蓬辣滩水电站泄洪闸门及其监控系统改造

介绍了蓬辣滩水电站泄洪闸门的状况、计算机监控系统改造方案的结构和特点及闸门定轮轴承的改造情况,通过上述改造,提高了泄洪设备运行的自动化水平,同时也为其他闸门工程提供参考。     

17β-雌二醇对子宫内膜异位症患者在位子宫内膜间质细胞β-catenin mRNA和蛋白表达的影响

目的研究17β-雌二醇(17β-E2)对子宫内膜异位症(内异症)患者在位子宫内膜间质细胞β-catenin mRNA和蛋白表达的影响,探讨Wnt/β-catenin信号通路在介导雌激素促进内异症发生发展的作用。方法体外分离培养内异症患者在位子宫内膜间质细胞。用不同浓度17β-E2处理子宫内膜间质细胞48 h;此后选用10-10mol/L 17β-E2处理子宫内膜间质细胞12、24和48 h,逆转录聚合酶链反应(RT-PCR)和免疫印迹法(Western blotting)检测17β-E2处理前后子宫内膜间质细胞β-catenin mRNA和蛋白的表达水平。同法分析雌激素受体拮抗剂ICI182,780(10-6mol/L)对17β-E2促进β-catenin mRNA和蛋白表达的影响。免疫组织化学染色观察17β-E2作用后β-catenin在子宫内膜间质细胞中的定位。结果17β-E2能明显促进内异症患者在位子宫内膜间质细胞β-catenin mRNA和蛋白的表达,并呈剂量和时间依赖性,于10-10mol/L作用48 h最明显。雌激素受体拮抗剂ICI182,780能明显抑制17β-E2对子宫内膜间质细胞β-catenin mRNA和蛋白的表达。免疫组织化学染色发现17β-E2能促进β-catenin在子宫内膜间质细胞核内的表达。结论雌激素可能通过激活Wnt/β-catenin信号通路促进内异症在位子宫内膜的异位种植。     

唐红运用中药内外合治糖尿病周围神经病变经验

介绍唐红主任医师治疗糖尿病周围神经病变的临床经验,主张运用中药内外合治,并自创活血化瘀之糖络宁外敷穴位。     

Monoclonal antibody identification of mononuclear cells in endomyocardial biopsy specimens from a patient with rheumatic carditis

A 17-year-old woman with rheumatic carditis underwent endomyocardial biopsy both prior to and following treatment with prednisone and aspirin. Frozen sections from the endomyocardial biopsy specimens were studied with monoclonal antibodies by an indirect immunofluorescence technique to define the composition of the inflammatory infiltrate in the myocardium and to determine whether the composition of the infiltrate is distinctive and diagnostically useful. The specimen from the initial biopsy contained a heterogeneous infiltrate composed of T lymphocytes, macrophages, B lymphocytes, and mast cells. T lymphocytes predominated, and the ratio of T-helper to T-cytotoxic/suppressor cells was 2.0. Following treatment the overall cellularity of the infiltrate was diminished, but the infiltrate remained heterogeneous; T cells predominated, and the T-helper to T-cytotoxic/suppressor ratio was reversed, to 0.59. The composition of the inflammatory infiltrate in this case of rheumatic carditis distinguishes it immunologically from other "idiopathic," presumably virus-associated, forms of myocarditis.     

Relationship between corneal thickness and measured intraocular pressure in a general ophthalmology clinic

OBJECTIVE: To assess whether central corneal thickness (CCT) is a confounding factor in the classification of patients attending for glaucoma assessment in a district general hospital. DESIGN: Cross-sectional study by a single observer. PARTICIPANTS: Patients attending a general ophthalmic clinic: 235 clinically normal eyes, 52 eyes with normal-tension glaucoma (NTG), 335 eyes with primary open-angle glaucoma (POAG), 12 eyes with pseudoexfoliative glaucoma (PXE), 42 eyes with chronic angle closure glaucoma (CACG), and 232 glaucoma suspect (GS) eyes. INTERVENTION: Central corneal thickness was measured using ultrasonic pachymetry. MAIN OUTCOME MEASURE: Correlation of CCT and diagnosis. RESULTS: Mean CCT was 553.9 microm (95% confidence intervals [CI] for the mean, 549.0-558.8 microm) in the clinically normal eyes, 550.1 microm (95% CI, 546.6-553.7 microm) in the POAG eyes, 514.0 microm (95% CI, 504.8-523.3 microm) in the NTG eyes, 530.7 microm (95% CI, 511.2-550.1 microm) in the PXE eyes, 559.9 microm (95% CI, 546.8-573.0 microm) in the CACG eyes, and 579.5 microm (95% CI, 574.8-584.1 microm) in the GS eyes. The differences of mean CCT between the groups were highly significant (P< 0.001 analysis of variance). Eighty-five percent of eyes with NTG and only 36% of eyes with POAG had a mean CCT of 540 microm or less. Thirteen percent of eyes with POAG and 42% of GS eyes had a mean CCT greater than 585 microm. CONCLUSIONS: The CCT measurement is desirable in patients attending for glaucoma assessment in a district general hospital to avoid misclassification resulting from the relationship between CCT and tonometric pressure. Central corneal thickness alone is not an accurate predictor for the clinical diagnosis in this group of eyes. However, many eyes diagnosed as having NTG have thin corneas, which would tend to lower the tonometrically recorded intraocular pressure (IOP), so the finding of a less-than-normal thickness cornea introduces some doubt as to the diagnosis of NTG. For the GS eyes, most eyes had thick corneas, which would tend to increase the tonometrically recorded IOP. Thus, GS eyes with modest elevation of IOP and a thick cornea may be at low risk of progressing to POAG. Thus, many patients with "high IOPs" and a thick CCT do not necessarily have high IOPs and may not need to be followed as GS eyes.     

Focal segmental membranous glomerulonephropathy associated with other glomerular diseases

In four patients with the nephrotic syndrome, renal biopsy revealed focal segmental membranous glomerulonephropathy (FSMGN) associated with the histologic patterns of "nil" disease (two cases), hereditary nephritis and diffuse diabetic glomerulosclerosis. The occurrence of FSMGN in association with other glomerular diseases, presumably unrelated to immune complex deposition, is infrequent in our experience. Rather than necessarily representing an early stage or milder form of membranous glomerulonephropathy, it may be an epiphenomenon. This interpretation has prognostic and therapeutic implications and raises important pathogenetic questions. In particular, this study suggests that in some instances, preexisting functional and structural abnormalities may play a role either in the deposition of preformed circulating immune complexes or in the local formation of immune complexes.     

重组人Ⅱ型胶原250-270多肽的表达、纯化和鉴定

目的:表达与纯化重组人Ⅱ型胶原250-270多肽(rhCⅡ250-270),鉴定并确认rhCⅡ250-270多肽与预期的相符。方法:在E.coli BL 21中表达rhCⅡ250-270多肽,使用亲和层析法分离纯化。用限制性酶切、聚合酶链式反应(PCR)、基因序列测定、琼脂糖凝胶电泳、聚丙烯酰胺凝胶电泳(SDS-PAGE)和免疫印迹等方法在基因和蛋白水平上对rhCⅡ250-270多肽进行鉴定。结果:纯化的rhCⅡ250-270多肽,大小约43 kD,纯度为89.3%。E coRⅠ和SalⅠ双酶切法和PCR法鉴定测得DNA大小约为500bp和650bp,核苷酸序列测定证实基因DNA序列与设计的完全相符。SDS-PAGE显示rhCⅡ250-270多肽目的蛋白条带的大小约为43 kD,W estern B lotting证实GST融合蛋白上存在CⅡ片段。结论:表达和纯化rhCⅡ250-270多肽与预期的相符,并发现此多肽具有较强的免疫原性。     

糖尿病夏科足临床特点分析

目的总结糖尿病夏科足的临床特点,提高临床医师对于本病的认识。方法回顾性分析11例2型糖尿病夏科足患者的临床特点。结果 11例患者中,男7例,女4例;平均年龄(60±11)岁;平均糖尿病病程16年;全部合并神经病变而无下肢血管病变;单侧足病变10例,双足病变1例;3例发病前有明确的外伤史。影像学表现为足部骨质破坏7例,关节间隙变窄6例,软组织肿胀3例,关节半脱位2例,关节融合2例,碎骨1例。11例患者一入院均遵医嘱限制活动。对足溃疡给予积极清创,换药。根据细菌培养及药敏试验结果选用抗生素治疗。10例合并足溃疡病例中,1例截趾后愈合,3例愈合,5例好转,1例自动出院后失访。3例出院后有足溃疡的复发。结论早期识别并给予制动等措施有助于足溃疡愈合和减缓夏科足畸形的发展。     

西安市道路清扫保洁成本费用分析

通过对西安市城六区道路人工和机械清扫保洁实际成本费用和优缺点的对比分析,建议采用效率最大、成本最佳的道路清扫保洁作业形式,即在确保清扫保洁质量的前提下,西安市应大力发展机械清扫、人工保洁的模式。