人乳头瘤病毒16型DNA转化人胚肺细胞的实验研究

人乳头瘤病毒(HPV)的16,18,31,33型等与宫颈癌的发病有关,其中HPV16与宫颈癌关系密切。为进一步研究HPV16的致癌性,我们用克隆的HPV16 DNA(2μg/10~5细胞)转染体外培养的人胚肺细胞,并进行了细胞存活时间、血清依赖性、着壁依赖性、间接免疫酶检测、HPV16 DNA、同源序列检测、染色体核型等生物学的研究。结果表明,转染细胞存活时间延长、在软琼脂培养基中形成集落、HPV16特异抗原得以表达、HPV16 DNA的同源序列存在于细胞中。表明本实验用HPV16DNA转染的人胚肺细胞具备转化细胞的某些特征,HPV16有使人胚肺细胞转化的作用。     

病理性损伤因素对肾小管上皮细胞表型转化的影响

目的 :观察低血清、高糖、高蛋白刺激下肾小管上皮细胞向间叶性细胞的表型转化 ,探讨损伤的肾小管上皮细胞在肾小管间质纤维化中的作用。方法 :以体外培养的人近端肾小管上皮细胞为对象 ,分别用含低血清(0 .2mol·L-1小牛血清 )、高葡萄糖 (4.5 g·L-1)、高白蛋白 (15 g·L-1)的培养液培养 7d ,透射电镜检测细胞形态变化 ;免疫组化和Westernblot检测细胞表型改变 ,包括CK、Vimentin、α SMA、Ⅰ和Ⅲ型胶原 ,原位杂交检测Ⅰ型胶原mRNA表达 ,同时检测细胞TGFβ1蛋白表达。 结果 :低血清、高糖、高蛋白直接刺激下 ,肾小管上皮细胞出现明显形态学改变 ,包括细胞变为长梭形 ;透射电镜下细胞内线粒体明显减少 ,粗面内质网明显增加 ,并出现actin样微丝。免疫组化染色和Westernblot显示CK表达明显减弱 ,Vimentin、Ⅰ、Ⅲ型胶原、α SMA和TGFβ1表达增强。原位杂交显示Ⅰ型胶原mRNA表达增强。结论 :低血清、高糖、高蛋白可直接引起人近端肾小管上皮细胞TGFβ1表达增加 ,并发生向间叶性细胞的表型转化     

~3H-TdR掺入法测定NHG-1脾淋巴细胞转化功能

本文报告用~3H-TdR掺入法测定本室的NC系正常裸小鼠和荷人脑胶质瘤裸小鼠(NHG-1)的脾淋巴细胞转化功能。结果提示:正常的纯合子(nu/nu)T细胞缺乏,B细胞功能均正常;杂合子(nu/ )的T和B细胞功能均正常;荷瘤鼠(NHG-1,30代)除了T细胞缺乏,B细胞功能亦受到抑制。     

中西结合治疗椎动脉型颈椎病62例临床观察

1 临床资料 本组62例中,男38例,女24例;年龄最大67岁,最小21岁,平均36岁;病程最长8 a,最短2个月,平均15个月.全部病例均有眩晕症状,常在颈部旋转或屈伸时出现或加重,伴颈枕部不适或疼痛,颈肌僵硬.伴耳鸣32例,伴恶心或呕吐23例,伴上肢麻木感15例.     

Benign giant cell tumor of bone with osteosarcomatous transformation (”dedifferentiated” primary malignant GCT): report of two cases

 It is not uncommon for sarcomatous transformation of giant cell tumor (GCT) of bone to occur after radiation, but rarely does malignant transformation occur spontaneously, with less than 15 cases reported up to 1995. Only four of these cases have been documented in detail. We report two additional cases of GCT of bone spontaneously transforming or ”dedifferentiating” into osteosarcoma without radiation therapy. The first case is absolutely unique and most interesting in that the dedifferentiation process occurred in one of multiple GCT lung metastases 6 years after successful eradication of a primary tibial tumor. The right lung was resected due to development of a large tumor, and at pathologic examination, demonstrated several small nodules of conventional GCT and a much larger, 14-cm mass composed of a mixture of GCT and high-grade osteosarcoma. The second case involved a physician, who had a large tumor in the sacrum with vague symptoms for 8 years. Open biopsy revealed conventional, benign GCT of bone with a secondary aneurysmal bone cyst. Complete curettage 2 weeks later revealed, in addition to areas of conventional, benign GCT a second component of very high grade osteosarcoma. Both patients died less than 1.5 years from diagnosis. This report of osteosarcomatous transformation of a conventional GCT of bone strengthens the theory that there is a mesenchymal cell line in GCT that may spontaneously tansform to sarcoma.     

Effect of “Re Du Qing” on the activation,proliferation and membrane fluidity of lymphocytes

Summary Effects of Chinese Medicinal Preparation “Re Du Qing” (RDQ) on the activation, proliferation and membrane fluidity of T lymphocytes from human peripheral blood Were studied by means of³H-TdR incorporation and DPH fluorescence polarization. The results showed that “RDQ” can:1) significantly inhibit the activation of T lymphocytes; 2) restrain the proliferation of activated T lymphoblasts in the presence of exogenous interleukin-2 (IL-2); and 3) increase the membrane fluidity of T lymphocytes and antagonize the decreased fluidity of lymphocyte membrane mediated by Con A or PHA. The functional abnormalities of T lymphocytes in some autoimmune diseases such as arthritis and the usefulness of RDQ in the treatment of these diseases were also discussed.     

The Construction fo Hybrid Plasmid pBR322:RSF1010

The plasmids pBR322 and RSF1010,extracted from E.coli HB802 and E.coliC600 respectively,were treated with restrictive endonuclease EcoRI andligated with T4 DNA Iigase.C600 strains were then transformed with thehybrid plasmid(pSMMl),and thus253transformants were obtained.Thefigures of gel electrophoresis and electromicrograph proved that pSMMlwas a pBR322:RSF1010 composite hybrid.In E.coli C600 pSMMl obtainedresistance against ampieilline(Ap),tetracycline(Tc)and streptomycin(Sm).The orientation of pBR322 and RSF1010 in hybrid pSMMl and the failureof introduction of this hybrid into P.putida AC10 were discussed.     

A Drosophila melanogaster hobo-white+ vector mediates low frequency gene transfer in D. virilis with full interspecific white+ complementation

Transformation of a Drosophila virilis white mutant host strain was attempted using a hobo vector containing the D. melanogaster mini-white⁺ cassette (H[w⁺, hawN]) and an unmodified or heat shock regulated hobo transposase helper. Two transformant lines were recovered with the unmodified helper (HFL1), one containing only the white⁺ marked vector, and a sibling line containing the vector as well as an HFL1 helper integration. An approximate total transformation frequency of 1% is deduced. A high frequency of wing and eye morphology mutants were also observed, suggesting that hobo may have mobilized a related element in D. virilis. The data reaffirms a relatively low transformation vector activity for the hobo transposon in D. virilis; however, nearly full interspecific expression of the white⁺ marker supports its possible function in other species as well.     

煤焦沥青烟雾提取物的细胞毒性和遗传毒性研究

采用人外周血淋巴细胞非程序DNA合成(UDS)试验和人胚肺成纤维细胞转化试验,测试了煤焦沥青烟雾提取物(ECTPF)对人体细胞的细胞毒性和遗传毒性。UDS试验结果表明,ECTPF可使淋巴细胞UDS值明显增加,并有剂量一反应关系。引起半数淋巴细胞死亡的浓度(LC50)为33.8μg/ml。细胞转化试验表明,ECTPF能诱发人胚肺成纤维细胞明显的形态学转化,且转化细胞具有部分恶性转化细胞的特性。引起半数人胚肺成纤维细胞生长抑制的浓度为41.3μg/ml。实验结果提示,ECTPF是一种具有细胞毒性和遗传毒性的物质。     

门静脉海绵样变的手术治疗

目的 探讨门静脉海绵样变的手术治疗方式。方法 对9例门静脉海绵样变患者的14次手术方式进行回顾分析。结果 1例患者剖腹探查无法手术，其余13次手术中，分流手术5次，断流手术8次，对于再出血时间的对比分析，断流手术发生再出血时间长，并与断流的彻底性有关。结论 对于门静脉海绵样变的手术方式以彻底的断流术为佳，最好行贲门周围血管离断 食道下段 胃底切除再吻合术。