目的:探讨高迁移率蛋白1( high lobility group box 1, HMGB1)在糖尿病大鼠视网膜中的表达及其可能机制。 方法:将SD大鼠60只随机分为糖尿病组和对照组。采用STZ腹腔注射制作糖尿病大鼠模型,糖尿病组大鼠腹腔注射STZ 60lg/kg,对照组腹腔注射同等剂量的生理盐水。分别于1、2和4lo时处死大鼠,摘取视网膜,HE染色观察视网膜结构变化,荧光血管造影观察视网膜血管变化, TUNEL染色观察视网膜细胞凋亡情况, Western Blot检测视网膜中HMGB1和NF-κB的表达。 结果:与对照组相比,糖尿病组大鼠视网膜各层细胞排列紊乱,细胞密度减低,可见微血管病变,以及内、外核层变薄和神经节细胞的凋亡;荧光血管造影见周边毛细血管迂曲,可见血管闭塞及无灌注区;Western Blot 检测HMGB1和NF-κB表达呈时间依赖性增高,且两者表达呈正相关(P<0.05)。 结论:HMGB1在糖尿病大鼠视网膜中表达增加, HMGB1可能通过NF-κB信号通路参与了糖尿病视网膜病变的发生。 相似文献
Journal of Natural Medicines - Two new clerodane diterpenoids (1 and 2), a new pyran-2-one derivative (3), along with five known compounds (4?8), were isolated from Croton... 相似文献
Background In-stent restenosis is a common complication after stent implantation. However, the assessment of stent lumen in computed tomography (CT) coronary angiography is limited by multiple factors. Our study aimed to evaluate the accuracy and the suspected affecting factors in diagnosing coronary in-stent restenosis by dual-source CT (DSCT) compared with coronary angiography.
Methods One hundred and fifteen stents in 50 patients were evaluated with DSCT before coronary angiography for the detection of coronary in-stent restenosis (≥ 50% luminal narrowing). Patency of each stent was analyzed by two independent expert radiologists blinded to the results of coronary angiography. The relationship between diagnostic accuracy and the suspected factors including age, body mass index (BMI), heart rate, variation of heart rate, radiation dose, image quality, location and stent characteristics (type, material, diameter, length and strut thickness) was assessed with both univariate and multivariate analysis. The fitting of a Logistic regression model was evaluated using a receiver operating characteristic (ROC) curve.
Results Mean stent diameter was (2.9±0.4) mm. Sensitivity, specificity, positive and negative predictive values and accuracy of DSCT in detection of in-stent restenosis were 69.2%, 91.2%, 50.0%, 95.9%, and 88.7%, respectively. In a subgroup of stents with a diameter ≥3.0 mm, sensitivity, specificity, positive and negative predictive values and accuracy were 100.0%, 96.5%, 75.0%, 100.0%, and 96.8%, respectively. Stent diameter <3.0 mm and poor image quality were associated with poor diagnostic accuracy (P <0.05). The area under curve of ROC was 0.79.
Conclusion DSCT can provide high accuracy for the assessment of in-stent restenosis in stents with a diameter ≥3.0 mm, and can play an important role in ruling out in-stent restenosis. 相似文献
The objective of this study is to present a relatively simple method to reconstruct cerebral aneurysms as 3D numerical grids.
The method accurately duplicates the geometry to provide computer simulations of the blood flow. Initial images were obtained
by using CT angiography and 3D digital subtraction angiography in DICOM format. The image was processed by using MIMICS software,
and the 3D fluid model (blood flow) and 3D solid model (wall) were generated. The subsequent output was exported to the ANSYS
workbench software to generate the volumetric mesh for further hemodynamic study. The fluid model was defined and simulated
in CFX software while the solid model was calculated in ANSYS software. The force data calculated firstly in the CFX software
were transferred to the ANSYS software, and after receiving the force data, total mesh displacement data were calculated in
the ANSYS software. Then, the mesh displacement data were transferred back to the CFX software. The data exchange was processed
in workbench software. The results of simulation could be visualized in CFX-post. Two examples of grid reconstruction and
blood flow simulation for patients with internal carotid artery aneurysms were presented. The wall shear stress, wall total
pressure, and von Mises stress could be visualized. This method seems to be relatively simple and suitable for direct use
by neurosurgeons or neuroradiologists, and maybe a practical tool for planning treatment and follow-up of patients after neurosurgical
or endovascular interventions with 3D angiography. 相似文献
Eleven new polyketones named diaporthsins A–K (1–11) were isolated from the fermentation of Diaporthe sp. JC-J7. The chemical structures of compounds (1–11) were elucidated by spectroscopic methods including HRESIMS, 2DNMR, NMR and chemical methods. Compound 11 features an unusual acyclic polyketone–phenolic polyketone hybrid structure that integrates the characteristics of different fungal metabolites (cytosporone and multiplolide). Compound 3 was the only C12-polyketone obtained in this research. These new polyketones showed inhibitory activity on triglycerides (TG) in steatosis hepatocyte L-02 cells. Among them, compound 5 and (4E)-6,7,9-trihydroxydec-4-enoic acid displayed inhibitory activities on TG in steatotic L-02 cells with inhibition ratios of 26% and 21% at concentration of 5 μg mL−1; also, inhibition ratios of 8-O-acetylmultiplolide A and phomopsisporone A at concentration of 5 μg mL−1 were calculated to be about 24% and 16%, respectively, which were equivalent to the antihyperlipidemic activity of lovastatin. The preliminary structure–activity relationship indicated that acetyl at C-8 can increase the antihyperlipidemic activity of multiplolide A and the glycol ester and hydroxyl at C-6 can also increase the corresponding activity of diaporthsin B.Eleven new polyketones were isolated from Diaporthe sp. JC-J7, and some compounds indicated antihyperlipidemic activity.相似文献