柱前衍生化HPLC法建立苦豆子多糖特征图谱的研究

[目的]建立苦豆子多糖特征图谱。[方法]采用1-苯基-3-甲基本5-吡唑酮(PMP)柱前衍生化高效液相色谱(HPLC)法对苦豆子多糖进行单糖组成分析。[结果]苦豆子多糖特征图谱由7个共有峰组成,为甘露糖、葡萄糖、鼠李糖、半乳糖、木糖、阿拉伯糖以及1个未鉴定出的单糖。[结论]柱前衍生化HPLC法建立多糖的特征图谱操作简便、准确、重复性良好,为苦豆子多糖的质量评价提供了依据。     

基质金属蛋白酶-9及其抑制剂在多发性硬化发病中的作用

目的探讨多发性硬化(multiple sclerosis,MS)患者血清基质金属蛋白酶-9(matrix metallopmteinases-9,MMP-9)、金属蛋白酶组织抑制剂-1(tissue inhibit or ofmetalloproteinases-1,TIMP-1)水平及MMP-9/TIMP-1比值变化及与临床的关系。方法应用酶联免疫吸附法(enzynis-finked immunoadsonlent assay,ELISA)测定32例MS患者及20名健康对照组(health control,HC)血清MMP-9、TIMP-1水平,计算MMP-9/TIMP-1比值,并分别进行统计学分析。结果MS患者血清MMP-9水平及MMP-9/TIMP-1值较健康对照组显著升高,而TIMP-1水平则显著降低,参异均有统计学意义(P0.01),MRI钆(galolinium,Gd)强化扫描阴性(Gd-)MS患者较钆强化扫描阳性(Gd+)MS患者血清MMP-9水平及MMP-9/TIMP-1值显著降低(P0.01),但仍显著高于健康对照组(P0.05),而TIMP-1水平则显著升高(P0.01),同时仍显著低于健康对照组(P0.05)。结论MMP-9、TIMP-1参与了MS的发病,MMP-9水平升高及TIMP-1水平降低与MS活动有关,MMP-9/TIMP-1比值可作为监测MS活动性的生物学指标。     

Neural circuits and temporal plasticity in hindlimb representation of rat primary somatosensory cortex: revisited by multi-electrode array on brain slices

Objective

The well-established planar multi-electrode array recording technique was used to investigate neural circuits and temporal plasticity in the hindlimb representation of the rat primary somatosensory cortex (S1 area).

Methods

Freshly dissociated acute brain slices of rats were subject to constant perfusion with oxygenated artificial cerebrospinal fluid (95% O₂ and 5% CO₂), and were mounted on a Med64 probe (64 electrodes, 8×8 array) for simultaneous multi-site electrophysiological recordings. Current sources and sinks across all the 64 electrodes were transformed into two-dimensional current source density images by bilinear interpolation at each point of the 64 electrodes.

Results

The local intracortical connection, which is involved in mediation of downward information flow across layers II–VI, was identified by electrical stimulation (ES) at layers II–III. The thalamocortical connection, which is mainly involved in mediation of upward information flow across layers II–IV, was also characterized by ES at layer IV. The thalamocortical afferent projections were likely to make more synaptic contacts with S1 neurons than the intracortical connections did. Moreover, the S1 area was shown to be more easily activated and more intensively innervated by the thalamocortical afferent projections than by the intracortical connections. Finally, bursting conditioning stimulus (CS) applied within layer IV of the S1 area could successfully induce long-term potentiation (LTP) in 5 of the 6 slices (83.3%), while the same CS application at layers II–III induced no LTP in any of the 6 tested slices.

Conclusion

The rat hindlimb representation of S1 area is likely to have at least 2 patterns of neural circuits on brain slices: one is the intracortical circuit (ICC) formed by interlaminar connections from layers II–III, and the other is the thalamocortical circuit (TCC) mediated by afferent connections from layer IV. Besides, ICC of the S1 area is spatially limited, with less plasticity, while TCC is spatially extensive and exhibits a better plasticity in response to somatosensory afferent stimulation. The present data provide a useful experimental model for further studying microcircuit properties in S1 cortex at the network level in vitro.     

S100蛋白在儿童髓母细胞瘤组织中的表达及与预后的关系

目的 探讨S100蛋白在儿童髓母细胞瘤组织中的表达,分析与肿瘤临床特点之间的关系和对儿童髓母细胞瘤预后的影响.方法 应用免疫组化SP法检测S100蛋白在44例儿童髓母细胞瘤中的表达,并结合长期随访资料进行生存曲线分析.结果 S100阳性表达率为68.18%.S100表达与儿童髓母细胞瘤患者性别、年龄、有无侵犯四脑室、有...     

子痫前期或子痫致可逆性后部脑病综合征的临床及影像学分析

目的 探讨子痫前期或子痫致可逆性后部脑病综合征( posterior reversible encephalopathy syndrome,PRES)的临床及影像学特点.方法 选择子痫前期或子痫致PRES患者21例,采用回顾性分析方法对临床症状及脑磁共振成像(magnetic resonance imaging,MRI)资料进行分析.结果 主要临床症状:21例患者中癫痫发作18例,头痛16例,意识状态改变15例,视觉障碍12例;PRES病灶分布:顶、枕叶20例,额叶14例,颞叶11例,基底节区11例,胼胝体压部4例,小脑半球3例,脑干1例;PRES病灶分布模式:经典型7例,全脑型7例,额上沟型6例,部分或不对称型1例.结论 子痫前期或子痫致PRES临床症状典型,除顶、枕叶外,额叶、颞叶、基底节区等部位累及常见,多见3种主要病灶分布模式,准确识别其影像学表现对正确诊断具有重要意义.     

基于Hypogen药效团模型发现潜在的PTP1B抑制剂

目的 采用计算机辅助药物设计的方法发现潜在的PTP1B抑制剂。方法 应用3D-QSAR药效团模型中的Hypogen模块构建药效团模型，成本分析、测试集预测和Fisher检验3种方法来验证该模型可用于预测化合物的生物活性的能力。运用该药效团模型对ZINC数据库进行虚拟筛选，得到Fit value值较高的先导化合物ZINC35671983。根据药效团的特征对ZINC35671983进行结构改造得到相应化合物。将化合物用ADMET进行成药预测。结果 ZINC35671983进行结构改造筛选获得92个化合物，筛出对接得分高于ZINC3567198的8个化合物。结论 发现8个潜在的PTP1B抑制剂，这有助于发现新的PTP1B先导化合物。     

Id2shRNA慢病毒载体的构建及其转染后对胶质瘤细胞增殖和凋亡的影响

目的 构建能高效敲减分化抑制因子2 (Id2) 基因的短发夹状小干扰RNA(shRNA)慢病毒载体,并观察敲减Id2对胶质瘤细胞株U251增殖和凋亡的影响.方法 设计并合成特异性针对Id2基因的shRNA序列,将其构建到慢病毒载体pGCSIL-GFP中.以含有Id2基因的质粒为模板,扩增Id2基因cDNA序列的特异性片...     

山东省农村地区食物摄入与自杀未遂的关系研究

目的：研究山东省农村人群中的自杀未遂与食物摄入的关系。方法：选取山东邹城、莒南、章丘、新泰和沂水5个县农村地区的自杀未遂者作为病例组，同期选择性别和年龄（±3岁）相匹配的同村或邻村居民作为对照组，每组132例。采用面对面、一对一的访谈方式和问卷收集研究对象的相关信息。采用多因素条件logistic回归模型分析食物摄入与自杀未遂的关系。结果：调查对象中位年龄40岁（31～46岁），两组女性均占63.6%。与对照组相比，自杀未遂者个人年收入更低或更多存在身体疾病和精神障碍等（P<0.05）。单因素分析结果显示，水果和茶的摄入与自杀未遂有关（P<0.05），而海鲜、红肉和豆制品与自杀未遂无关（P>0.05）。调整婚姻状况、职业、个人年收入、身体疾病、精神障碍和其他食物摄入等影响因素后，水果摄入≥3 d/周为自杀未遂的保护因素（OR=0.37,95%CI:0.15～0.92），但茶、海鲜、红肉和豆制品摄入与自杀未遂无关（P>0.05）。结论：水果摄入≥每周3 d是自杀未遂的保护因素，茶、海鲜、红肉和豆制品的摄入与自杀未遂无关。     

医疗机构肺结核可疑者转诊未到位的原因分析

通过因症就诊及时发现肺结核病患者,尤其是及时发现涂阳肺结核病患者,已是公认有效和可行的病例发现方式[1]。对综合医院诊断的肺结核或疑似肺结核病例采取转诊追踪措施,是确保75%结核病患者发现率的一项长期技术措施[2]。因此,