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91.
The use of direct susceptibility testing from specimens has led to the fortuitous observation that penicillin-susceptible strains have larger inhibition zones for mecillinam than do β -lactamase producers. The current study was, therefore, undertaken to test 179 Staphylococcus aureus isolates for mecillinam susceptibility by Rosco Neo-Sensitabs and to compare the results with commonly used tests for β -lactamase production, i.e. size and character of penicillin inhibition zones, chromogenic cephalosporin (nitrocefin) tests and clover leaf assays. Agreement between methods was reached for 175 of 179 strains when disregarding the results of the nitrocefin tests, 88 isolates being found susceptible and 87 being found to be β -lactamase producers. All 88 susceptible isolates had mecillinam zones of >22 mm, with the great majority being >25 mm; double zones did, however, occur. The 87 β -lactamase producers had zones <14 mm or no zones. Four isolates presenting problems in had mecillinam zones of ≤20 mm and were without tapering growth at the penicillin inhibition zone edge. In conclusion, the size of the mecillinam inhibition zone is found to be a useful supplementary test in the clinically important distinction between β -lactamase-producing and non-producing isolates of S. aureus . 相似文献
92.
C.K. Biswas J.M. Ramos D.N.S. Kerr 《Clinica chimica acta; international journal of clinical chemistry》1981,116(3):343-347
The effect of heparin on plasma ionised calcium was studied by adding it in increasing amounts to whole blood from 10 normal subjects. There was no significant change in ionised calcium from the addition of 1 U/ml but a significant fall of 0.02 mmol/1 when 2 U/ml were added and a progressive further fall with increasing concentrations. Heparin from three different manufacturers produced similar results. The effect of heparinisation in vivo was studied during regular haemodialysis on 10 patients with chronic renal failure. Following intravenous injection of 10000 U of heparin there was a consistent and significant fall averaging 0.03 mmol/l. 相似文献
93.
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95.
82例高血压患者16项人格特质测验 总被引:1,自引:0,他引:1
研究目的应用卡特尔16项人格特质测验探讨原发性高血压患者的人格特征。研究背景高血压病是危害人类健康的常见病之一,发病率逐年上升,但其病因至今尚未阐明,故有必要从心理、人格方面来探讨高血压的发病原因,以利于高血压病的防治。研究方法本文对82例均由2名以上内科医师确诊为原发性高血压、且现在高压仍保持在20/12.7kPa以上的患者进行卡特尔16项人格特质测验,并与78例人群对照分析。结果高血压患者在C-稳定性、E-峙强性、Q-紧张性以及在双重人格因素类型中的适应与焦虑方面与正常对照组有显著差异(P<0.05)。结论心理-社会因素是促使高血压发生的重要因素,它对高血压病因的阐明有重要意义,同时也使我们越来越重视运用心理、行为疗法,如生物反馈、各种松驰疗法等去防治高血压。 相似文献
96.
Idziorek Khalife Billaut-Mulot Hermann Aumercier Mouton Capron Bahr 《Clinical and experimental immunology》1998,112(1):84-91
The chemoattractant cytokine IL-16 has been reported to suppress lymphocyte activation and to inhibit HIV-1 replication in acutely infected T cells. We have cloned and expressed human IL-16 in Escherichia coli and investigated whether the recombinant protein could regulate the level of lymphocyte apoptosis from HIV-1-infected subjects. After purification and refolding, only 2–10% of the recombinant cytokine was present in a biologically active homotetrameric form. This could explain the need for high concentrations of the bacterially derived IL-16 to induce significant inhibition of HIV-1 replication. Addition of IL-16 to unstimulated peripheral blood mononuclear cell (PBMC) cultures from HIV-1-infected subjects did not modify the observed level of spontaneous lymphocyte apoptosis. In contrast, IL-16 added to PBMC cultures stimulated with anti-CD3, anti-CD95 or dexamethasone reduced significantly the percentage of lymphocytes undergoing AICD. This effect was found to correlate with the ability of the cytokine to decrease CD95 expression on activated CD4+ T cells. Comparative studies on PBMC from healthy individuals indicated that the regulation of apoptosis levels by IL-16 is a complex phenomenon and could depend on the nature of the activator used and/or the immune status of lymphocytes tested. The outcome of CD4 cross-linking on T cells by various ligands is discussed in the context of the observed beneficial activities of IL-16 and its potential role in the treatment of HIV disease. 相似文献
97.
用PCR—RFLP和16SrDNA指纹图法分析幽门螺杆菌基因型 总被引:1,自引:1,他引:0
本文建立了PCR-RFLP和16srDNA指纹图法.对19株幽门螺杆菌(HP)进行基因型分析:HP尿素酶C基因的PCR扩增产物.分别用HindⅢ、HaeⅢ、AluⅠ酶切,结果显示:每个酶均将19株HP分为3种RFLP图谱.综合HindⅢ,HaeⅢ和AluⅠ酶切结果,19株HP分为10个酶切带型;PCR扩增HP标准株16SrRNA基因,地高辛标记制备550bp探针,19株HPDNA分别经HaeⅢ和EcoRⅠ酶切、电泳后,通过Southern杂交获16SrDNA指纹图,结果显示:HaeⅢ酶切分为14个杂交带型,EcoRⅠ酶切19株HP杂交带型均不同。本实验表明:上述两种方法重复性好,分群力高,可准确有效地对HP作出鉴定并将其分型。19株HP株间存在基因型差异。 相似文献
98.
Helene Z. Hill Maimu Ohanian George J. Hill Richard Winne 《Journal of surgical oncology》1987,34(4):264-267
Intraoperative radiation therapy (IORT) research is limited by the lack of small-animal models. We have implanted B16 melanoma into mouse kidneys, which we subsequently operated upon and irradiated with beta rays from a 90Sr ophthalmic applicator. The IORT has effectively prolonged survival and produced some cures. The strategy should be applicable to other murine tumors and to other internal implantation sites. 相似文献
99.
P16MTS1、P27KIP1基因在胆管癌组织中的表达研究 总被引:2,自引:0,他引:2
目的:探讨P16^MIS1,P27^KIP1基因与胆管癌临床病理的关系。方法:采用免疫组化方法,检测48例胆管癌和8例伴慢性炎症的胆管壁组织中P16^MIS1,P27^KIP1蛋白表达情况。结果:(1)胆管癌中P16^MIS1,P27^KIP1阳性表达率分别为47.9%和35.4%,而伴炎症的胆管组织阳性表达率为100%和87.5%;(2)P16^MIS1,P27KIP1蛋白表达与胆管癌的分化程度,有无淋巴结或远处转移以及临床分期呈显著相关性。结论:P16^MIS1,P27^KIP1多基因表达异常可能与胆管癌的发生发展有关,是衡量胆管癌预后的有用指标。 相似文献
100.
目的:构建含有目的基因kring5的真核植物细胞穿酸表达载体。方法:应用RT-PCR方法,从正常人肝脏组织中获得kringle5基因,测序后,再通过DNA重组技术,将kringle5基因片段克隆到真核植物细胞表达载体pBI121和pCAMBIA3301上。结果:得到的kringle5基因序列测定结果与文献相符,重组载体pB1k5和pC33k5经酶切鉴定正确,含有植物高效表达CaMV35S启动子。结论:重组载体pB1k5和pC33k5不仅可以在大肠杆菌中稳定复制,而且可以在植物细胞中表达。 相似文献