黄连素对人胃癌细胞SGC7901凋亡影响的研究

目的:探讨黄连素对人胃癌细胞SGC7901凋亡的影响。方法:MTS法检测不同浓度的黄连素(100、150、200μmol/L)对胃癌细胞的抑制作用,Hoechst 33258染色检测不同浓度的黄连素(100、150、200μmol/L)对细胞凋亡的影响;Real Time Q-PCR检测胃癌细胞中Cleaved Caspase-3、Bcl-2、Bax的mRNA表达;Western blot检测胃癌细胞中Cleaved Caspase-3、Bcl-2、Bax的蛋白表达。结果:不同浓度的黄连素能显著降低人胃癌细胞SGC701活性(P<0.05,P<0.01),促进其凋亡,升高Cleaved Caspase-3、Bax的mRNA和蛋白表达水平(P<0.05,P<0.01),降低Bcl-2的mRNA和蛋白表达水平(P<0.05,P<0.01)。结论:不同浓度的黄连素可诱导胃癌细胞凋亡,其机制可能与升高Cleaved Caspase-3、Bax的表达,降低Bcl-2的表达有关。

Effects of Berberine on Apoptosis of Gastric Cancer Cell Line SGC7901

Objective:Current studies showed that berberine had a certain anti-tumor effect,but its antitumor mechanism did not have depth study yet.This study discussed that the influence of berberine on human gastric cancer cells SGC7901′s apoptosis.Methods:The inhibition of gastric cancer cells induced by different concentrations of berberine(150μM,200μM,250μM)was determined by MTS.Hoechst 33258 staining method detected the apoptosis of gastric cancer cells induced by different concentrations of berberine(100μmol/L,150μmol/L,200μmol/L).The mRNA and protein expressions of Cleaved Caspase-3,Bcl-2,and Bax of gastric cancer cells were assessed by Real Time Q-PCR and western blot.Results:Different concentrations of berberine could significantly reduce the human gastric cancer cells SGC701′s activity,promote its apoptosis,increase mRNA and protein expressions of Cleaved Caspase-3,Bax,and reduce the mRNA and protein expressions of Bcl-2.Conclusion:Different concentrations of berberine could reduce gastric cancer cells apoptosis.The mechanism might be elevated the expressions of Cleaved Caspase-3,Bax and reduced the expression of bcl-2.