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51.
改进血清铜蓝蛋白凝胶电泳氧化酶活性盐酸对苯二胺显示法 总被引:1,自引:1,他引:0
介绍一种改进的检测血清铜蓝蛋白(CP)的方法:不连续淀粉-琼脂糖混合凝胶电泳分离CP,利用其氧化酶活性使盐酸对苯二胺氧化,形成的紫兰色产物沉积于凝胶板上CP相应位置,可直接观察CP量的变化。通过对一例Wilson氏家族的CP的测定,对这种方法在检测血清CP中的意义和临床应用价值进行了讨论。 相似文献
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使用铜蓝蛋白ELISA法测定了矽肺大鼠及汉防己甲素(汉甲)、克矽平、磷酸羟基喹哌(抗矽Ⅰ号)及柠檬酸铝(柠铝)治疗的矽肺大鼠血清铜蓝蛋白(Cp)含量,并与微量法测定结果进行了比较。两种方法测定结果十分相近。ELISA法对血清存放时间要求较宽,Cp的微量测定法简便准确,但要求血清存放时间短。 相似文献
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胃肠肿瘤标记物图像定量形态学研究进展徐少勇(郧阳医学院附属医院内科十堰442000)胃肠肿瘤标记物检测是当前肿瘤研究的热点,通过对标记物形态变化的研究可使肿瘤诊断更为特异,目前国内外对胃肠肿瘤标记物的研究趋于进行定量和数值化分析,早期的研究主要是采用... 相似文献
56.
Binding and inhibition of myeloperoxidase (MPO): a major function of ceruloplasmin? 总被引:5,自引:0,他引:5 下载免费PDF全文
M SEGELMARK B PERSSON T HELLMARK J WIESLANDER 《Clinical and experimental immunology》1997,108(1):167-174
Interactions between plasma proteins and MPO were studied. The protein fraction of normal plasma and serum was shown to exhibit an inhibitory effect on the peroxidase activity of MPO. Most of the inhibitory effect could be retained on an MPO-coupled affinity chromatography column. In particular, a protein with apparent mol. wt of 130 kD showed affinity for MPO. The protein was identified as ceruloplasmin by N-terminal amino acid sequencing and immunochemistry. During separation procedures the peroxidase inhibitory effect was limited to ceruloplasmin-containing fractions of plasma. Purified ceruloplasmin inhibited the peroxidase activity of MPO in a concentration-dependent manner, and exhibited selective binding to MPO-coated microtitre plates. This binding could be inhibited by MPO dissolved in buffer. Correspondingly the binding of MPO to ceruloplasmin-coated plates could be blocked by ceruloplasmin in solution, showing a physical interaction to occur between the two proteins under physiological conditions. We also found affinity to exist between MPO and C3 (and its C3d-containing fragments). However, C3 and C3 fragments did not inhibit the peroxidase reaction in vitro. We propose that ceruloplasmin takes part in the clearance and inactivation of MPO, in vivo.We also speculate that impaired inactivation of MPO may have a pathophysiological role in inflammatory diseases characterized by autoantibodies to MPO, such as rapidly progressive glomerulonephritis with P-ANCA (perinuclear anti-neutrophil cytoplasmic antibodies). 相似文献
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对河南省某化工厂职业接触TNT作业工人现场劳动卫生学调查和健康监护结果表明,车间空气中TNT浓度绝大多数超过MAC(1mg/m3),作业工人皮肤污染严重。TNT接触组与对照组血清GPT活性升高和HBsAg阳性检出率两组间无显著性差异,表明这两组人群肝功能属于正常范围。同时发现,TNT接触组和HBsAg阳性组血清铜蓝蛋白和谷胱甘肽过氧化物酶活性均极显著低于对照组(P<0.02,P<0.001);HBsAg阳性组这两项指标极显著低于TNT接触组(P<0.05,P<0.02)。表明TNT和HBV诱发的肝损伤机理可能部分归因与活性氧生成有关。 相似文献
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Serum ceruloplasmin (CP) was determined by electroimmunodiffusion method in 89 normal persons and 92 leukemic patients. It was found that the serum CP of acute and chronic leukemia was very significantly higher than normal (P<0.01). There were no signifcant difference in elevation of serum CP between various types of leukemia. During remission oJ acute or chronic leukemia, the serum CP decreased significantly (P<0.05), but still higher than normal (P<0.01). In the case of long-term remission, serum CP decreased to normal level. During relapse of acute leukemia or acute blast crisis of chronic leukemia, serum CP raised again significantly (P<0.05). Infection enabled the leukemic patient to further increase serum CP (P<0.01). In acute leukemia, serum CP level correlated positively with the percentage of blast cells in bone marrow (P<0.05). 相似文献
59.
目的 评价苯那普利对 2型糖尿病患者尿铜蓝蛋白 (Cp)及尿白蛋白 (Alb)的影响。 方法 84例 2型糖尿病患者均分为对照组、控制血压组和苯那普利组。记录及测定受试者的年龄、性别、糖尿病病程、血压、腰围、臀围、腰臀比、身高、体重、体重指数、血糖、糖化血红蛋白、胰岛素、尿素氮、肌酐、尿酸、血脂、血白蛋白 ,并留取晨尿测定铜蓝蛋白、白蛋白及肌酐 (Cr) ,随访 6个月后复查上述指标 ,比较尿Alb/Cr及尿Cp/Cr的变化。 结果 对照组无明显变化 ;控制血压组及苯那普利组尿Alb/Cr和尿Cp/Cr水平均下降 ,苯那普利组尿Cp/Cr下降较控制血压组更明显 (P <0 .0 5 )。结论 苯那普利可降低尿Cp/Cr及尿Alb/Cr。 相似文献
60.
B A Bassiouni A A Rafei 《European journal of obstetrics, gynecology, and reproductive biology》1979,9(2):81-88
Ceruloplasmin, the blue copper-protein of the blood plasma, is believed by some workers to be involved in the metabolic management of 5-hydroxytryptamine (serotonin) during pregnancy. 5-Hydroxytryptamine is abortifacient in experimental animals. By some authors, a role has been suggested for it in human abortion. The plasma concentrations of 5-hydroxytryptamine, copper and ceruloplasmin were measured in non-pregnant women, in normal early pregnancy and in cases of spontaneous abortion. Compared with normal early pregnancy, cases of inevitable abortion show lower values for both plasma copper and ceruloplasmin and higher values for plasma 5-hydroxytryptamine. The possible implications of these findings are discussed in view of the alterations in ceruloplasmin values in pregnancy and in the light of what is known of the pharmacology and metabolism of 5-hydroxytryptamine. It it believed that these alterations in plasma copper, ceruloplasmin and 5-hydroxytryptamine in cases of inevitable abortion are the effect, rather than the cause, of abortion. 相似文献