还原型谷胱甘肽联合甘草酸二铵对腹部开放伤后海水浸泡犬肝功能损伤的治疗作用

Objective To investigate effect of reduced glutathione and diammonium glycyrrhizinate on the treatment of hepatic damage in experimental dogs following open abdominal injuries coupled with seawater immersion. Methods Twenty-four dogs with open abdominal injuries were randomly divided into 3 groups: the control group (given with simple observation after 1.5 h seawater immersion); the routine treatment group (given routine care and fluid transfusion after 1.5 h seawater immersion) ; the hepatic treatment group (given routine are, fluid transfusion and hepatic treatment as well, after 1.5 h seawater immersion). Each group consisted of 8 dogs. Blood samples were taken at different time points to measure total bilirubin (TB), alanine aminotransferase (ALT), aspartate aminotransferase (AST), lactate dehydrogenase (LDH), prothromin time (PT), endotoxin, tumor necrosis factor-α(TNF-α), interleukin-6 (IL-6), and pathological examination was also made at the same time. Results For the control group, contents of TB, ALT, AST and LDH increased significantly and survival time was less than 24 hours. Contents of TB, ALT, AST and LDH of the routine treatment group also increased significantly, but survival time of all the experimental animals was mere than 24 hours. Reduced glutathione and diammonium glycyrrhizinate could reduce the extent of lesion quite significantly 12～24 hours following open abdominal injuries. Conclusions Reduced glutathione and diammonium glycyrrhizinate seemed to have good effects on the treatment and prevention against hepatic damage induced by trauma coupled with seawater immersion. It could be used as a primary means for the treatment of such kind of hepatic damage.     

还原型谷胱甘肽联合甘草酸二铵对腹部开放伤后海水浸泡犬肝功能损伤的治疗作用

Objective To investigate effect of reduced glutathione and diammonium glycyrrhizinate on the treatment of hepatic damage in experimental dogs following open abdominal injuries coupled with seawater immersion. Methods Twenty-four dogs with open abdominal injuries were randomly divided into 3 groups: the control group (given with simple observation after 1.5 h seawater immersion); the routine treatment group (given routine care and fluid transfusion after 1.5 h seawater immersion) ; the hepatic treatment group (given routine are, fluid transfusion and hepatic treatment as well, after 1.5 h seawater immersion). Each group consisted of 8 dogs. Blood samples were taken at different time points to measure total bilirubin (TB), alanine aminotransferase (ALT), aspartate aminotransferase (AST), lactate dehydrogenase (LDH), prothromin time (PT), endotoxin, tumor necrosis factor-α(TNF-α), interleukin-6 (IL-6), and pathological examination was also made at the same time. Results For the control group, contents of TB, ALT, AST and LDH increased significantly and survival time was less than 24 hours. Contents of TB, ALT, AST and LDH of the routine treatment group also increased significantly, but survival time of all the experimental animals was mere than 24 hours. Reduced glutathione and diammonium glycyrrhizinate could reduce the extent of lesion quite significantly 12～24 hours following open abdominal injuries. Conclusions Reduced glutathione and diammonium glycyrrhizinate seemed to have good effects on the treatment and prevention against hepatic damage induced by trauma coupled with seawater immersion. It could be used as a primary means for the treatment of such kind of hepatic damage.     

肝癌细胞分泌物对凝血系统的直接影响

目的：探讨肝癌细胞分泌物对凝血系统的直接影响。方法：以一期法测定肝癌细胞分泌物对APTT、PT、TT及Ⅷ、Ⅴ因子活性的影响。结果：肝癌细胞分泌物使正常血浆APTT缩短、TT延长、Ⅷ因子活性增加、V因子活性降低。异常血浆PT、TT延长。结论：肝癌细胞分泌物可直接改变血浆凝血像。     

LAMS抑制肝癌细胞增殖和促凝活性的实验研究

目的：观察LAMS抑制肝癌细胞增殖和促凝活性的效果。方法：比较LAMS处理的肝癌细胞生长曲线、AgNoRs含量变化;用一步法APTT测定LAMS拮抗肝癌细胞分泌物的促凝血作用。结果：LAMS处理后的肝癌细胞增殖受抑制、AgNoRs数量减少,LAMS能拮抗肝癌细胞分泌物对APTT的影响。结论：LAMS可有效地抑制肝癌细胞增殖和促凝活性。     

昆布多糖硫酸酯对化疗药物治疗肝癌细胞的增敏作用

目的：探讨LAMS对肝癌细胞化疗的增敏作用。方法：以免疫组化法测定用LAMS前后肝癌细胞Bcl—2基因蛋白含量，以MTT法分别测定5—Fu、MTX、MMC、ADM、CTX与LAMS联合及单独治疗肝癌细胞的IC50以及有效作用时间。结果：LAMS使肝癌细胞Bcl—2基因蛋白表达下降，并使肝癌细胞对5—Fu、MTX、MMC、ADM、CTX的敏感性增加，有效作用时间延长。结论：LAMS可有效地增加肝癌细胞对5—Fu、MTX、MMC、ADM、CTX的敏感性。     

供体血管内皮细胞对小鼠-大鼠移植心脏存活时间的影响

目的:探讨供体血管内皮细胞对小鼠-大鼠移植心脏存活时间的影响.方法:分离小鼠血管内皮细胞并于移植前14天经大鼠阴茎背静脉注入2×106细胞/只或联合注射环磷酰胺20mg/Kg·D.异位小鼠-大鼠心脏移植后观察供心存活时间.结果:成功分离小鼠血管内皮细胞,注射小鼠血管内皮细胞14天后,移植心脏平均存活时间10±1.414min,组化染色显示供心有IgM、C3沉积,血浆IgM、IgG、C3、C4较未注射血管内皮细胞组明显增高;联合使用环磷酰胺能延长供心成活时间19.44±2.6034min(t=2.880,P＜0.01).结论:外周静脉注射供体血管内皮细胞不能诱导延迟性异种移植排斥反应(DXR)的免疫耐受,却促使发生HAR.联合环磷酰胺可延长供心存活时间.     

血清碱性蛋白联合紫色反应对肿瘤的筛检价值

目的:用比色及电泳法探索肿瘤生化标志物对常见肿瘤的筛检价值。方法:用改良的血清碱性蛋白电泳(SBPE)及紫色反应检测对照组、炎症组及肿瘤组的血清碱性蛋白(SBP)及肿瘤指数。结果:两项指标对肿瘤组的阳性率为86.7%,特异性77.2%,总有效率83.5%;对常见肿瘤的阳性率分别为:子宫及卵巢癌96.6%,子宫及卵巢肿瘤95.1%,胰腺癌91.2%,肺癌91.2%。肝癌91.1%,直/结肠癌79.4%,胃癌62.5%;子宫及卵巢癌的SBP阳性率90.0%、肿瘤指数83.3%,良性肿瘤时分别为90.2%及31.7%。结论:SBP与肿瘤指数串联是常见肿瘤简单、经济、快速而灵敏的筛查方法,并在子宫及卵巢肿瘤的鉴别诊断中有意义。     

SLIPA喉罩通气瑞芬太尼复合丙泊酚静脉全麻在下肢短小手术中的应用

下肢骨折愈合后内固定取出手术,麻醉要求镇痛完全,对肌松要求不高,全凭静脉麻醉下镇痛药、镇静药都有一定的呼吸抑制作用.麻醉过程中不便于对呼吸的管理,喉罩插管通气技术为呼吸道的建立与管理提供了一种行之有效且简便易行的手段,它具有无需喉镜暴露,放置简便,成功率高,置入时引起心血管反应轻微等优点[1].本研究将SLIPA喉罩通气麻醉用于瑞芬太尼复合丙泊酚静脉全麻应用于下肢短小手术中,并与面罩通气进行比较,观察SLIPA喉罩通气安全性、有效性和可行性.     

胃食管反流病量表(GERD Q)在临床诊治中的应用

目的探讨胃食管反流病量表(GERD Q)在临床诊治中的作用。方法对2009年2月~2013年8月海军总医院消化科436例患者和166例健康体检者进行GERD Q量表评分,同时电子胃镜检查,其中372例行C13尿素呼气试验。结果GERD Q量表的阳性诊断率为64%,GERD患者评分远高于对照组评分(P0.05),治疗前后比较,研究组GERD Q评分明显降低(P0.05),但研究组不同亚型间评分无显著差异(P0.05);幽门螺杆菌HP感染与非感染者GERD Q评分无差别(P0.05)。结论 GERD Q量表虽无益于GERD分型的鉴别,但能够初步诊断GERD,并判断治疗效果,在临床上具有广泛的应用价值。     

IL-6基因多态性与乙型肝炎慢性化的相关性

目的探讨汉族成人IL-6基因多态现象与乙型肝炎慢性化的关系。方法收集118例慢性乙型肝炎(CHB)患者和61例急性乙型肝炎(AHB)患者外周血,提取基因组DNA;采用聚合酶链反应-限制性片断长度多态性(PCR-RFLP)分析方法进行IL-6-572C/G和-597G/A基因分型。结果 CHB组IL-6-572G等位基因频率显著高于AHB组(χ2=8.627,P=0.003)。与CC基因型相比,CG和GG基因型携带者发生慢性化的危险性升高(ORCG=2.024,95%CI:1.009～4.065;ORGG=3.367,95%CI:1.169～9.709)。结论 IL-6-572基因位点多态性与乙型肝炎慢性化有关,携带-572GG基因型的乙型肝炎患者易发生慢性化。