全科医疗质量评价指标体系的研究

本研究采用改进的Delphi法，经过两轮专家咨询，建立一套合理的全科医疗质量评价指标体系。两轮咨询专家的权威程度较高，其平均值分别为0．7634，0．7378。专家意见的协调系数分别为：0．122，0．231。最终确定全科医疗评价指标体系共有指标146项，其中一级指标4项，二级指标30项，三级指标112项。其中服务内容、服务满意度、效果效益、卫生经济4项一级指标的权重系数分别为0．2416、0．2646、0．2453、0．2485。     

间接测热法和反向Fick氏法测定心脏手术病人术后氧耗量的比较

目的 采用反向Fick氏法和间接测热法测定心脏手术后病人的全身氧耗,并比较两种测量方法的相关性和准确性。方法8例心脏手术术后病人,分别在入ICU后2h和6h时同时采用反向Fick氏和间接测热法测定病人的全身氧耗量。结果 间接测热法和反向Fick法测得氧耗分别是(16±30)ml·min-1·m-2和(127±23)ml·min-1·m-2,前者的测定结果显著高于后者(P<0.01)。相关分析显示两者有较好的相关性(r=0.92,P<0.01)。采用Bland和Altman统计分析提示两种测定结果的平均偏离值为(35.5±13.4)ml·min-1·m-2,其95％的分布范围是(9～62)ml·min-1·m-2,提示两种测量结果间的一致性较差。结论 两种方法测定心脏病人术后全身氧耗的结果有明显差异,其中反向Fick氏法的测定结果误差大、准确性差,而间接测热法是较好的临床选择。     

52例电烧伤的治疗分析

目的：总结电烧伤的治疗方法。方法：对52例资料齐全的电烧伤病人的治疗方法及疗效进行回顾性分析。结果：单纯电火花烧伤的治疗和一般烧伤相同，电击伤多为早期清创，植皮覆盖创面。结论：电烧伤创面根据情况可有多种治疗方法：包括单纯换药，湿润烧伤膏，中厚皮片移植，皮瓣移植等。     

Using Computer Simulated Results of a Bulk Drug Substance Assay to Determine Acceptance Criteria for Method Validation

Purpose. To determine the statistical variability expected for a well designed HPLC assay of a bulk drug substance (BDS). The results are used to develop appropriate acceptance criteria for a method validation protocol as well as to evaluate the level of uncertainty expected for assay results using a variety of sampling/injection schemes. Methods. Computer simulation was used to generate a large quantity of data and the variability of the mock results was evaluated. Error propagation was also calculated, whenever possible, to confirm results obtained from the simulations. Results. Protocol acceptance criteria were developed that were consistent with the expected variability for data resulting from the execution of the validation protocol. In certain cases simulations provided the only avenue of obtaining results that could not otherwise be readily determined. Conclusions. Computer modeling can be used to obtain suitable acceptance criteria for validation results which are consistent with method variability. This is particularly significant in the case of linearity where it has been difficult to develop acceptance criteria based on anything other than analyst intuition and experience. Assay simulations clearly demonstrated that the variability expected for a typical BDS assay is large relative to the average specification range and therefore little insight about relative purity can be gained comparing individual passing assay results.     

一种鉴别肥大细胞表型的复合染色法

运用ABC-爱先蓝-PAS复合染色法,分别对大鼠肝,胃、肠中不同表型肥大细胞即结缔组织肥大细胞(CTMC)和粘膜肥大细胞(MMC)进行鉴别染色实验。结果表明:肝汇管区MC呈棕褐色;胃粘膜上皮及粘膜下结缔组织内MC分别被染成蓝色和棕褐色;肠粘膜固有层MC呈蓝色.通过不同颜色反应从而确定其MC表型。本实验还通过对染色顺序。固定液选择等的多次摸索。取得了较好的染色效果,与其它检测方法的结果相一致,但试剂来源方便,操作简易。一般实验室即可进行。     

Improved sib-pair linkage test for disease susceptibility loci

An improved sib-pair test for linkage is introduced which is superior to the previously proposed tests. The test is derived from the standard chi-squared goodness of fit statistic by restricting the alternative hypothesis to the genetically possible. Critical values are given and exact power comparisons are made with the previously proposed tests. The new test is shown to be more powerful for finite samples as well as being asymptotically uniformly most powerful. © 1993 Wiley-Liss, Inc.     

孔边裂纹J积分失效评定曲线

计算了单边椭圆缺口试件（ＳＥＮＣＰ）高应变区裂纹的Ｊ积分失效评定曲线（ＦＡＣ），考察了影响ＦＡＣ的几种因素。结果表明：在外加载荷与塑性屈服载荷之比（Ｌｒ）１．０时，用Ｒ６通用失效评定曲线进行结构高应变区裂纹断裂评定与疲劳剩余寿命估算是安全的。     

Exteroceptive suppression periods in jaw-closing muscles. Variability and relation to experimental pain and sustained muscle contraction

The duration of the late exteroceptive suppression period (ES₂) of temporal muscle EMG activity has been reported to be reduced in patients suffering from chronic tension-type headache. Methods of recording and analysing ES₂ have varied between centers and reproducibility of results within subjects , although insufficiently studied, has generally been poor. ES₂ was investigated in 30 healthy subjects, using a computerized technique of recording, rectifying and averaging the EMG signals. Hour to hour and week to week variations of ES₂ durations were calculated, and the influence of pain during a cold pressor test and of sustained muscle contraction on ES₂ durations was investigated. The intra-individual variation of ES₂ durations was 16.0% from hour to hour and 20.7% from week to week. The inter-individual variation was 36.7%. The present method for analysis of ES₂ periods proved to be reliable, as the intra-observer variation was 4.2% and the inter-observer variation 4.6%. ES₂ periods were significantly shorter on the first compared to the second day of examination ( p = 0.006) and during experimental pain ( p = 0.0005). We recommend the use of the computerized averaging technique in future studies and caution against the dependence of results upon factors such as conditioning and pain.     

广东78种食物的不溶性膳食纤维含量分析

本文采用中性洗涤剂法测定了广东78种食物的不溶性膳食纤维含量,结果提示,不溶性膳食纤维的平均含量:干豆坚果类为7.12％,鲜果类为1.93％,根茎类为1.55％,叶菜类为1.31％,粮谷类为1.27％,茄瓜类为1.15％。其中干豆坚果类与茄瓜类、粮谷类、叶菜类、根茎类和鲜果类的含量差别极显著(P<0.01),而后五类食物的含量相互间差别不显著(P>0.05)。粮谷类的变异系数较大(CV:142％)。其中以加工较深的米面及其制品较低(0.31％—0.72％),而未深加工的玉米、薏米的含量则高(2.48％—6.08％)。     

扶正健脾汤配合环磷酰胺对荷瘤小鼠的实验研究

用环磷酰胺对荷瘤小鼠进行化疗同时配合扶正健脾汤进行治疗的疗效观察,证明扶正健脾汤具有减轻环磷酰胺的副作用,其表现为实验组小鼠的胸腺系数增大;脾内 IgG、IgM 阳性抗体形成细胞增多;肝内巨噬细胞的吞噬功能加强。提示扶正健脾汤具有拮抗环磷酰胺对骨髓、胸腺和脾脏的免疫抑制作用,为该方剂治疗肿瘤的临床应用提供实验依据。