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91.
Simon Vinitski Carlos Gonzalez Feroze Mohamed Tad Iwanaga Robert L. Knobler Kamil Khalili John Mack 《Magnetic resonance in medicine》1997,37(3):457-469
Our aim was to develop an accurate multispectral tissue segmentation method based on 3D feature maps. We utilized proton density (PD), T2-weighted fast spin-echo (FSE), and T1-weighted spin-echo images as inputs for segmentation. Phantom constructs, cadaver brains, an animal brain tumor model and both normal human brains and those from patients with either multiple sclerosis (MS) or primary brain tumors were analyzed with this technique. Initially, misregistration, RF inhomogeneity and image noise problems were addressed. Next, a qualified observer identified samples representing the tissues of interest. Finally, k-nearest neighbor algorithm (k-NN) was utilized to create a stack of color-coded segmented images. The inclusion of T1 based images, as a third input, produced significant improvement in the delineation of tissues. In MS, our 3D technique was found to be far superior to that based on any combination of 2D feature maps (P < 0.001). We identified at least two distinctly different classes of lesions within the same MS plaque, representing different stages of the disease process. Further, we obtained the regional distribution of MS lesion burden and followed its changes over time. Neuropsychological aberrations were the clinical counterpart of the structural changes detected in segmentation. We could also delineate the margins of benign brain tumors. In malignant tumors, up to four abnormal tissues were identified: 1) a solid tumor core, 2) a cystic component, 3) edema in the white matter, and 4) areas of necrosis and hemorrhage. Subsequent neurosurgical exploration confirmed the distribution of tissues as predicted by this analysis. 相似文献
92.
目的:探讨胃复春片治疗慢性胃病患者前后生长抑素内分泌细胞(D细胞)数量变化。方法:用免疫组化酶链亲合素过氧化酶复合技术(SABC)对患者治疗前后胃粘膜组织的D细胞染色,观察治疗前后D细胞的形态和数量变化。结果:经胃复春片治疗后,患者胃窦粘膜D细胞阳性数及强阳性率均明显下降,有显著意义。结论:胃复春片对慢性胃病患者生长抑素D细胞有调整作用。 相似文献
93.
A. Newman-Tancredi V. Audinot V. Jacques J. L. Peglion M. J. Millan 《Neuropharmacology》1995,34(12):1693-1696
The selective dopamine D3 receptor antagonist [3H](+)S 14297 ((+)-[7-(N,N-dipropylamino)-5,6,7,8-tetrahydro-naphtho(2,3b)dihydro,2,3-furane]), labelled to high specific activity (145 Ci/mmol), bound to cloned human dopamine D3 receptors but displayed negligible binding to cloned human D2 receptors. [3H](+)S 14297 exhibited rapid association and dissociation, high affinity saturable binding (Kd = 7.0 nM) and a competition binding profile highly correlated with that of [125I]iodosulpride (r = 0.98). 相似文献
94.
脐动脉血流速波、产前胎心监护及脐动脉血气分析与新生儿结局的对比研究 总被引:1,自引:0,他引:1
测定115例足月妊娠者(正常妊娠62例,高危妊娠53例)脐动脉血流速波(UmAS/D),同时作产前胎心电子监护(NST),其中37例于分娩时抽取脐动脉血作血气分析。对上述三种方法预测新生儿结局的效果进行比较。结果表明:UmAS/D、脐动脉血的pH值较NST对新生儿结局不良有较好的预测性,异常UrnAS/D与新生儿酸中毒有密切关系。 相似文献
95.
In this study, the behavioural response to dopamine D1-like receptor agonists (SKF 38393, SKF 81297 and SKF 77434) and D2-like receptor agonists (quinpirole and RU 24213), administered alone and in combination to rats treated repeatedly with electroconvulsive
shock (five ECS over 10 days) or sham, was tested. Agonist-induced behaviour was monitored by automated activity meters and
direct observation using a checklist scoring method. Repeated ECS (compared to sham controls) had no significant effect on
the behavioural response to SKF 38393 (7.5 mg/kg SC), SKF 81297 (0.2 mg/kg SC), SKF 77434 (0.1 mg/kg SC), quinpirole (0.1
and 0.25 mg/kg SC) or RU 24213 (0.3 mg/kg SC), when administered alone. In contrast, repeated ECS markedly increased locomotion
(activity counts and scores) induced by the non-selective dopamine agonist apomorphine (0.5 mg/kg SC) and by co-administration
of a D1-like agonist plus a D2-like agonist [SKF 38393 (7.5 mg/kg SC) plus quinpirole (0.25 mg/kg SC), SKF 81297 (0.2 mg/kg SC) plus quinpirole (0.1 mg/kg
SC), and SKF 77434 (0.1 mg/ kg SC) plus RU 24213 (0.3 mg/kg SC)]. This ECS-induced enhancement of dopamine-mediated behaviour
was observed for up to 3 weeks after cessation of ECS treatment. In addition, ECS also enhanced the locomotor response to
intra-accumbens SKF 38393 plus quinpirole (0.4 and 1.0 μg/side, respectively). These results provide evidence that the enhancement
of dopamine function by repeated ECS requires concomitant stimulation of both D1-like and D2-like receptors, and that this effect is long-lasting.
Received: 24 January 1997 /Final version: 5 March 1997 相似文献
96.
采用大蒜对焦炉工在不脱离生产情况下,进行为期半年的实验服用,观察其对生物膜损伤和细胞免疫功能的保护作用。结果表明:服用大蒜制剂后焦炉工的唾液酸(SA)和脂质过氧化(LPO)比服用前降低(P<0.01),谷胱甘肽过氧化物酶(GSH-Px)有所增高,但P>0.05,在细胞免疫方面,表现为酸性α-醋酸萘酯酶(ANAE)和活性E-花环%(Ea)的增高(P<0.01)。对照组的细胞免疫功能及生物膜损伤情况均无改善,提示:大蒜对焦炉工的生物膜和细胞免疫均有一定的保护作用。 相似文献
97.
Adorján F. Kovács Waleed Megahed Michael Scholz Robert Sader 《Mund-, Kiefer- und Gesichtschirurgie》2007,11(5):267-283
PURPOSE: The development of overall survival of a DOSAK (German-Austrian-Swiss Cooperative Group on tumours of the maxillofacial region) clinic's overall population comprising a time period of more than 20 years (1983-2004) should be assessed. At a cutoff date (January 1st, 1997), a change from a primarily surgically based to a consequent multi-modality treatment regimen was implemented. The periods of time before and after that change should be compared. METHODS AND PATIENTS: The data of the DOSAK registry entries on 1038 patients suffering from primary untreated oral and oropharyngeal carcinomas were updated with respect to follow-up and mortality data to achieve a 100% quality of follow-up. The end point (death) was reached in 67% of the overall population. Statistical analysis was carried out by the Trium Analysis Online corporation, Munich. RESULTS: The portion of female and older tumor patients increased, more than half of all tumor patients were clearly in stage IV of the disease at first referral. The portion of patients operated on persisted approximately (80%), the portion of additional treatment modalities could be increased considerably. The fact of a bony infiltration by the tumor and the operability remained highly significantly relevant for survival in multivariate analysis, despite of multi-modality treatment. The survival rate of the patients remained significantly dependent on the clinical stage of the disease in multivariate analysis but could be improved by 10% in the clinical stages II and III and in the patients who could not be operated on. All in all, the cutoff date was statistically relevant for survival in multivariate analysis, i.[Symbol: see text]e. the change in the treatment regimen had a verifiable positive effect on the survival of a unicentric overall population. CONCLUSION: Survival improvement in an overall population via change in treatment strategy is possible in relatively short time; the clinical stages II and III and the non-operable patients have the greatest benefit from a multi-modality treatment. 相似文献
98.
Momoko Ogawa Nobuhito Hirawa Takamasa Tsuchida Naomi Eguchi Yukari Kawabata Atsushi Numabe Hideyuki Negoro Rie Hakamada-Taguchi Kousuke Seiki Satoshi Umemura Yoshihiro Urade Yoshio Uehara 《Nephrology, dialysis, transplantation》2006,21(4):924-934
BACKGROUND: Otsuka Long-Evans Tokushima Fatty (OLETF) rats genetically develop diabetes which is associated with hypertension. In preliminary studies, urinary excretions of L-PGDS (lipocaline-type prostaglandin D synthase) increase before diabetic nephropathy obviously develops, and this may predict progression of renal injury following diabetes. In the present study, we attempted to define whether urinary excretions of L-PGDS behave as the predictor of development of diabetic nephropathy in OLETF rats. METHODS: We investigated alterations of urinary L-PGDS excretions during the establishment of diabetes and assessed the relationship between the L-PGDS excretions and renal function in OLETF rats. Furthermore, we treated OLETF rats with troglitazone and analysed the effects on L-PGDS metabolisms. Urinary L-PGDS was measured by immunoenzyme assay and the occurrence of L-PGDS and its mRNA in the kidney was assessed by immunohistochemistry and a PCR method. RESULTS: Urinary excretions of L-PGDS were significantly higher in OLETF rats than non-diabetic Long-Evans Tokushima Otsuka (LETO) rats. The excretions age-dependently increased in OLETF and this increase appeared to be due to increased glomerular permeability to L-PGDS. Messenger RNA and antigenicity of L-PGDS were demonstrated in renal tissue; however, the de novo synthesis of L-PGDS mRNA seemingly contributed to urinary L-PGDS excretions much less than glomerular filtration. Multiple regression analysis revealed that urinary L-PGDS was determined by urinary protein excretions, and not by high blood pressure per se. Conversely, urinary proteinuria in the established diabetic nephropathy was predicted by urinary L-PGDS excretions in the early stage of diabetes. CONCLUSIONS: Urinary excretions of L-PGDS are likely to reflect the underlying increase in glomerular permeability. This property may be useful to predict forthcoming glomerular damage following diabetes in OLETF rats. 相似文献
99.
D4S1647、D6S2414基因座在中国汉族、蒙古族、藏族的遗传多态性 总被引:3,自引:3,他引:0
目的 调查D4S1647、D6S2414基因座在中国汉族、蒙古族、藏族群体中的遗传分布规律。方法 采集308份血及唾液标本应用PCR技术,扩增产物用非变性聚丙酰胺凝胶电泳分离,银染显色分析。结果 两位点各群体基因型频率分布均符合Hardy-Weinberg平衡,每一位点等位基因频率分布在各群体间无显著差异;通过对10个汉族家系的遗传模式分析,证实了两位点等位基因传递遵循孟德尔遗传规律。结论 D4S1647、D6S2414基因座在中国汉族,蒙古族,藏族群体均具有高度遗传多态性。 相似文献
100.
利培酮治疗与细胞色素P4502D6/C188T酶基因多态性的关联分析 总被引:2,自引:0,他引:2
目的 研究利培酮临床效应的个体差异与其代谢酶细胞色素P4 5 0 2D6 (cytochromeP4 5 02D6 ,CYP2D6 )酶基因多态性的相关性。方法 对 88例符合CCMD 3精神分裂症诊断标准的患者和 96例健康对照者作病例 -对照分析。精神分裂症患者给予利培酮治疗 8周 ,用阳性和阴性症状量表 (posi tiveandnegativesymptomscale ,PANSS)评分评价利培酮疗效。采用聚合酶链反应扩增及限制性片段长度多态性 (PCR RFLP)技术对CYP2D6exonⅠ的C188T位点突变进行检测 ,分析利培酮临床效应与其主要代谢酶CYP2D6 /C188T酶基因多态性的相关性。结果 中国上海地区人群的CYP2D6 /C188T突变率(弱代谢型 )为 36 .3% ,病例组和正常对照组间基因型频率总体分布比较无显著差异 (χ2 =1.15 ,df=2 ,P >0 .0 5 ) ,两组间的等位基因频率之间比较也无显著性差异 (χ2 =0 .78,df=1,P >0 .0 5 )。进行性别及有否家族史分组后分析 ,亦无差异存在 ,且CYP2D6 /C188T突变与利培酮临床效应之间并无相关性 (χ2 =1.12 ,df=2 ;χ2 =0 .0 3,df=1,P >0 .0 5 )。结论 未发现中国人CYP2D6 /C188T多态性与利培酮临床效应的个体差异有相关性。 相似文献