IgG Against Dengue Virus in Healthy Blood Donors,Zanzibar, Tanzania

We conducted a seroprevalence survey among 500 healthy adult donors at Zanzibar National Blood Transfusion Services. Dengue virus IgG seroprevalence was 50.6% and independently associated with age and urban residence. These data will aid in building a surveillance, preparedness, and response plan for dengue virus infections in the Zanzibar Archipelago.Key words: dengue, seroprevalence, Zanzibar, viruses, vector-borne infections     

835例孕妇24h动态心电图心律失常分析

目的研究在常规静态心电图检查中发现有房早、室早、一、二度房室传导阻滞,窦性心动过缓等心律失常的孕妇,做动态心电图检查是否有更进一步发现。方法记录和分析835例孕妇动态心电资料,分析24h心律和心率,进行资料分析。结果妊娠早期检出心律失常79阵次;中期检出心律失常84阵次;晚期130阵次。835例孕妇均为窦性心律,24h平均心率为87,心率范围为:56～163次/分,心律失常检出达100%;100%检出有窦性心动过速,18%检出房早,室早检出律7%,成对室早检出1%。结论随着孕周增加,孕妇心律失常检出律增加。     

392例围生儿死亡分析

目的通过对围生儿死亡原因进行分析,探讨相应对策,为制定科学有效的降低围生儿死亡率的措施提供依据。方法回顾性分析2001～2010年我院392例围生儿死亡病例的相关资料。结果我院围生儿死亡率为13.44‰。围生儿死亡主要原因依次为:脐带因素、胎儿畸形、早产、胎盘因素、羊水过少、重度子痫前期、妊娠合并梅毒。结论要进一步降低围生儿死亡率,必须以加强围生育期保健为重点,同时着力提高孕产妇保健意识和自我监护能力。     

Sperm quality improvement after date seed oil in Vitro supplementation in spontaneous and induced oxidative stress

In vitro supplementation with date seed oil （DSO） can protect spermatozoa against hydrogen peroxide （HiO2）- mediated damage and can improve sperm function, possibly owing to antioxidant properties. We tested the antioxidant effects of DSO on human sperm motility, sperm viability, reacted acrosome and lipid peroxidation assessed in vitro after H202-mediated oxidative damage in spermatozoa. Sixteen patients （mean age： 35 years; range： 25-45 years） referred to the Histology-Embryology Laboratory of the Medicine Faculty of Sfax for semen analysis after 12-24 months of sexual intercourse without conception were selected. After spermiogram, sperm selection by twointerface discontinuous Sill Select gradient was performed, and selected spermatozoa were used in four experimental assays： control; incubation with 100um H2O2; incubation with 0.1% DSO; and co-incubation with 0.1% DSO and 100 um H2O2. Motility and viability were determined using World Health Organization criteria. Acrosome reaction and lipid peroxidation were assessed by staining with fluorescein isothiocyanate-Pisum sativum and spectrophotometric measurement of malondialdehyde, respectively. Results showed that incubation with H2O2 alone led to a significant increase in lipid peroxidation （57.83%, P 〈 0.05） associated with a significant decrease in sperm motility, sperm viability （after 30 min and 24 h） and percentage of reacted acrosome （P 〈 0.05）. Date seed oil im- proved sperm motility after 24 h of incubation （P 〈 0.05） and protected spermatozoa against the deleterious effects of H2O2 on motility, viability, acrosome reaction and lipid peroxidation. We conclude that supplementation with DSO may have a function in antioxidant protection against male infertility.     

医院环氧乙烷气体灭菌包保存期限的研究

目的 观察环氧乙烷气体灭菌及保存环境对无菌包保存期限的影响.方法 将960个无菌包.根据其大小、性质、轻重分别用不同规格的纸塑胶袋包装密封用环氧乙烷气体灭菌,出炉后立即送检128个包做细菌培养,其余832个包随机分为四组每组208个,分别存放于供应室无菌间物品存放架及病区一楼、二楼、四楼的专用无菌物品存放柜保存2年,于灭菌后第3、6、9、12、15、18、21、24个月每组每次随机抽取26个包送细菌培养;同时观察记录各组室内温度、相对湿度变化.结果 不同保存区域、不同温湿度(21.31～25.28℃;58.88%～69.83%)、不同时段送检的692个无菌包(因丢失、外包装损坏的140个视为无效包,不计算在内)均无细菌生长.结论 医院环氧乙烷气体灭菌包在不同保存区域、不同温湿度的条件下可无菌保存2年.     

Adhesive performance of precoated brackets after expiration

Objective:To evaluate adhesive performance in terms of debonding forces of precoated metal and ceramic brackets 4 years after expiration.Materials and Methods:Buccal and lingual surfaces of embedded extracted maxillary premolars were etched with 34% Tooth Conditioner Gel (Dentsply Caulk, Milford, Del), rinsed, and dried. Transbond MIP (3M Unitek, Monrovia, Calif) was applied prior to placing adhesive precoated brackets (APC II Victory stainless steel and APC Plus Clarity ceramic brackets, 3M Unitek). The preexpiration brackets had 29–35 months before, and the postexpiration brackets were 45–52 months past, their expiration dates. Sample size was 17–21 per group. Debonding forces were determined by subjecting the bonded brackets to a shear force in a universal testing machine. Debonding forces were compared using two-way ANOVA. Debonded surfaces were examined under a stereomicroscope to determine failure modes, which were compared using the chi-square test.Results:No statistically significant difference was found in debonding forces (P  =  .8581) or failure modes (P  =  .4538) between expired and unexpired brackets. Metal brackets required statistically significantly higher debonding forces than did ceramic brackets (P  =  .0001). For both expired and unexpired brackets, failure modes were mostly cohesive in the adhesive layer for ceramic brackets, and mixed between adhesive and cohesive failure in the adhesive layer for metal brackets.Conclusions:Adhesive precoated brackets did not have any reduction in enamel-adhesion properties up to 4 years after their expiration date. Extended shelf life testing for precoated dental brackets may be worth considering.     

“姜红大枣汤”在人工流产术中的应用效果观察

目的 探讨“姜红大枣汤”在人工流产术中的应用效果和意义。方法 随机选择50例术前口服温热“姜红大枣汤”治疗的人工流产病人为治疗组，44例采用常规人工流产术病人为常规治疗组，观察术前和术中血压、心率变化；术中、术后疼痛程度；人工流产综合征发生率；平均手术时间及阴道流血量。结果 治疗组中的血压、心率变化波动幅度小，疼痛减轻程度高，人流综合征的发生率低，与常规治疗组相比差异有显著性意义（P ＜ 0.05），两组平均手术时间和术中出血量比较差异无显著意义（P ＞ 0.05）。结论 口服“姜红大枣汤”预防和辅助治疗人流综合征效果显著，简便易行，无不良反应，病人易接受。     

胃肠激素检测在小于胎龄儿变化中临床意义

应用放射免疫法对68例小于胎龄儿(其中早产36例;足月32例)生后喂奶前及第7日空腹血中生长抑素(soma-tostatin,SS)、胃动素(motilin,MOT)、胃泌素(gastrin,Gas)浓度进行测定,并以30例足月新生儿作为对照组。结果小于胎龄儿组生后喂奶前及第7日空腹血浆胃动素、胃泌素均明显低于对照组,而生长抑素浓度明显高于对照组.并且与胎龄、开始喂养时间及当日进奶等因素相关。结论小于胎龄儿消化道机能适应胃肠道营养,在严密观察下应用合理的喂养方式早日开始胃肠道营养,将能促进小于胎龄儿胃肠道的发育和成熟。