排序方式: 共有12条查询结果,搜索用时 15 毫秒
1.
2.
The myocardial protection afforded by ischemic preconditioning(IPC) can alleviate ischemia-reperfusion injury in normal rat heart.However,this myocardial protection is seldom studied in the type 2 diabetic rat with myocardial ischemia disease.In this study,we aimed to evaluate the effects of ATP-sensitive potassium channels(KATP channels) on IPC in the isolated type 2 diabetic rat heart and the role of the sulfonylurea gliclazide.Methods Streptozotocin(STZ)-induced type 2 diabetic male Wistar rats with or without gliclazide(64 mg /kg body weight,orally) and age-matched non-diabetic control rats were used for all studies.The isolated hearts were perfused with Langendorff’s system under the constant flow,pressure and temperature conditions with Kreb’s-Henseleit solution(K-H).After 5 minutes of balance perfusion,these rats were randomly divided into six groups:non-diabetic control rats without IPC(CIR);non-diabetic control rats with IPC(CIP);diabetic rats without IPC(DIR);diabetic rats with IPC(DIP);gliclazide-treated diabetic rats without IPC(GIR);and gliclazide-treated diabetic rats with IPC(GIP).Groups CIR,DIR,and GIR were subjected to 30-min global ischemia and 60-min reperfusion for induction of ischemia /reperfusion injury.Groups CIP,DIP,and GIP were given three cycles of 5-min ischemia and 5-min reperfusion as IPC,and then ischemia /reperfusion injury program was implemented.Extent of ischemia /reperfusion injury was measured in terms of the release of lactate dehydrogenase(LDH),creatine kinase(CK),and creatin kinase-MB(CKMB) in coronary effluent.After perfusion,Kir6.2 and SUR2A mRNA expressions in the myocardial tissue were characterized by fluorescent quantitative real-time PCR method,and Kir6.2 and SUR2A protein expressions were assessed by immunohistochemistry.Result In non-diabetic control rats,the release of LDH,CK,and CK-MB in coronary effluent markedly decreased with IPC compared with No-IPC(P < 0.05),but not in diabetic rats.However,in gliclazide-treated diabetic rats,IPC-induced decrease in the release of LDH,CK,and CK-MB was restored compared with No-IPC(P < 0.05).The expressions of Kir6.2 both at mRNA and protein levels in CIP were significantly higher than those in CIR.There was no significant difference in the expression of Kir6.2 and SUR2A both at mRNA and protein levels between DIP and DIR.However,the expression of Kir6.2 both at mRNA and protein levels was significantly higher in GIP than in GIR.No significant difference was detected in the mRNA expression level of SUR2A between the six groups.The expression of SUR2A at protein level was significantly higher in CIP than in CIR and in GIP than in GIR.Conclusions The cardioprotective effect of IPC is abolished in the isolated type 2 diabetic rats compared with non-diabetic control rats.However,to some extent,gliclazide can improve the myocardial protection of IPC against ischemia /reperfusion injury,thus suggesting that it is mediated mainly by KATP channels at mRNA or protein level,which provides a basis for further investigating the effects of KATP channels on IPC. 相似文献
3.
4.
韩圆圆 《中国现代药物应用》2020,(2):207-209
目的探讨加味知柏地黄汤与小剂量甲巯咪唑联合方案对甲状腺功能亢进症(甲亢)的临床效果。方法 88例甲亢患者,根据治疗方式的不同分为对照组与观察组,各44例。对照组采用常规小剂量甲巯咪唑治疗,观察组采用加味知柏地黄汤与小剂量甲巯咪唑联合治疗,比较两组患者的治疗效果、不良反应发生情况及血清总三碘甲状腺原氨酸(TT3)、总甲状腺素(TT4)、促卵泡生成素(FSH)、游离三碘甲状腺原氨酸(FT3)、游离甲状腺素(FT4)、骨钙素(BGP)、降钙素(CT)水平变化情况。结果观察组治疗总有效率为95.5%,高于对照组的75.0%,差异有统计学意义(P<0.05)。治疗后,观察组患者TT3、TT4、FT3、FT4水平分别为(2.6±0.3)、(85.5±6.6)、(6.4±1.0)mmol/L、(17.6±2.2)mmol/L,低于对照组的(3.9±0.8)、(138.4±8.8)、(11.5±1.6)mmol/L、(29.4±3.3)mmol/L, FSH水平(2.2±0.4)mIU/L高于对照组的(1.5±0.2)mIU/L,差异有统计学意义(P<0.05);观察组患者BGP水平为(6.2±1.0)ng/mL, CT水平为(68.6±6.2)ng/mL,均低于对照组的(9.0±1.3)、(77.3±3.6)ng/mL,差异有统计学意义(P<0.05)。观察组不良反应发生率为6.8%,低于对照组的15.9%,但差异无统计学意义(P>0.05)。结论予以甲亢患者加味知柏地黄汤与小剂量甲巯咪唑联合方案治疗效果理想,可改善患者甲状腺功能及骨代谢情况,安全性较高,可推广应用。 相似文献
5.
目的 探讨死胎发生的原因,为临床预防死胎、降低死胎发生率提供一定依据.方法 使用郑州大学第一附属医院电子病历管理系统检索2019年1月1日至2021年7月1日于产科住院且出院诊断包含死胎的病例,共检索出123例.回顾性分析其临床资料,包括年龄、体质量、死胎发生时的孕周、妊娠次数、流产次数、既往死胎病史、孕妇合并症等.详细查看患者病历资料后找出最可能导致死胎的原因.结果 母体因素导致死胎46例,脐带因素导致死胎39例,胎儿及羊水因素导致死胎15例,胎盘因素导致死胎9例,不明原因死胎14例.结论 死胎原因复杂,母体因素及脐带因素为导致死胎的主要原因,加强妊娠期母体健康监测、关注胎动异常可能有利于预防死胎. 相似文献
6.
韩圆圆 《实用中医内科杂志》2009,23(4)
介绍李晓娟教授应用小柴胡汤医案三则:汗证,证属营卫不和,津液不固,治宜调和营卫,固摄止汗。郁证,证属热扰胸膈证,治宜和解少阳,清宣郁热。胁痛,证属少阳气机不疏证,治宜和解少阳,疏肝理气。指出:大凡邪入半表半里,以及少阳经气不疏引起自身和其它脏腑病变,或经循所过之处器官病变者,常用此方,而不必悉具少阳诸症。 相似文献
7.
目的:胎膜早破先露部未入盆衔接孕妇使用U型垫枕,确保其体位正确,提高其舒适度。方法:将2011年1月~2011年12月我产科106例胎膜早破孕妇使用我科自制的U型垫枕在责任护士的帮助下垫于孕妇腰臀下,使其臀部抬高15~30°,绝对卧床休息,保持外阴清洁。结果:无1例发生脐带脱垂,孕妇均顺利娩出健康胎儿,未发生母婴感染。结论:对胎膜早破先露部未入盆衔接孕妇使用U型垫枕,能够使孕妇在生理、心理上达到最愉快的状态或降低不愉快的程度,避免因为患者的不舒适感觉,反射性地排斥治疗的情况发生。 相似文献
8.
9.
目的探讨牙周基础治疗对侵袭性牙周炎的临床疗效。方法选取2011年5月—2014年5月收治的侵袭性牙周炎患者96例,根据炎性侵润程度分为局限型侵袭性牙周炎组(A组)和广泛型侵袭性牙周炎组(B组),A组40例,B组56例。对患者在接受牙周基础治疗前后不同时间点的牙齿临床附着丧失量、探诊深度、菌斑指数以及出血指数进行跟踪检测,总结临床疗效并对比治疗效果,计量资料采用t检验,P0.05为差异有统计学意义。结果 A组患者牙周基础治疗后1、3、6个月临床附着丧失量、探诊深度、出血指数以及菌斑指数[(2.49±1.35)、(1.27±0.72)、(1.26±0.66)、(2.15±1.95)、(1.32±1.37)、(1.33±1.44)mm、(0.84±0.57)、(0.75±0.47)、(0.79±0.45)、(1.12±0.82)、(0.85±0.77)、(0.45±0.52)]与治疗前[(3.39±1.72)、(3.37±1.99)mm、(2.05±1.08)、(2.12±0.67)]比较,差异均有统计学意义(均P0.05)。B组患者牙周基础治疗后1、3、6个月临床附着丧失量、探诊深度、出血指数以及菌斑指数[(2.35±1.40)、(2.27±1.18)、(2.08±1.09)、(2.51±2.51)、(2.49±2.23)、(1.30±2.16)mm、(0.99±0.91)、(0.96±0.68)、(1.05±0.65)、(1.00±0.55)、(0.98±0.86)、(0.45±0.44)]与治疗前[(3.77±2.11)、(3.97±2.59)mm、(2.65±1.61)、(2.92±0.78)]比较,差异均有统计学意义(均P0.05)。结论牙周基础治疗在侵袭性牙周炎的的处理中,对相关出血、临床附着丧失以及牙齿探诊深度等检测指标都有显著改善,临床疗效显著,对解决牙周炎炎性病变具有重要意义。 相似文献
10.
背景:多种引流方式可用于临床治疗胆管梗阻疾病,其中内镜下胆管引流术的应用逐渐增多。目的:比较内镜下不同胆管引流方式治疗胆管梗阻疾病的效果、并发症等。方法:选取2012年2月—2017年12月宣城市人民医院收治的75例行内镜逆行胰胆管造影术(ERCP)治疗的胆管梗阻患者,分为鼻胆管引流组和胆管支架组,比较两组引流成功率、引流效果、并发症情况,并分析ERCP术后胆管再梗阻的原因。结果:治疗前,胆管支架组TBIL、DBIL水平显著高于鼻胆管引流组(P0.01)。治疗后,两组引流成功率相比无明显差异,TBIL、DBIL水平较治疗前无明显差异。与治疗前相比,治疗后鼻胆管引流组ALT、AST、GGT显著降低(P0.05),AMS显著升高(P0.05);胆管支架组ALT、GGT、AMS均显著降低(P0.05)。两组并发症发生率无明显差异。ERCP术后胆管再梗阻原因主要为胆泥或泥沙样结石、块状结石、肿瘤侵犯等。结论:鼻胆管引流和胆管支架引流均可改善患者肝功能,缓解胆管梗阻症状,两种方法的引流效果和并发症方面无明显差异。 相似文献