电灼结合中药内服外洗治疗尖锐湿疣的疗效观察

目的观察电灼结合中药内服外洗治疗尖锐湿疣的临床疗效及对患者T淋巴细胞亚群的影响。方法选择从2013年7月至2017年11月山西医科大学附属大同市第三人民医院泌尿外科治疗尖锐湿疣的患者148例,随机分成对照组和观察组两组,每组74例。对照组患者单独采用电灼治疗;观察组患者在采用电灼基础上结合中药内服外洗共同治疗。结果观察组尖锐湿疣患者复发率为10.8%,低于对照组尖锐湿疣患者复发率28.4%,差异具有统计学意义。(P<0.01)。两组治疗后复发组CD3+细胞、CD4+细胞水平、CD4+/CD8+细胞比值较未复发组低,而CD8+细胞水平较未复发组高,差异具有统计学意义。(P<0.05)。治疗后,观察组尖锐湿疣患者CD3+水平、CD4+水平、CD4+/CD8+比值高于对照组尖锐湿疣患者,且观察组尖锐湿疣患者CD8+水平低于对照组尖锐湿疣患者CD8+水平,差异具有统计学意义(P<0.01)。结论电灼结合中药内服外洗能减少尖锐湿疣的复发率。在临床治疗中,监测尖锐湿疣患者外周血T淋巴细胞亚群,对尖锐湿疣患者治疗后的复发预估及相应增强免疫治疗具有重要的指导意义。     

Changes in T .lymphocyte subsets after severe traumatic brain inJury

BACKGROUND： Besides local changes of cranial parenchymal cells, hemorrhage, etc., severe traumatic brain injuries also cause the changes of total body fluid and various functions, and the changes of lymphocytes and T lymphocyte subsets should be paid more attention to. OBJECTIVE： To reveal the changing laws of T lymphocyte subsets after severe traumatic brain injury, and compare with mild to moderate brain injury. DESIGN： A comparative observation. SETTINGS： Department of Neurosurgery, Longgang District Buji People＇s Hospital of Shenzhen City; Central Laboratory of Shenzhen Hospital of Prevention and Cure for Chronic Disease. PARTICIPANTS： All the subjects were selected from the Department of Neurosurgery, Longgang District Buji People＇s Hospital of Shenzhen City from August 2002 to August 2005. Thirty patients with severe brain injury, whose Glasgow coma score （GCS） was ≤ 8 points, were taken as the experimental group, including 21 males and 9 females, aging 16 - 62 years. Meanwhile, 30 patients with mild traumatic brain injury were taken as the control group （GCS ranged 14- 15 points）, including 18 males and 12 females, aging 15 -58 years. All the subjects were in admission at 6 hours after injury, without disease of major organs before injury Informed consents were obtained from all the patients or their relatives. METHODS： （1） The T lymphocytes and the subsets in peripheral blood were detected with immunofluorescent tricolor flow cytometry at l, 3, 7 and 14 days after injury in both groups. （2） The conditions of pulmonary infections were observed at 4 days after injury. The differences of measurement data were compared with the t test. MAIN OUTCOME MEASURES： Changes of T lymphocytes subsets at 1 - 14 days after severe and mild or moderate traumatic injury. RESULTS： Finally, 28 and 25 patients with mild to moderate traumatic brain injury, whereas 25 and 21 patients with severe traumatic brain injury were analyzed at 7 and 14 days respectively, and the missed ones died due to the development of disease. （1） Changes of T lymphocyte subsets： At 1 and 3 days after injury, CD3, CD4, CD8, CD4/CD8 began to decrease, whereas CD8 increased in the experimental group, which were very significantly different from those in the control group （t =2.77 - 3.26, P 〈 0.01）, and began to recover at 7 days, which were significantly different from those in the control group （t = 2.06 - 2.24, P 〈 0.05）, and generally recovered to the normal levels at 14 days （P 〉 0.05）. （2） Conditions of pulmonary infections： At 4 days after injury, the rate of pulmonary infection was significantly different between the experimental group and control group [73% （22/30）, 0, x2=37.29, P 〈 0.01]. CONCLUSION： Patients with severe traumatic brain injury suffer from damages of cellular immune function at early period （within 7 days）, and they are easily to be accompanied by pulmonary infections.     

胸腺肽α1对老年晚期恶性肿瘤患者细胞免疫功能的影响

目的 :观察胸腺肽α1对老年恶性肿瘤患者细胞免疫功能的影响。方法 :30例老年恶性肿瘤患者 ,用胸腺肽α1治疗 2个月 ,第 1个月每日 1.6 mg,第 2个月隔日 1.6 mg,均为皮下注射 ,观察治疗前后 T细胞亚群及 NK细胞、患者生活质量、血常规及肝肾功能变化。结果 :30例患者用胸腺肽α1前 ,CD4、CD4 /CD8、NK细胞分别为 (32 .33± 6 .2 5 ) %、(0 .77± 0 .2 3) %、(16 .0 5± 6 .79) % ,治疗后为 (39.4 2± 9.2 6 ) %、(1.19± 0 .5 3) %、(2 4 .37± 8.2 3) %。治疗前后比较均有显著意义 (P<0 .0 5 ) ,患者生活质量分值治疗后比治疗前显著提高 (P<0 .0 5 )。结论 :胸腺肽α1能提高老年晚期恶性肿瘤患者的细胞免疫功能 ,是较理想的免疫增强剂。     

急性高容量性血液稀释对妇科肿瘤患者围术期T淋巴细胞亚群的影响

目的探讨急性高容量性血液稀释(AHH)对妇科肿瘤患者围手术期T淋巴细胞亚群的影响.方法40例ASAⅠ～Ⅱ级的妇科肿瘤手术病人随机分成两组:高容量性血液稀释组(A组)在切皮前30分钟内输入6%羟乙基淀粉液1000ml;对照组(C组)常规输液,不实施血液稀释.分别与麻醉诱导前、手术结束后、术后24小时抽取静脉血,用流式细胞仪测定T淋巴细胞亚群CD3 、CD4 、CD8 及CD4 /CD8 细胞百分率.结果两组病人手术结束后(T1)、手术后第1天(T2)与麻醉诱导前(T0)比较:T淋巴细胞亚群CD3 、CD4 及CD4 CD8 均明显降低(P＜0.01或P＜0.05),CD8 无显著性变化;但A组手术结束后和手术后第1天CD3 、CD4 及CD4 /CD8 明显高于C组(P＜0.05).结论术前采用急性高容量性血液稀释(AHH),可显著改善妇科肿瘤患者T淋巴细胞的免疫功能.     

A Comparative Study on the Effect of BCG-PSN and Thymopeptides on T-lymphocyte Subsets of Normal and Immunosuppressed Mice

Polysaccharidenucleicacidfractionofbacilluscalmetteguerin (BCG PSN ,SiqikangInjection)andthymopeptidesarenowtwowidelyusedimmunomod ulatorsinclinicalpractice .Theyareusuallyusedasanadjuvanttherapyforvirusinfection ,autoimmunediseasesandneoplasms ,whichhavebeenclinicallyprovedtobeeffective .Somereportsdemonstratedthattheybothcanstimulatetheproliferationanddif ferentiationofT lymphocytes.However ,theexactmechanismshavenotbeenelucidatedyet .InordertocomparetheirmodulatingmechanismsonT lympho c…     

分次照射对兔外周血T淋巴细胞DNA合成影响动态观察

本文对长耳白兔受一次与分次~(60)Cor线不同剂量照射后一个月内其外周血T淋巴细胞DNA合成率进行了连续观察。结果表明一次性照射各剂量组均在照后第1天DNA合成受抑最严重;照后第3天各组开始恢复;第7天各组均恢复到照前水平。在分次照射停照后1.0Gy组不但未下降反而有升高趋势。当剂量率、累积剂量均相同,仅分割次数不同时发现0.05Gy/d组受抑程度比0.10Gy/d组明显。     

高原地区健康老年人重返平原后外周血T细胞亚群变化的观察

用单克隆抗体测定了移居高原的老年人重返平原后的T细胞亚群的变化结果:①移居西宁(2260m)组、天峻(3000m)组在西宁所测的OKT_3、OKT_4、OKT_8及OKT_4/OKT_8值与在苏州所测的当地老年人无差异。②移居西宁组急返平原后OKT_3、OKT_8水平明显低于返回平原后居住一年以上者(以下简称返回组)(P<0.01～0.001)但OKT_4/OKT_8无差异。③返回组与世居苏州老年人相比,前者OKT_3、OKT_4、OKT_8及OKT_4。OKT_8增高,其中OKT_3、OKT_4增高明显(P<0.01～0.02),作者认为长期移居高原返回平原后T细胞亚群也可能存在一“脱适应”阶段,即机体重新调整重新平衡的一种形式,这一阶段可能需一年以上。     

分泌IL－5T细胞杂交瘤的培养及IL－5的分离纯化

高浓度ＩＬ－５可以从Ｔ细胞杂交瘤培养的上清液中获得。本文介绍分泌ＩＬ－５的Ｔ细胞杂交瘤的培养及其上清液中分高纯化ＩＬ－５的方法，提纯了高浓度的ＩＬ－５。     

胸腺肽α1对神经外科肿瘤患者T淋巴细胞亚群和NK细胞的影响

目的了解胸腺肽α1对神经外科肿瘤患者围术期T淋巴细胞亚群和自然杀伤(NK)细胞数量的影响。方法选择30例神经外科肿瘤手术的患者,随机均分为研究组和对照组,研究组麻醉前用胸腺肽α1,对照组未使用。分别于麻醉前、手术1 h、术毕和术后5 d抽取外周静脉血,用流式细胞仪检测T淋巴细胞亚群(CD3 、CD4 、CD8 )和NK细胞(CD56 )数量的变化。结果研究组和对照组手术1 h CD3 、CD4 、CD4 /CD8 、NK细胞数量均较麻醉前明显下降(P<0.05或P<0.01),研究组下降幅度较对照组小(P<0.05),术毕研究组和对照组各指标有所回升,但研究组回升的幅度较对照组大,术后5 d研究组和对照组各指标均回到麻醉前水平。结论胸腺肽α1减小了神经外科肿瘤患者围术期T淋巴细胞亚群和NK细胞下降的程度,能明显减轻细胞免疫功能的抑制。     

TRAb和T淋巴细胞亚群联合检测对预测Graves病复发的临床意义

采用酶联免疫法和单克隆抗体法测定４２例Ｇｒａｖｅｓ病人，在用丙基硫氧嘧啶（ＰＴＵ）治疗过程中ＴＳＨ受体抗体（ＴＲＡｂ）和外周血Ｔ淋巴细胞亚群的变化，并对病人停药后随诊一年的结果表明ＰＴＵ治疗１８个月后，ＴＲＡｂ持续高水平和Ｔ淋巴细胞未恢复到正常者易复发。