排序方式: 共有21条查询结果,搜索用时 15 毫秒
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一叶秋碱诱导人白血病HL—60细胞凋亡 总被引:4,自引:0,他引:4
目的 研究一叶秋碱能否诱导HL-60细胞凋亡,方法 用MTT法检测一叶秋碱对细胞增殖影响;应用流式细胞仪检测凋亡细胞数;采用琼脂糖凝胶电泳法观测DNA碎片,透射电镜观察凋亡的形态改变,结果:一叶秋碱5-80mg.L^-1能诱导HL-60细胞凋亡,电镜观察到典型的凋亡形态学改变,电泳呈现出阶梯状条带,流式细胞仪检测到凋亡率随剂量的增高而升高,MTT法示一叶秋碱抑制HL-60细胞增殖,并且呈时间,剂量 相似文献
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榄香烯诱导人白血病K562细胞凋亡及调控bcl—2蛋白的表达 总被引:23,自引:0,他引:23
目的:研究榄香烯的抗肿瘤作用机制。方法:抑制细胞增殖采用MTT法,用荧光显微镜观察细胞的形态学变化,DNA电泳,流式细胞仪技术检测DNA断裂,用流式细胞仪检测bcl-2蛋白的表达,结果:榄香烯65-520μmol.L^-1明显抑制K562细胞增殖,IC50(95%可信区间)为220(152-319)μmol.L^-1电泳可见DNA断裂形成的阶梯状条带,形态学上表现为染色体聚集,核固缩,断裂,而bc 相似文献
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丹参酮Ⅱ—A磺酸钠对内皮细胞和血小板体外表达粘附分子的作用 总被引:16,自引:0,他引:16
AIM: To study the action of tanshinone II-A sulfonate (Tan) on adhesion molecule expression by cultured endothelial cells and platelets. METHODS: Tumor necrosis factor alpha (TNF-alpha)-induced ICAM-1 expression on the cell surface and endothelial adhesivity toward HL-60 cells were studied using human umbilical vein endothelial cells (HUVEC). Thrombin-induced expression of platelet P-selectin was studied using human blood platelets. Adhesion molecule expression on the cell surface was measured by flow cytometry. The number of HL-60 cells adhering to the HUVEC monolayer was determined by liquid scintillation spectroscopy. RESULTS: Pretreatment of HUVEC with TNF-alpha significantly enhanced ICAM-1 expression and increased HL-60 cells adhesion to HUVEC from 4.6% +/- 0.7% to 30% +/- 6%. Tan (25-200 mumol.L-1) inhibited the effects of TNF-alpha in a concentration-dependent manner. Tan also inhibited the increase of P-selectin expression of thrombin-activated platelets in a concentration-dependent manner. CONCLUSION: Tan inhibited expression of adhesion molecules (ICAM-1, P-selectin) in HUVEC and in human blood platelets. 相似文献
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用麻醉大鼠冠状动脉前降支结扎造成急性心肌梗塞模型,观察了复方丹参胶囊对心肌梗塞的作用。复方丹参胶囊60、120mg/kg于冠脉结扎前ip,显著降低结扎后5h的心肌梗塞范围,并使血清磷酸肌酸激酶(CPK)活性明显降低,保护心肌SOD活性,减轻心肌超微结构的病理改变。提示复方丹参胶囊对实验性心肌梗塞具有明显的保护作用。这种作用可能与其抗心肌脂质过氧化有关. 相似文献