| 一叶秋碱诱导人白血病HL—60细胞凋亡 |
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| 引用本文： | 董宁征,周文轩.一叶秋碱诱导人白血病HL—60细胞凋亡[J].中国药理学报,1999,20(3):267-270. |
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| 作者姓名： | 董宁征 周文轩 |
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| 作者单位： | [1]苏州医学院 [2]香港保健协会 |
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| 摘 要： | 目的 研究一叶秋碱能否诱导ＨＬ－６０细胞凋亡，方法 用ＭＴＴ法检测一叶秋碱对细胞增殖影响；应用流式细胞仪检测凋亡细胞数；采用琼脂糖凝胶电泳法观测ＤＮＡ碎片，透射电镜观察凋亡的形态改变，结果：一叶秋碱５－８０ｍｇ．Ｌ＾－１能诱导ＨＬ－６０细胞凋亡，电镜观察到典型的凋亡形态学改变，电泳呈现出阶梯状条带，流式细胞仪检测到凋亡率随剂量的增高而升高，ＭＴＴ法示一叶秋碱抑制ＨＬ－６０细胞增殖，并且呈时间，剂量
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| 关 键 词： | 一叶秋碱 细胞凋亡 HL－60细胞 白血病 DNA断片 |
Securinine induced apoptosis in human leukemia HL-60 cells. |
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| N Z Dong,Z L Gu,W H Chou,C Y Kwok.Securinine induced apoptosis in human leukemia HL-60 cells.[J].Acta Pharmacologica Sinica,1999,20(3):267-270. |
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| Authors: | N Z Dong Z L Gu W H Chou C Y Kwok |
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| Institution: | Department of Pharmacology, Suzhou Medical College, China. |
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| Abstract: | AIM: To study whether securinine might induce apoptosis in human leukemia HL-60 cells. METHODS: Inhibition of proliferation was measured using MTT assay. The amount of apoptotic cells was measured by flow cytometry. DNA fragmentation was visualized by DNA agarose gel electrophoresis and the cellular changes were observed by electron microscope. RESULTS: Securinine 5-80 mg.L-1 elicited typical apoptosis morphological changes and DNA fragmentation in a concentration-dependent manner in HL-60 cells. Securinine inhibited HL-60 cell proliferation in a time- and concentration-dependent manner. The IC50 and 95% confidence limits were 27 (15-47) mg.L-1 after 12-h treatment with securinine. CONCLUSION: Securinine induced apoptosis in HL-60 cells. |
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