小鼠胸腺的胞外基质发育

目的；探讨胞外基质在胸腺发育中的作用及与Ｔ细胞发育分化的关系。方法：用抗纤粘连蛋白、层粘连蛋白、Ⅳ型胶原抗体，以免疫组织化学ＡＢＣ法对所用胸腺切片染色。结果：１３ｄ胎鼠胸腺内有点状、串珠状ＥＣＭ阳性区。胸腺内ＥＣＢ表达，从鼠胚１３ｄ到１９ｄ渐增强，主要分布于被膜、小叶间隔、血管壁及部分实质细胞间；以后渐砬弱且集中在血管壁。结论：ＥＣＭ表达的变化与胸腺细胞进入胸腺并在胸腺内发育分化最后释放人血有关。     

异种胸腺,骨髓联合移植诱导受体免疫耐受

异种胸腺、骨髓联合移植诱导受体免疫耐受胡军１贾赤宇２马文煜１陈璧２蔡振杰３陈志云４张戈４岳小红４（１第四军医大学微生物学教研室西安７１００３３２第四军医大学西京医院烧伤科３第四军医大学唐都医院心脏外科４第四军医大学学员一大队）关键词胸腺骨髓异种移植免...     

萎缩胸腺内网状上皮细胞的形态学观察

104例尸检萎缩胸腺组织,用PAP法进行抗角蛋白多克隆抗体检测及组织学观察,发现61例病理性重度萎缩胸腺中,网状上皮细胞有4种分布类型①正常分布,②小叶中心集中分布,③小叶内平均分布,④消减型.7例生理性萎缩胸腺,均为消减型.胸腺网状上皮细胞形态有具细长突起(皮质)及短突起(髓质)2型.萎缩胸腺内有60.7%呈网状上皮增生,尤以髓质区明显.有12例胸腺组织行扫描电镜观察,见胸腺重度萎缩呈疏网型、蜂巢型及消减型序列发生.     

低剂量辐射对小鼠移植肿瘤生长和肿瘤诱生的影响

本研究以Ｃ５７ＢＬ／６纯系小鼠皮下接种Ｌｅｗｉｓ肺癌细胞或接受４次１．７５ＧｙＸ射线全身照射诱发胸腺瘤为移植和诱生肿瘤实验模型。发现接咱Ｌｅｗｉｓ肺癌细胞前７５ｍＧｙ照射小鼠于停照后六个月胸腺瘤发生率为７１．４％，而于１．７５Ｃｙ照射前６或１２小时接受７５ｍＧｙ照射小鼠均无胸腺瘤发生。提示低剂量辐射对移植肿瘤生长和高剂量辐射诱发胸腺瘤均有抑制作用。本文简要讨论了抑制作用的可能机理。     

恶性胸腺瘤的CT表现

复习９例经手术病理证实的恶性胸腺瘤的ＣＴ表现，着重讨论肿瘤的大小、形态、边缘、密度及肿瘤与邻近组织结构的关系等。认为胸腺瘤如出现下列征象提示恶性可能：１．肿瘤较大（最大径＞９ｃｍ），内有大小不等的低密度区。２．肿瘤形态不规则，边缘毛糙，凹凸不平。３．肿瘤与邻近胸膜、大血管、心脏分界不清。如出现下列现象之一则恶性诊断基本成立：１．肿瘤在短期内明显增大。２．肿瘤已侵犯肺实质。３．出现转移灶。     

胸腺修饰诱导同种异体静脉移植的免疫耐受研究

Objective To investigate the possibility of inducing immune tolerance to vein trans-plantation in rats by intrathymic injection of allogene. Methods MHC antigen extracted from splenic cells of donor Wistar rats was intrathymically injected to recipients SD rats, and donor Wistar femur vein was transplanted after 2 weeks. Forty-eight SD rats were randomly divided into 4 groups: group A ( femur vein autograft) ,group B ( femur vein allograft) ,group C ( femur vein allograft with use of immunosuppressant), group D (intrathymus injection). Imaging, histology and immunological assays of these groups were carried out 2 weeks after the transplantation. Results Histologic parameters that were tested were better in group D than those in group B. The concentration of serum IFN-γ in group B was significantly higher than that in groups A, C and D [ ( 86. 707±10.928 ) ng/L vs (29.328±4. 170), (69.076±8.059) and (63.355± 4.895) ng/L respectively, P < 0.05 ]. The concentration of serum IL-4 in group B was obviously lower than in groups C and D [ (23.656±3.369) ng/L vs (29.425±4.174) and ( 31.000±4.659) ng/L re-spectively,P < 0.05 ]. Conclusion Intrathymus injection of allogenic antigen might induce specific im-mune tolerance to femur vein transplantation in rats.