获得性免疫缺陷综合征患者胃黏膜组织人类免疫缺陷病毒感染分析

Objective To investigate the distribution and amount of human immunodeficiency virus (HIV) infection in gastric mucosa from untreated acquired immune deficiency syndrome (AIDS) patients and highly active antiretroviral therapy (HAART) treated patients.Methods Thirty-five AIDS patients (14 untreated patients and 21 patients receiving HAART) and 10 HIV-1 seronegative patients with gastrointestinal symptoms were enrolled and examined by upper endoscopy.The labeled HIV-1 double-stranded cDNA probe was a PCR product corresponding to the LTR and gag gene of the HIV-1 genome.HIV in gastric mucosal tissues from AIDS patients was detected using in situ hybridization (ISH) and compared with that in peripheral blood mononuclear cells (PBMC).Results ① No obvious character was found in gastrointestinal symptoms,endoscopy examination and pathology results of AIDS patients.② The expression of HIV gene was mainly detected in the gastric mucosal mononuclear cell (MMC).Other cells were also observed with HIV expression including mucosal epithelial cells,gland epithelial cells and interstitial cells.③There was no difference in HIV expression between sinus ventriculi and gastric body.④ HIV gene expression from AIDS patients was (1.97±3.25)% in gastric mucosa,no difference in HIV gene expression between two groups (P＞0.05).⑤ HIV gene expression in PBMC smear from AIDS patients was (12.38 ± 9.17)%.HIV expreesion in PBMC from patients who had received HAART for 1-4 years were markedly lower than that from patients who had not received HAART (P＜0.05).Conclusions The gastric mueosa is one of HIV infected sites.The potential effect of HAART on the decrease of HIV infected cells in gastric mucosa was unsatisfactory.     

加强手术室安全管理防范差错事故

随着社会的进步，人们生活文化水平的提高，患者的法律观念。经济意识和自我保护观念也不断增强。手术室是医院对患者实施手术治疗并担负抢救工作的重要场所，与患者的生命息息相关，任何疏忽大意都可能酿成严重的后果而带来终生的遗憾。结合实际情况，现将手术室护理工作中的安全隐患及防护对策总结如下：     

ABO血型正反定型不符情况分析

目的：分析血型鉴定中ABO正反定型不符的原因。方法用患者血样做ABO正反定型鉴定,正反定型结果不符合时采取相应对策正确鉴定ABO血型。结果20例ABO血型正反定型不符的患者标本经重新鉴定后,冷凝集为4例, ABO亚型8例, AB抗原性减弱2例,存在不规则抗体6例。结论造成ABO血型正反定型不符的主要原因是ABO亚型和不规则抗体,为此在输血前要正确鉴定患者ABO血型,确保输血安全。     

北京市社区卫生服务中心公用经费补偿研究

目前北京市实行收支两条线的社区卫生服务中心(站)公用经费的补偿存在较大问题,文章针对现行存在的问题,对社区公用经费的补偿问题进行研究,规范了公用经费的定义、范围,对公用经费的补偿形式及拨款程序提出了建议,并对公用经费的定额项目标准制定提出了方法。     

视网膜分支静脉阻塞并黄斑水肿综合疗法108例疗效分析

目的：观察分析综合治疗视网膜分支静脉阻塞并黄斑水肿的疗效.方法：经眼底荧光造影确诊为视网膜分支静脉阻塞并黄斑水肿的患者108例（108眼）,使用综合疗法治疗.综合疗法即中药复方血栓通胶囊口服（1.5g,tid）及曲安奈德（40mg）球后注射治疗联合激光治疗.随访6个月,对比分析治疗前及治疗后视力,眼底荧光造影检查,黄斑OCT（黄斑厚度分析）的情况.结果：与治疗前相比,治疗后1个月,3个月及6个月,视力提高者分别为52眼（48.15%）,78眼（72.22%）,99眼（91.67%）;治疗后眼底荧光造影示治愈的分别为79眼（73.15%）,94眼（87.04%）,98眼（90.74%）,黄斑中心凹厚度治疗前与治疗后不同时期配对t检验,p〈0.05,差异具有统计学意义. 结论：综合疗法对视网膜分支静脉阻塞并黄斑水肿,有显著疗效.