中医药治疗颞下颌关节紊乱病临床体会

目的：探讨中医药治疗颞下颌关节紊乱病临床诊治的经验体会。方法：采用中医药方法对我科8年来收治的79例颞下颌关节紊乱病病例进行治疗并观察疗效。结果：治愈73例,显效4例,有效1例,无效1例,总有效率98.7%。结论：颞下颌关节紊乱病的病因复杂,应结合病因,采用有针对性的中西医综合治疗。     

复治肺结核病人的治疗要点

在我国肺结核病人中复发涂阳病人约占20％，为尽快治愈这部分病人，使病人恢复健康，减少疾病的传播流行具有重要的意义。     

肿瘤坏死因子凋亡相关配体基因的克隆和表达

目的：克隆、表达和鉴定肿瘤坏死因子凋亡相关配体(TRAIL)．方法：从培养的人外周血淋巴细胞中提取总RNA，经RT-PCR获得TRAIL基因．将该基因克隆到pGEM-Teasy载体中，测序鉴定．将TRAIL基因插入pBV220表达载体中，42℃诱导表达4～5h，进行SDS-PAGE分析．免疫印迹法鉴定TRAIL蛋白的表达．结果：DNA测序证明，获得了TRAIL基因，其序列与GenBank中报道序列完全一致．SDSPAGE分析表明，TRAIL蛋白获得高效表达，分子质量为17ku，表达量约占菌体总蛋白的300k，．免疫印迹法鉴定显示，该蛋白可与鼠抗人TRAIL mAb产生阳性反应．结论：成功克隆和表达了TRAIL基因．     

血压水平及其影响因素的人群分布

目的 :探讨海港职工血压水平及其影响因素的人群分布 ,为心血管病防治提供科学依据。方法 :采用国际标准化的心血管病流行病学调查方法 ,调查 10 2 5 6名 18～ 5 4岁青岛港职工 ,男 7795例 ,女 2 46 1例。结果 :理想血压和正常血压男性为 5 8.6 % ,女性为 85 .2 %。 45～ 5 4岁职工中血压处在正常高值及高血压Ⅰ、Ⅱ、Ⅲ级水平的比例男性占 5 4.8% ,女性占 33 .2 %。经相关分析调整年龄后体重指数、腰臀围比值、腰围、心率与收缩压、舒张压均呈显著正相关 ,男性饮酒量与血压显著正相关。结论 :青岛港职工高血压患病率男性明显高于女性     

脑星形细胞瘤中TIP30、VEGF和CD34的表达及其相关性研究

Objective To investigate the expression and relationship of TIP30(HIV-1 Tat interactive protein 2), vascular endothelial growth factor (VEGF) and MVD (detected by CD34) in the angiogenesis of human brain astrocytomas. Methods Expression of TIP30, VEGF and CD34 in 19 cases of normal brain tissue and 71 cases of astrocytoma were immunohistochemically examined with Elivision plus two-step method. Results The positive expression of TIP30 could be seen in cytoplasm of neuroglial cells and neurons of 19 normal brain tissues. The positive expression rate of TIP30 in 71 cases of astrocytoma was 33.80 % (24/ 71). The positive expression rate of TIP30 in astrocytoma of different grades was 52 % for grade Ⅱ, 34.78 % for grade Ⅲ and 13.04 % for grade Ⅳ. The positive expression rate of TIP30 in high grade (Ⅲ+Ⅳ) of astrocytoma was found significantly lower than that in low grade(Ⅱ) (χ2=5.71, P <0.05); The expression of VEGF and MVD detected by CD34 in astrocytomas were higher than that in normal brain tissue and increased as the tumor grade increased; In astrocytoma, the negtive correlation was found between the expression of TIP30 and VEGF (r=-0.428, P<0.05); no correlation was found between TIP30 and MVD(r=-0.065, P 0.05); the positive correlation was found between VEGF and MVD(r=0.684, P<0.01). Conclusion The positive expression rate of TIP30 in normal brain tissue is significantly higher than that in astrocytoma. The positive expression rate of TIP30 significantly decreases as the pathological grade of the astrocytoma increases; The expression of TIP30 and VEGF is negatively correlated in astrocytoma.     

脑星形细胞瘤中TIP30、VEGF和CD34的表达及其相关性研究

Objective To investigate the expression and relationship of TIP30(HIV-1 Tat interactive protein 2), vascular endothelial growth factor (VEGF) and MVD (detected by CD34) in the angiogenesis of human brain astrocytomas. Methods Expression of TIP30, VEGF and CD34 in 19 cases of normal brain tissue and 71 cases of astrocytoma were immunohistochemically examined with Elivision plus two-step method. Results The positive expression of TIP30 could be seen in cytoplasm of neuroglial cells and neurons of 19 normal brain tissues. The positive expression rate of TIP30 in 71 cases of astrocytoma was 33.80 % (24/ 71). The positive expression rate of TIP30 in astrocytoma of different grades was 52 % for grade Ⅱ, 34.78 % for grade Ⅲ and 13.04 % for grade Ⅳ. The positive expression rate of TIP30 in high grade (Ⅲ+Ⅳ) of astrocytoma was found significantly lower than that in low grade(Ⅱ) (χ2=5.71, P <0.05); The expression of VEGF and MVD detected by CD34 in astrocytomas were higher than that in normal brain tissue and increased as the tumor grade increased; In astrocytoma, the negtive correlation was found between the expression of TIP30 and VEGF (r=-0.428, P<0.05); no correlation was found between TIP30 and MVD(r=-0.065, P 0.05); the positive correlation was found between VEGF and MVD(r=0.684, P<0.01). Conclusion The positive expression rate of TIP30 in normal brain tissue is significantly higher than that in astrocytoma. The positive expression rate of TIP30 significantly decreases as the pathological grade of the astrocytoma increases; The expression of TIP30 and VEGF is negatively correlated in astrocytoma.     

脑星形细胞瘤中TIP30、VEGF和CD34的表达及其相关性研究

Objective To investigate the expression and relationship of TIP30(HIV-1 Tat interactive protein 2), vascular endothelial growth factor (VEGF) and MVD (detected by CD34) in the angiogenesis of human brain astrocytomas. Methods Expression of TIP30, VEGF and CD34 in 19 cases of normal brain tissue and 71 cases of astrocytoma were immunohistochemically examined with Elivision plus two-step method. Results The positive expression of TIP30 could be seen in cytoplasm of neuroglial cells and neurons of 19 normal brain tissues. The positive expression rate of TIP30 in 71 cases of astrocytoma was 33.80 % (24/ 71). The positive expression rate of TIP30 in astrocytoma of different grades was 52 % for grade Ⅱ, 34.78 % for grade Ⅲ and 13.04 % for grade Ⅳ. The positive expression rate of TIP30 in high grade (Ⅲ+Ⅳ) of astrocytoma was found significantly lower than that in low grade(Ⅱ) (χ2=5.71, P <0.05); The expression of VEGF and MVD detected by CD34 in astrocytomas were higher than that in normal brain tissue and increased as the tumor grade increased; In astrocytoma, the negtive correlation was found between the expression of TIP30 and VEGF (r=-0.428, P<0.05); no correlation was found between TIP30 and MVD(r=-0.065, P 0.05); the positive correlation was found between VEGF and MVD(r=0.684, P<0.01). Conclusion The positive expression rate of TIP30 in normal brain tissue is significantly higher than that in astrocytoma. The positive expression rate of TIP30 significantly decreases as the pathological grade of the astrocytoma increases; The expression of TIP30 and VEGF is negatively correlated in astrocytoma.     

滨州部分地区脑卒中的流行趋势分析

为了减少脑卒中的发病，降低其死亡率，提高患者的生活质量，笔者自1974年开始对脑卒中患者发病的有关因素及死亡原因进行分析、研究。同时回顾了1974～2004年我院收治的10547例脑卒中病例资料进行对比性研究。     

1例苯磺酸左旋氨氯地平片致双踝部水肿报道

一、病例资料 患者，女，55岁，因：“双下肢踝部水肿30余天．”于2013年1月9日到我院就诊、该患既往有高血压病史12年。曾间断口服珍菊降压片、     

健康护理与心理护理应用于真菌性阴道炎患者护理中的效果观察及对患者生活质量的影响

目的:探究健康护理与心理护理应用于真菌性阴道炎患者护理中的临床疗效以及对患者生活质量的影响。方法:采用随机抽样法选择在本院接受治疗真菌性阴道炎患者开展研究(收集病例数80例),将其按照随机抽样法分为对照组、观察组,病例数各为40例。对照组患者给予常规护理模式,观察组患者实施健康护理和心理护理,对比护理效果。结果:观察组患者护理效果显著优于对照组,组间数据对比:P0.05。结论:健康、心理护理有助于提高真菌性阴道炎患者的临床疗效以及其生活质量,值得推荐。