外源性睾酮对离体家兔阴茎海绵体的影响(英文)

AIM: To study the effects of exogenous excess of testosterone on the constricting effect of phenylephrine and en-dothelium-dependent and -independent relaxing effects of different agonists in the corpus cavemosum penis (CCP). METHODS: Specimens of the CCP were obtained from rabbits testosterone for 1 and 2 months and untreated for 2 months after testosterone-treatment for 2 months. Preparations were mounted between two parallel platinum electrodes in organ baths. Responses to phenylephrine, car-bachol, and sodium nitroprusside were obtained by adding the reagent cumulatively to the bath. RESULTS: The phenylephrine-induced contractions were decreased with no change in agonist potency (pD2 value) after both 1 and 2 month testosterone-treatment and did not return to control values in corpus cavernosum obtained from rabbits untreated for 2 months after testosterone-treatment for 2 months. Testosterone treatment for 1 or 2 months increased the endothelium-dependent relaxations induced by carbachol and decrea     

淫羊藿黄酮精制方法的建立

目的建立中药淫羊藿总黄酮的精制方法和质量控制标准.方法以淫羊藿苷的含量为指标,应用正交试验设计筛选淫羊藿总黄酮的最佳精制工艺;分光光度法测定总黄酮含量.结果分光光度法测定提取产物中主要活性成分淫羊藿总黄酮的含量平均为54%以上.结论本研究所建立的淫羊藿总黄酮提取工艺及质量控制方法切实可行、成本低廉.     

广金钱草抑制一水草酸钙结晶生长有效成分的研究

本文对广金钱草多糖类进行了提取和精制,并通过电导法观察精制前后对一水草酸钙结晶生长的抑制作用,发现精制后比精制前的作用更强。     

静脉输入高浓度氯化钾快速纠正心脏瓣膜替换术后早期低钾…

瓣膜替换术后早期低血钾较为多见，并常常带来严重的后果，需要快速予以纠正。而传统的补钾方法，难以做到此点，自１９９０年以来，我们对１１２例换瓣术后的病人行高浓度补钾以快速纠正术后低钾血症，收以了较好的疗效。实践证明，将２ｇ氯化钾溶于１００ｍｌ液体中静脉滴入，时间超过１ｈ是非常安全的，高浓度补钾以快速纠正低钾血症这一方法，有益于换瓣术后早期低钾的病人。     

头针配合精制蝮蛇抗栓酶治疗急性脑梗塞100例

我院收治经CT确诊的急性脑梗塞患者100例,采用头针配合精制蝮蛇抗栓酶治疗,疗效明显优于单用精制蝮蛇抗栓酶组。现报道如下:1 临床资料1.1 诊断标准:参照1986年全国第二次脑血管病会议通过的诊断标准,并经CT确诊。1.2 一般情况:选择发病4天以内的急性脑梗塞患者180人,其中治疗组的100例来自本院1993年至1995年1月住院者;对照组的80例,来自本市市级西医院神经内科同期住院病人。治疗组:男56例,女44例;年龄45～83岁,平均59岁,夜间     

Altered expression of renal bumetanide-sensitive sodium-pota-ssium-2 chloride cotransporter and Cl- channel -K2 gene in angiotensin Ⅱ-infused hypertensive rats

Background Little information is available regarding the effect of angiotensin Ⅱ (Ang Ⅱ) on the bumetanide-sensitive sodium-potassium-2 chloride cotransporter (NKCC2), the thiazide-sensitive sodium-chloride cotransporter (NCC), and the Cl- channel (CLC)-K2 at both mRNA and protein expression level in Ang Ⅱ-induced hypertensive rats. This study was conducted to investigate the influence of Ang Ⅱ with chronic subpressor infusion on nephron-specific gene expression of NKCC2, NCC and CLC-K2.Methods Sprague Dawleys rats were treated subcutaneously with either Ang Ⅱ (100 ng·kg-1·min-1 ) or vehicle for 14 days. Expression of NKCC2, NCC and CLC-K2 mRNA in kidneys was determined by real time polymerase chain reaction (PCR). Western blotting analysis was used to measure NKCC2 and NCC protein expression. Results Ang Ⅱ significantly increased blood pressure and up-regulated NKCC2 mRNA and protein expression in the kidney. Expression of CLC-K2 mRNA in the kidney increased 1.6 fold (P<0.05).There were no changes in NCC mRNA or protein expression in AngII-treated rats versus control.Conclusions Chronic subpressor Ang Ⅱ infusion can significantly alter NKCC2 and CLC-K2 mRNA expression in the kidney, and protein abundance of NKCC2 in kidney is positively regulated by Ang Ⅱ. These effects may contribute to enhanced renal Na+ and Cl- reabsorption in response to Ang Ⅱ. Chin Med J 2005; 118(23):1945-1951     

凹凸棒对盐酸小檗碱吸附作用考察

目的：考察凹凸棒对中药水煎提取液中盐酸小檗碱的吸附影响和对无效成分的吸附清除作用，并与自然静置沉淀法进行对比研究。方法：以水煎提取液中总固体物量和盐酸小檗碱含量作为评价指标，分别用不同量的普通凹凸棒和酸化凹凸棒处理黄连、一清颗粒水煎提取液，评价其吸附沉淀效果，平行与自然静置沉淀法进行比较。结果：凹凸棒对无效成分具有明显的吸附清除作用，对盐酸小檗碱也有一定的吸附影响，酸化凹凸棒沉淀去除无效成分的效果优于普通凹凸棒。结论：酸化凹凸棒作为中药水煎提取液中盐酸小檗碱精制提取的吸附沉淀剂，尚需进一步进行表面结构修饰研究。     

大孔吸附树脂法分离精制白芍的研究

目的：通过对10种大孔吸附树脂筛选,寻找适用于分离精制白芍的树脂种类及工艺条件。方法：以芍药苷的静态、动态吸附量和脱附率为指标筛选最佳树脂,通过单因素试验确定分离精制白芍的优选操作条件。结果：D-101-1树脂具有较好的吸附性能和解吸效果。吸附完全后,先以水洗脱,除去杂质,再以70%乙醇洗脱。结论：D-101-1树脂综合性能良好,适用于白芍的分离精制。     

大孔吸附树脂对黄连解毒汤的精制作用研究

目的:研究大孔吸附树脂对黄连解毒汤的精制作用。方法:采用薄层扫描及分光光度法,测定经过树脂前后提取液中小檗碱、栀子苷、总生物碱含量的变化,优选树脂种类、吸附及解析条件。结果:YWD-09D大孔吸附树脂适合黄连解毒汤的精制。树脂的吸附容量为1∶1.3;上柱液浓度为0.4 mg/ml,pH值为4,流速为2 ml/min,树脂径高比为1∶20;洗脱剂为4倍柱体积的90%乙醇。结论:YWD-09D大孔吸附树脂及其优选出的条件适合黄连解毒汤的精制。     

精制E型肉毒类毒素免疫原不同配制比例实验

目的为获得高效价的马血浆，选择最佳E型肉毒类毒素免疫原配制比例。方法用3种不同配制比例配制E型肉毒类毒素免疫原，免疫原1、2、3分别为V（羊毛脂）：V（液体石蜡）：V（精制E型肉毒类毒素抗原）=1：2：6，1：2：3，1：2：2。将24匹马分为3组，分别用3种免疫原按常规法免疫，采用小鼠中和试验方法检测马血浆效价。结果用免疫原2免疫的马匹，马血浆平均效价比用免疫原1、3平均效价提高了36．3％，且马匹表现状况好于免疫原1、3。结论免疫原2的配制比例使用安全、稳定性良好、马匹免疫效果较好，为目前抗毒素生产用免疫原最佳配制比例。