股骨头缺血性坏死的介入治疗

通过超选择插管至旋股内外侧动脉及闭孔动脉髋臼支,将扩血管药物、溶栓药物直接灌注到股骨头颈区血管内,改善股骨头血液循环,溶通和重建股骨头滋养血管,对其临床疗效进行观察.     

胆道引流术后荧光腹腔镜手术治疗胆囊结石的临床疗效

背景与目的:腹腔镜胆囊切除术(LC)中经胆道直接注射吲哚菁绿(ICG)行荧光胆管显影,有即刻显影及零肝脏背景荧光的优势,在复杂LC中有更好的效果.本研究旨在探讨经皮经肝胆囊穿刺引流术(PTGBD)或经内镜鼻胆管引流术(ENBD)术后行荧光LC的临床效果.方法:选取2019年1月-2021年4月120例行LC的胆囊结石患...     

复方鱼腥草对鲍曼不动杆菌体外培养生长的影响

目的:探讨复方鱼腥草是否能抑制鲍曼不动杆菌生长,为临床上治疗鲍曼不动杆菌感染提供潜在新药。方法:根据临床药敏结果选取20株鲍曼不动杆菌菌株和标准菌株ATCC19606作为实验对象,用微量肉汤稀释法和琼脂稀释法检测鲍曼不动杆菌在含不同浓度复方鱼腥草培养基中的生长情况,肉眼判断最低抑菌浓度(minimum inhibitory concentration,MIC)。结果:不同浓度的复方鱼腥草能影响鲍曼不动杆菌生长,随药物浓度的增加,抑菌活性增强,最低抑菌浓度分别为3200⁶ 400μg/m L(微量肉汤法),8006 400μg/m L(微量肉汤法),800⁶ 400μg/m L(琼脂稀释法)。结论:复方鱼腥草对鲍曼不动杆菌体外生长具有抑制作用,可能作为潜在药物用于抗鲍曼不动杆菌感染。     

单侧隐睾大鼠对侧睾丸组织中SCF/c-kit基因表达变化及意义

Objective To investigate the pathologic changes and expressions of SCF and c-kit in the contralateral testes in rat model of unilateral cryptorchidism. Methods Thirty male SD rats were maintained under controlled temperature and constant photoperiodic conditions with access to food and water. The rats were randomly assaigned to the control group and the experimental unilateral cryptorchidism group. The left testis of the rats in the unilateral cryptorchidism group was placed into the abdominal cavity. The control group rats were subjected to sham surgery. Three months later, the rats were sacrificed and their right testes were harvested. The pathological changes were observed under microscope. The mRNA and protein expression of SCF and c-kit were also investigated using quantitative Real-time RT-PCR, Western blotting and immunohistochemical staining. Apoptotic germ cells were detected by TUNEL staining. Results All rats survived to the endpoints. The right testes of rats of the unilateral cryptorichid group were smaller than those of the control group. Normal testes of the control group rats manifested active spermatogenesis and orderly arrangement of germ cells.However, disordered and sloughed germinal cells with less distinct seminiferous tubule borders were observed in the contralateral testes of rats with experimental unilateral cryptorichid under the microscope. Cmpared with the control testes, the mRNA and protein expression of SCF and c-kit of the contralateral testes of rats with experimental unilateral cryptorichid was decreased (P＜0.05). The apoptotic germ cells were increased (19.7±3.83 vs 5. 4 ± 1.02, P＜0. 05). The SCF/c-kit expression was positively correlated with the germ cell apoptosis (P＜0. 01 ). Conclusions The decreased expression of SCF and c-kit and increased germ cell apoptosis are found in the contralateral testes, which may contribute to thc infertility in the rat model of experimental unilateral cryptorchidism.     

SALL2基因在口腔肿瘤中的表达和意义

目的 研究婆罗双树样基因2(SALL2)在口腔肿瘤中的表达,探究其对口腔肿瘤细胞增殖、迁移能力的影响.方法 构建SALL2基因过表达及敲低的口腔肿瘤(SCC-3)细胞株,通过MTT实验、细胞划痕实验观察细胞增殖、迁移的变化;收集15组口腔肿瘤组织与瘤旁组织,应用QRT-PCR法检测组织中SALL2 mRNA的表达.结果 细胞划痕试验与MTT实验结果表明过表达SALL2基因可降低细胞增殖、迁移能力,敲低SALL2基因可增强细胞增殖、迁移能力;SALL2基因在口腔肿瘤中的表达明显低于正常口腔组织(P<0.05).结论 SALL2基因在口腔肿瘤细胞及组织中低表达,可抑制肿瘤细胞的增殖、迁移,可能作为口腔肿瘤的早期诊断和预后判断的早期指标.     

生长抑素联合乌司他丁治疗重症胰腺炎的临床效果观察

目的 探究生长抑素联合乌司他丁治疗重症急性胰腺炎的临床效果.方法 将82例重症胰腺炎患者随机分为对照组和观察组,每组41例.对照组予以常规治疗+生长抑素治疗,观察组在对照组的基础上加用乌司他丁治疗,比较两组治疗效果.结果 治疗14 d后,两组患者临床症状均有改善,观察组治疗有效率(92.7%)明显高于对照组(78.0%),差异有统计学意义(P<0.05).患者白细胞计数、血淀粉酶水平、C反应蛋白治疗后明显改善,观察组效果更优,且并发症发生率显著低于对照组,差异均有统计学意义(P<0.05).结论 生长抑素和乌司他丁联合使用可明显提高重症胰腺炎的治疗有效率,减少并发症的发生,值得临床推广使用.     

突发训练伤现场救护有讲究

正目前,随着各单位实战化训练进入高潮,官兵突发训练伤不可避免。实施现场救护时,应当遵循先急后缓的原则。确保安全,先抢后救。使处于危险境地的战友尽快脱离险地,移至安全地带后再救治。摸准伤情,先重后轻。对呼吸异常、脉搏细弱或心跳停止、神志不清的伤病员,应立即采取急救措施,挽救生命;昏迷伤病员应注意维持呼吸道通畅;伤口处理一般应先止血,后     

3例髓质性海绵肾并发结石患者体外震波碎石治疗的护理

对3例髓质性海绵肾并发结石患者进行2次体外震波碎石(ESWL)治疗,术前留置输尿管导管(导管内注入液体石蜡),术后给予补液、排石、利尿及解痉治疗;对1例合并周期性低钾麻痹患者术前、术后及时补钾.结果治疗1～2周后摄尿路平片示:双肾、输尿管无残留结石.提示ESwL治疗髓质性海绵肾损伤小,安全有效;优质护理是获得良好效果的保障.     

机械通气辅助治疗急性心肌梗死合并重症左心衰竭的 效果观察

目的 探讨和分析机械通气辅助治疗心肌梗死合并重症左心衰竭的临床疗效.方法 在急性心肌梗死合并重症左心衰竭并接受治疗的患者中,选取70例作为研究对象.按照随机原则将其分为两组,其中35例在予以常规药物治疗的基础上结合机械通气治疗,为观察组;其余35例仅予以常规药物治疗,为对照组.在治疗结束后比较两组治疗有效率和治疗前后血压(SBP、DBP)、血氧饱和度(SaO2)、呼吸频率(RR)、心率(HR)的改善情况.结果 观察组患者治疗后血压(SBP、DBP)、血氧饱和度(SaO2)、呼吸频率(RR)、心率(HR)的改善情况显著优于对照组,差异具有统计学意义(P<0.05).结论 机械通气辅助治疗急性心肌梗死合并重症左心衰竭可取得显著疗效,改善患者的呼气困难状况.