.05),与空白对照组比较差异无统计学意义(P0.05)。结论清肠化湿方对UC模型小鼠具有治疗作用,其抑制肠上皮细胞Caspase-3蛋白表达、增强ZO-1的表达,抑制肠黏膜组织细胞凋亡,恢复正常的肠黏膜屏障形态和功能可能是其作用机制的一部分。     

消溃方灌肠治疗溃疡性结肠炎模型大鼠的作用机制研究

目的研究消溃方对溃疡性结肠炎(UC)模型大鼠TH17/Treg平衡靶点的调节作用,对FOXP3、RORγt的表达和细胞因子白细胞介素-17(IL-17)、白细胞介素-6(IL-6)、白细胞介素-10(IL-10)和TGF-β表达的影响。方法通过三硝基苯磺酸-乙醇灌肠法建立溃疡性结肠炎大鼠模型,分别采用美沙拉秦灌肠液(0.7 g/kg)和消溃方高、低剂量(18.3、9.15 g/kg)进行灌肠治疗;治疗完成后,采用流式细胞术检测大鼠外周血Treg和Thl7细胞占总CD4^+T细胞百分比,酶联免疫吸附法(ELISA)分析大鼠血清中IL-17、IL-6、IL-10和TGF-β水平,Western blot方法检测结肠组织中Treg转录因子FOXP3和Th17转录因子RORγt的表达水平。结果美沙拉秦组和消溃方高、低剂量组治疗后均能够降低促炎因子IL-17、IL-6的浓度,提高IL-10和TGF-β的浓度,且消溃方高、低剂量组和美沙拉秦组均能提高FOXP3的表达,降低RORγt的表达,达到抗炎、促进UC溃疡面愈合的作用,并且消溃方高剂量组效果优于消溃方低剂量组和美沙拉秦组。结论消溃方能促进溃疡性结肠炎大鼠结肠组织中Treg细胞分化生成,介导免疫耐受程度,抑制Th17细胞分化,调节Th17/Treg平衡,维持体内免疫平衡,减轻肠道炎症反应,从而起到治疗溃疡性结肠炎的作用。     

肺炎链球菌3型多糖对支气管哮喘小鼠气道炎症及Treg/Th17细胞的影响

目的观察肺炎链球菌3型多糖(T3P)对支气管哮喘小鼠气道炎症以及Treg/Th17细胞的影响,探讨T3P对哮喘小鼠的免疫调节作用。方法 BALB/c小鼠随机分成对照组、哮喘组和T3P组,每组8只。哮喘组以卵清蛋白(OVA)致敏、气道激发建立哮喘模型,对照组以PBS代替,T3P组在OVA致敏前皮下注射T3P进行干预。观察小鼠肺组织病理改变,对支气管肺泡灌洗液(BALF)进行细胞计数及分类,ELISA法检测血清OVA特异性IgE(OVA-IgE)以及BALF中白介素4(IL-4)、干扰素γ(IFN-γ)、IL-10、IL-17浓度,实时荧光定量PCR法检测肺组织中转录因子Foxp3和RORγt mRNA表达水平,流式细胞术检测Treg和Th17细胞占CD4~+细胞百分比。结果 T3P组小鼠肺组织炎症反应程度弱于哮喘组,血清OVA-IgE,BALF中细胞总数、嗜酸粒细胞比例以及IL-4、IL-17浓度均低于哮喘组(P<0.05),IFN-γ、IL-10浓度高于哮喘组(P<0.05),小鼠肺组织Foxp3 mRNA表达水平及Treg占CD4~+细胞百分比均高于哮喘组(P<0.05),RORγt mRNA表达水平和Th17细胞占CD4~+细胞百分比均低于哮喘组(P<0.05)。哮喘组和T3P组小鼠肺组织Foxp3-mRNA/RORγt-mRNA比值及Treg/Th17细胞比例与BALF中嗜酸粒细胞数量呈负相关。结论 T3P可以通过抑制过敏原特异性IgE表达调节Th1/Th2、Treg/Th17细胞平衡,抑制气道炎症。     

白头翁汤对溃疡性结肠炎患者Th17/Treg细胞失衡调节作用

目的:观察白头翁汤对溃疡性结肠炎患者Th17/Treg细胞失衡的调节作用,以探讨其治疗UC的可能机制。方法:将40例UC患者随机分为中药治疗组和西药对照组,分别给予白头翁汤内服外灌及口服美沙拉嗪缓释颗粒。采用流式细胞术检测UC患者外周血中Th17细胞、Treg细胞占总CD4+T细胞百分比并计算Th17/Treg比率,采用Western blot检测结直肠组织RORγt和Foxp3蛋白的表达。结果:白头翁汤治疗后Th17细胞比例及Th17/Treg较治疗前明显减少(P0.01),较西药对照组减少(P0.05);Treg细胞比例较治疗前明显增加(P0.01),较西药对照组增加(P0.01)。白头翁汤治疗后能下调RORγt表达(P0.01),上调Foxp3表达(P0.01)。结论:白头翁汤对溃疡性结肠炎患者有明显的治疗作用,其作用机制可能与提高患者Treg细胞比例、降低Th17细胞比例,进而恢复机体Th17/Treg平衡,抑制肠道炎症有关。     

白细胞介素-17对小鼠胚胎丢失的作用

目的:探讨正常妊娠与自然流产小鼠体内Th17/Treg细胞的表达差异以及白细胞介素-17(IL-17)对正常妊娠小鼠胚胎丢失的影响。方法:孕4 d时分别给予正常妊娠小鼠(CBA/J♀×BALB/c♂)生理盐水和IL-17A构建正常妊娠组和给药组,给予自然流产小鼠(CBA/J♀×DBA/2♂)生理盐水构建自然流产组。孕14 d时计算胚胎吸收率,采用ELISA法测定外周血IL-17水平,Real-time PCR及Western blot法检测胎盘组织IL-17、RORγt及Foxp3的表达水平。结果:自然流产组及给药组胚胎吸收率、胎盘组织中IL-17、RORγt的mRNA及蛋白水平显著高于正常妊娠组,而Foxp3水平则低于正常妊娠组;自然流产组外周血IL-17含量显著高于正常妊娠组。结论:自然流产小鼠体内Th17/Treg平衡明显向Th17细胞偏移,IL-17是介导流产发生的独立危险因素之一。     

清肠化湿方对TNBS大鼠结肠组织PPAR-γ/p38 MAPK的影响

目的 :观察清肠化湿方(QHD)对三硝基苯磺酸(TNBS)诱导的大鼠结肠炎结肠组织中过氧化物酶体增殖物激活受体(PPAR-γ)和p38丝裂原活化蛋白激酶(p38 MAPK)表达的影响,探讨其治疗溃疡性结肠炎(UC)的可能作用机制。方法:采用80只Wistar大鼠建立UC大鼠模型,随机分为8组,分别为空白对照组、模型对照组、QHD低剂量组、QHD中剂量组、QHD高剂量组、柳氮磺胺吡啶(SASP)组、柳氮磺胺吡啶+双酚A-二甘氨酸醚(SASP+BADGE)组及中药中剂量+BADGE组,每组10只。除空白对照组以生理盐水灌肠外,其余组采用TNBS/乙醇灌肠造UC大鼠模型。以柳氮磺胺吡啶(SASP)为阳性对照,同时联合使用PPAR-γ抑制剂双酚A-二甘氨酸醚(BADGE),给药7 d后病理检测各组大鼠结肠组织的损伤情况,并通过蛋白质印迹法(Western blot)和实时荧光定量多聚核苷酸链式反应(Real-time PCR)实验分别检测大鼠结肠组织中PPAR-γ、p38 MAPK的蛋白和基因表达,同时检测各组大鼠结肠组织中黏蛋白2(MUC2)和三叶因子3(TFF3)的表达情况。结果:清肠化湿方可上调TNBS大鼠结肠组织PPAR-γ的表达(P<0.01),同时抑制p38 MAPK的活性(P<0.01),并促进结肠组织中MUC2和TFF3表达(P<0.01),当联用PPAR-γ抑制剂BADGE后其对p38 MAPK的抑制作用减弱(P<0.05)。结论:清肠化湿方对TNBS大鼠结肠炎有明显的保护作用,其作用机制可能与激活PPAR-γ信号通路、抑制p38 MAPK的激活、减轻炎症反应、升高结肠组织中MUC2与TFF3的表达水平、参与肠黏膜的修复有关。     

栀子苷对类风湿性关节炎大鼠Th17/Treg平衡和局部炎症因子的影响

目的考察栀子苷对类风湿性关节炎大鼠Th17/Treg平衡和局部炎症因子的影响。方法建立大鼠胶原诱导关节炎模型,通过病理切片观察踝关节组织变化,ELISA检测血清TNF-α,Western blot法检测转录因子Foxp3和ROR-γt的蛋白表达,免疫组化法检测关节滑膜组织IL-10、IL-17表达。结果模型组血清TNF-α、IL-17以及ROR-γt和关节滑膜IL-17表达较正常组显著升高(P0.01),而模型组血清IL-10浓度以及Foxp3和关节滑膜IL-10表达较正常组显著降低(P0.01),栀子苷中、高剂量组血清TNF-α、IL-17浓度以及ROR-γt和关节滑膜IL-17表达较模型组显著降低(P0.05~0.01),而血清IL-10浓度、Foxp3和关节滑膜IL-10表达较模型组显著升高(P0.05~0.01)。结论大鼠胶原诱导关节炎模型成模之后Treg/Th17会失衡,栀子苷可升高IL-10、Foxp3表达,降低IL-17、ROR-γt表达,提示其可能通过诱导Treg细胞生成同时抑制Th17细胞分化,从而恢复病变部位Treg/Th17的平衡。     

补中益气颗粒对EAT大鼠Treg/Th17细胞因子表达的影响

目的探讨补中益气颗粒对实验性自身免疫性甲状腺炎(EAT)大鼠调节性T细胞(Treg)/辅助性T细胞17(Th17)细胞因子表达的影响,从Treg/Th17细胞因子失衡角度探讨其治疗桥本氏甲状腺炎的机制。方法运用猪甲状腺球蛋白与完全弗氏佐剂和不完全弗氏佐剂混合免疫注射法及高碘喂养法复制EAT大鼠模型,随机分为空白组、模型组、补中益气组、优甲乐组、补中益气+优甲乐组,药物干预2个月后,取大鼠脾脏组织,采用荧光定量PCR、蛋白质印迹(Western blot)法分别检测大鼠脾脏转化生长因子-β(TGF-β)、叉头翼状螺旋转录因子(Foxp3)、维甲酸相关孤儿受体γT(RORγT)、白细胞介素-17(IL-17)基因和蛋白表达水平。结果与空白组比较,模型组RORγT、IL-17基因和蛋白表达上调(P0.05),Foxp3、TGF-β基因表达减少(P0.05),蛋白表达无统计学意义(P0.05)。与模型组比较,补中益气组、优甲乐组、补中益气+优甲乐组RORγT、IL-17基因表达下调(P0.05),蛋白表达减少,RORγT改变具有统计学意义(P0.05);Foxp3、TGF-β基因表达上调(P0.05),蛋白表达无统计学意义(P0.05)。药物各组组间比较,仅优甲乐组Foxp3蛋白表达高于补中益气组(P0.05),RORγT、IL-17、TGF-β蛋白和基因表达3组间无统计学意义。结论补中益气颗粒能够抑制甲状腺自身免疫性炎症,其作用机制可能与调节Treg/Th17相关细胞因子的平衡状态有关。     

壮骨止痛胶囊对去卵巢骨质疏松大鼠骨组织Treg／Th17平衡的调节作用

目的从骨组织调节性T细胞/辅助性T细胞(Treg/Th17)平衡角度探讨壮骨止痛胶囊抗绝经后骨质疏松作用机理。方法 72只8月龄SD雌鼠随机分为假手术组,模型组,戊酸雌二醇组(0.21 mg/kg),壮骨止痛胶囊高剂量组(1.28 g/kg)、中剂量组(0.64 g/kg)和低剂量组(0.32 g/kg),每组12只。除假手术组外,其余5组去卵巢复制绝经后骨质疏松动物模型。术后1周开始给药,连续给药18周,最后一次给药1 h后处死,快速取左股骨检测雌二醇(E2)、白细胞介素17A(IL-17A)、白细胞介素10(IL-10),取右股骨RT-PCR检测维甲酸相关孤体受体γt(RORγt)、叉状头转录因子(Foxp3)、IL-17A、IL-10 mRNA的表达,取左胫骨病理切片免疫组化染色检测表达RORγt、Foxp3的细胞百分数。结果壮骨止痛胶囊能显著升高去卵巢雌鼠骨组织E2、IL-10水平(P0.01),降低骨组织IL-17A水平(P0.05);显著增强骨组织Foxp3、IL-10 mRNA的表达(P0.05),显著抑制骨组织RORγt、IL-17A mRNA的表达(P0.01或P0.05);显著增加骨组织内表达Foxp3的细胞百分数(P0.05),下调表达RORγt的细胞百分数(P0.05)。Foxp3阳性细胞百分数与RORγt阳性细胞百分数比值与E2水平呈正相关(r=0.990 5,P0.01)。结论壮骨止痛胶囊通过抑制去卵巢雌鼠骨组织Th17细胞数量和功能活动,增加骨组织Treg细胞数量并增强其活动而调节骨组织Treg/Th17平衡,该调节作用可能部分通过提高去卵巢雌鼠体内E2水平而实现。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

Development on Adjustable Calibration Marker for Shock Wave Focus

Shock wave lithotripsy （SWL） is a treatment of choice for upper urinary stones. However, this procedure is inappropriate for obese patients because the focus is often unable to reach the target owing to the limited focal distance in shock wave source. Although treating such patients in a blast path may increase the application length of shock wave source, it＇s difficult to find this path on the lithotripter monitor. For this reason, we invented an adjustable calibration marker in order to set an effective focus in the shock wave hath.     

Study on the TCM Pathogenesis of Angiopathic Complications of Type II Diabetes

Excess production of reactive oxygen species(ROS)of mitochondrion mediated by hyperglycemia is the common pathogenesis of angiopathic complications of diabetes.TCM holds that the damp from the dysfunction of spleen.kidney and liver is the causative factor of complications of diabetes.This is similar to the mechanism of Ros resulting in angiopathic complications of diabetes.When the angiopathic complications of type II diabetes mellitus(T2DM)are difierentiated as caused by turbid damp in TCM can be explained as ROS.Since the obstruction of pathogenic damp in channels and collaterals is said to be the main pathogenesis,the treating principle should be dissolving the damp to remove the obstruction.     

Leptin, Ghrelin and TNF-α before and after Hypo-caloric Traditional Chinese Diet and Auricular Acupuncture

INTRODUCTION Obesity is a complex emergent problem, which can be possibly solved not only by the diet but also by the life style and promotion of a constant physical exercise. 1, 2 No doubt careful attentions must be given to the nutritional condition of obese people, the dietary habits, the somatic build (i.e. distribution of fat mass) and the organic functions linked to formation of the fat mass. All the parameters should be constantly monitored before, during and after a diet treatment. 3, 4, 5     

Relationship between Dysglycemia and Carotid Atherosclerosis in Tibetan Population

People with dysglycemia are at high risk for atherosclerotic diseases. This study aims at investigating the atherosclerotic vascular damage in dysglycemia and its metabolic origin in Tibetan population.     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.