输卵管造影所致血管栓塞抢救成功1例

输卵管造影是一种X线下对子宫、输卵管位置、大小、形态以及输卵管是否通畅进行直观观察的诊断方法；此外，造影剂对输卵管尚有扩张及润滑作用，可使不通畅的输卵管通畅，故输卵管造影可作为临床诊断和治疗的重要方法。我们在行输卵管造影时碘化油逆行进入血管形成油栓1例，经及时抢救病人转危为安。     

猫爪草多糖的理化性质及其对ANA-1细胞的免疫调节作用

目的探讨猫爪草多糖(PRT)对半贴壁巨噬细胞株ANA-1细胞免疫功能的影响。方法采用水提醇沉法提取和分离PRT;红外光谱法测定PRT分子结构;MTT法检测ANA-1细胞增殖能力;不同浓度的猫爪草多糖作用于脂多糖(LPS)诱导的ANA-1细胞,采用Griess法检测一氧化氮(NO)生成量;一氧化氮合酶(i NOS)测定试剂盒检测活性;RT-PCR法检测i NOS、白介素-6(IL-6)和肿瘤坏死因子α(TNF-α)m RNA水平的表达。结果得到灰白色粉末状猫爪草多糖,多糖含量98.17%;红外光谱显示PRT的糖苷键为α-构型,具有吡喃型糖环;PRT增强ANA-1细胞增殖能力,上调IL-6和TNF-αm RNA的表达,且浓度为200μg/m L时作用最强;PRT呈浓度依赖性抑制LPS诱导的ANA-1细胞分泌NO和i NOS活性,当多糖浓度为100μg/m L时,能够完全抑制i NOS m RNA的表达。结论 PRT能够增强ANA-1细胞增殖能力,上调IL-6和TNF-αm RNA水平的表达,抑制LPS诱导的ANA-1细胞分泌NO和i NOS的表达。     

疣状胃炎与幽门螺旋杆菌感染的关系及其临床治疗的探讨

目的探讨幽门螺旋杆菌（HP）及其产毒株（Tox＋HP）在疣状胃炎发生中的作用,了解胃黏膜肠上皮化生及上皮内瘤变在疣状胃炎（VG）时的发生情况,观察药物（质子泵抑制剂,三联疗法抗HP）及内镜下微波疗法对于疣状胃炎的确切疗效。方法通过胃镜检查将疣状胃炎分为成熟型（MVG）和非成熟型（UMVG）两类,每例均进行病理观察,HP感染分为HP感染阴性和阳性,根据以上情况将患者分为8组,分别予以药物治疗及微波治疗,比较各组HP及Tox＋HP感染、胃黏膜肠上皮化生及上皮内瘤变及不同疗法的效果情况。结果疣状胃炎时HP感染率高于对照组,以Tox＋HP感染为主;胃黏膜肠上皮化生及上皮内瘤变在成熟型胃炎组明显高于对照组,UMVG组稍高于对照组,但是无显著性差异;对于UMVG,单纯药物治疗1月治愈好转率达88％～94％,微波疗法治愈好转率近100％;对于MVG,单纯药物治疗1月治愈好转率达28％～35％,微波疗法治愈好转率近100％。结论HP尤其Tox＋HP的感染在疣状胃炎的发生中起了重要的作用,在疣状胃炎尤其MVG时胃黏膜肠上皮化生及上皮内瘤变发生率高于对照组,说明疣状胃炎有癌变倾向;单纯药物（质子泵抑制剂,三联疗法抗HP）疗法对于UMVG有效,对于MVG疗效差;内镜下微波疗法适用于MVG及药物不理想的UMVG患者。     

巧妙预防宝宝“猫抓热”

小华“感冒”了 开心的日子并不长,那天,妈妈发现小华有点儿打蔫儿,虽然小猫咪“喵喵”地叫着,他却懒得理睬。妈妈用手一摸,孩子发烧了。妈妈懂点儿育儿常识。所以也没慌张,给孩子喂了退烧药,多喝了点水,就哄着孩子睡觉了。可是第二天起来,孩子仍然在发烧。还有一点儿咳嗽,这回妈妈决定带孩子到医院去打针。可是打了几天抗生素,发烧总是反反复复,这回妈妈着急了,决定到医院详细检查。     

细菌细胞壁人髓系细胞触发受体-1天然配体的研究

Objective To look for the natural ligand(s) of human triggering receptor expressed on myeloid cell-1 (TREM-1), in order to provide the theoretical basis for elucidation of the pathogenesis of sepsis.Methods Neutrophils and monocytes isolated from human peripheral blood were treated with heat-inactivated Staphylococcus aureus , Pseudomonas aeruginosa , Mycobacterium tuberculosis, Staphylococcus aureus L-form or Pseudomonas aeruginosa L-form respectively for 24 hours.The cell wall was extracted from Staphylococcus aureus, Pseudomonas aeruginosa and Mycobacterium tuberculosis by ultrasound.Neutrophils and monocytes were isolated and treated with the cell wall respectively for 24 hours.Neutrophils and monocytes were isolated and treated with three main components from bacterial cell wall (polysaccharides,lipids and proteins) respectively for 24 hours.The level of TREM-1 mRNA was measured with fluorescent quantitative polymerase chain reaction (PCR), and the concentrations of tumor necrosis factor-α (TNF-α)and interleukin-1β (IL-1β) were measured with enzyme-linked immunosorbent assay (ELISA).Results The TREM-1 mRNA level and the concentrations of TNF-α and IL-1β in cell supernatant of neutrophils and monocytes were upgraded when treated with cell, cell wall and cell wall polysaccharides of Staphylococcus aureus and Pseudomonas aeruginosa.Compared with the blank control group, the TREM-1 mRNA level of neutrophils and monocytes was upgraded to (3.86± 0.20)-fold and (5.15±0.56)-fold respectively when treated with cell wall polysaccharides of Staphylococcus aureus (both P＜0.05);the TREM-1 mRNA level of neutrophils and monocytes was upgraded to (4.03±0.15)-fold and (7.22±0.73)-fold respectively when treated with cell wall polysaccharides of Pseudomonas aeruginosa (both P＜0.05).The effect could be attenuated by the addition of LP17 which could bind TREM-1 ligand.This attenuating effect was not found when the cells were treated with cell, cell wall or cell wall polysaccharides of Mycobacterium tuberculosis.Conclusion The study provides the evidence that TREM-1 natural ligand (s) is present on cell wall of bacteria including Staphylococcus aureus and Pseudomonas aeruginosa, and it might be polysaccharides.     

循环增强荧光分析仪及配套降钙素原定量检测试剂盒的分析性能评价

目的:对一款新近研发的循环增强荧光分析仪及配套降钙素原(PCT)试剂盒进行系统性的性能评价,并对新版美国临床实验室标准协会(CLSI)文件的适用性进行验证.方法:依据目前最新版CLSI文件(EP5-A3、C28-A3和EP9-A3),对国产的循环增强荧光分析仪及配套PCT定量检测试剂盒与进口Roche公司COBAS e...     

低能量损伤继发髋部骨折老年患者再发对侧骨折危险因素及风险预测效能研究

目的 探讨低能量损伤继发髋部骨折老年患者再发对侧骨折危险因素并分析风险预测效能,旨在为后续再发对侧骨折预防及个性化干预方案制定提供更多参考。方法 回顾性纳入2012年1月—2019年6月于鹤壁市人民医院就诊并行手术治疗低能量损伤继发髋部骨折老年患者共942例,根据术后36个月内是否再发对侧骨折分为再发组(72例)和未再发组(870例),采用单因素和多因素分析评估老年患者再发对侧骨折独立危险因素;描绘ROC曲线评价上述危险因素用于再发对侧骨折预测临床效能。结果 低能量损伤继发髋部骨折老年患者共942例术后36个月内再发对侧骨折72例,再发率为7.64%;单因素分析结果显示,年龄、性别、合并慢性疾病(心律失常/心功能不全/脑血管病比例/外周血管病/慢性阻塞性肺疾病/消化性溃疡/肾脏疾病)发生情况、Charlson合并症指数、肺部感染发生率、泌尿系感染发生率、下肢深静脉血栓发生率、入院至结束手术时间、基线血清K⁺、Na⁺、Cl^-、D-二聚体水平及凝血酶原时间均与低能量损伤继发髋部骨折老年患者再发对侧骨折有关(P<0.05...