浅谈如何加强医院行政机关建设与管理

如何加强医院机关行政科室的建设与管理，强化行政工作人员在医院管理年活动中的参与意识、责任意识，激发其工作的主动性和创造性，是在医院管理年活动中不断探讨、实践并努力完善的重要工作内容之一。     

Effects of transfection of ICAP-1α and its mutants on adhesion and migration of 2H-11 cells

This study examined the effect of integrin cytoplasmic domain-associated protein 1α (ICAP-1α) and its mutatants T38A and I138A on the adhesion, migration and tube formation of 2H-11 cells.rAAV-ICAP-1α, rAAV-T38A and rAAV-I138A were constructed.After infection, the expression of ICAP-1α and p-ERK1/2, p-c-Jun protein was measured by Western blotting.Adhesion ability was evaluated by using MTT.Cell migration was determined by using Boyden chamber method.Tube formation test was conducted on Matrigel.The results showed that in ICAP-1α, T38A and I138A groups, ICAP-1α protein expression was increased.In T38A and I138A groups, phospho-ERK1/2, phospho-c-Jun protein expressions were significantly increased as compared with the control group and the GFP group.ICAP-1α group protein expression was obviously decreased when compared with the control group and the GFP group.Cell adhesion ratio was 0.1429±0.0080 in control group, 0.1434±0.0077 in GFP group and the ratio in T38A and I138A groups increased to 0.3210±0.0082 and 0.3250±0.0079, respectively.In ICAP-1α group, the ratio was decreased to 0.1005±0.0073.In T38A and I138A groups, the number of migrating 2H-11 cells was increased to 31.45±3.20 and 33.10±5.40 against 18.51±2.80 in control group and 20.47±3.12 in GFP group.In ICAP-1α group, the number was decreased to 12.06±1.72.The number of tube-like structures was increased to 20.41±2.54 in T38A and to 22.26±3.07 in I138A groups as compared to those of control group 12.45±1.84 and GFP group 13.63±2.71.In ICAP-1α group, the number of tube-like structures was decreased to 8.32±1.24.It was suggested that rAAV-T38A and rAAV-I138A transfection can substantially increase 2H-11 cell adhesion, migration and angiogenisis, while rAAV-ICAP-1α can greatly inhibit the effect.These effects might be correlated with ERK1/2 and c-Jun protein phosphorylation.     

长蛇灸对强直性脊柱炎患者血清基质金属蛋白酶3及基质金属蛋白酶组织抑制因子1的影响

目的：观察长蛇灸对早中期强直性脊柱炎患者血清基质金属蛋白酶3（MMP-3）和基质金属蛋白酶组织抑制因子1（TIMP-1）的影响。方法：将60例早中期强直性脊柱炎患者随机分为两组,治疗组30例,采用长蛇灸进行治疗;对照组30例,口服柳氮磺胺吡啶治疗。观察两组患者治疗前后血清MMP-3和TIMP-1水平的变化。结果：两组患者治疗后血清MMP-3明显下降（P〈0·01~0·05）,但治疗组下降更显著,与对照组比较,有极显著性差异（P〈0·01）;两组患者治疗后血清TIMP-1明显升高（P〈0·01）,但治疗组升高更显著,与对照组比较,有显著性差异（P〈0·05）。结论：长蛇灸治疗早中期强直性脊柱炎,可减少致炎因子的释放,调节异常的基质降解和减少血管形成等炎症的病理因素,缓解AS炎症、减少软骨和骨质破坏。     

2型糖尿病患者下肢动脉病变的影响因素

选择T2DM患者90例,用糖尿病足诊断箱中双向多普勒血流仪检测糖尿病患者的双侧胫后动脉压、足背动脉压、肱动脉血压。计算踝肱比值,ABI小于0.9提示PAD存在。结果ABI0.9有17例,占18.89%。其中ABI0.7有5例,占5.56%;双侧ABI0.9有8例,占8.89%;单纯右侧ABI0.9有5例,占5.56%;单纯左侧ABI0.9有4例,占4.45%。与年龄、糖尿病病程及高血压病程呈现显著相关(P值0.05),与性别无明显相关性(P值0.05)。PAD与HbA1c、HDL-C呈相关(P值0.05),与TC、TG、LDL-C无明显相关性(P值0.05)。结论:多普勒(Doplix)超声检查测定ABI能客观准确显示PAD情况,T2DM PAD发生率较高,影响PAD发生的可能因素有年龄、糖尿病病程及高血压病程、HbA1c、HDL-C。     

长蛇灸对强直性脊柱炎患者外周血T淋巴细胞亚群的影响

目的观察长蛇灸对早中期强直性脊柱炎患者外周血T淋巴细胞亚群的影响,进一步阐明长蛇灸治疗早中期强直性脊柱炎的免疫学机制。方法将60例早中期强直性脊柱炎患者随机分为2组,治疗组30例,采用长蛇灸进行治疗;对照组30例,口服柳氮磺胺吡啶治疗。运用流式细胞(FCM)技术检测2组患者治疗前、后外周血CD4+、CD8+细胞百分率及CD4+/CD8+比值。结果治疗后2组CD4+、CD4+/CD8+均呈升高趋势,但治疗组显著升高,与治疗前比较有显著性差异(P均<0.05),与对照组比较有显著性差异(P均<0.05)。治疗后2组CD8+含量均显著下降(P均<0.01),但治疗组明显降低,与对照组比较有显著性差异(P<0.05)。结论长蛇灸治疗早中期强直性脊柱炎,能有效调节患者免疫功能,改善免疫功能低下的状态。