扩髓髓内钉与锁定接骨板治疗股骨干骨折的疗效比较

目的观察股骨扩髓髓内钉（RIN）和锁定接骨板（LCP）治疗股骨干骨折患者的临床疗效。方法对50例股骨干骨折患者分别采用RIN内固定（25例）和LCP内固定（25例）治疗。对比两组患者手术时间、术中失血量、切口长度、住院时间、术后8周骨痂面积、功能评价情况。结果50例均获得随访,时间RIN组11～28个月、LCP组10～32个月。术中出血量、住院时间及Flynn功能评价两组差异均无统计学意义（P〉0．05）。RIN组手术时间较LCP组长、切口长度较LCP组短、术后第8周骨痂面积比LCP组大,差异均有统计学意义（P〈0．05）。结论RIN与LCP两种内固定方式均能取得良好的治疗效果,但RIN组术后骨痂形成较多,骨折愈合情况更为理想。     

成人股骨干骨折治疗进展

股骨干骨折是指股骨小转子以下至股骨髁以上部分股骨的骨折,是临床最常见的成人骨折之一,约占全身骨折的6%,多发于20～40岁青壮年,男女比例约为2.8 ∶ 1.股骨干骨折通常由高能损伤所致,30%患者伴有多发骨折、广泛软组织及脏器损伤,这些损伤会导致大量出血,严重时出现休克[1]..     

Wnt5a基因慢病毒的构建与RSC96雪旺细胞的感染研究

目的 构建大鼠Wnt5 a基因慢病毒并感染RSC96雪旺细胞,观察慢病毒感染情况及Wnt5 a表达情况.方法 设计大鼠Wnt5 a基因特异性RNAi序列,构建并包装成可感染细胞的慢病毒,感染RSC96雪旺细胞后通过Celigo观察细胞中荧光表达,并通过实时荧光定量聚合酶链反应(RT-qPCR)检测细胞中Wnt5 a m...     

固醇调节元件结合蛋白2在软骨细胞退变过程中的表达

背景：近期研究证实了固醇调节元件结合蛋白2基因在骨关节炎发生过程中起重要作用,但其具体发病机制尚未完全清楚。 目的：通过白细胞介素1β体外诱导关节软骨细胞退变,观察固醇调节元件结合蛋白2在软骨细胞退变过程中的表达变化。 方法：体外分离培养C57BL/6J小鼠关节软骨细胞,将第2代软骨细胞随机分为4组：对照组、白细胞介素1β 24,48,     72 h组。后3组细胞分别以10 μg/L白细胞介素1β干预细胞。 结果与结论：软骨细胞经白细胞介素1β刺激后呈肥大化表现,软骨细胞活性随白细胞介素1β刺激时间的延长而逐渐降低。与对照组相比,白细胞介素1β 24,48,72 h组软骨细胞中固醇调节元件结合蛋白2与固醇调节元件结合蛋白裂解激活蛋白 mRNA表达水平增加,而蛋白聚糖和Ⅱ型胶原mRNA表达水平降低。提示白细胞介素1β能抑制软骨细胞增殖和细胞重要基质成分表达,诱导其出现肥大化退行性改变,且在退变的过程中,固醇调节元件结合蛋白2表达逐渐上调,与软骨关键基因的表达呈负向变化关系。 中国组织工程研究杂志出版内容重点：组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松;组织工程     

Influences of IL-6R antibody on PMMA bone cement-mediated expression of OPG and RANKL in synovial fibroblasts

Effect of interleukin-6 receptor （IL-6R） antibody on polymethyl methacrylate （PMMA） bone cement-mediated expression of osteoprotegerin （OPG） and ：receptor activator of nuclear fac- tor-kappaB ligand （RANKL） in synovial fibroblasts was investigated. Synovial tissue obtained from to- tal knee arthroplasty was digested and cultured. Inverted microscope was employed to observe the synovial cells and immunocytochemistry （SABC method） staining was used to identify synovial fibro- blasts. This experiment was divided into three groups according to different culture media： PMMA group （75μg/mL PMMA bone cement particles）, IL-6R antibody group （10 ng/mL IL-6R antibody＋75 μg/mL PMMA bone cement particles）, and control group （no IL-6R antibody or PMMA bone cement particles）. Influence of IL-6R antibody and PMMA on proliferation of synovial fibroblasts was meas- ured by cell counting kit-8 （CCK-8）. ELISA method was used to measure OPG and RANKL levels in culture solution. Fluorescence quantitative real-time PCR （FQ-PCR） was used to detect the expression of OPG and RANKL mRNA. After three consecutive passages, more than 95% of the primary synovial cells became long spindle fibroblast-like cells. SABC staining results showed that the fibroblast-like cells were negative for anti-CD68 antibody and positive for anti-vimentin antibody, with brown madder stained. CCK-8 test demonstrated that the absorbance （A） value at 450 nm was significantly lower in IL-6R antibody group than in PMMA group and control group （P〈0.01）, but there was no statistically significant difference in A value at 450 nm between the control group and PMMA group （P〉0.05）. Re- suits of ELISA indicated that the expression of OPG was significantly higher in IL-6R antibody group than in PMMA group and control group （P〈0.01）. The expression of RANKL was inhibited （P〈0.05）, and the ratio of OPG/RANKL was significantly increased in IL-6R antibody group as compared with PMMA group and control group. There was no significant difference in the expression of OPG between control group and PMMA group （P〉0.05）, but the expression of RANKL was higher in PMMA group than in control group （P〈0.05）, and there was a significant difference in the ratio of OPG/RANKL be- tween them （P〈0.05）. Results of FQ-PCR revealed the expression of RANKL mRNA was significantly inhibited （P〈0.01） and the expression of OPG mRNA was significantly increased （P〈0.01） in IL-6R an- tibody group as compared with PMMA group and control group. The expression of RANKL mRNA was higher in PMMA group than in control group （P〈0.05）, but the expression of OPG mRNA had no sig- nificant difference between them （P〉0.05）. IL-6R antibody could significantly increase the expression of OPC~ but inhibit the expression of RANKL, which might provide a theoretical basis of molecular bi- ology for the prevention and treatment of aseptic loosening of prosthesis.