基于实验均方误差F值的基因表达数据最佳聚类评判方法研究

目的 近年来产生了一些用于分析基因表达数据的聚类算法，却很少有关于评价聚类算法方法的研究。本研究的目的是尝试建立一个定量的评价基因表达数据聚类结果的方法。方法 本研究提供了一个系统的评价聚类结果的方法，利用我们提出的实验均方误差F值对几个常见的聚类算法进行比较。结果 利用F值对类质量的评价和利用已有的生物学知识对类进行分析的结果一致。结论 实验均方误差F值可以定量地评判用于基因表达数据的聚类算法。     

视觉电生理联合应用对眼外伤视觉功能的评价

联合使用视觉电生理检查法评估外伤眼的视功能。方法单纯眼外伤３０例按受伤程度分３组,健康对侧眼为对照组,比较各组ＶＥＰＰ１００波潜伏值,３０Ｈｚ红闪炮光ＥＲＧ的振幅和暗适应ＥＲＧａ、ｂ波振幅的平均值。结论联合应用视觉电生理检测法是评价眼外伤视功能的可靠依据。     

The influence of body size on the pharmacodynamic and pharmacokinetic response to clopidogrel and prasugrel: A retrospective analysis of the FEATHER study

Introduction

Patients treated with clopidogrel who have higher body size exhibit greater platelet reactivity than patients with lower body size. In a retrospective analysis of the FEATHER trial, we examined the relationship between platelet response to thienopyridines clopidogrel 75 mg (Clop-75), prasugrel 5 mg (Pras-5), and prasugrel 10 mg (Pras-10) using 3 body size indices: body weight (BW), body mass index (BMI), and body surface area (BSA). Relationships were assessed as continuous variables and as 4 incremental body size groups.

Materials and Methods

Aspirin-treated patients with stable coronary artery disease (N = 72) and a BW range of 45-134 kg received Clop-75, Pras-5, and Pras-10 in a 3-period, blinded, cross-over study. Platelet assays included maximum platelet aggregation (MPA) to 20 μM ADP by light transmission aggregometry, VerifyNow-P2Y12 reaction units (PRU), and vasodilator-associated stimulated phosphoprotein (VASP) phosphorylation platelet reactivity index (PRI). Exposure to active metabolites (AMs) was also assessed.

Results

Body size was a determinant of AM exposure and residual platelet reactivity regardless of type and dose of thienopyridine. BW and BSA demonstrated marginally stronger correlations with platelet reactivity; VASP-PRI demonstrated a stronger correlation with the body size than the other tests. Correlation coefficients ranged from a high of 0.64 (BW vs. PRI on Pras-5) to a low of 0.34 (BMI vs. MPA on Pras-10), but all were statistically significant (p < 0.01).

Conclusions

Using a comprehensive selection of body size indices, AM exposures, platelet function tests, and thienopyridine doses, we demonstrated a consistent inverse relationship between body size and response to clopidogrel and prasugrel.     

加强医院医疗设备资产管理的若干思考

本文从强化和完善大型综合性医院资产管理的角度出发,剖析目前大型综合性医院资产管理存在的问题,并提出相应的对策和建议。     

脑卒中患者日常生活活动能力评定

日常生活活动能力的评估是脑卒中患者康复治疗最重要的评估内容之一,它包括了人们为独立生活而每天必须进行的基本动作。文章介绍了脑卒中患者日常生活能力评估的最常见量表和评定方法以及评定注意事项。     

地震后医护人员心理卫生状况的调查分析

目的调查地震灾害一年后在都江堰市工作的医护人员心理卫生状况和相关因素,为以后开展心理咨询和治疗提供科学依据。方法采用症状自评量表（SCL-90）,对灾区都江堰市8家医院的220名医护人员（调查组）和非受灾区崇州市7家医院的213名医护人员（对照组）进行心理健康评定比较并同时与全国常模比较,对结果进行分析。结果SCL-90显示,都江堰市8家医院的220名医护人员除人际关系敏感外,其他相关因子分数均高于全国常模,P〈0．05。结论地震一年后,都江堰市基层工作的医护人员依然存在心理健康问题,心理健康情况总体低于全国常模水平。在帮助受灾病人的同时,他们也受到地震冲击,应给予他们心理支持,使他们尽早走出心理阴影。     

孕妇配偶的压力与性格特征的相关性

目的:描述孕妇配偶的压力水平及性格特征,探讨压力与性格特征的相关性。方法:对167例孕妇配偶用妊娠压力量表测量其压力水平,用艾森克个性问卷测量其性格特征,并分析性格特征对压力的影响。结果:压力量表测量赤示孕妇配偶的压力处于中等水平(M=1.53);艾森克个性问卷示孕妇配偶E因子(性格内外向)、P因子(精神病质)、N因子(神经质)及L因子(掩饰程度)均高于常模,具有性格内向,有精神病质和神经质双重倾向,与常模(n=1000)比较,差异有显著性意义(t=22.32～34.88,P均>0.05);孕妇配偶的压力水平与精神病质、性格内外向及神经质得分成正相关(r=0.196～0.347,P<0.05)。结论:孕妇配偶存在中等程度的压力,其不良性格特征会增加压力,提示临床护理工作者可以用艾森克个性问卷对孕妇配偶进行心理测试,对于有不良性格特征的孕妇配偶应给予重点关注。     

临床课程教学质量评价分析

通过对2002-2005学年临床理论课程质量评价结果列表和统计学分析讨论，总结提高教学质量的方法和成效，改进教学质量评估体系，培养高素质、实用型的医学人才。     

用评估系统评价测评工具对国内外五篇静脉鱼油对结局影响的Meta分析的质量评价

目的　用评估系统评价测评工具（AMSTAR）对静脉鱼油对手术后及重症监护患者影响的系统评价进行质量评价。方法　计算机检索1996年1月至2013年9月美国生物医学文献检索系统（PubMed）、荷兰医学文摘检索系统（EMBASE）、Cochrane图书馆、Web of Science、中国生物医学文献数据库、中国知网、万方数据平台等数据库中研究静脉鱼油对患者临床结局影响的系统评价及Meta分析文献,同时手工检索临床营养相关期刊中论文,并回溯相关论文的参考文献。由两名研究者按照纳入标准独立筛选文献、提取资料后,采用AMSTAR量表评价纳入的系统评价质量。结果　共纳入5篇相关Meta分析。基于AMSTAR的质量评价结果显示,2篇Meta分析为高质量,1篇为中等质量,2篇为低质量。结论　已发表静脉鱼油对手术后及重症监护患者影响的Meta分析质量参差不齐,有部分为低质量Meta分析。今后需要更多样本量较大、质量较高的随机、对照、双盲研究报道来更新系统评价,观察静脉鱼油对手术后及重症监护患者的临床结局和成本-效果影响,以期得出更可靠的结论。     

Acupuncture treatment of glaucoma based on radar plots: A protocol for an overview of systematic reviews

Background:Glaucoma is the second most blinding eye disease in the world. Currently, lowering the intraocular pressure through various methods is the main treatment of glaucoma. Acupuncture has been effectively and safely used in the treatment of glaucoma. However, the evidence for the efficacy of acupuncture in the treatment of glaucoma is controversial, leading to inconsistent findings from systematic evaluations at abroad and home. Therefore, this protocol aims to provide a multivariate evaluation on the quality of evidences from current systematic reviews (SRs) and/or meta-analyzes (MAs) of acupuncture in the treatment of glaucoma, and literature quality, thus providing an intuitive and reliable evidence synthesis and basis for clinical decision making.Methods:MAs/SRs about the acupuncture treatment of glaucoma will be searched online, including Chinese Biomedical Literature Database (CBM), China Science and Technology Journal Database (VIP), China National Knowledge Infrastructure (CNKI), WanFang Database (WF), Web of Science, Embase, PubMed, and Cochrane Library. Two investigators will independently screen literatures according to inclusion and exclusion criteria and extract data. A multivariate evaluation of the included literature will be performed by depicting radar plots in 6 aspects as follows: Year of publication, study type, SRs assessment through the Assessment of Multiple Systematic Reviews 2 (AMSTAR 2), literature quality assessment through the Preferred Reporting Items for Systematic Reviews and Meta-analyses (PRISMA), homogeneity, and publication bias. The Grading of Recommendations Assessment, Development, and Evaluation evidence quality assessment tool will be used to grade and evaluate the quality of outcome indicators of the included literatures.Results:This study will be submitted for publication in a peer-reviewed journal.Conclusion:We would like to provide a visual and scientific approach for clinical decision making of acupuncture treatment of glaucoma through a accessible and useful assessment of systematic reviews.