全文获取类型
| 收费全文 | 86348篇 |
| 免费 | 6564篇 |
| 国内免费 | 5382篇 |
专业分类
| 耳鼻咽喉 | 569篇 |
| 儿科学 | 2281篇 |
| 妇产科学 | 1086篇 |
| 基础医学 | 15232篇 |
| 口腔科学 | 1771篇 |
| 临床医学 | 7184篇 |
| 内科学 | 13571篇 |
| 皮肤病学 | 1088篇 |
| 神经病学 | 6145篇 |
| 特种医学 | 1929篇 |
| 外国民族医学 | 31篇 |
| 外科学 | 5387篇 |
| 综合类 | 16639篇 |
| 现状与发展 | 25篇 |
| 一般理论 | 1篇 |
| 预防医学 | 4023篇 |
| 眼科学 | 1306篇 |
| 药学 | 9017篇 |
| 8篇 | |
| 中国医学 | 2626篇 |
| 肿瘤学 | 8375篇 |
出版年
| 2024年 | 86篇 |
| 2023年 | 696篇 |
| 2022年 | 1231篇 |
| 2021年 | 2384篇 |
| 2020年 | 1993篇 |
| 2019年 | 1921篇 |
| 2018年 | 1881篇 |
| 2017年 | 2090篇 |
| 2016年 | 2455篇 |
| 2015年 | 2585篇 |
| 2014年 | 4024篇 |
| 2013年 | 5642篇 |
| 2012年 | 4437篇 |
| 2011年 | 5552篇 |
| 2010年 | 4523篇 |
| 2009年 | 4732篇 |
| 2008年 | 5193篇 |
| 2007年 | 5506篇 |
| 2006年 | 5287篇 |
| 2005年 | 5093篇 |
| 2004年 | 4720篇 |
| 2003年 | 4290篇 |
| 2002年 | 3761篇 |
| 2001年 | 3433篇 |
| 2000年 | 2977篇 |
| 1999年 | 2531篇 |
| 1998年 | 2175篇 |
| 1997年 | 1802篇 |
| 1996年 | 1268篇 |
| 1995年 | 1039篇 |
| 1994年 | 771篇 |
| 1993年 | 502篇 |
| 1992年 | 359篇 |
| 1991年 | 264篇 |
| 1990年 | 220篇 |
| 1989年 | 142篇 |
| 1988年 | 120篇 |
| 1987年 | 87篇 |
| 1986年 | 71篇 |
| 1985年 | 109篇 |
| 1984年 | 84篇 |
| 1983年 | 50篇 |
| 1982年 | 51篇 |
| 1981年 | 49篇 |
| 1980年 | 27篇 |
| 1979年 | 28篇 |
| 1978年 | 17篇 |
| 1977年 | 11篇 |
| 1976年 | 10篇 |
| 1973年 | 6篇 |
排序方式： 共有10000条查询结果，搜索用时 187 毫秒
31.
Discoid lupus erythematosus (DLE) is the most common skin manifestation of lupus; however, the molecular mechanisms underlying DLE remain unknown. Therefore, we aimed to identify key differentially expressed genes (DEGs) in discoid lupus skin and investigate their potential pathways.To identify candidate genes involved in the occurrence and development of the disease, we downloaded the microarray datasets GSE52471 and GSE72535 from the Gene Expression Database (GEO). DEGs between discoid lupus skin and normal controls were selected using the GEO2R tool and Venn diagram software (http://bioinformatics.psb.ugent.be/webtools/Venn/). The Database for Annotation, Visualization, and Integrated Discovery (DAVID), Enrichr, and Cytoscape ClueGo were used to analyze the Kyoto Encyclopedia of Gene and Genome pathways and gene ontology. Protein-protein interactions (PPIs) of these DEGs were further assessed using the Search Tool for the Retrieval Interacting Genes version 10.0.Seventy three DEGs were co-expressed in both datasets. DEGs were predominantly upregulated in receptor signaling pathways of the immune response. In the PPI network, 69 upregulated genes were selected. Furthermore, 4 genes (CXCL10, ISG15, IFIH1, and IRF7) were found to be significantly upregulated in the RIG-I-like receptor signaling pathway, from analysis of Enrichr and Cytoscape ClueGo.The results of this study may provide new insights into the potential molecular mechanisms of DLE. However, further experimentation is required to confirm these findings. 相似文献
32.
《Molecular therapy》2022,30(6):2186-2198
- Download : Download high-res image (179KB)
- Download : Download full-size image
33.
Shafqat R. Chaudhry Ilana S. Lendvai Sajjad Muhammad Philipp Westhofen Johannes Kruppenbacher Lukas Scheef Henning Boecker Dirk Scheele Rene Hurlemann Thomas M. Kinfe 《Brain stimulation》2019,12(3):643-651
Objective
To assay peripheral inter-ictal cytokine serum levels and possible relations with non-invasive vagus nerve stimulation (nVNS) responsiveness in migraineurs.Methods
This double-blinded, sham-controlled study enrolled 48 subjects and measured headache severity, frequency [headache days/month, number of total and mild/moderate/severe classified attacks/month], functional state [sleep, mood, body weight, migraine-associated disability] and serum levels of inflammatory markers [inter-ictal] using enzyme-linked immunoassays at baseline and after 2 months of adjunctive nVNS compared to sham stimulation and suitably matched controls.Results
No significant differences were observed at baseline and after 2 months for headache severity, total attacks/month, headache days/month and functional outcome [sleep, mood, disability] between verum and sham nVNS. However, the number of severe attacks/month significantly decreased in the verum nVNS group and circulating pro-inflammatory IL-1β was elevated significantly in the sham group compared to nVNS. Levels of anti-inflammatory IL-10 were significantly higher at baseline in both groups compared to healthy controls, but not at 2 months follow-up [p?<?0.05]. Concentrations of high-mobility group box-1 (HMGB-1), IL-6, tumor-necrosis factor-α (TNF-α), leptin, adiponectin, ghrelin remained unchanged [p?>?0.05]. No severe device-/stimulation-related adverse events occurred.Conclusion
2 months of adjunctive cervical nVNS significantly declined the number of severe attacks/month. Pro-inflammatory IL-1β plasma levels [inter-ictal] were higher in sham-treated migraine patients compared to verum nVNS. However, pro- [IL-6, HMGB-1, TNF-α, leptin] and anti-inflammatory [IL-10, adiponectin, ghrelin] mediators did not differ statistically. Profiling of neuroinflammatory circuits in migraine to predict nVNS responsiveness remains an experimental approach, which may be biased by pre-analytic variables warranting large-scale biobank-based systematic investigations [omics]. 相似文献34.
35.
目的:分析组蛋白去乙酰化酶抑制剂TSA处理前后,食管癌细胞株EC109中差异表达的非编码RNA(ncRNA)和mRNA表达谱,初步预测与TSA作用相关的ncRNA和mRNA。方法:采用MTT、细胞周期等方法检测TSA的效应;运用应用芯片技术分别检测TSA处理前后EC109细胞中ncRNA和mRNA表达谱变化,并对差异ncRNA和mRNA表达谱进行整合分析。结果:TSA以剂量时间依赖方式抑制细胞增殖,细胞周期阻滞和凋亡的发生。芯片检测共发现461个ncRNA和758个mRNA显著性差异表达,生物信息学分析显示差异涉及红比霉素、阿霉素代谢过程、氧化还原、核小体的装配、染色质结构组织和端粒的结构组织等。通过数据库分析预测及整合分析,得到了361个ncRNA-mRNA靶基因对。结论:差异表达的ncRNA和mRNA对TSA的生物学效应密切关联,为进一步研究基因功能及其在肿瘤中的生物学意义奠定基础。 相似文献
36.
37.
38.
39.
40.
《Molecular therapy》2020,28(6):1432-1441
- Download : Download high-res image (155KB)
- Download : Download full-size image