排序方式: 共有19条查询结果,搜索用时 78 毫秒
1.
目的:探讨adipophilin促进细胞内脂质蓄积的可能机制,为动脉粥样硬化的防治提供参考。方法:分别通过q PCR、Western blot和油红O染色观察氧化型低密度脂蛋白(ox LDL)处理RAW264.7细胞不同时间后,细胞内Akt、p-Akt和adipophilin的蛋白水平及脂质蓄积情况;并检测PI3K/Akt抑制剂LY294002处理后,上述指标的变化;HEK293细胞过表达adipophilin后,检测Akt的活性;并用免疫共沉淀实验检测adipophilin与Akt之间的相互作用。结果:ox LDL处理细胞后,随着时间的延长,脂滴增多,Akt被活化,adipophilin表达增加,而LY294002处理则可抑制上述变化;过表达adipophilin后,p-Akt水平增高,但adipophilin与Akt之间未见直接相互作用。结论:Adipophilin可通过PI3K/Akt途径促进细胞内脂质蓄积,但可能不是通过直接相互关系发挥作用的。 相似文献
2.
Objective To investigate the specific humoral immune response and cellular immune response induced by DNA vaccine with Neisseria gonorrhoeae porin B (PorB) fused with B subunit of Escherichia coli heat-labile enterotoxin B (LTB) in mice. Methods Target genes of porB, ltB and ltB-porB were amplified by polymerase chain reaction (PCR) and cloned into eukaryotic vector pcDNA3.1(-). The recombinants were identified by PCR, enzyme digestion and DNA sequencing.The vectors were transfected into Hela cells, and expressed proteins were checked by cytoimmunofluorescence. Female BALB/c mice were intranasally immunized with recombination vectors. The humoral immune response and cellular immune response were detected by enzyme linked immunosorbent assay (ELISA) and methyl thiazolyl tetrazolium (MTT) colorimetric assay. The expressions of recombination vectors in intranasal mucosal tissues of the immunized mice were detected by immunohistochemistry. The means between groups were compared by analysis of variance. Results All the three recombinants were expressed in Hela cells and intranasal mucosal tissues. The PorB specific IgG in serum and sIgA in vaginal secretions in DNA vaccine immunized mice were significantly higher than those in controls (P<0.01 ; P<0.05). Moreover, the sIgA level in pcDNA3.1 (-)/ltB-porB group was higher than that in peDNA3, 1(-)/porB group (P=0. 002). The levels of interferon-gamma (IFN-γ) and interleukin-4 (IL-4) in the supernatants and stimulation index (SI) of spleen lymphocyte culture in pcDNA3, 1(-)/porB group were (170.04±23.89) pg/mL, (114.68±14.27) pg/mL and 1. 68±0.19, respectively; and those in pcDNA3, 1(-)/ltB-porB group were (161.42±27.50) pg/mL, (124.16±19.04) pg/mL and 1.73±0.28, respectively; which were both higher than those in pcDNA3.1(-)/ phosphate buffered saliae (PBS) group (P<0. 01; P<0.05) and pcDNA3.1 (-)/ltB group (all P<0.05), while there was no significant difference between pcDNA3.1 (-)/ltB-porB group and pcDNA3. 1 (-)/porB group (0. 998, 0. 696, 0. 994; all P>0.05). Conclusions The constructed DNA vaccines are all successfully expressed in Hela cells and murine intranasal mucosal tissues. The mucosal immunization of the vaccines [pcDNA3. 1 (- )/porB and pcDNA3.1 ( -)/ltBporB] could induce humoral immune response and cellular immune response, especially mucosal immune response. It is confirmed that mucosal adjuvant LTB could promote PorB to induce higher level of mucosal immune response in mice. 相似文献
3.
目的:初步探讨抗淋病LTB-PorB核酸疫苗与蛋白疫苗联合免疫的免疫增强效应,为研制抗淋病疫苗提供实验依据。方法:大量制备核酸疫苗(pcDNA3.1(-)/ltB-porB)及重组蛋白疫苗(rLTB-PorB);通过鼻饲途径将核酸疫苗和蛋白疫苗采用核酸初免-蛋白加强(DNAprime-protein boost)联合免疫以及分别单独免疫雌性BALB/c小鼠,同时设PBS及空质粒(pcDNA3.1(-))对照组,检测各组体液免疫和细胞免疫应答水平。结果:免疫后第56天,联合免疫组小鼠生殖道sIgAA450(1·083±0·179)、血清IgG A450(1.023±0.116)、脾淋巴细胞诱生的IL-4[(357.58±34.44)/pg/ml]和IFN-γ[(261.85±29.92)/pg/ml]水平均明显高于各对照组(P0.01),小鼠脾淋巴细胞增殖率(SI:2.12±0.29)较对照组显著增高(P0.05);核酸疫苗组、蛋白疫苗组和联合免疫组血清IgG亚类IgG2a/IgG1比值分别为1.678、0.455和0.693。结论:LTB-PorB核酸疫苗和蛋白疫苗联合免疫组诱导的特异性体液免疫应答和细胞免疫应答水平明显优于单独的核酸疫苗组和蛋白疫苗组,且以诱导Th2倾向性免疫应答为主。 相似文献
4.
5.
目的:用真核重组质粒pcDNA3.1(-)/ltB-nspA鼻饲免疫小鼠,探索粘膜佐剂LTB辅佐NspA所诱发的特异性体液免疫应答和细胞免疫应答水平。方法:对真核重组质粒行PCR及双酶切鉴定后大量制备,经鼻饲途径免疫雌性BALB/c小鼠,间接ELISA法检测血清中NspA特异性IgG抗体水平及小鼠生殖道灌洗液中NspA特异性sIgA抗体水平;MTT法检测脾淋巴细胞增殖水平,ELISA双抗体夹心法检测脾淋巴细胞培养上清IFN-γ含量。结果:融合基因pcDNA3.1(-)/ltB-nspA组小鼠生殖道灌洗液中sIgA(A450:0.316±0.045)明显高于pcDNA3.1(-)/nspA单基因组(P0.05)和其它对照组(P0.01);小鼠血清中IgG(A450:0.643±0.156)水平、脾淋巴细胞刺激指数(SI:1.65±0.32)和脾淋巴细胞诱生的IFN-γ(160.56±25.67pg/ml)水平均明显高于pcDNA3.1(-)/ltB、空质粒pcDNA3.1(-)和PBS对照组(P0.01)。结论:pcDNA3.1(-)/ltB-nspA融合基因疫苗经鼻饲免疫,能够诱导小鼠产生较强的特异性体液免疫应答和细胞免疫应答;粘膜佐剂LTB可辅佐NspA诱导小鼠产生更高水平的生殖道粘膜免疫。 相似文献
6.
目的 分析影响皮肤恶性黑色素瘤(CMM)预后的因素,为临床治疗及预后预测提供依据。
方法 回顾性分析2009 年1 月-2015 年12 月在新疆医科大学第三临床医学院治疗的153 例CMM 患者的临
床资料及随访情况。以患者性别、年龄、肿瘤溃疡、肿瘤部位、肿瘤厚度、肿瘤分期、BRAF V 600E 突变、
乳酸脱氢酶(LDH)、中性粒细胞与淋巴细胞比值(NLR)、放疗、干扰素治疗为影响因素。分析上述指标对
患者预后的影响,并进行生存分析。结果 单因素分析结果显示,肿瘤溃疡、肿瘤部位、肿瘤厚度、肿瘤分期、
LDH、BRAF V 600E 突变、NLR 为影响CMM 患者预后的因素(P <0.05)。多因素分析结果显示,肿瘤溃疡、
肿瘤厚度、肿瘤分期、NLR 为影响CMM 患者生存时间的独立预后危险因素(P <0.05)。依据独立预后危险
因素综合分析患者的预后,各危险组生存时间比较,差异有统计学意义(P <0.05)。结论 肿瘤溃疡、肿瘤厚
度、肿瘤分期、NLR 为影响CMM 患者预后的独立危险因素。 相似文献
7.
8.
目的比较研究锁骨钩钢板与Endobutton袢钢板治疗肩锁关节脱位。方法 60例肩锁关节脱位的患者,38例采用锁骨钩钢板内固定,22例采用Endobutton袢钢板内固定。回顾性分析比较手术时间、术中出血量、术后并发症、术后肩关节功能评分。结果术后平均随访18个月,锁骨钩钢板在手术时间、术中出血量方面优于Endobutton袢钢板,但是术后并发症多于Endobutton袢钢板。肩关节功能评定,Endobutton袢钢板优良率97.3%,锁骨钩钢板组77.4%。结论 Endobutton袢钢板与锁骨钩钢板在治疗上总体相当,术后并发症少于锁骨钩钢板,术后肩关节功能显示有明显优势。 相似文献
9.
10.
目的比较附加伤椎的六钉经椎弓根螺钉内固定与跨伤椎的四钉经椎弓根螺钉内固定治疗胸腰椎骨折的效果。方法2008年11月至2011年9月,选择50例胸腰椎骨折患者随机给予附加伤椎的六钉经椎弓根螺钉内固定手术(18例,六钉组)和跨越伤椎的四钉经椎弓根螺钉内固定手术(32例,四钉组),对两组患者围手术期相关指标、后凸畸形矫正情况、术后神经功能恢复、腰背痛改善等进行比较。结果六钉组与四钉组相比。手术切口长度、手术时问、术中出血量、术后引流量、输血量、术后住院时间、术后使用止痛药的比例,以及术后手术伤口疼痛VAS评分、术后并发症等均无显著统计学差异(P〉0.05)。两组术前与术后影像学比较,矢状位后凸Cobb角、伤椎自身成角、伤椎椎体前缘高度均有显著性差异(P〈0.01)。六钉组术后Cobb’s角、伤椎自身成角、伤椎前缘高度的改善程度与四钉组比较差异无统计学意义(P〉0.05),随访期间两组内固定失败率(椎弓根螺钉松动、断裂)、术后矫正度丢失、术后神经功能恢复、腰背痛改善差异无统计学意义(P〉0.05)。结论附加伤椎的六钉经椎弓根螺钉内固定与跨越伤椎的四钉经椎弓根螺钉内固定治疗胸腰椎骨折的临床效果相当。 相似文献