中西医结合治疗类风湿性关节炎75例

<正> 治疗方药 黄芪30g,当归、仙灵脾、羌活、独活、防风、川芎各10g,川断、牛膝、威灵仙、秦艽、丹参各15g,制乳香、制没药、甘草各6g,细辛3g。水煎服,每周3～5剂,连服6～8周。寒重加桂枝;热重加连翘、知母、熟地、鸡血藤。制首乌。西药芬必得2粒,2/d次或炎痛喜康片20mg,1/d次。也可用甲氨喋呤片剂2.5mg,隔日口服1片,或针剂5mg肌注,每周2次。4周后若无不良反应,剂量可增加到每周15～20mg,疗程16周。用药期间注意查血象和肝肾功能。多种维生素和抗贫血药亦可辅佐应用。     

塞来昔布对人鼻咽癌CNE-2细胞生长抑制及放射增敏研究

Objective To investigate the growth inhibition and radiosensitization of Celecoxib in hu-man nasopharyngeal carcinoma cell line CNE-2. Methods CNE-2 growth inhibition by Celecoxib was eval-uated by MTT method. Apoptosis-related changes in morphology were observed by transmission electron mi-croscopy (TEM). Cell cycle distribution and apoptosis rate were measured by flowcytometry (FCM). The ex-pression of COX-2 protein was observed by SP method after the treatment of Celecoxib. Cells were randomly planted into four groups: irradiation control(Ci), drug group(Cd), irradiation group(R), and Celecoxib plus irradiation group(D+R). Single irradiation of 2,4,6,8,and 10 Gy were administered for colonogenic assay. Cell cycle distribution and apoptosis rate were analyzed at 6 Gy irradiation. Results The growth of CNE-2 cell was inhibited by celecoxib in a dose-and time-dependent manner, the IC50 was 80 μmol/L After the treatment, cell ratio of GO and G, phases was increased (47.03±2.76 vs 56.17±1.95, t=4.68, P= 0.010), whereas the ratio of S and G2/M phases was decreased (33.07±1.86 vs 24.87±1.76, t=5.54, P = 0.010; 19.30±0.53: 17.73±0.83, t=2.75, P=0.050), and the apoptosis rate was increased (1.57±0.47:10.47±0.31, t = 27.39, P = 0.000) in a dose-dependent manner. Apoptosis with nuclear chromatin condensation, fragmentation and cell shrinkage was found by TEM. SP method showed that Celeib decreased COX-2 expression (17.48±0.34 vs 12.82±0.51,t=13.20,P =0.00). The sensitivity ratio(D0) was 1.15. FCM showed that the percentage of cells in G2/M phase was significanty more in R and D+R groups than in Ci and Cd groups (68.00±1.65,54.27±5.74,17.60±0.80,14.86±1.23, t=47.70,P=0.000; t=11.63, P=0.000), and also significantly different between R group and D + R group (t=3.99, P= 0.020). The apoptosis rate was higher in R and D + R groups than Ci and Cd groups(4.83±0.97,9.50± 1.35,1.33±0.86 and 2.28±0.42,t=4.67,P=0.010;t=8.81, P=0.000), D + R group than R group(t =4.85,P=0.010). Conclusions Celecoxib can markedly inhibit the growth and induce apoptosis in CNE-2 cells,which may depend on COX-2 pathway. Celeeoxib potently enhances the radiosensitivity of CNE-2 cells,which may due to the repair inhibit of radiation-induced DNA damage, inhibit of cell proliferation,and enhancement of cell apoptosis after irradiation.     

78例危重新生儿血糖变化临床分析

危重新生儿容易发生糖代谢紊乱,而血糖异常对新生儿神经系统则会造成损伤,现将本院新生儿病房收治的78 例危重新生儿血糖变化分析报告如下.     

急性肾功能衰竭应用因液透析疗法的护理

本文总结了我院自１９９年开展血液透析疗法以来，为急性肾功能不全的抢救与治疗提供了更有疗效的方法，使治愈率明显提高，死亡率明显下降。     

塞来昔布对人鼻咽癌CNE-2细胞生长抑制及放射增敏研究

Objective To investigate the growth inhibition and radiosensitization of Celecoxib in hu-man nasopharyngeal carcinoma cell line CNE-2. Methods CNE-2 growth inhibition by Celecoxib was eval-uated by MTT method. Apoptosis-related changes in morphology were observed by transmission electron mi-croscopy (TEM). Cell cycle distribution and apoptosis rate were measured by flowcytometry (FCM). The ex-pression of COX-2 protein was observed by SP method after the treatment of Celecoxib. Cells were randomly planted into four groups: irradiation control(Ci), drug group(Cd), irradiation group(R), and Celecoxib plus irradiation group(D+R). Single irradiation of 2,4,6,8,and 10 Gy were administered for colonogenic assay. Cell cycle distribution and apoptosis rate were analyzed at 6 Gy irradiation. Results The growth of CNE-2 cell was inhibited by celecoxib in a dose-and time-dependent manner, the IC50 was 80 μmol/L After the treatment, cell ratio of GO and G, phases was increased (47.03±2.76 vs 56.17±1.95, t=4.68, P= 0.010), whereas the ratio of S and G2/M phases was decreased (33.07±1.86 vs 24.87±1.76, t=5.54, P = 0.010; 19.30±0.53: 17.73±0.83, t=2.75, P=0.050), and the apoptosis rate was increased (1.57±0.47:10.47±0.31, t = 27.39, P = 0.000) in a dose-dependent manner. Apoptosis with nuclear chromatin condensation, fragmentation and cell shrinkage was found by TEM. SP method showed that Celeib decreased COX-2 expression (17.48±0.34 vs 12.82±0.51,t=13.20,P =0.00). The sensitivity ratio(D0) was 1.15. FCM showed that the percentage of cells in G2/M phase was significanty more in R and D+R groups than in Ci and Cd groups (68.00±1.65,54.27±5.74,17.60±0.80,14.86±1.23, t=47.70,P=0.000; t=11.63, P=0.000), and also significantly different between R group and D + R group (t=3.99, P= 0.020). The apoptosis rate was higher in R and D + R groups than Ci and Cd groups(4.83±0.97,9.50± 1.35,1.33±0.86 and 2.28±0.42,t=4.67,P=0.010;t=8.81, P=0.000), D + R group than R group(t =4.85,P=0.010). Conclusions Celecoxib can markedly inhibit the growth and induce apoptosis in CNE-2 cells,which may depend on COX-2 pathway. Celeeoxib potently enhances the radiosensitivity of CNE-2 cells,which may due to the repair inhibit of radiation-induced DNA damage, inhibit of cell proliferation,and enhancement of cell apoptosis after irradiation.     

绿色通道手术病人治疗与护理体会

绿色通道手术病人为急危重病人必须无条件地以最快的速度救治,救治中,涉及到医护人员、医疗机构、政府和社会。在新的历史时期,应将现行的医院紧急事件指挥系统、院前急救绿色通道建设和建立医院灾难医学救援体系结合起来,使严重多发伤病人在伤后1h内得到有效救治者的死亡率大大低于1h以后救治者。现将2002年12月～2004年2月收治的严重创伤绿色通道手术病人的救治和护理报告如下。     

中国慢性咳嗽研究进展与展望

中国于20世纪初开始了慢性咳嗽的发病机制、病因诊断与治疗方面的系统研究,取得了一系列成果;结合研究成果制订了中国《咳嗽的诊断与治疗指南》,并参与国际咳嗽指南的制订。通过指南的推广普及,国内慢性咳嗽的诊治水平有了显著的提高。展望未来,我们要加强慢性咳嗽的流行病学、慢性咳嗽的中枢调控、难治性慢性咳嗽的治疗等方面的研究,进一...     

妇产科手术中泌尿系统损伤的临床分析

目的分析了发生在妇产科传统经腹手术中的泌尿系统损伤20例,以防治手术中的泌尿系统损伤。方法总结2000年1月至2010年1月间手术中16例泌尿系损伤发生的原因、部位、诊断、处理及预后。结果 10年间在妇科手术中发生泌尿系统损伤的患者20例,占同期术者1.1%,其中12例为输尿管损伤,8例为膀胱损伤,损伤多发生在盆腔粘连、子宫内膜异位症、肿瘤浸润、输尿管受盆腔肿瘤推移变位导致解剖关系不清时。18例均于术中诊断、术中手术修补。结论术中应正确操作,如发生损伤,应明确诊断、及时处理。     

无菌物品临床流通环节质量控制分析

目的 了解医院无菌物品流通周转环节的污染情况,探讨预防控制措施.方法 按<医院消毒卫生标准>和<消毒技术规范>的要求对各周转环节及空气进行微生物监测.结果 供应室无菌柜内空气消毒质量优先临床各科室;无菌物品兑换容器及下送车的(存放柜)污染较为严重,护士的手是主要带菌者,菌落数>100cfu/cm2的标本有14份(14.6%);革兰氏阴性菌(78.1%)是主要污染菌.以大肠埃希茵(32.3%)为主,金黄色葡萄球菌是革兰氏阳性茵的主要致病菌(14.6%).结论 加强周转环节管理、规范洗手、严格遵守无菌操作规程和屏障护理,确保无菌物品质量是保障医疗、护理质量的关键.