植物花青素对肿瘤抑制作用的研究进展

植物花青素是一类可溶于水的类黄酮,主要存在于各种蔬菜水果中。常见的植物花青素主要分为天竺葵色素(Pelargonidin)、芍药色素(Peonidin)、矢车菊色素(Cyanidin)、锦葵色素(Malvidin)、矮牵牛色素(Petunidin)、飞燕草色素(Delphinidin)六种;植物花青素分子中含有共轭双键体系,并含有碱性或酸性基团,因而具有抗氧化、抗炎、调节免疫、抗衰老和抑癌等生物学作用,尤其以抑癌作用最为突出。因此,本文将对植物花青素的抑癌作用及可能的机制进行综述。     

Lactation mastitis: Promising alternative indicators for early diagnosis

Although lactation mastitis (LM) has been extensively researched, the incidence rate of LM remains a salient clinical problem. To reduce this incidence rate and achieve a better prognosis, early and specific quantitative indicators are particularly important. It has been found that milk electrolyte concentrations (chloride, potassium, and sodium) and electrical conductivity (EC) significantly change in the early stages of LM in an animal model. Several studies have evaluated EC for the detection of subclinical mastitis in cows. EC, chloride, and sodium content of milk were more accurate for predicting infection status than were other variables. In the early stages of LM, lactic sodium, chloride, and EC increase, but potassium decreases. However, these indicators have not been reported in the diagnosis of LM in humans. This review summarizes the pathogenesis and the mechanism of LM in terms of milk electrolyte concentration and EC, and aim to provide new ideas for the detection of sub-clinical mastitis in humans.     

Review of clinical characteristics,immune responses and regulatory mechanisms of hepatitis E-associated liver failure

Hepatitis E virus (HEV) is the most common cause of acute liver failure (LF) and one of the most common factors causing acute injury in acute-on-chronic LF (ACLF). When HEV-related LF occurs, a series of changes take place in both the intrahepatic environment and extrahepatic microenvironment. The changed types and distribution of immune cells (infiltrating macrophages and increased lymphocytes) in liver tissue, as well the increased proinflammatory cytokines and chemokines in the blood, indicate that the occurrence and progression of HEV-related LF are closely related to immune imbalance. The clinical features and immune reaction in the body during HEV-related acute LF (ALF) and ACLF are complicated. This review highlights recent progress in elucidating the clinical manifestations of HEV-associated ALF and ACLF and discusses the corresponding systemic immune changes and possible regulatory mechanisms.     

Botulinum toxin blocks mast cells and prevents rosacea like inflammation

Background

Rosacea is a chronic inflammatory skin condition whose etiology has been linked to mast cells and the antimicrobial peptide cathelicidin LL-37. Individuals with refractory disease have demonstrated clinical benefit with periodic injections of onabotulinum toxin, but the mechanism of action is unknown.

自然疗养因子的作用机制研究进展

自然疗养因子所涵盖的内容丰富,其生物学效应及形成复杂。现代疗养学在形成过程中相继提出自然疗养因子对机体作用的理论或假说。随着医学模式的转变和现代医学的发展,自然疗养因子作用机制研究从分子、细胞到组织器官,涉及机体物质代谢、神经系统、内分泌和免疫等功能的相互作用,形成整体的各个水平产生适应的作用机制;研究证实自然疗养因子的作用最根本的表现为改善机体内环境稳态和机体与外环境间的平衡,是靠机体的适应性反应机制实现的;其次与祖国医学“天人合一”整体观一脉相承。近年来,研究证实环境因素通过基因的重返表达对表观遗传产生影响,同时疗养因子参与机体的生物节律调控不断深入,机体对自然疗养因子的应答反应是受到多种因素的影响,包括机体状态、疗养因子的种类、性质、作用剂量、应用时间、季节及个体差异等。本文主要对自然疗养因子的作用机制及对机体的作用等方面进行总结。     

ω-3多不饱和脂肪酸对急性肺损伤炎症反应及免疫功能影响的机制研究进展

烧(创)伤、吸入性损伤、严重感染以及休克等疾病过程中,机体内多种炎性介质的释放,引发炎性细胞向肺组织迁移,激活呈级联放大的炎症反应,常常可造成以肺毛细血管内皮细胞和肺泡上皮细胞损伤为主的急性肺损伤(ALI),严重者短时间内可迅速发展为急性呼吸窘迫综合征(ARDS),病死率极高。ω-3多不饱和脂肪酸(ω-3PUFA)作为药理性免疫营养素的重要组成部分,目前已经超越了以往单纯提供能量、恢复正氮平衡的范畴,发挥着调控机体炎症反应、免疫功能的全面作用,并逐渐演变为现代危重性肺损伤治疗的重要组成部分。因此,本文就ω-3PUFA对ALI炎症反应及免疫功能影响的机制作一综述,旨在为临床治疗ALI提供理论依据。     

黄连素对人胃癌细胞SGC7901凋亡影响的研究

目的:探讨黄连素对人胃癌细胞SGC7901凋亡的影响。方法:MTS法检测不同浓度的黄连素(100、150、200μmol/L)对胃癌细胞的抑制作用,Hoechst 33258染色检测不同浓度的黄连素(100、150、200μmol/L)对细胞凋亡的影响;Real Time Q-PCR检测胃癌细胞中Cleaved Caspase-3、Bcl-2、Bax的mRNA表达;Western blot检测胃癌细胞中Cleaved Caspase-3、Bcl-2、Bax的蛋白表达。结果:不同浓度的黄连素能显著降低人胃癌细胞SGC701活性(P<0.05,P<0.01),促进其凋亡,升高Cleaved Caspase-3、Bax的mRNA和蛋白表达水平(P<0.05,P<0.01),降低Bcl-2的mRNA和蛋白表达水平(P<0.05,P<0.01)。结论:不同浓度的黄连素可诱导胃癌细胞凋亡,其机制可能与升高Cleaved Caspase-3、Bax的表达,降低Bcl-2的表达有关。     

清热解毒、散风除翳法对细菌性角膜炎的作用机制研究

目的:观察清热解毒、散风除翳法对细菌性角膜炎模型鼠的疗效及其作用机制。方法:将60只Wistar大鼠随机分为5组,即模型组、小剂量组、大剂量组、对照组以及空白组，每组12只大鼠。采用细菌性角膜炎动物模型症状评定观察清热解毒、散风除翳法的疗效,通过细菌清除率、角膜细菌菌落计数,光学显微镜观察大鼠角膜的病理组织学改变,并采用流式细胞仪检测CD4+,CD8+及CD4+/CD8+的水平讨论清热解毒、散风除翳法的作用机制。结果:1)清热解毒、散风除翳法可降低细菌性角膜炎性反应状评分,提高细菌清除率,改善角膜基质层的病理结构;2)清热解毒、散风除翳法可提高细菌性角膜炎模型鼠外周血血CD4+、CD8+的表达水平,具有剂量依赖性(P<0.05),CD4+/CD8+比值相对稳定,各组间差异无统计学意义(P>0.05)。结论:清热解毒,散风除翳法能有效改善细菌性角膜炎,其作用机制可能与上调大鼠外周血中CD4+,CD8+含量有关。     

表面等离子共振技术在中医药研究中应用进展

利用表面等离子共振现象制备的生物传感技术具有无标记、灵敏度高、通量大、特异性强，样品耗损少等优点，在生命科学等众多学科中得到了广泛的关注和应用。近年来该技术也被运用在不少中医药研究的重要领域。本文简要介绍了表面等离子共振原理，总结并评述了在中医药新靶标发现、优效小分子寻找等方向的研究进展和前景。