心理应激的实验方法

本文就心理应激研究中所采用的实验方法作一综述。     

抗中性粒细胞胞质抗体相关性血管炎的研究现状

抗中性粒细胞胞质抗体(ANCA)是存在于系统性血管炎患者中的一类自身抗体,其抗原是多形核粒细胞和单核细胞的胞质成分.除此之外,微型多动脉炎、局限性肾动脉炎等的发病与ANCA亦密切相关.临床上将上述疾病称为ANCA相关系统性血管炎(AASV).AASV肾脏损害的表现主要包括少尿、血尿和蛋白尿,伴肾小球滤过率下降.目前诊断主要依赖肾脏病理学检查,其特征性病理表现为寡免疫复合物沉积的局灶节段坏死性肾小球肾炎,形成以巨噬细胞和上皮细胞为主要细胞成分的新月体.AASV预后不良的因素包括老年、肾功能严重受损.肾脏病理为判断预后提供更多的依据,肾间质纤维化提示预后不良.     

作家王蒙善养生

当记者走进王蒙的家,见到靠在沙发上聚精会神观看动画节目的王蒙时,似乎很难产生“肃然起敬”的感觉,倒是咂摸出几许淡淡的亲切。 王蒙的夫人崔瑞芳女土沏上茶后,嗔怪道:“都60多岁的人了,还喜欢瞅动画片。”可见身为中国严肃文学代表作家的王蒙,同时也是一名普     

作家在家有暇无暇——访著名作家萧乾先生

萧乾老先生的家在北京西城一幢普通的居民楼里。他的门上贴有两张醒目的纸条:“本人现在健康情况不宜聊大天”,“年迈多病,仍赶任务,题词作序,一概谢绝。”当记者叩响他老人家的房门时,出来的,仍是那尊笑咪咪的弥勒佛模样的长者。一见面我就被他那张慈祥微笑的面孔所吸引:他双眼弯弯,嘴角上翘,头顶几根银丝,闪闪发亮,大大的圆脸盘,充满和善之意,可爱又可敬。 萧老今年已88岁高龄,尽管他去年住了一次     

人工补片在恶性肿瘤人工关节置换修补中的临床应用

目的：对恶性肿瘤假体置换后的患者应用人工补片修复关节囊，探讨其方法和临床应用的价值。方法：回顾总结2003年8月～2005年4月肩、髋和膝关节周围恶性肿瘤病例10例，男7例，女3例；年龄43-65岁，平均52岁。病程3个月～2年，平均1年2个月。肿瘤生长在肱骨上段4例，股骨上段5例，胫骨上段1例。术前、术后10例患者患病关节功能按M1卵关节功能评价系统评定。手术方法：所有患者均实施肿瘤广泛切除加入工关节假体置换术后用人工补片修复关节囊．并进行随访观察。对患者术前和术后的临床结果进行分析评价。结果：全部患者平均随访7个月．10例患者术后伤口引流管平均于术后5天拔除，伤口均Ⅰ期愈合，无术后感染并发症，未发生假体脱位。术前关节功能按MTST关节功能评价系统测定6例为良、4例为可；而术后8为良、2例为可。所有患者最后随访时对治疗结果满意。结论：应用人工补片修复肿瘤关节假体置换后的关节囊，对于其稳定性和动力重建起到了重要的作用．临床效果满意。     

氯沙坦对肾移植术后受者血红蛋白的影响及其使用的安全性

Objective To evaluate the influence of losartan on levels of hemoglobin (Hb) in patients after renal transplantation. The safety of losartan in these patients was also evaluated. Methods Sixty-six hypertensive patients after renal transplantation with stable allograft function and serum creatinine levels below 176. 8 μmol/L (2 mg/dl) were divided into treatment group (n = 34) treated with losartan at a dose of 50 mg/d, and control group (n = 32) not treated with losartan. Each participant was followed up for 6 months, and differences in Hb, whole blood CsA trough concentration, blood pressure (BP), serum creatinine (Cr) and GFR were compared at baseline, and at the month 1, 2, 3 and 6. Results Serum Cr in treatment group showed a slight, hut significant increase at the month 1 (P<0.05 vs baseline), then gradually returned to the baseline until the month 6. Relatively, the estimated glomerular filtration rate (GFR) in treatment group was decreased slightly and temporarily. The Fib level in treatment group kept on dropping in the first two months (P <0.01 vs baseline), then remaining stable since the month 3. An uptrend of Hb was seen in the control group hut there was no statistically significant difference. For patients with Hb≥160 g/L, a progressive decrease in Hb was observed after losartan treatment (P<0.01 vs baseline), while a slight, non-significant decrease in the controls. For patients with Hb <160 μmol/L, Hb in treatment group was decreased in the first two months (P<0.01 vs baseline at the month 2), then gradually increased to baseline from the month 3 to 6. A progressive increase in Hb was seen in control group (P < 0.05 vs baseline). Conclusion Losartan can effectively and safely relieve and prevent posttransplantation erythrocytosis following renal transplantation.     

氯沙坦对肾移植术后受者血红蛋白的影响及其使用的安全性

Objective To evaluate the influence of losartan on levels of hemoglobin (Hb) in patients after renal transplantation. The safety of losartan in these patients was also evaluated. Methods Sixty-six hypertensive patients after renal transplantation with stable allograft function and serum creatinine levels below 176. 8 μmol/L (2 mg/dl) were divided into treatment group (n = 34) treated with losartan at a dose of 50 mg/d, and control group (n = 32) not treated with losartan. Each participant was followed up for 6 months, and differences in Hb, whole blood CsA trough concentration, blood pressure (BP), serum creatinine (Cr) and GFR were compared at baseline, and at the month 1, 2, 3 and 6. Results Serum Cr in treatment group showed a slight, hut significant increase at the month 1 (P<0.05 vs baseline), then gradually returned to the baseline until the month 6. Relatively, the estimated glomerular filtration rate (GFR) in treatment group was decreased slightly and temporarily. The Fib level in treatment group kept on dropping in the first two months (P <0.01 vs baseline), then remaining stable since the month 3. An uptrend of Hb was seen in the control group hut there was no statistically significant difference. For patients with Hb≥160 g/L, a progressive decrease in Hb was observed after losartan treatment (P<0.01 vs baseline), while a slight, non-significant decrease in the controls. For patients with Hb <160 μmol/L, Hb in treatment group was decreased in the first two months (P<0.01 vs baseline at the month 2), then gradually increased to baseline from the month 3 to 6. A progressive increase in Hb was seen in control group (P < 0.05 vs baseline). Conclusion Losartan can effectively and safely relieve and prevent posttransplantation erythrocytosis following renal transplantation.     

青霉素双黄连抗金黄色葡萄球菌感染实验比较

青霉素双黄连抗金黄色葡萄球菌感染实验比较林海清程明宋钦兰山东中医药大学附院（２５００１１）关键词：青霉素／双黄连／抗菌实验１药品配制１．１儿药品：青霉素山东鲁抗制药厂批号９５０７０９双黄连中国哈尔滨中药二厂批号９５０５１８金黄色葡萄球菌山东医科大学生...     

miR⁃588在乳腺癌中的表达及对乳腺癌细胞增殖和侵袭的作用

目的：探讨miR?588在乳腺癌中的表达及对乳腺癌细胞增殖、迁移和侵袭能力的影响。方法：用化学合成的miR?588成熟模拟物（miR?588 mimic）过表达miR?588,并验证miR?588过表达率;采用MTT、克隆形成实验检测miR?588对乳腺癌细胞MCF7和MDA?MB?231增殖的影响;采用划痕愈合实验和Transwell侵袭实验检测miR?588对MDA?MB?231细胞迁移和侵袭的影响。结果：miR?588在乳腺癌组织及细胞中的相对表达均低于癌旁正常组织及乳腺正常上皮细胞;过表达miR?588能明显抑制MCF7和MDA?MB?231细胞的增殖;上调miR?588可显著抑制MDA?MB?231细胞的迁移和侵袭能力。结论：miR?588在乳腺癌中低表达,过表达miR?588可抑制乳腺癌细胞的增殖和侵袭。